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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-EGFP-IP

Expression clone of GFP under the control of CAG promoter, puromycin resistance.

Catalog number RDB08525
Resource name pPyCAG-EGFP-IP
Alternative name stock no 627
Clone info. Expression clone of GFP under the control of CAG promoter, puromycin resistance.
Comment SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 6.6 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08525 pPyCAG-EGFP-IP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB08525z.seq
            Remarks, protocol and/or map (gif) RDB08525a.gif

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08525_A3L4p1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Region: EGFP 5'
            Sequence file: RDB08525_A3L4c.seq check
            >08525_08525_A3L4_pAxCA_F1_G05_20.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNCCTT CTTCTTTTTC CNNCNGCTCC TGGGCAACGT
               61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC CTCGAGATCC ATTGTGGCCA
              121 GTGCGGCCGA TCCCCGGGTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA
              181 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG
              241 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA
              301 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC
              361 AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC
              421 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC
              481 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG
              541 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA
              601 ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC
              661 ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG
              721 GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN CACCCAGTCC GCCCTGAGCA
              781 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA NTTCGTGACC GCCGCCGGGN
              841 TCACTCTCGG CATGGNCGAN CTGTACAAGT AAAGCGGCCG GCCGCNCTGG NNNCAANGGN
              901 TCTCGANGAN NTNNNTCCNC NNNNGCNNGN TGCCNNTCAN NANNNGGNGG CNNNNGNNNC
            961 CNNNNGGNNN NNNANNNNNN NNNN
            //
            Primer: IRES-R
            Region: EGFP 3'
            Sequence file: RDB08525_A3L4d.seq check
            >08525_08525_B0F9F_IRES_R_B02_05_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNTTANGGG GGGGGGNNGN NNNNGGGGGG GGGGCGGAAT
               61 TCGATATCAA GCNNNNNNAG CGGCCGCTCG ATACCGTCGA TCTCAGTGGT ATTTGTGAGC
              121 CAGTTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG AGTGAATTCC
              181 TCGAGATCCA TTGTGGCCAG TGCGGCCGGC CGCTTTACTT GTACAGCTCG TCCATGCCGA
              241 GAGTGATCCC GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG
              301 GGTCTTTGCT CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC
              361 CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA
              421 TGTTGTGGCG GATCTTGAAG TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT
              481 AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT
              541 TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT
              601 CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC
              661 CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TANCGGCTGA
              721 AGCACTGCAC GCCGTANGTC ANGGNGGTCA CGANGGTGGG CCAGGGCACN GGCAGCTTGC
              781 CGGTGGTGCA GATGAANCTT CAGGGTCAGC TTGCCGTNNG NGGCATCNCC CTCNNCCNCN
              841 CNNGNANACG CTGNNCTTGT GGNCGTTTNN NNCNNCNNNC NGCNCGNNNN NNNNGGGNNC
            901 CACCCCNGNN NANNNNCTNC NTCNNCNNTG CNNNANCNAN GGNNGNNNNN NNNNN
            //
            Primer: Amp_R
            Region: polyoma ori,SV40 p(A),HCMVIEE
            Sequence file: RDB08525_A3L4e.seq check
            >08525_08525_B0F9F_3_Amp_R_H06_24_ABI24.ab1
                1 NNNNNNNNNN NNNNGCTTTG GNANNNNNAG ANTGTATTTC CTGGAAATTA ATGTTTATTC
               61 NATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG
              121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC
              181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC
              241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG
              301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG
              361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT
              421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT
              481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA
              541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG
              601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
              661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT
              721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT
              781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGNCCGCCTG
              841 GCTGANCGCC NNACGACCCC CGCCNATTGA CGTCAATNAT GACGTANGTN CCCNTANNAA
            901 CGCCNATANG GNNTTTNCAT NNNNNNNNNN
            //
            Primer: IRES-F
            Region: IRES, PuroR
            Sequence file: RDB08525_A3L4f.seq check
            >08525_08525_A3L4_IRES-F_H05_23.ab1
                1 NNNNNNNNNN NNNNTTNNGT CGANGNNNNN NAACGTCTAG GCCCCCCGAA CCNCGGGGNN
               61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
              121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
              181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
              241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
              301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCNCGCCG GANANCGTCG AANCGGGGGG
              361 CGGNGTTCGC CGANATCGGC CCGCGCNTGG NCGANTNNAN CNGNTTNCCG GNTNGNNCNC
            421 NCNNNNANNN NNNNNNANNN CNNCNGGNNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            reference Tran, T.H.Y., Live cell imaging of X chromosome reactivation during somatic cell reprogramming. Biochem. Biophys. Rep. 15: 86-92 (2018). PMID 30094351.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl