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NR6A1/TNT (#RDB08377)

Expression clone of human NR6A1 cDNA

Alternative name Human NR6A1
Clone info. Expression clone of human NR6A1 cDNA. Human NR6A1 cDNA was obtained from hypothalamic poly-A RNA by PCR amplification using primers containing XhoI and EcoRI, respictively. and cloned into the pCMVTNT vector.
Comment 5'-CCG CTC GAG CAT GGC GGA GCA ACA AAC-3'; 5'-GGA ATT CTC TTG CTA TGG AGG CAA CG-3'
Vector backbone pCMVTNT (Plasmid)
Size of vector backbone 4.1 kb
Selectable markers Amp^r
Gene/insert name Human NR6A1 cDNA
Depositor|Developer Tanaka, Susumu |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Biochem Biophys Res Commun, 403(2), 178-183, 2010).The availability of theBIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem Biophys Res Commun, 403(2), 178-183, 2010)。学術機関の学術研究に限る。利用者が本件リソースを使用して得られた研究成果に基づき特許権の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB08377 NR6A1/TNT Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The NR6A1/TNT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08377).

Reference section:

Tanaka, S., Kodama, T., Nonaka, T., Toyoda, H., Arai, M., Fukazawa, M., Honda, Y., Honda, M., Mignot, E., Transcriptional regulation of the hypocretin/orexin gene by NR6A1. Biochem. Biophys. Res. Commun. 403(2), 178-183, (2010). PMID 21056546. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tanaka, S., Transcriptional regulation of the hypocretin/orexin gene by NR6A1. Biochem. Biophys. Res. Commun. 403(2), 178-183, (2010). PMID 21056546. [link to RRC of NBRP]