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pPyCAG-EGFP-ID

Expression clone of GFP under the control of CAG promoter, HisD resistance.

Catalog number RDB08527
Resource name pPyCAG-EGFP-ID
Alternative name stock no 10504
Clone info. Expression clone of GFP under the control of CAG promoter, HisD resistance.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 6.7 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested().
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Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08527 pPyCAG-EGFP-ID DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08527z.seq from Depositor
Electronic file Map RDB08527a.gif from Depositor

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References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08527_A4Fo.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB08527_A4Foa.seq
>08527_08527_A4Fo_pAxCA_F1_A05_02.ab1
    1 TGTCCAGCGT CTTCTCAGTT CATGCCTTCT TCTTTTTCTA CAGTTCTGGG CAACGTGCTG
   61 GTTATTGTGG GAAACAAGCA GGTTTGGCAA AGAATTCCTC GAGATCCATT GTGGTTCAGT
  121 GCGGCCGATC CCCGGGTACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC
  181 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG
  241 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
  301 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG
  361 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
  421 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC
  481 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC
  541 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC
  601 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC
  661 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC
  721 GACGGCCCCG TGCTGCTGCT CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA
  781 AGACCCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA AGTTCGTGAC CGCCGCCCGG
  841 GATCACTCTC GGCATTGGAC GAGCTGTTAC AAGTTAAAGG CGGCCGGGCC GCAACTGGGC
901 CACAATGGGA TTCTC
//
Primer: IRES-F RDB08527_A4Fob.seq
>08527_08527_A4Fo_IRES-F_B05_05.ab1
    1 CTGCCCTCGG GCCTTTAATG GTGGTTTAGT CGAGGTTAAA AAACGTCTAC GCCCCCCGAA
   61 CCACGGGGAC GTGGTTTTCA AAAAGAAAAA CACGATGATA ATATGGCCAC AACCATGAGC
  121 TTCAATACCC TGATTGACTG GAACAGCTGT AGCCCTGAAC AGCAGCGTGC GCTGCTGACG
  181 CGTCCGGCGA TTTCCGCCTC TGACAGTATT ACCCGGACGG TCAGCGATAT TCTGGATAAT
  241 GTAAAAACGC GCGGTGACGA TGCCCTGCGT GAATACAGCG CTAAATTTGA TAAAACAGAA
  301 GTGACAGCGC TACGCGTCAC CCCTGAAGAG ATCGCCGCCG CCGGCGCGCG TCTGAGCGAC
  361 GAATTAAAAC AGGCGATGAC CGCTGCCGTC AAAAATATTG AAACGTTCCA TTCCACGCAG
  421 ACGCTACCGC CTGTAGATGT GGAAACCCAG CCAGGCGTGC GTTGCCAGCA GGTTACGCGT
  481 CCCGTCTCGT CTGTCGGTCT GTATATTCCC GGCGGCTCGG CTCCGCTCTT CTCAACGGTG
  541 CTGATGCTGG CGACGCCGGC GCGCATTGCG GGATGCCAGA AGGTGGTTCT GTGCTCGCCG
  601 CCGCCCATCG CTGATGAAAT CCTCTATGCG GCGCAACTGT GTGGCGTGCA GGAAATCTTT
  661 AACGTCGGCG GCGCGCAGGC GATTGCCGCT CTGGCCTTCG GCAGCGAGTC CGTACCGAAA
  721 GTGGATAAAA TTTTTGGCCC CGGCAACGCC TTTGTAACCG AAGCCAAACG TCAGGTCAGG
  781 CCAGCGTCTC GGACGGCGCG GCTATCGATA TGCCAGCCGG GCCGTTCTGA AGTACCTGGT
  841 GATCGCAGAC AGCCGGCGCA ACACCCGGAA TTTCGTTGCT TCTGACCTGC TCTCCCAAGG
  901 CTGAGCACGG GCCCGGGATT TCCCAGGTGA TTCCTGCTTG ACGCCCTGAT GCTGACCATT
  961 GCCCGCAAGG TGGGCGGGAG GGCGGGTAGA ACGTTCAACC TGGCGGAAAC TGCCGGCGCG
 1021 CGGAACACCG GCCCGGGCAA GGGCCCTGAA GCGGCCAGGT TCGTCTGGAT TGTTGACCAA
 1081 ACGATTTAAC CCGCCAGGTG GCGTCCGCCC ATCCTCCTAA TCCAGTATGG GGGCCCGGGA
 1141 AACCACCCTT TAAATTCAAT TCCCAGAACC GGCCGCCCAA TGGCGGGCGG CCCCGAATTT
 1201 TGGGGTGGGA AATGGTGCCG CGAGAATTTT AACCCCACAG AGCCGGCGCC GAGAGAGGGT
1261 CGTTC
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.11

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