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pRS416-PADH1-yEGFP-TTEF1

Empty expression vector backbone of S. cerevisiae

Catalog number RDB08386
Resource name pRS416-PADH1-yEGFP-TTEF1
Clone info. Empty expression vector backbone of S. cerevisiae to express GFP-tagged protein.
Vector backbone pRS416-PADH1-yEGFP-TTEF1 (Plasmid)
Size of vector backbone 7.4 kb
Selectable markers Amp^r
Depositor|Developer TAKAGI, Hiroshi |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB08386zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Genes to cells, 18, 459-475 (2013)).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Genes to cells, 18, 459-475 (2013))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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            RDB08386 pRS416-PADH1-yEGFP-TTEF1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Sasaki, T., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08386_A2Bkp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-M13_-100 (Pr0310)
            Region: T7 pro, insert 3' (TEF ter,yeGFP 3')
            Sequence file: RDB08386_A2Bka.seq check
            >08386_08386_B0JjF_1_pUC-M13_-100_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNGGNANG CCNGGGTTTT CCCNGTCACG ACGTTGTAAA ACGACGGCCA
               61 GTGNGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGG TACCATTAAG GGTTCTCGAG
              121 TGCTCGTTTT CGACACTGGA TGGCGGCGTT AGTATCGAAT CGACAGCAGT ATAGCGACCA
              181 GCATTCACAT ACGATTGACG CATGATATTA CTTTCTGCGC ACTTAACTTC GCATCTGGGC
              241 AGATGATGTC GAGGCGAAAA AAAATATAAA TCACGCTAAC ATTTGATTAA AATAGAACAA
              301 CTACAATATA AAAAAACTAT ACAAATGACA AGTTCTTGAA AACAAGAATC TTTTTATTGT
              361 CAGTGCTGAT TAGGTCGACG CGGCCGCATA GGCCACTAGT GGATCTGATA TCATCGATGA
              421 ATTCTCTGTC GGACCAGCAC CGGCACCGGC ACCAGCACCG GCACCAGCAC CTTTGTACAA
              481 TTCATCCATA CCATGGGTAA TACCAGCAGC AGTAACAAAT TCTAACAAGA CCATGTGGTC
              541 TCTCTTTTCG TTTGGATCTT TGGATAANGC AGANTGAGTG GATAAGTAAT GGTTGTCTGG
              601 TAACAAGACT GGACCATCAC CAATTGGAGT ANTTTGTTGA TAATGNTCAG CTAATTGAAC
              661 AGAACCATCT TCNNTGNTGT GTCTNATTTT NAANTTNANT TTGATANNNN TCNTTNGNTN
            721 GNNNNNNNNN N
            //
            Primer: ADHpro_C (Pr0003)
            Region: insert mid (ADH1 pro,yeGFP 5')
            Sequence file: RDB08386_A2Bkb.seq check
            >08386_08386_B0JjF_1_ADH1pro_F_C01_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNTTT TGTTTCCTCG TCNTTGTTCT CGTTCCCTTT CTTCCTTGTT
               61 TCTTTTTCTG CACAATATTT CAAGCTATAC CAAGCATACA ATCAACTCCA AGCTGGCCGC
              121 TCTAGAACTA GTGGATCCCC CCCGGAATGT CTAAAGGTGA AGAATTATTC ACTGGTGTTG
              181 TCCCAATTTT GGTTGAATTA GATGGTGATG TTAATGGTCA CAAATTTTCT GTCTCCGGTG
              241 AAGGTGAAGG TGATGCTACT TACGGTAAAT TGACCTTAAA ATTTATTTGT ACTACTGGTA
