RDB08386 - pRS416-PADH1-yEGFP-TTEF1

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pRS416-PADH1-yEGFP-TTEF1 (#RDB08386)

Empty expression vector backbone of S. cerevisiae

Clone info.	Empty expression vector backbone of S. cerevisiae to express GFP-tagged protein.
Vector backbone	pRS416-PADH1-yEGFP-TTEF1 (Plasmid)
Size of vector backbone	7.4 kb
Selectable markers	Amp^r
Depositor\|Developer	Takagi, Hiroshi \|

Sequence (full)	RDB08386zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Genes to cells, 18, 459-475 (2013)).

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Genes to cells, 18, 459-475 (2013)).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Genes to cells, 18, 459-475 (2013))。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08386	pRS416-PADH1-yEGFP-TTEF1	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pRS416-PADH1-yEGFP-TTEF1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08386).

Reference section:

Sasaki, T., Takagi, H., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08386_A2Bkp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: T7 pro, insert 3' (TEF ter,yeGFP 3') Sequence file: RDB08386_A2Bka.seq
>08386_08386_B0JjF_1_pUC-M13_-100_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNGGNANG CCNGGGTTTT CCCNGTCACG ACGTTGTAAA ACGACGGCCA 61 GTGNGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCATTAAG GGTTCTCGAG 121 TGCTCGTTTT CGACACTGGA TGGCGGCGTT AGTATCGAAT CGACAGCAGT ATAGCGACCA 181 GCATTCACAT ACGATTGACG CATGATATTA CTTTCTGCGC ACTTAACTTC GCATCTGGGC 241 AGATGATGTC GAGGCGAAAA AAAATATAAA TCACGCTAAC ATTTGATTAA AATAGAACAA 301 CTACAATATA AAAAAACTAT ACAAATGACA AGTTCTTGAA AACAAGAATC TTTTTATTGT 361 CAGTGCTGAT TAGGTCGACG CGGCCGCATA GGCCACTAGT GGATCTGATA TCATCGATGA 421 ATTCTCTGTC GGACCAGCAC CGGCACCGGC ACCAGCACCG GCACCAGCAC CTTTGTACAA 481 TTCATCCATA CCATGGGTAA TACCAGCAGC AGTAACAAAT TCTAACAAGA CCATGTGGTC 541 TCTCTTTTCG TTTGGATCTT TGGATAANGC AGANTGAGTG GATAAGTAAT GGTTGTCTGG 601 TAACAAGACT GGACCATCAC CAATTGGAGT ANTTTGTTGA TAATGNTCAG CTAATTGAAC 661 AGAACCATCT TCNNTGNTGT GTCTNATTTT NAANTTNANT TTGATANNNN TCNTTNGNTN 721 GNNNNNNNNN N //
Primer: ADHpro_C (Pr0003) Region: insert mid (ADH1 pro,yeGFP 5') Sequence file: RDB08386_A2Bkb.seq
