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pPyCAG-EGFP-IP

Expression clone of GFP under the control of CAG promoter, puromycin resistance.

Catalog number RDB08525
Resource name pPyCAG-EGFP-IP
Alternative name stock no 627
Clone info. Expression clone of GFP under the control of CAG promoter, puromycin resistance.
SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 6.6 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested().
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Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08525 pPyCAG-EGFP-IP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08525z.seq from Depositor
Electronic file Map RDB08525a.gif from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08525_A3L4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 | Sequence: RDB08525_A3L4a_v2.seq
>08525_08525_A3L4_pAxCA_F1_G05_20.ab1
    1 CTTAGTCGAC GTCTGCTCAT GTTATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT
   61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC CTCGAGATCC ATTGTGGCCA
  121 GTGCGGCCGA TCCCCGGGTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA
  181 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG
  241 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA
  301 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC
  361 AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC
  421 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC
  481 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG
  541 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA
  601 ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC
  661 ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG
  721 GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA
  781 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA
  841 TCACTCTCGG CATGGACGAG CTGTACAAGT AAAGCGGCCG GCCGCACTGG CCACAATGGA
  901 TCTCGAGGAA TTCACTCCTC AGGTGCAGGC TGCCTATCAG AAGGTGGTGG CTGGTGTGGC
  961 CAACTGGCTC ACAAATACCA CTGAGATCGA CGGTATCGAG CGGGCCGCTC GATAAGCTTT
 1021 GATATCGATT CCGGCCGCCC CCCGTCCTCC CTTCCCCCCC CCTTACGATA ACTGGACCGA
1081 AGTCGCATGA TTACGCCGGG TGTGCGATTT GTCCTAATAT GGTTA
//
Primer: IRES-F | Sequence: RDB08525_A3L4b_v2.seq
>08525_08525_A3L4_IRES-F_H05_23.ab1
    1 GCAGTCTTTA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
  121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
  181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
  241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
  301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGG
  361 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGGTTCCCG GCTGGGCCGC
  421 GCAGCAAACA GATGGAAAGC CTCCTGGCCG CCGCACCGGG CCCAAGGAGC CCGCCGTGGT
  481 TTCCTGGGCC ACCGTCGGCG TCTCGGCCCG ACCACCAGGG GCAAGGTTCT GGGGCACGCC
  541 CTCCTTGCTC CCCCGAATGT GAAGGCGGGC CAACCCCCCC GGGGTTGCCC CCCTTCTTGG
  601 AAAAACTCCC GGGCCCCCCA AACTCCCCTT TTTAAAAGGG GTTGGTTTTC CCGTCCCCCC
  661 CCAAATTAAG GGGGCCCAAA GAACCCCCCC CCTGGGTGGT TGAACCCCAA ACCCCGGGGG
  721 CCTGGAACCC CCCCCCCAAA CCCAAAGCCC CAACAAAAGG AACGCCCACC CCCCGCCCCC
  781 GTATATTTAA AACCCCCGTT TAACCCCCCC CGGGGCCTTT TTTTTTTCCC CCCCCTTTTT
  841 TTTTTTCCCT CCCCCCCCCC TTTTTTTACC CGGGAAGGGG GGGTTCCAGT TTTTTTTTTT
  901 AAAAAAAAAA AAAAAAAACC CCCCCTTTTT GAAAGGGGGG GTTTTTTTTT TTGGGGGGGG
  961 GGGGGGGGGC ACCCCACGGG GGGGGGAGGA GAAAAAAAAA AGCTCTCGGG GGGGGGGGGG
 1021 GGTTTTTTTT TCTTTTGGGG GAAAAAAAAA AAACCCCCCC CCCCCCGCGC GGGAAAAGAG
 1081 GGAAAAAGCT TTTTTTGTTG TTGGTGAGAT TATATTATTC CCTACATCCA ACCCCCACAA
 1141 ACAGACACAG ACGAGACAGA AAATAAGAGA CGGCGGCGCG GCGTCAGGTA GGATGATAAC
 1201 ACACACTACC ATACTGATTG TTGTCGTCGG CCGTCTGGTC GTCTGCTCGT TCGATGTAGC
1261 GAC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

reference Tran, T.H.Y., Live cell imaging of X chromosome reactivation during somatic cell reprogramming. Biochem. Biophys. Rep. 15: 86-92 (2018). PMID 30094351.

2019.11.02

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