              301 AATTGCCAGT TCCATGGCCA ACCTTAGTCA CTACTTTCGG TTATGGTGTT CAATGTTTTG
              361 CGAGATACCC AGATCATATG AAACAACATG ACTTTTTCAA GTCTGCCATG CCAGAAGGTT
              421 ATGTTCAAGA AAGAACTATT TTTTTCAAAG ATGACGGTAA CTACAAGACC AGAGCTGAAG
              481 TCAAGTTTGA AGGTGATACC TTAGTTAATA GAATCGAATT AAAAGGTATT GATTTTAAAG
              541 AAGANGGTAA CATTTTAGGT CACAAATTGG AATACAACTA TAACTCTCAC AATGTTTACA
              601 TCATGGCTGA CAAACAAAAG AATGGTATCA AAGTTAACTT CNAAATTAGA CACAACATTG
              661 AAGANGGTTC TGTTCNNTTA NCTGANNATT ATCNANNAAA NANTCCNNTT NNNNNNGGNN
            721 NNNNNNNN
            //
            Primer: pBAC_lac_R (Pr0545)
            Region: insert 5'
            Sequence file: RDB08386_A2Bkc.seq check
            >08386_08386_B0JjF_1_pBAC_lac_R_A01_01_ABI08.ab1
                1 NNNNNNNNNC NNNNNNAANG GGNNNAAAAG CTGGAGCTCG CCGGGNNCGA AGAAATGATG
               61 GTAAATGAAA TAGGAAATCA AGGAGCATGA AGGCAAAAGA CAAATATAAG GGTCGAACGA
              121 AAAATAAAGT GAAAAGTGTT GATATGATGT ATTTGGCTTT GCGGCGCCGA AAAAACGAGT
              181 TTACGCAATT GCACAATCAT GCTGACTCTG TGGCGGACCC GCGCTCTTGC CGGCCCGGCG
              241 ATAACGCTGG GCGTGAGGCT GTGCCCGGCG GAGTTTTTTG CGCCTGCATT TTCCAAGGTT
              301 TACCCTGCGC TAAGGGGCGA GATTGGAGAA GCAATAAGAA TGCCGGTTGG GGTTGCGATG
              361 ATGACGACCA CGACAACTGG TGTCATTATT TAAGTTGCCG AAAGAACCTG AGTGCATTTG
              421 CAACATGAGT ATACTAGAAG AATGAGCCAA GACTTGCGAG ACGCGAGTTT GCCGGTGGTG
              481 CGAACAATAG AGCGACCATG ACCTTGAAGG TGAGACGCGC ATAACCGCTA GAGTACTTTG
              541 AAGAGGAAAC AGCAATAGGG TTGCTACCAG TATAAATAGA CAGGTACATA CAACACTGGA
              601 AATGGTTGTC TGTTTGAGTA CGCTTTCAAT TCATTTGGGT GTGCACTTTA TTATGTTACA
              661 ATATGGAANG GAACTTTACA CTTCTCCTAN GCNNNTATNN TAATTAANNN CNNNNGNTAG
            721 TANNGNANNN GGGNNNNNNN CCNNNNNNNN NN
            //
            Primer: yeGFP-M_R (Pr0898)
            Region: insert mid (yeGFP 3',TEF ter)
            Sequence file: RDB08386_A2Bkd.seq check
            >08386_08386_B0JjF_1_yeGFP-M_R_A05_02_ABI24.ab1
                1 NNNNNNNNNN NNNCTNANTN NNNAGAATCG AATTAAAAGG TATTGATTTT AAAGAAGATG
               61 GTAACATTTT AGGTCACAAA TTGGAATACA ACTATAACTC TCACAATGTT TACATCATGG
              121 CTGACAAACA AAAGAATGGT ATCAAAGTTA ACTTCAAAAT TAGACACAAC ATTGAAGATG
              181 GTTCTGTTCA ATTAGCTGAC CATTATCAAC AAAATACTCC AATTGGTGAT GGTCCAGTCT
              241 TGTTACCAGA CAACCATTAC TTATCCACTC AATCTGCCTT ATCCAAAGAT CCAAACGAAA
              301 AGAGAGACCA CATGGTCTTG TTAGAATTTG TTACTGCTGC TGGTATTACC CATGGTATGG
              361 ATGAATTGTA CAAAGGTGCT GGTGCCGGTG CTGGTGCCGG TGCCGGTGCT GGTCCGACAG
              421 AGAATTCATC GATGATATCA GATCCACTAG TGGCCTATGC GGCCGCGTCG ACCTAATCAG
              481 CACTGACAAT AAAAAGATTC TTGTTTTCAA GAACTTGTCA TTTGTATAGT TTTTTTATAT
              541 TGTAGTTGTT CTATTTTAAT CAAATGTTAG CGTGATTTAT ATTTTTTTTC GCCTCNACAT
