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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMx-E4BP4 (Nfil3) (#RDB08379)

Expression clone of mouse Nfil3 cDNA

Alternative name Nfil3(E4BP4)/pMx-IRES-GFP
Clone info. Expression clone of mouse Nfil3 cDNA (NM_017373).
Vector backbone pMX-IRES-GFP (Plasmid)
Size of vector backbone 5.9 Kb
Selectable markers Amp^r
Gene/insert name Mouse Nfil3 cDNA
Depositor|Developer Taniguchi, Masaru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(Nature Immunology 12(5), 450-459, 2011).
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nature Immunology 12(5), 450-459, 2011)。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08379 pMx-E4BP4 (Nfil3) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMx-E4BP4 (Nfil3) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08379).

Reference section:

Motomura, Y., Kitamura, H., Hijikata, A., Matsunaga, Y., Matsumoto, K., Inoue, H., Atarashi, K., Hori, S., Watarai, H., Zhu, J., Taniguchi, M., Kubo, M., The transcription factor E4BP4 regulates the production of IL-10 and IL-13 in CD4+ T cells. Nature Immunology 12(5), 450-459, (2011). PMID 21460847. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08379_A5Bc.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5'
Sequence file: RDB08379_A5Bca.seq check
>08379_08379_A5Bc_pBABE5'_D11_11.ab1
    1 AGGGAAATGG CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGCTAA ACTTCCCTGA
   61 CCCTGACATG ACAAGAGTTA CTAACAGCCC CTCTCTCCAA GCTCACTTAC AGGCTCTCTA
  121 CTTAGTCCAG CACGAAGTCT GGAGACCTCT GGCGGCAGCC TACCAAGAAC AACTGGACCG
  181 ACCGGTGGTA CCTCACCCTT ACCGAGTCGG CGACACAGTG TGGGTCCGCC GACACCAGAC
  241 TAAGAACCTA GAACCTCGCT GGAAAGGACC TTACACAGTC CTGCTGACCA CCCCCACCGC
  301 CCTCAAAGTA GACGGCATCG CAGCTTGGAT ACACGCCGCC CACGTGAAGG CTGCCGACCC
  361 CGGGGGTGGA CCATCCTCTA GACTGCCGGA TCCACTAGTA ACGGCCGCCA GTGTGCTGGA
  421 ATTCGCCCTT CTGATGCAGC TGAGAAAAAT GCAGACCATC AAAAAGGAGC CCGCACCCCT
  481 AGATCCTACC AGCAGCTCAG ACAAGATGCT GCTGCTGAAC TCTGCCTTAG CTGAGGTGGC
  541 CGAGGACCTA GCCTCAGGTG AAGATTTGCT CCTGAACGAA GGGAGCATGG GGAAAAACAA
  601 ATCCTCGGCG TGTCGGAGAA AACGGGAATT CATTCCGGAC GAGAAGAAAG ACGCCATGTA
  661 TTGGGAGAAA CGGCGGAAAA ACAACGAAGC TGCCAAAAGA TCTCGGGAGA AGCGCCGCCT
  721 CAATGACCTG GTTTTGGAGA ACAAGCTGAT CGCCCTGGGA GAAGAAAATG CCACTTTAAA
  781 AGCTGAGCTG CTCTCCCTGA AATTAAAGTT TGGTTTAATT AGCTCCACGG CGTATGCCCA
  841 AGAAATCCAG AAACTCAGTA ATTCCACAGC TGTCTACTTT CAGGACTACC AGACATCCAA
  901 GGCTGCCGTG AGCTCTTTTG TGGACGAGCA TGAGCCTGCG ATGGTAGCCG GAAGTTGCAT
  961 CTCAGTCATC AAGCACTCTC CCCAGAGCTC GCTCTCCGAT GTGTCAGAGG TGTCCTCGGG
 1021 TGGGAGCACA CTCAGAAAGC CCCCGCACAG GGGAGGGCTG GCCGGAGCCT GAGACAGTTC
1081 CGTGTGATCA AGCAGAAGCC GTGTAGATGG GAGAGCTTTT GGCCAGGGAA A
//
Primer: IRES-R
Sequence file: RDB08379_A5Bcb.seq check
>08379_08379_A5Bc_IRES-R_E11_14.ab1
    1 GCCCCGCGGC TCGGCAGTAC GTTAGGGGGG GGGGAGGGAG AGGGGGGCGG GGGGGCGGAA
   61 TTTACGTAGC GGCCGCCAGG GGGATGGATA TCTGCAGAAT TCGCCCTTGC AGCCATTTAC
  121 CTGGAGTCCG AAGCCGAGAT CGGTTGTGTG GCTATGAATC TCTTGAGCGA GACCACCTCT
  181 GCAGATAAGT TTGCTATTCC CTGCTTCAAA TACAAATTCT CAGCTTCGGA AACCTTATAG
  241 CCACCGTCTT TGACTTCCAC CACACCTGTT TTGAAGCTAC TCTGAGTTTT GCTGCTCAGT
  301 TCTTTGTGAT GCCAGTGTTC CGATTTGAGG GACCAATCTT GAATGTTCGT CACCTGCACT
  361 GAGAAAGGAG GGAGGGAGGA ATGGGCCATA CCCGAGGTTC CATGTTTCTC CAGGTCAAAA
  421 TGTCTTTTGG CGATCGCATC GGCGGGTGAA GAGAGTTTCT GCATGCCTTC AAACTCGCTG
  481 TCCAAAGCCT CCACTTTGAC CTGCATGGCC TTGGCTTTAA TCCGAAGCTT GTGCGGTAAG
  541 GCAGAAGGGT TCACTTCCGG AACCTTCACC ACCGTGGCGT GGACCCGTTG CAGCTCCACT
  601 GGAGAATGGA TGGGGCCCTT AGGGACCTGT TGTTCGTCTT CCCCATCAGA AGACTTGCCC
  661 ACTACACCCT CATCGGCCTC TGAGGTTCTT GGGGAGTTGC TAGTGGACCC ATGGACCTGC
  721 AAGAGGGGTG GGGAGTGGGA GTAAGTGGAG AAAGAGCTTC CCATGTAGCT CTGGTAGATG
  781 GAGGTGGAAT ACGTGCCCCG CTCCTCCCTG GCCTCCCTGG CAAAGCTCTC CAACTCCACG
  841 GGCTCCTGCT TGATCACAGG GAACTTGTTC TCAGGGCTCC GGCAGCCTCC CTGTGCGGGG
  901 CTTTCCTGAG TGTGCTCCAC CGAGGACACC TCTGACACAT CGGAGAGCGA GCTCTGGGGA
  961 GAGTGCTTGA TGACTGAGAT GCAACTTCCG GCTACCATCG CAGGCTCATG CTCGTCCCAC
 1021 AAAAAGAGCT CACGGCCAGC CCTTTGGATG TCCTGGGTAG TTCCCTGGAA AGGTTAGAAC
 1081 AGCTGTGGAA TTACCTGAGT TCCTGATTTC GTGGCCAATA CGCCGGTGGA AAGCTTATTT
1141 AGGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Motomura, Y., The transcription factor E4BP4 regulates the production of IL-10 and IL-13 in CD4+ T cells. Nature Immunology 12(5), 450-459, (2011). PMID 21460847. [link to RRC of NBRP]