Expression clone of GFP under the control of CAG promoter, HisD resistance.
| Catalog number | RDB08527 |
|---|---|
| Resource name | pPyCAG-EGFP-ID |
| Alternative name | stock no 10504 |
| Clone info. | Expression clone of GFP under the control of CAG promoter, HisD resistance. |
| Vector backbone | pPyCAG (Plasmid) |
| Size of vector backbone | 6.7 kb |
| Selectable markers | Amp^r |
| Gene/insert name | Aequorea victoria GFP cDNA |
| Depositor | Niwa, Hitoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | ((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08527 | pPyCAG-EGFP-ID | DNA solution |
Electronic file
| Electronic file | Sequence RDB08527z.seq from Depositor |
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| Electronic file | Map RDB08527a.gif from Depositor
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References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08527_A4Fo.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxCA_F1 | RDB08527_A4Foa.seq |
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>08527_08527_A4Fo_pAxCA_F1_A05_02.ab1 1 TGTCCAGCGT CTTCTCAGTT CATGCCTTCT TCTTTTTCTA CAGTTCTGGG CAACGTGCTG 61 GTTATTGTGG GAAACAAGCA GGTTTGGCAA AGAATTCCTC GAGATCCATT GTGGTTCAGT 121 GCGGCCGATC CCCGGGTACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC 181 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG 241 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC 301 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG 361 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC 421 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC 481 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC 541 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC 601 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC 661 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC 721 GACGGCCCCG TGCTGCTGCT CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA 781 AGACCCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA AGTTCGTGAC CGCCGCCCGG 841 GATCACTCTC GGCATTGGAC GAGCTGTTAC AAGTTAAAGG CGGCCGGGCC GCAACTGGGC 901 CACAATGGGA TTCTC // |
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| Primer: IRES-F | RDB08527_A4Fob.seq |
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>08527_08527_A4Fo_IRES-F_B05_05.ab1 1 CTGCCCTCGG GCCTTTAATG GTGGTTTAGT CGAGGTTAAA AAACGTCTAC GCCCCCCGAA 61 CCACGGGGAC GTGGTTTTCA AAAAGAAAAA CACGATGATA ATATGGCCAC AACCATGAGC 121 TTCAATACCC TGATTGACTG GAACAGCTGT AGCCCTGAAC AGCAGCGTGC GCTGCTGACG 181 CGTCCGGCGA TTTCCGCCTC TGACAGTATT ACCCGGACGG TCAGCGATAT TCTGGATAAT 241 GTAAAAACGC GCGGTGACGA TGCCCTGCGT GAATACAGCG CTAAATTTGA TAAAACAGAA 301 GTGACAGCGC TACGCGTCAC CCCTGAAGAG ATCGCCGCCG CCGGCGCGCG TCTGAGCGAC 361 GAATTAAAAC AGGCGATGAC CGCTGCCGTC AAAAATATTG AAACGTTCCA TTCCACGCAG 421 ACGCTACCGC CTGTAGATGT GGAAACCCAG CCAGGCGTGC GTTGCCAGCA GGTTACGCGT 481 CCCGTCTCGT CTGTCGGTCT GTATATTCCC GGCGGCTCGG CTCCGCTCTT CTCAACGGTG 541 CTGATGCTGG CGACGCCGGC GCGCATTGCG GGATGCCAGA AGGTGGTTCT GTGCTCGCCG 601 CCGCCCATCG CTGATGAAAT CCTCTATGCG GCGCAACTGT GTGGCGTGCA GGAAATCTTT 661 AACGTCGGCG GCGCGCAGGC GATTGCCGCT CTGGCCTTCG GCAGCGAGTC CGTACCGAAA 721 GTGGATAAAA TTTTTGGCCC CGGCAACGCC TTTGTAACCG AAGCCAAACG TCAGGTCAGG 781 CCAGCGTCTC GGACGGCGCG GCTATCGATA TGCCAGCCGG GCCGTTCTGA AGTACCTGGT 841 GATCGCAGAC AGCCGGCGCA ACACCCGGAA TTTCGTTGCT TCTGACCTGC TCTCCCAAGG 901 CTGAGCACGG GCCCGGGATT TCCCAGGTGA TTCCTGCTTG ACGCCCTGAT GCTGACCATT 961 GCCCGCAAGG TGGGCGGGAG GGCGGGTAGA ACGTTCAACC TGGCGGAAAC TGCCGGCGCG 1021 CGGAACACCG GCCCGGGCAA GGGCCCTGAA GCGGCCAGGT TCGTCTGGAT TGTTGACCAA 1081 ACGATTTAAC CCGCCAGGTG GCGTCCGCCC ATCCTCCTAA TCCAGTATGG GGGCCCGGGA 1141 AACCACCCTT TAAATTCAAT TCCCAGAACC GGCCGCCCAA TGGCGGGCGG CCCCGAATTT 1201 TGGGGTGGGA AATGGTGCCG CGAGAATTTT AACCCCACAG AGCCGGCGCC GAGAGAGGGT 1261 CGTTC // |
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Sequencing and PCR primers for primer information.
References
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl