Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-CDF-UbCG-PRE

Lentivirus vector plasmid of GFP driven by UbC promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose)[Word] Please visit Ordering instruction.[link]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17485_A9I4p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-M_R (Pr0743) Region: EGFP,Ubc pro Sequence file: RDB17485_A9I4a.seq
>08363_17485_A9I4_3_EGFP-M_R_G08_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNGGNNCTT GNNGTTNNCC TTNNATGCCG TTCTTCTGCT    61 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA   121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC   181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC   241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT   301 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG   361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT   421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA   481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GTGGCGACCG   541 GTAGCGCTAA ACACGGTCCG GTCTAACAAA AAAGCCAAAA ACGGCCAGAA TTTAGCGGAC   601 AATTTACTAG TCTAACACTG AAAATTACAT ATTGACCCAA ATGATTACAT TTCAAAAGGT   661 GCCTAAAAAA CTTCACAAAA CACACTCGCC AACCCCGAGC GCATAGTTCA AAACCGGAGC   721 TTCAGCTACT TAAGAAGATA GGTACATAAA ACCGACCAAA GAAACTGACG CCTCACTTAT   781 CCCTCCCCTC ACCAGANGTC CGGCGCCTGT CGATTCNNGA NAGCCTACCC TANGNCCGAA   841 CCCNGCGTCC TGCGACNGAN AAAAGCCTAC NGCACACCTA CCGGCAGGNG GNCCNNCCCN   901 GCATTATAAN NCNNNCANAA CGGGTGANGT CNCGACACGA CNANGGCGCG CGCNCCCNNN 961 NNNACNGGNG CACTGNCCNN NNNNNCCGNC NTNNCTGNNG NCCCCNNNNN NGA //
Primer: PRE_rev (Pr0250) Region: WPRE,EGFP Sequence file: RDB17485_A9I4b.seq
>08363_17485_A9I4_3_PRE_rev_H11_23_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNNN GGANCNNCNT AGTTAAGAAT ACCNGTCAAT CTTTNNCAAA    61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGAGATCCT ACTAGTAGGA   121 TCTGAGTCCG GACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA   181 CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT   241 GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG   301 GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC   361 CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA   421 CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT   481 GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC   541 GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA   601 GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT   661 GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT   721 CAGCTTGCCG TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT   781 TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT   841 GCTCACCATG GNGGCGACCG GTAGCGCTAA ACACGGNCCG GTCTNACAAA AAAGCCNAAA   901 ACGGNCAGAA TTTANCGGAN NATTTACTAG TCNNNNNCTN NAANTTACNT ANNGACCCNA 961 NNGNNTACNT TTNCNAANNN NGNCNNANAA ANNTTCNNNA A //
Primer: CMV enh_F1 (Pr0652) Region: CMV pro,5'LTR,HIV-1psi Sequence file: RDB17485_A9I4c.seq
>08363_17485_A9I4_3_CMVenh_F1_A12_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGCNNNGNN NNTGNCCTTA TGGGNNTTTC CTACTTGGCA    61 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA   121 TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA   181 TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC   241 CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGCGCGTT   301 TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC   361 TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG   421 CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA   481 AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC CAGAGGAGCT   541 CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG GGCGGCGACT   601 GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG GGTGCGAGAG   661 CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT TAAGGCCAGG   721 GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT 781 CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAANGNTGT //
Primer: RRE-M_R (Pr0651) Region: RRE,HIV-1psi Sequence file: RDB17485_A9I4d.seq
>08363_17485_A9I4_3_RRE-M_R_B12_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GCTGTTGNGC CTCNNTAGCC CTCNNNCAAA TTGTTCTGCT    61 GCTGCACTAT ACCAGACAAT AATTGTCTGG CCTGTACCGT CAGCGTCATT GACGCTGCGC   121 CCATAGTGCT TCCTGCTGCT CCCAAGAACC CAAGGAACAA AGCTCCTATT CCCACTGCTC   181 TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG TGCTACTCCT AATGGTTCAA   241 TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT GTCCCTCATA TCTCCTCCTC   301 CAGGTCTGAA GATCAGCGGC CGCTTGCTGT GCGGTGGTCT TACTTTTGTT TTGCTCTTCC   361 TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA TCCTTTGATG CACACAATAG   421 AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG ATCCTGTCTG AAGGGATGGT   481 TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT CTAACAGGCC AGGATTAACT   541 GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT TTAATTTATA TTTTTTCTTT   601 CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA TTCTCCCCCG CTTAATACTG   661 ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG TCAAAATTTT TGGCGTACTC   721 ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG CAAGCCGAGT CCTGCGTCGA 781 GAGAGCTCCT CTGGTTTCCN TTTCGCTTTC NAG //
Primer: BGH_rev2 (Pr0606) Region: bGHp(A),3'LTR Sequence file: RDB17485_A9I4e.seq
>08363_17485_A9I4_3_BGH_rev2_G11_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG GGGNNGGNNN NNNACAGATG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA   121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG   181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC   241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC   301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC   361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC   421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA   481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA   541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC   601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG   661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA   721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG 781 ACNGTATCGA TGCGGGGAN //
Primer: pAxEF R2 (Pr0059) Region: SV40p(A),Zeo,SV40pro_ori Sequence file: RDB17485_A9I4f.seq
>08363_17485_A9I4_3_pAxEFR2_C12_09_ABI24.ab1     1 NNNNNNNNNN NNNNTNNTTT GTGNANTTGT GNNGCTATTG CTTTATTTGT AACCATTATA    61 AGCTGCAATA AACAAGTTGG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG   121 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC   181 GGTGGAATCG AAATCTCGTA GCACGTGTCA GTCCTGCTCC TCGGCCACGA AGTGCACGCA   241 GTTGCCGGCC GGGTCGCGCA GGGCGAACTC CCGCCCCCAC GGCTGCTCGC CGATCTCGGT   301 CATGGCCGGC CCGGAGGCGT CCCGGAAGTT CGTGGACACG ACCTCCGACC ACTCGGCGTA   361 CAGCTCGTCC AGGCCGCGCA CCCACACCCA GGCCAGGGTG TTGTCCGGCA CCACCTGGTC   421 CTGGACCGCG CTGATGAACA GGGTCACGTC GTCCCGGACC ACACCGGCGA AGTCGTCCTC   481 CACGAAGTCC CGGGAGAACC CGAGCCGGTC GGTCCAGAAC TCGACCGCTC CGGCGACGTC   541 GCGCGCGGTG AGCACCGGAA CGGCACTGGT CAACTTGGCC ATGGTTTAGT TCCTCACCTT   601 GTCGTATTAT ACTATGCCGA TATACTATGC CGATGATTAA TTGTCAACAC GTGCTGATCA   661 GATCCGAAAA TGGATATACA AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA 721 AAGCCTCCTC ACTACTTCTG GAATAGCTCA GANGCAGANG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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