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CS-CDF-UbCG-PRE (#RDB08363)

Lentivirus vector plasmid of GFP driven by UbC promoter.

Clone info. Lentivirus vector plasmid of GFP driven by UbC promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB08363z.seq
Remarks, protocol and/or map (pdf) RDB08363.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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RDB08363 CS-CDF-UbCG-PRE DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-CDF-UbCG-PRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08363).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17485_A9I4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-M_R (Pr0743)
Region: EGFP,Ubc pro
Sequence file: RDB17485_A9I4a.seq check
>08363_17485_A9I4_3_EGFP-M_R_G08_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNGGNNCTT GNNGTTNNCC TTNNATGCCG TTCTTCTGCT
   61 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
  121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
  181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
  241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
  301 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG
  361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT
  421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA
  481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GTGGCGACCG
  541 GTAGCGCTAA ACACGGTCCG GTCTAACAAA AAAGCCAAAA ACGGCCAGAA TTTAGCGGAC
  601 AATTTACTAG TCTAACACTG AAAATTACAT ATTGACCCAA ATGATTACAT TTCAAAAGGT
  661 GCCTAAAAAA CTTCACAAAA CACACTCGCC AACCCCGAGC GCATAGTTCA AAACCGGAGC
  721 TTCAGCTACT TAAGAAGATA GGTACATAAA ACCGACCAAA GAAACTGACG CCTCACTTAT
  781 CCCTCCCCTC ACCAGANGTC CGGCGCCTGT CGATTCNNGA NAGCCTACCC TANGNCCGAA
  841 CCCNGCGTCC TGCGACNGAN AAAAGCCTAC NGCACACCTA CCGGCAGGNG GNCCNNCCCN
  901 GCATTATAAN NCNNNCANAA CGGGTGANGT CNCGACACGA CNANGGCGCG CGCNCCCNNN
961 NNNACNGGNG CACTGNCCNN NNNNNCCGNC NTNNCTGNNG NCCCCNNNNN NGA
//
Primer: PRE_rev (Pr0250)
Region: WPRE,EGFP
Sequence file: RDB17485_A9I4b.seq check
>08363_17485_A9I4_3_PRE_rev_H11_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNNN GGANCNNCNT AGTTAAGAAT ACCNGTCAAT CTTTNNCAAA
   61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGAGATCCT ACTAGTAGGA
  121 TCTGAGTCCG GACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA
  181 CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT
  241 GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG
  301 GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC
  361 CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA
  421 CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT
  481 GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC
  541 GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA
  601 GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT
  661 GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT
  721 CAGCTTGCCG TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT
  781 TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT
  841 GCTCACCATG GNGGCGACCG GTAGCGCTAA ACACGGNCCG GTCTNACAAA AAAGCCNAAA
  901 ACGGNCAGAA TTTANCGGAN NATTTACTAG TCNNNNNCTN NAANTTACNT ANNGACCCNA
961 NNGNNTACNT TTNCNAANNN NGNCNNANAA ANNTTCNNNA A
//
Primer: CMV enh_F1 (Pr0652)
Region: CMV pro,5'LTR,HIV-1psi
Sequence file: RDB17485_A9I4c.seq check
>08363_17485_A9I4_3_CMVenh_F1_A12_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCNNNGNN NNTGNCCTTA TGGGNNTTTC CTACTTGGCA
   61 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA
  121 TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA
  181 TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC
  241 CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGCGCGTT
  301 TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC
  361 TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG
  421 CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA
  481 AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC CAGAGGAGCT