>08386_08386_B0JjF_1_ADH1pro_F_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNTTT TGTTTCCTCG TCNTTGTTCT CGTTCCCTTT CTTCCTTGTT 61 TCTTTTTCTG CACAATATTT CAAGCTATAC CAAGCATACA ATCAACTCCA AGCTGGCCGC 121 TCTAGAACTA GTGGATCCCC CCCGGAATGT CTAAAGGTGA AGAATTATTC ACTGGTGTTG 181 TCCCAATTTT GGTTGAATTA GATGGTGATG TTAATGGTCA CAAATTTTCT GTCTCCGGTG 241 AAGGTGAAGG TGATGCTACT TACGGTAAAT TGACCTTAAA ATTTATTTGT ACTACTGGTA 301 AATTGCCAGT TCCATGGCCA ACCTTAGTCA CTACTTTCGG TTATGGTGTT CAATGTTTTG 361 CGAGATACCC AGATCATATG AAACAACATG ACTTTTTCAA GTCTGCCATG CCAGAAGGTT 421 ATGTTCAAGA AAGAACTATT TTTTTCAAAG ATGACGGTAA CTACAAGACC AGAGCTGAAG 481 TCAAGTTTGA AGGTGATACC TTAGTTAATA GAATCGAATT AAAAGGTATT GATTTTAAAG 541 AAGANGGTAA CATTTTAGGT CACAAATTGG AATACAACTA TAACTCTCAC AATGTTTACA 601 TCATGGCTGA CAAACAAAAG AATGGTATCA AAGTTAACTT CNAAATTAGA CACAACATTG 661 AAGANGGTTC TGTTCNNTTA NCTGANNATT ATCNANNAAA NANTCCNNTT NNNNNNGGNN 721 NNNNNNNN //
Primer: pBAC_lac_R (Pr0545) Region: insert 5' Sequence file: RDB08386_A2Bkc.seq
>08386_08386_B0JjF_1_pBAC_lac_R_A01_01_ABI08.ab1 1 NNNNNNNNNC NNNNNNAANG GGNNNAAAAG CTGGAGCTCG CCGGGNNCGA AGAAATGATG 61 GTAAATGAAA TAGGAAATCA AGGAGCATGA AGGCAAAAGA CAAATATAAG GGTCGAACGA 121 AAAATAAAGT GAAAAGTGTT GATATGATGT ATTTGGCTTT GCGGCGCCGA AAAAACGAGT 181 TTACGCAATT GCACAATCAT GCTGACTCTG TGGCGGACCC GCGCTCTTGC CGGCCCGGCG 241 ATAACGCTGG GCGTGAGGCT GTGCCCGGCG GAGTTTTTTG CGCCTGCATT TTCCAAGGTT 301 TACCCTGCGC TAAGGGGCGA GATTGGAGAA GCAATAAGAA TGCCGGTTGG GGTTGCGATG 361 ATGACGACCA CGACAACTGG TGTCATTATT TAAGTTGCCG AAAGAACCTG AGTGCATTTG 421 CAACATGAGT ATACTAGAAG AATGAGCCAA GACTTGCGAG ACGCGAGTTT GCCGGTGGTG 481 CGAACAATAG AGCGACCATG ACCTTGAAGG TGAGACGCGC ATAACCGCTA GAGTACTTTG 541 AAGAGGAAAC AGCAATAGGG TTGCTACCAG TATAAATAGA CAGGTACATA CAACACTGGA 601 AATGGTTGTC TGTTTGAGTA CGCTTTCAAT TCATTTGGGT GTGCACTTTA TTATGTTACA 661 ATATGGAANG GAACTTTACA CTTCTCCTAN GCNNNTATNN TAATTAANNN CNNNNGNTAG 721 TANNGNANNN GGGNNNNNNN CCNNNNNNNN NN //
Primer: yeGFP-M_R (Pr0898) Region: insert mid (yeGFP 3',TEF ter) Sequence file: RDB08386_A2Bkd.seq
>08386_08386_B0JjF_1_yeGFP-M_R_A05_02_ABI24.ab1 1 NNNNNNNNNN NNNCTNANTN NNNAGAATCG AATTAAAAGG TATTGATTTT AAAGAAGATG 61 GTAACATTTT AGGTCACAAA TTGGAATACA ACTATAACTC TCACAATGTT TACATCATGG 121 CTGACAAACA AAAGAATGGT ATCAAAGTTA ACTTCAAAAT TAGACACAAC ATTGAAGATG 181 GTTCTGTTCA ATTAGCTGAC CATTATCAAC AAAATACTCC AATTGGTGAT GGTCCAGTCT 241 TGTTACCAGA CAACCATTAC TTATCCACTC AATCTGCCTT ATCCAAAGAT CCAAACGAAA 301 AGAGAGACCA CATGGTCTTG TTAGAATTTG TTACTGCTGC TGGTATTACC CATGGTATGG 361 ATGAATTGTA CAAAGGTGCT GGTGCCGGTG CTGGTGCCGG TGCCGGTGCT GGTCCGACAG 421 AGAATTCATC GATGATATCA GATCCACTAG TGGCCTATGC GGCCGCGTCG ACCTAATCAG 481 CACTGACAAT AAAAAGATTC TTGTTTTCAA GAACTTGTCA TTTGTATAGT TTTTTTATAT 541 TGTAGTTGTT CTATTTTAAT CAAATGTTAG CGTGATTTAT ATTTTTTTTC GCCTCNACAT 601 CATCTGCCCA GATGCGAAGT TAAGTGCGCA NAAAGTAATA TCATGCGTNN ATCGNANGTG 661 AATGCTGGTC GCTATACTGC TGTCNNNTCN ATACTAACGN CNNNATNNNN NGNCNAANNN 721 NANCACTCNA NNNNCNNTNN NNNNNNNCNN NNNNNNNN //
