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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-EGFP-IP

Expression clone of GFP under the control of CAG promoter, puromycin resistance.

Catalog number RDB08525
Resource name pPyCAG-EGFP-IP
Alternative name stock no 627
Clone info. Expression clone of GFP under the control of CAG promoter, puromycin resistance.
SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 6.6 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08525 pPyCAG-EGFP-IP DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB08525z.seq
Electronic file Map RDB08525a.gif

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08525_A3L4p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Region: EGFP 5'
Sequence file: RDB08525_A3L4c.seq check
>08525_08525_A3L4_pAxCA_F1_G05_20.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCCTT CTTCTTTTTC CNNCNGCTCC TGGGCAACGT
   61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC CTCGAGATCC ATTGTGGCCA
  121 GTGCGGCCGA TCCCCGGGTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA
  181 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG
  241 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA
  301 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC
  361 AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC
  421 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC
  481 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG
  541 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA
  601 ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC
  661 ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG
  721 GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN CACCCAGTCC GCCCTGAGCA
  781 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA NTTCGTGACC GCCGCCGGGN
  841 TCACTCTCGG CATGGNCGAN CTGTACAAGT AAAGCGGCCG GCCGCNCTGG NNNCAANGGN
  901 TCTCGANGAN NTNNNTCCNC NNNNGCNNGN TGCCNNTCAN NANNNGGNGG CNNNNGNNNC
961 CNNNNGGNNN NNNANNNNNN NNNN
//
Primer: IRES-R
Region: EGFP 3'
Sequence file: RDB08525_A3L4d.seq check
>08525_08525_B0F9F_IRES_R_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTTANGGG GGGGGGNNGN NNNNGGGGGG GGGGCGGAAT
   61 TCGATATCAA GCNNNNNNAG CGGCCGCTCG ATACCGTCGA TCTCAGTGGT ATTTGTGAGC
  121 CAGTTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG AGTGAATTCC
  181 TCGAGATCCA TTGTGGCCAG TGCGGCCGGC CGCTTTACTT GTACAGCTCG TCCATGCCGA
  241 GAGTGATCCC GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG
  301 GGTCTTTGCT CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC
  361 CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA
  421 TGTTGTGGCG GATCTTGAAG TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT
  481 AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT
  541 TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT
  601 CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC
  661 CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TANCGGCTGA
  721 AGCACTGCAC GCCGTANGTC ANGGNGGTCA CGANGGTGGG CCAGGGCACN GGCAGCTTGC
  781 CGGTGGTGCA GATGAANCTT CAGGGTCAGC TTGCCGTNNG NGGCATCNCC CTCNNCCNCN
  841 CNNGNANACG CTGNNCTTGT GGNCGTTTNN NNCNNCNNNC NGCNCGNNNN NNNNGGGNNC
901 CACCCCNGNN NANNNNCTNC NTCNNCNNTG CNNNANCNAN GGNNGNNNNN NNNNN
//
Primer: Amp_R
Region: polyoma ori,SV40 p(A),HCMVIEE
Sequence file: RDB08525_A3L4e.seq check
>08525_08525_B0F9F_3_Amp_R_H06_24_ABI24.ab1
    1 NNNNNNNNNN NNNNGCTTTG GNANNNNNAG ANTGTATTTC CTGGAAATTA ATGTTTATTC
   61 NATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG
  121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC
  181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC
  241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG
  301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG
  361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT
  421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT
  481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA
  541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG
  601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
  661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT
  721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT
  781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGNCCGCCTG
  841 GCTGANCGCC NNACGACCCC CGCCNATTGA CGTCAATNAT GACGTANGTN CCCNTANNAA
901 CGCCNATANG GNNTTTNCAT NNNNNNNNNN
//
Primer: IRES-F
Region: IRES, PuroR
Sequence file: RDB08525_A3L4f.seq check
>08525_08525_A3L4_IRES-F_H05_23.ab1
    1 NNNNNNNNNN NNNNTTNNGT CGANGNNNNN NAACGTCTAG GCCCCCCGAA CCNCGGGGNN
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
  121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
  181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
  241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
  301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCNCGCCG GANANCGTCG AANCGGGGGG
  361 CGGNGTTCGC CGANATCGGC CCGCGCNTGG NCGANTNNAN CNGNTTNCCG GNTNGNNCNC
421 NCNNNNANNN NNNNNNANNN CNNCNGGNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

reference Tran, T.H.Y., Live cell imaging of X chromosome reactivation during somatic cell reprogramming. Biochem. Biophys. Rep. 15: 86-92 (2018). PMID 30094351.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl