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pRS416-PADH1-gap1[K9R/K16R]yEGFP-TTEF1

Expression clone of S. cerevisiae GAP1 mutant [K9R/K16R] cDNA

Catalog number RDB08391
Resource name pRS416-PADH1-gap1[K9R/K16R]yEGFP-TTEF1
Clone info. Expression clone of GFP-tagged S. cerevisiae GAP1 mutant [K9R/K16R].
Vector backbone pRS416-PADH1-ccdB-yEGFP-TTEF1 (Plasmid)
Size of vector backbone 7.4 kb
Selectable markers Amp^r
Gene/insert name S. cerevisiae GAP1 cDNA
Depositor|Developer TAKAGI, Hiroshi |
 
Other clones in our bank S. cerevisiae GNP1 (KEGG orthology K16261) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Genes to cells, 18, 459-475 (2013)).
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB08391 pRS416-PADH1-gap1[K9R/K16R]yEGFP-TTEF1 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Sasaki, T., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Sasaki, T., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.

2021.03.25

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