Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-EGFP-ID (#RDB08527)

Expression clone of GFP under the control of CAG promoter, HisD resistance.

Alternative name stock no 10504
Clone info. Expression clone of GFP under the control of CAG promoter, HisD resistance.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 6.7 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB08527z.seq
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
MTAに書く付加的使用条件:
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08527 pPyCAG-EGFP-ID DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPyCAG-EGFP-ID was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08527).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08527_A4Fo.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB08527_A4Foa.seq check
>08527_08527_A4Fo_pAxCA_F1_A05_02.ab1
    1 TGTCCAGCGT CTTCTCAGTT CATGCCTTCT TCTTTTTCTA CAGTTCTGGG CAACGTGCTG
   61 GTTATTGTGG GAAACAAGCA GGTTTGGCAA AGAATTCCTC GAGATCCATT GTGGTTCAGT
  121 GCGGCCGATC CCCGGGTACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC
  181 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG
  241 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
  301 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG
  361 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
  421 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC
  481 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC
  541 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC
  601 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC
  661 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC
  721 GACGGCCCCG TGCTGCTGCT CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA
  781 AGACCCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA AGTTCGTGAC CGCCGCCCGG
  841 GATCACTCTC GGCATTGGAC GAGCTGTTAC AAGTTAAAGG CGGCCGGGCC GCAACTGGGC
901 CACAATGGGA TTCTC
//
Primer: IRES-F
Sequence file: RDB08527_A4Fob.seq check
>08527_08527_A4Fo_IRES-F_B05_05.ab1
    1 CTGCCCTCGG GCCTTTAATG GTGGTTTAGT CGAGGTTAAA AAACGTCTAC GCCCCCCGAA
   61 CCACGGGGAC GTGGTTTTCA AAAAGAAAAA CACGATGATA ATATGGCCAC AACCATGAGC
  121 TTCAATACCC TGATTGACTG GAACAGCTGT AGCCCTGAAC AGCAGCGTGC GCTGCTGACG
  181 CGTCCGGCGA TTTCCGCCTC TGACAGTATT ACCCGGACGG TCAGCGATAT TCTGGATAAT
  241 GTAAAAACGC GCGGTGACGA TGCCCTGCGT GAATACAGCG CTAAATTTGA TAAAACAGAA
  301 GTGACAGCGC TACGCGTCAC CCCTGAAGAG ATCGCCGCCG CCGGCGCGCG TCTGAGCGAC
  361 GAATTAAAAC AGGCGATGAC CGCTGCCGTC AAAAATATTG AAACGTTCCA TTCCACGCAG
  421 ACGCTACCGC CTGTAGATGT GGAAACCCAG CCAGGCGTGC GTTGCCAGCA GGTTACGCGT
  481 CCCGTCTCGT CTGTCGGTCT GTATATTCCC GGCGGCTCGG CTCCGCTCTT CTCAACGGTG
  541 CTGATGCTGG CGACGCCGGC GCGCATTGCG GGATGCCAGA AGGTGGTTCT GTGCTCGCCG
  601 CCGCCCATCG CTGATGAAAT CCTCTATGCG GCGCAACTGT GTGGCGTGCA GGAAATCTTT
  661 AACGTCGGCG GCGCGCAGGC GATTGCCGCT CTGGCCTTCG GCAGCGAGTC CGTACCGAAA
  721 GTGGATAAAA TTTTTGGCCC CGGCAACGCC TTTGTAACCG AAGCCAAACG TCAGGTCAGG
  781 CCAGCGTCTC GGACGGCGCG GCTATCGATA TGCCAGCCGG GCCGTTCTGA AGTACCTGGT
  841 GATCGCAGAC AGCCGGCGCA ACACCCGGAA TTTCGTTGCT TCTGACCTGC TCTCCCAAGG
  901 CTGAGCACGG GCCCGGGATT TCCCAGGTGA TTCCTGCTTG ACGCCCTGAT GCTGACCATT
  961 GCCCGCAAGG TGGGCGGGAG GGCGGGTAGA ACGTTCAACC TGGCGGAAAC TGCCGGCGCG
 1021 CGGAACACCG GCCCGGGCAA GGGCCCTGAA GCGGCCAGGT TCGTCTGGAT TGTTGACCAA
 1081 ACGATTTAAC CCGCCAGGTG GCGTCCGCCC ATCCTCCTAA TCCAGTATGG GGGCCCGGGA
 1141 AACCACCCTT TAAATTCAAT TCCCAGAACC GGCCGCCCAA TGGCGGGCGG CCCCGAATTT
 1201 TGGGGTGGGA AATGGTGCCG CGAGAATTTT AACCCCACAG AGCCGGCGCC GAGAGAGGGT
1261 CGTTC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles