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p3xFLAG-CMV-14-hSVBP (CCDC23) (#RDB08520)

Expression construct of human SVBP (CCDC23) cDNA

Alternative name human small vasohibin binding protein
Clone info. Expression construct of human SVBP (CCDC23) cDNA. FLAG epitope tag sequence is translated with SVBP.
Vector backbone p3xFLAG-CMV-14 (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r
Gene/insert name Human small vasohibin binding protein cDNA
Depositor|Developer Sato, Yasufumi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Suzuki, Y. et al., J. Cell Sci. 123, 3094-4101, 2010.).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Suzuki, Y. et al., J. Cell Sci. 123, 3094-4101, 2010.)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08520 p3xFLAG-CMV-14-hSVBP (CCDC23) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3xFLAG-CMV-14-hSVBP (CCDC23) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08520).

Reference section:

Suzuki, Y., Kobayashi, M., Miyashita, H., Ohta, H., Sonoda, H., Sato, Y., Isolation of a small vasohibin-binding protein (SVBP) and its role in vasohibin secretion. J. Cell. Sci. 123, 3094-4101, (2010). PMID 20736312. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08520_A8Atp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB08520_A8Ata.seq check
>08520_08520_A8At_2_CMV-Forward_B02_05_ABI24.ab1
    1 CTAAATTACC CGCCCGTTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTAAGCTTGC GGCCGCCACC ATGGATCCAC
  121 CTGCACGTAA AGAAAAAACC AAAGTTAAAG AATCTGTCAG CAGAGTTGAG AAGGCCAAAC
  181 AGAAATCAGC CCAGCAGGAG CTGAAGCAGA GACAAAGAGC AGAGATCTAT GCCCTCAACA
  241 GAGTCATGAC AGAACTGGAG CAGCAGCAGT TTGATGAGTT CTGTAAACAG ATGCAGCCTC
  301 CTGGAGAATC TAGAGGATCC CGGGCTGACT ACAAAGACCA TGACGGTGAT TATAAAGATC
  361 ATGACATCGA CTACAAGGAT GACGATGACA AGTAGTGATC CCGGGTGGCA TCCCTGTGAC
  421 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC
  481 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG
  541 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG
  601 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC
  661 TCCTGGGGGT TCAAGCGATT CTCCTGCCTC AGCCCTCCCC GAGTTGGTTG GGATTCCAGG
  721 CATGCATGAC CCAGGCTCAG CTTAATTTTT GTTTTTTTTT GGTAGAGACG GGGTTTCACT
  781 CATAATTTGG CCAGGCTGGT CCCCAACTCC CAAATCTCAG GGGATCTACC ACCTTGGCCC
  841 TCCCAAATTG GGTGGGGATT AACAGGCGTT GAACCCCCTG GCTCCCCTTT CCCTGGTCCT
901 TCTGAATTTT AAAAATTA
//
Primer: SV40pro_F_V2
Sequence file: RDB08520_A8Atb.seq check
>08520_08520_A8At_2_hGHpolyA-R_C02_08_ABI24.ab1
    1 GCTAACCCCC CTCACCCCAT ATATTATAGA AGGACACCTA GTCAGACAAA ATGATGCAAC
   61 TTAATTTTAT TAGGACAAGG CTGGTGGGCA CTGGAGTGGC AACTTCCAGG GCCAGGAGAG
  121 GCACTGGGGA GGGGTCACAG GGATGCCACC CGGGATCACT ACTTGTCATC GTCATCCTTG
  181 TAGTCGATGT CATGATCTTT ATAATCACCG TCATGGTCTT TGTAGTCAGC CCGGGATCCT
  241 CTAGATTCTC CAGGAGGCTG CATCTGTTTA CAGAACTCAT CAAACTGCTG CTGCTCCAGT
  301 TCTGTCATGA CTCTGTTGAG GGCATAGATC TCTGCTCTTT GTCTCTGCTT CAGCTCCTGC
  361 TGGGCTGATT TCTGTTTGGC CTTCTCAACT CTGCTGACAG ATTCTTTAAC TTTGGTTTTT
  421 TCTTTACGTG CAGGTGGATC CATGGTGGCG GCCGCAAGCT TAATTCTGAC GGTTCACTAA
  481 ACGAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAACGG
  541 GGCGGGGTTA TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC
  601 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC
  661 ATTGGTGTAC TGCCAAAACC GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA
  721 CTGCCAAGTA GGAAAGTCCC GTAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT
  781 ACCGTCATTG ACGTCAATAG GGGGCGGACT TGGCATATGA TACACTTGAT GTACTGCCAA
  841 GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA TGGAAAGTCC CTATTGGCGT
  901 TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC
  961 CAGGCGGGCC ATTACCGGTA AGTTATGTAA CGGCGGAACT CCATATATGG GCTATTGAAC
 1021 TAATGACCCC CGTAATTGAA TTACTATTAA TAACCTAGTT CAATTATCAT GGTCAACATG
 1081 CGTTCATAGT GGACAATGGA GCAATATAAT GGTACTATTA TGATATGAGA ATTACTACAA
1141 CGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Suzuki, Y., Isolation of a small vasohibin-binding protein (SVBP) and its role in vasohibin secretion. J. Cell. Sci. 123, 3094-4101, (2010). PMID 20736312. [link to RRC of NBRP]