              601 CATCTGCCCA GATGCGAAGT TAAGTGCGCA NAAAGTAATA TCATGCGTNN ATCGNANGTG
              661 AATGCTGGTC GCTATACTGC TGTCNNNTCN ATACTAACGN CNNNATNNNN NGNCNAANNN
            721 NANCACTCNA NNNNCNNTNN NNNNNNNCNN NNNNNNNN
            //
            Primer: URA3pro_F (Pr0170)
            Region: URA3 pro,URA3
            Sequence file: RDB08386_A2Bke.seq check
            >08386_08386_B0JjF_1_URA3pro_F_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNTGNN NTTGCCNNGT ATTCTTAACC CAACTGCACA GAACAAAAAC
               61 CTGCAGGAAA CGAAGATAAA TCATGTCGAA AGCTACATAT AAGGAACGTG CTGCTACTCA
              121 TCCTAGTCCT GTTGCTGCCA AGCTATTTAA TATCATGCAC GAAAAGCAAA CAAACTTGTG
              181 TGCTTCATTG GATGTTCGTA CCACCAAGGA ATTACTGGAG TTAGTTGAAG CATTAGGTCC
              241 CAAAATTTGT TTACTAAAAA CACATGTGGA TATCTTGACT GATTTTTCCA TGGAGGGCAC
              301 AGTTAAGCCG CTAAAGGCAT TATCCGCCAA GTACAATTTT TTACTCTTCG AAGACAGAAA
              361 ATTTGCTGAC ATTGGTAATA CAGTCAAATT GCAGTACTCT GCGGGTGTAT ACAGAATAGC
              421 AGAATGGGCA GACATTACGA ATGCACACGG TGTGGTGGGC CCAGGTATTG TTAGCGGTTT
              481 GAAGCAGGCG GCAGAAGAAG TAACAAAGGA ACCTAGAGGC CTTTTGATGT TAGCAGAATT
              541 GTCATGCAAG GGCTCCCTAT CTACTGGAGA ATATACTAAG GGTACTGTTG ACATTGCGAA
              601 GAGCGACAAA GANTTTGTTA TCGGCTTTAT TGCTCAAAGA GACATGGGTG GAAGAGANGA
              661 ANNNTACGAN TGGTTGANTA TGACACCNNG NGTGGGTTTA GANGANNNNG GNNACGCATT
            721 GGNNCNNNNN NANNNNNNNN NN
            //
            Primer: pGEX-RSEQ (Pr0077)
            Region: CEN_ARS
            Sequence file: RDB08386_A2Bkf.seq check
            >08386_08386_B0JjF_1_pGEX-RSEQ_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNA NNGGCCTCGT GNNACGCCTA TTTTTATAGG TTAATGTCAT
               61 GATAATAATG GTTTCTTAGA CGGATCGCTT GCCTGTAACT TACACGCGCC TCGTATCTTT
              121 TAATGATGGA ATAATTTGGG AATTTACTCT GTGTTTATTT ATTTTTATGT TTTGTATTTG
              181 GATTTTAGAA AGTAAATAAA GAAGGTAGAA GAGTTACGGA ATGAAGAAAA AAAAATAAAC
              241 AAAGGTTTAA AAAATTTCAA CAAAAAGCGT ACTTTACATA TATATTTATT AGACAAGAAA
              301 AGCAGATTAA ATAGATATAC ATTCGATTAA CGATAAGTAA AATGTAAAAT CACAGGATTT
              361 TCGTGTGTGG TCTTCTACAC AGACAAGATG AAACAATTCG GCATTAATAC CTGAGAGCAG
              421 GAAGAGCAAG ATAAAAGGTA GTATTTGTTG GCGATCCCCC TAGAGTCTTT TACATCTTCG
              481 GAAAACAAAA ACTATTTTTT CTTTAATTTC TTTTTTTACT TTCTATTTTT AATTTATATA
              541 TTTATATTAA AAAATTTAAA TTATAATTAT TTTTATAGCA CGTGATGAAA AGGACCCAGG
              601 TGGCACTTTT CGGGGNAATG TGCGCGGAAC CCCTATTTGT TTANTTTTCT AAATACATTC
              661 NAATATGTAN CCGCTCATGA GACAATAACC NNGATAAATG CTTNNNNNNN ANTGNAAANN
            721 NNNNNTATGN NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Sasaki, T., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.

            2023.04.10

            GNP_filter3_RDBDEP_html_230405.pl