  541 CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG GGCGGCGACT
  601 GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG GGTGCGAGAG
  661 CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT TAAGGCCAGG
  721 GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT
781 CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAANGNTGT
//
Primer: RRE-M_R (Pr0651)
Region: RRE,HIV-1psi
Sequence file: RDB17485_A9I4d.seq check
>08363_17485_A9I4_3_RRE-M_R_B12_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCTGTTGNGC CTCNNTAGCC CTCNNNCAAA TTGTTCTGCT
   61 GCTGCACTAT ACCAGACAAT AATTGTCTGG CCTGTACCGT CAGCGTCATT GACGCTGCGC
  121 CCATAGTGCT TCCTGCTGCT CCCAAGAACC CAAGGAACAA AGCTCCTATT CCCACTGCTC
  181 TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG TGCTACTCCT AATGGTTCAA
  241 TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT GTCCCTCATA TCTCCTCCTC
  301 CAGGTCTGAA GATCAGCGGC CGCTTGCTGT GCGGTGGTCT TACTTTTGTT TTGCTCTTCC
  361 TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA TCCTTTGATG CACACAATAG
  421 AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG ATCCTGTCTG AAGGGATGGT
  481 TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT CTAACAGGCC AGGATTAACT
  541 GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT TTAATTTATA TTTTTTCTTT
  601 CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA TTCTCCCCCG CTTAATACTG
  661 ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG TCAAAATTTT TGGCGTACTC
  721 ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG CAAGCCGAGT CCTGCGTCGA
781 GAGAGCTCCT CTGGTTTCCN TTTCGCTTTC NAG
//
Primer: BGH_rev2 (Pr0606)
Region: bGHp(A),3'LTR
Sequence file: RDB17485_A9I4e.seq check
>08363_17485_A9I4_3_BGH_rev2_G11_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG GGGNNGGNNN NNNACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
  121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
  181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
  241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC
  301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC
  361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC
  421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA
  481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA
  541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC
  601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG
  661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA
  721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG
781 ACNGTATCGA TGCGGGGAN
//
Primer: pAxEF R2 (Pr0059)
Region: SV40p(A),Zeo,SV40pro_ori
Sequence file: RDB17485_A9I4f.seq check
>08363_17485_A9I4_3_pAxEFR2_C12_09_ABI24.ab1
    1 NNNNNNNNNN NNNNTNNTTT GTGNANTTGT GNNGCTATTG CTTTATTTGT AACCATTATA
   61 AGCTGCAATA AACAAGTTGG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG
  121 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC
  181 GGTGGAATCG AAATCTCGTA GCACGTGTCA GTCCTGCTCC TCGGCCACGA AGTGCACGCA
  241 GTTGCCGGCC GGGTCGCGCA GGGCGAACTC CCGCCCCCAC GGCTGCTCGC CGATCTCGGT
  301 CATGGCCGGC CCGGAGGCGT CCCGGAAGTT CGTGGACACG ACCTCCGACC ACTCGGCGTA
  361 CAGCTCGTCC AGGCCGCGCA CCCACACCCA GGCCAGGGTG TTGTCCGGCA CCACCTGGTC
  421 CTGGACCGCG CTGATGAACA GGGTCACGTC GTCCCGGACC ACACCGGCGA AGTCGTCCTC
  481 CACGAAGTCC CGGGAGAACC CGAGCCGGTC GGTCCAGAAC TCGACCGCTC CGGCGACGTC
  541 GCGCGCGGTG AGCACCGGAA CGGCACTGGT CAACTTGGCC ATGGTTTAGT TCCTCACCTT
  601 GTCGTATTAT ACTATGCCGA TATACTATGC CGATGATTAA TTGTCAACAC GTGCTGATCA
  661 GATCCGAAAA TGGATATACA AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA
721 AAGCCTCCTC ACTACTTCTG GAATAGCTCA GANGCAGANG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Kayamori, K., DHODH inhibition synergizes with DNA-demethylating agents in the treatment of myelodysplastic syndromes. Blood Adv. 5 (2): 438-450 (2021). PMID 33496740. [link to RRC of NBRP]
user_report Zhong, C., Efficacy of the novel tubulin polymerization inhibitor PTC-028 for myelodysplastic syndrome. Cancer Sci. 111 (12): 4336-4347 (2020). PMID 33037737. [link to RRC of NBRP]