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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-EGFP-ID

Expression clone of GFP under the control of CAG promoter, HisD resistance.

Catalog number RDB08527
Resource name pPyCAG-EGFP-ID
Alternative name stock no 10504
Clone info. Expression clone of GFP under the control of CAG promoter, HisD resistance.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 6.7 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08527 pPyCAG-EGFP-ID DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB08527z.seq
            Remarks, protocol and/or map (gif) RDB08527a.gif

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08527_A4Fo.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Sequence file: RDB08527_A4Foa.seq check
            >08527_08527_A4Fo_pAxCA_F1_A05_02.ab1
                1 TGTCCAGCGT CTTCTCAGTT CATGCCTTCT TCTTTTTCTA CAGTTCTGGG CAACGTGCTG
               61 GTTATTGTGG GAAACAAGCA GGTTTGGCAA AGAATTCCTC GAGATCCATT GTGGTTCAGT
              121 GCGGCCGATC CCCGGGTACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC
              181 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG
              241 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
              301 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG
              361 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
              421 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC
              481 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC
              541 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC
              601 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC
              661 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC
              721 GACGGCCCCG TGCTGCTGCT CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA
              781 AGACCCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA AGTTCGTGAC CGCCGCCCGG
              841 GATCACTCTC GGCATTGGAC GAGCTGTTAC AAGTTAAAGG CGGCCGGGCC GCAACTGGGC
            901 CACAATGGGA TTCTC
            //
            Primer: IRES-F
            Sequence file: RDB08527_A4Fob.seq check
            >08527_08527_A4Fo_IRES-F_B05_05.ab1
                1 CTGCCCTCGG GCCTTTAATG GTGGTTTAGT CGAGGTTAAA AAACGTCTAC GCCCCCCGAA
               61 CCACGGGGAC GTGGTTTTCA AAAAGAAAAA CACGATGATA ATATGGCCAC AACCATGAGC
              121 TTCAATACCC TGATTGACTG GAACAGCTGT AGCCCTGAAC AGCAGCGTGC GCTGCTGACG
              181 CGTCCGGCGA TTTCCGCCTC TGACAGTATT ACCCGGACGG TCAGCGATAT TCTGGATAAT
              241 GTAAAAACGC GCGGTGACGA TGCCCTGCGT GAATACAGCG CTAAATTTGA TAAAACAGAA
              301 GTGACAGCGC TACGCGTCAC CCCTGAAGAG ATCGCCGCCG CCGGCGCGCG TCTGAGCGAC
              361 GAATTAAAAC AGGCGATGAC CGCTGCCGTC AAAAATATTG AAACGTTCCA TTCCACGCAG
              421 ACGCTACCGC CTGTAGATGT GGAAACCCAG CCAGGCGTGC GTTGCCAGCA GGTTACGCGT
              481 CCCGTCTCGT CTGTCGGTCT GTATATTCCC GGCGGCTCGG CTCCGCTCTT CTCAACGGTG
              541 CTGATGCTGG CGACGCCGGC GCGCATTGCG GGATGCCAGA AGGTGGTTCT GTGCTCGCCG
              601 CCGCCCATCG CTGATGAAAT CCTCTATGCG GCGCAACTGT GTGGCGTGCA GGAAATCTTT
              661 AACGTCGGCG GCGCGCAGGC GATTGCCGCT CTGGCCTTCG GCAGCGAGTC CGTACCGAAA
              721 GTGGATAAAA TTTTTGGCCC CGGCAACGCC TTTGTAACCG AAGCCAAACG TCAGGTCAGG
              781 CCAGCGTCTC GGACGGCGCG GCTATCGATA TGCCAGCCGG GCCGTTCTGA AGTACCTGGT
              841 GATCGCAGAC AGCCGGCGCA ACACCCGGAA TTTCGTTGCT TCTGACCTGC TCTCCCAAGG
              901 CTGAGCACGG GCCCGGGATT TCCCAGGTGA TTCCTGCTTG ACGCCCTGAT GCTGACCATT
              961 GCCCGCAAGG TGGGCGGGAG GGCGGGTAGA ACGTTCAACC TGGCGGAAAC TGCCGGCGCG
             1021 CGGAACACCG GCCCGGGCAA GGGCCCTGAA GCGGCCAGGT TCGTCTGGAT TGTTGACCAA
             1081 ACGATTTAAC CCGCCAGGTG GCGTCCGCCC ATCCTCCTAA TCCAGTATGG GGGCCCGGGA
             1141 AACCACCCTT TAAATTCAAT TCCCAGAACC GGCCGCCCAA TGGCGGGCGG CCCCGAATTT
             1201 TGGGGTGGGA AATGGTGCCG CGAGAATTTT AACCCCACAG AGCCGGCGCC GAGAGAGGGT
            1261 CGTTC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl