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pCAG-mNr6a1 (#RDB08376)

Expression clone of mouse Nr6a1 cDNA

Alternative name Mouse Nr6a1
Clone info. Expression clone of mouse Nr6a1 cDNA. The CA promoter derived from pCA-pA expression vector was cloned into pcDNA3 vector (Invitrogen), resulting in pcaDNA3 vector (6.5 kb). Murine Nr6a1 was amplified from a cDNA clone using PCR and cloned into the pcaDNA3.
Vector backbone pcaDNA3 (Plasmid)
Size of vector backbone 6.5 kb
Selectable markers Amp^r
Gene/insert name Mouse Nr6a1 cDNA
Depositor|Developer Tanaka, Susumu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Biochem Biophys Res Commun, 403(2), 178-183, 2010).The availability of theBIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem Biophys Res Commun, 403(2), 178-183, 2010)。学術機関の学術研究に限る。利用者が本件リソースを使用して得られた研究成果に基づき特許権の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB08376 pCAG-mNr6a1 Under QC test. Please contact us. DNA solution Please contact us.

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How to cite this biological resource

Materials & Methods section:

The pCAG-mNr6a1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08376).

Reference section:

Tanaka, S., Kodama, T., Nonaka, T., Toyoda, H., Arai, M., Fukazawa, M., Honda, Y., Honda, M., Mignot, E., Transcriptional regulation of the hypocretin/orexin gene by NR6A1. Biochem. Biophys. Res. Commun. 403(2), 178-183, (2010). PMID 21056546. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tanaka, S., Transcriptional regulation of the hypocretin/orexin gene by NR6A1. Biochem. Biophys. Res. Commun. 403(2), 178-183, (2010). PMID 21056546. [link to RRC of NBRP]