Primer: URA3pro_F (Pr0170) Region: URA3 pro,URA3 Sequence file: RDB08386_A2Bke.seq
>08386_08386_B0JjF_1_URA3pro_F_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNTGNN NTTGCCNNGT ATTCTTAACC CAACTGCACA GAACAAAAAC 61 CTGCAGGAAA CGAAGATAAA TCATGTCGAA AGCTACATAT AAGGAACGTG CTGCTACTCA 121 TCCTAGTCCT GTTGCTGCCA AGCTATTTAA TATCATGCAC GAAAAGCAAA CAAACTTGTG 181 TGCTTCATTG GATGTTCGTA CCACCAAGGA ATTACTGGAG TTAGTTGAAG CATTAGGTCC 241 CAAAATTTGT TTACTAAAAA CACATGTGGA TATCTTGACT GATTTTTCCA TGGAGGGCAC 301 AGTTAAGCCG CTAAAGGCAT TATCCGCCAA GTACAATTTT TTACTCTTCG AAGACAGAAA 361 ATTTGCTGAC ATTGGTAATA CAGTCAAATT GCAGTACTCT GCGGGTGTAT ACAGAATAGC 421 AGAATGGGCA GACATTACGA ATGCACACGG TGTGGTGGGC CCAGGTATTG TTAGCGGTTT 481 GAAGCAGGCG GCAGAAGAAG TAACAAAGGA ACCTAGAGGC CTTTTGATGT TAGCAGAATT 541 GTCATGCAAG GGCTCCCTAT CTACTGGAGA ATATACTAAG GGTACTGTTG ACATTGCGAA 601 GAGCGACAAA GANTTTGTTA TCGGCTTTAT TGCTCAAAGA GACATGGGTG GAAGAGANGA 661 ANNNTACGAN TGGTTGANTA TGACACCNNG NGTGGGTTTA GANGANNNNG GNNACGCATT 721 GGNNCNNNNN NANNNNNNNN NN //
Primer: pGEX-RSEQ (Pr0077) Region: CEN_ARS Sequence file: RDB08386_A2Bkf.seq
>08386_08386_B0JjF_1_pGEX-RSEQ_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNA NNGGCCTCGT GNNACGCCTA TTTTTATAGG TTAATGTCAT 61 GATAATAATG GTTTCTTAGA CGGATCGCTT GCCTGTAACT TACACGCGCC TCGTATCTTT 121 TAATGATGGA ATAATTTGGG AATTTACTCT GTGTTTATTT ATTTTTATGT TTTGTATTTG 181 GATTTTAGAA AGTAAATAAA GAAGGTAGAA GAGTTACGGA ATGAAGAAAA AAAAATAAAC 241 AAAGGTTTAA AAAATTTCAA CAAAAAGCGT ACTTTACATA TATATTTATT AGACAAGAAA 301 AGCAGATTAA ATAGATATAC ATTCGATTAA CGATAAGTAA AATGTAAAAT CACAGGATTT 361 TCGTGTGTGG TCTTCTACAC AGACAAGATG AAACAATTCG GCATTAATAC CTGAGAGCAG 421 GAAGAGCAAG ATAAAAGGTA GTATTTGTTG GCGATCCCCC TAGAGTCTTT TACATCTTCG 481 GAAAACAAAA ACTATTTTTT CTTTAATTTC TTTTTTTACT TTCTATTTTT AATTTATATA 541 TTTATATTAA AAAATTTAAA TTATAATTAT TTTTATAGCA CGTGATGAAA AGGACCCAGG 601 TGGCACTTTT CGGGGNAATG TGCGCGGAAC CCCTATTTGT TTANTTTTCT AAATACATTC 661 NAATATGTAN CCGCTCATGA GACAATAACC NNGATAAATG CTTNNNNNNN ANTGNAAANN 721 NNNNNTATGN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sasaki, T., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290. [link to RRC of NBRP]

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