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pRS416-PADH1-ccdB-yEGFP-TTEF1

Empty expression vector backbone of S. cerevisiae

Catalog number RDB08380
Resource name pRS416-PADH1-ccdB-yEGFP-TTEF1
Clone info. Empty expression vector backbone of S. cerevisiae to express GFP-tagged protein. Gateway(R) destination vector.
Vector backbone pRS416-PADH1-ccdB-yEGFP-TTEF1 (Plasmid)
Size of vector backbone 9.2 kb
Selectable markers Amp^r
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor TAKAGI, Hiroshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Genes to cells, 18, 459-475 (2013)).
Ordering Please visit Information of Request for Distribution. 
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB08380 pRS416-PADH1-ccdB-yEGFP-TTEF1 Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08380z.seq from Depositor

Original reference

original Sasaki, T., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

Original, user report and related articles

original Sasaki, T., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.

2019.09.27

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