RDB08380 - pRS416-PADH1-ccdB-yEGFP-TTEF1

Resource data sheet
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pRS416-PADH1-ccdB-yEGFP-TTEF1

Empty expression vector backbone of S. cerevisiae

Catalog number	RDB08380
Resource name	pRS416-PADH1-ccdB-yEGFP-TTEF1
Clone info.	Empty expression vector backbone of S. cerevisiae to express GFP-tagged protein. Gateway(R) destination vector.
Vector backbone	pRS416-PADH1-ccdB-yEGFP-TTEF1 (Plasmid)
Size of vector backbone	9.2 kb
Selectable markers	Amp^r
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	TAKAGI, Hiroshi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Genes to cells, 18, 459-475 (2013)).

Ordering Please visit Information of Request for Distribution.[link]
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]

Remarks
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Genes to cells, 18, 459-475 (2013)).
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Genes to cells, 18, 459-475 (2013))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB08380	pRS416-PADH1-ccdB-yEGFP-TTEF1	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB08380z.seq

Original reference

original	Sasaki, T., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.

Further references such as user reports and related articles (go to bottom)

Featured content	Expression Vector Backbone of Saccharomyces cerevisiae (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Sasaki, T., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.

2020.10.15

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