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pRS416-PADH1-ccdB-yEGFP-TTEF1

Empty expression vector backbone of S. cerevisiae

Catalog number RDB08380
Resource name pRS416-PADH1-ccdB-yEGFP-TTEF1
Clone info. Empty expression vector backbone of S. cerevisiae to express GFP-tagged protein. Gateway(R) destination vector.
Vector backbone pRS416-PADH1-ccdB-yEGFP-TTEF1 (Plasmid)
Size of vector backbone 9.2 kb
Selectable markers Amp^r
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer TAKAGI, Hiroshi |
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External Database
            Reference sequence
              
             
            Sequence RDB08380z.seq

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Genes to cells, 18, 459-475 (2013)).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Genes to cells, 18, 459-475 (2013))。
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            RDB08380 pRS416-PADH1-ccdB-yEGFP-TTEF1 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Original reference

            original Sasaki, T., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Sasaki, T., Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.

            2023.04.10

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