Expression clone of GFP under the control of CAG promoter, puromycin resistance.
| Catalog number | RDB08525 |
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| Resource name | pPyCAG-EGFP-IP |
| Alternative name | stock no 627 |
| Clone info. | Expression clone of GFP under the control of CAG promoter, puromycin resistance. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20. |
| Vector backbone | pPyCAG (Plasmid) |
| Size of vector backbone | 6.6 kb |
| Selectable markers | Amp^r |
| Gene/insert name | Aequorea victoria GFP cDNA |
| Depositor | Niwa, Hitoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08525 | pPyCAG-EGFP-IP | DNA solution |
Electronic file
| Electronic file | Sequence RDB08525z.seq from Depositor |
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| Electronic file | Map RDB08525a.gif from Depositor
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Empty Backbone (English text) |
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| Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08525_A3L4p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxCA_F1 | Sequence: RDB08525_A3L4a_v2.seq |
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>08525_08525_A3L4_pAxCA_F1_G05_20.ab1 1 CTTAGTCGAC GTCTGCTCAT GTTATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT 61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC CTCGAGATCC ATTGTGGCCA 121 GTGCGGCCGA TCCCCGGGTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA 181 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG 241 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA 301 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC 361 AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC 421 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC 481 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG 541 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA 601 ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC 661 ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG 721 GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA 781 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA 841 TCACTCTCGG CATGGACGAG CTGTACAAGT AAAGCGGCCG GCCGCACTGG CCACAATGGA 901 TCTCGAGGAA TTCACTCCTC AGGTGCAGGC TGCCTATCAG AAGGTGGTGG CTGGTGTGGC 961 CAACTGGCTC ACAAATACCA CTGAGATCGA CGGTATCGAG CGGGCCGCTC GATAAGCTTT 1021 GATATCGATT CCGGCCGCCC CCCGTCCTCC CTTCCCCCCC CCTTACGATA ACTGGACCGA 1081 AGTCGCATGA TTACGCCGGG TGTGCGATTT GTCCTAATAT GGTTA // |
| Primer: IRES-F | Sequence: RDB08525_A3L4b_v2.seq |
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>08525_08525_A3L4_IRES-F_H05_23.ab1 1 GCAGTCTTTA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC 61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC 121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC 181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT 241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT 301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGG 361 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGGTTCCCG GCTGGGCCGC 421 GCAGCAAACA GATGGAAAGC CTCCTGGCCG CCGCACCGGG CCCAAGGAGC CCGCCGTGGT 481 TTCCTGGGCC ACCGTCGGCG TCTCGGCCCG ACCACCAGGG GCAAGGTTCT GGGGCACGCC 541 CTCCTTGCTC CCCCGAATGT GAAGGCGGGC CAACCCCCCC GGGGTTGCCC CCCTTCTTGG 601 AAAAACTCCC GGGCCCCCCA AACTCCCCTT TTTAAAAGGG GTTGGTTTTC CCGTCCCCCC 661 CCAAATTAAG GGGGCCCAAA GAACCCCCCC CCTGGGTGGT TGAACCCCAA ACCCCGGGGG 721 CCTGGAACCC CCCCCCCAAA CCCAAAGCCC CAACAAAAGG AACGCCCACC CCCCGCCCCC 781 GTATATTTAA AACCCCCGTT TAACCCCCCC CGGGGCCTTT TTTTTTTCCC CCCCCTTTTT 841 TTTTTTCCCT CCCCCCCCCC TTTTTTTACC CGGGAAGGGG GGGTTCCAGT TTTTTTTTTT 901 AAAAAAAAAA AAAAAAAACC CCCCCTTTTT GAAAGGGGGG GTTTTTTTTT TTGGGGGGGG 961 GGGGGGGGGC ACCCCACGGG GGGGGGAGGA GAAAAAAAAA AGCTCTCGGG GGGGGGGGGG 1021 GGTTTTTTTT TCTTTTGGGG GAAAAAAAAA AAACCCCCCC CCCCCCGCGC GGGAAAAGAG 1081 GGAAAAAGCT TTTTTTGTTG TTGGTGAGAT TATATTATTC CCTACATCCA ACCCCCACAA 1141 ACAGACACAG ACGAGACAGA AAATAAGAGA CGGCGGCGCG GCGTCAGGTA GGATGATAAC 1201 ACACACTACC ATACTGATTG TTGTCGTCGG CCGTCTGGTC GTCTGCTCGT TCGATGTAGC 1261 GAC // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| reference | Tran, T.H.Y., Live cell imaging of X chromosome reactivation during somatic cell reprogramming. Biochem. Biophys. Rep. 15: 86-92 (2018). PMID 30094351. |
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2019.11.02
GNP_filter3_RDBDEP_html_190927.pl