Expression clone of GFP under the control of CAG promoter, puromycin resistance.
Catalog number | RDB08525 |
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Resource name | pPyCAG-EGFP-IP |
Alternative name | stock no 627 |
Clone info. | Expression clone of GFP under the control of CAG promoter, puromycin resistance. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20. |
Vector backbone | pPyCAG (Plasmid) |
Size of vector backbone | 6.6 kb |
Selectable markers | Amp^r |
Gene/insert name | Aequorea victoria GFP cDNA |
Depositor|Developer | Niwa, Hitoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. |
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Additional terms and conditions for distribution | Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested. |
Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA) [Word ![]() Schedule A (GFP-A) [Link] |
Remarks | Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB08525 | pPyCAG-EGFP-IP | DNA solution |
Electronic file
Electronic file | Sequence RDB08525z.seq |
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Electronic file | Map RDB08525a.gif
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Empty Backbone (English text) |
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Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB08525_A3L4p1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pAxCA_F1 Region: EGFP 5' Sequence file: RDB08525_A3L4c.seq ![]() |
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>08525_08525_A3L4_pAxCA_F1_G05_20.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNCCTT CTTCTTTTTC CNNCNGCTCC TGGGCAACGT 61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC CTCGAGATCC ATTGTGGCCA 121 GTGCGGCCGA TCCCCGGGTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA 181 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG 241 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA 301 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC 361 AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC 421 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC 481 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG 541 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA 601 ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC 661 ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG 721 GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN CACCCAGTCC GCCCTGAGCA 781 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA NTTCGTGACC GCCGCCGGGN 841 TCACTCTCGG CATGGNCGAN CTGTACAAGT AAAGCGGCCG GCCGCNCTGG NNNCAANGGN 901 TCTCGANGAN NTNNNTCCNC NNNNGCNNGN TGCCNNTCAN NANNNGGNGG CNNNNGNNNC 961 CNNNNGGNNN NNNANNNNNN NNNN // |
Primer: IRES-R Region: EGFP 3' Sequence file: RDB08525_A3L4d.seq ![]() |
>08525_08525_B0F9F_IRES_R_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTTANGGG GGGGGGNNGN NNNNGGGGGG GGGGCGGAAT 61 TCGATATCAA GCNNNNNNAG CGGCCGCTCG ATACCGTCGA TCTCAGTGGT ATTTGTGAGC 121 CAGTTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG AGTGAATTCC 181 TCGAGATCCA TTGTGGCCAG TGCGGCCGGC CGCTTTACTT GTACAGCTCG TCCATGCCGA 241 GAGTGATCCC GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG 301 GGTCTTTGCT CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC 361 CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA 421 TGTTGTGGCG GATCTTGAAG TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT 481 AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT 541 TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT 601 CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC 661 CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TANCGGCTGA 721 AGCACTGCAC GCCGTANGTC ANGGNGGTCA CGANGGTGGG CCAGGGCACN GGCAGCTTGC 781 CGGTGGTGCA GATGAANCTT CAGGGTCAGC TTGCCGTNNG NGGCATCNCC CTCNNCCNCN 841 CNNGNANACG CTGNNCTTGT GGNCGTTTNN NNCNNCNNNC NGCNCGNNNN NNNNGGGNNC 901 CACCCCNGNN NANNNNCTNC NTCNNCNNTG CNNNANCNAN GGNNGNNNNN NNNNN // |
Primer: Amp_R Region: polyoma ori,SV40 p(A),HCMVIEE Sequence file: RDB08525_A3L4e.seq ![]() |
>08525_08525_B0F9F_3_Amp_R_H06_24_ABI24.ab1 1 NNNNNNNNNN NNNNGCTTTG GNANNNNNAG ANTGTATTTC CTGGAAATTA ATGTTTATTC 61 NATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG 121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC 181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC 241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG 301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG 361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT 421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT 481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA 541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG 601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT 721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT 781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGNCCGCCTG 841 GCTGANCGCC NNACGACCCC CGCCNATTGA CGTCAATNAT GACGTANGTN CCCNTANNAA 901 CGCCNATANG GNNTTTNCAT NNNNNNNNNN // |
Primer: IRES-F Region: IRES, PuroR Sequence file: RDB08525_A3L4f.seq ![]() |
>08525_08525_A3L4_IRES-F_H05_23.ab1 1 NNNNNNNNNN NNNNTTNNGT CGANGNNNNN NAACGTCTAG GCCCCCCGAA CCNCGGGGNN 61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC 121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC 181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT 241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT 301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCNCGCCG GANANCGTCG AANCGGGGGG 361 CGGNGTTCGC CGANATCGGC CCGCGCNTGG NCGANTNNAN CNGNTTNCCG GNTNGNNCNC 421 NCNNNNANNN NNNNNNANNN CNNCNGGNNN NNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
reference | Tran, T.H.Y., Live cell imaging of X chromosome reactivation during somatic cell reprogramming. Biochem. Biophys. Rep. 15: 86-92 (2018). PMID 30094351. |
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2020.10.02
GNP_filter3_RDBDEP_html_200316.pl