Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-EGFP-ID

Expression clone of GFP under the control of CAG promoter, HisD resistance.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence	RDB08527z.seq
Remarks, protocol and/or map (gif)	RDB08527a.gif

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08527_A4Fo.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB08527_A4Foa.seq
>08527_08527_A4Fo_pAxCA_F1_A05_02.ab1     1 TGTCCAGCGT CTTCTCAGTT CATGCCTTCT TCTTTTTCTA CAGTTCTGGG CAACGTGCTG    61 GTTATTGTGG GAAACAAGCA GGTTTGGCAA AGAATTCCTC GAGATCCATT GTGGTTCAGT   121 GCGGCCGATC CCCGGGTACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC   181 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG   241 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC   301 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG   361 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC   421 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC   481 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC   541 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC   601 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC   661 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC   721 GACGGCCCCG TGCTGCTGCT CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA   781 AGACCCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA AGTTCGTGAC CGCCGCCCGG   841 GATCACTCTC GGCATTGGAC GAGCTGTTAC AAGTTAAAGG CGGCCGGGCC GCAACTGGGC 901 CACAATGGGA TTCTC //
Primer: IRES-F Sequence file: RDB08527_A4Fob.seq
>08527_08527_A4Fo_IRES-F_B05_05.ab1     1 CTGCCCTCGG GCCTTTAATG GTGGTTTAGT CGAGGTTAAA AAACGTCTAC GCCCCCCGAA    61 CCACGGGGAC GTGGTTTTCA AAAAGAAAAA CACGATGATA ATATGGCCAC AACCATGAGC   121 TTCAATACCC TGATTGACTG GAACAGCTGT AGCCCTGAAC AGCAGCGTGC GCTGCTGACG   181 CGTCCGGCGA TTTCCGCCTC TGACAGTATT ACCCGGACGG TCAGCGATAT TCTGGATAAT   241 GTAAAAACGC GCGGTGACGA TGCCCTGCGT GAATACAGCG CTAAATTTGA TAAAACAGAA   301 GTGACAGCGC TACGCGTCAC CCCTGAAGAG ATCGCCGCCG CCGGCGCGCG TCTGAGCGAC   361 GAATTAAAAC AGGCGATGAC CGCTGCCGTC AAAAATATTG AAACGTTCCA TTCCACGCAG   421 ACGCTACCGC CTGTAGATGT GGAAACCCAG CCAGGCGTGC GTTGCCAGCA GGTTACGCGT   481 CCCGTCTCGT CTGTCGGTCT GTATATTCCC GGCGGCTCGG CTCCGCTCTT CTCAACGGTG   541 CTGATGCTGG CGACGCCGGC GCGCATTGCG GGATGCCAGA AGGTGGTTCT GTGCTCGCCG   601 CCGCCCATCG CTGATGAAAT CCTCTATGCG GCGCAACTGT GTGGCGTGCA GGAAATCTTT   661 AACGTCGGCG GCGCGCAGGC GATTGCCGCT CTGGCCTTCG GCAGCGAGTC CGTACCGAAA   721 GTGGATAAAA TTTTTGGCCC CGGCAACGCC TTTGTAACCG AAGCCAAACG TCAGGTCAGG   781 CCAGCGTCTC GGACGGCGCG GCTATCGATA TGCCAGCCGG GCCGTTCTGA AGTACCTGGT   841 GATCGCAGAC AGCCGGCGCA ACACCCGGAA TTTCGTTGCT TCTGACCTGC TCTCCCAAGG   901 CTGAGCACGG GCCCGGGATT TCCCAGGTGA TTCCTGCTTG ACGCCCTGAT GCTGACCATT   961 GCCCGCAAGG TGGGCGGGAG GGCGGGTAGA ACGTTCAACC TGGCGGAAAC TGCCGGCGCG  1021 CGGAACACCG GCCCGGGCAA GGGCCCTGAA GCGGCCAGGT TCGTCTGGAT TGTTGACCAA  1081 ACGATTTAAC CCGCCAGGTG GCGTCCGCCC ATCCTCCTAA TCCAGTATGG GGGCCCGGGA  1141 AACCACCCTT TAAATTCAAT TCCCAGAACC GGCCGCCCAA TGGCGGGCGG CCCCGAATTT  1201 TGGGGTGGGA AATGGTGCCG CGAGAATTTT AACCCCACAG AGCCGGCGCC GAGAGAGGGT 1261 CGTTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl