Resource data sheet
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CS-RfA-ETR

Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-mRFP1 marker driven by EF-1 alpha promoter.

Catalog number RDB08362
Resource name CS-RfA-ETR
Clone info. Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-mRFP1 marker driven by EF-1 alpha promoter.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-RfA-ETR ()
Selectable markers Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. Please contact us. UCSD's MTA is also required.
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Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08362 CS-RfA-ETR DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08362z.seq from Depositor
Electronic file PDF RDB08362.pdf from Depositor

Featured content

References

original Suga, H., Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480 (7375): 57-62 (2011). PMID 22080957.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12396_A4Hcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R RDB12396_A4Hca_2.seq
>08362_12396_A4Hc_A_1_Cm-M_R_C10_07.ab1
    1 GTCAGGGGTG ACCTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAATTCC
   61 GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA
  121 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA
  181 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA
  241 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC
  301 GACGGATCCT AACTCAAAAT CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
  361 GGGGTGCCTA ATGCGGCCGC CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG
  421 TCTGTTTTTT ATGCAAAATC TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC
  481 GTTCAGCTTT TTTGTACAAA CTTGTGATAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT
  541 AAACCCGAAA ATTTTGAATT TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG
  601 TTGCTATTAT GTCTACTATT CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT
  661 TAAAATTGTG GATGAATACT GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT
  721 CTGTCTCTCT CTCCACCTTC TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT
  781 GGGAGGTGGG TCTGAAACGA TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA
  841 AGTACAGCAA AAACTATTCT TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT
  901 ATATACCACA GCCAATTTGT TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT
  961 TCCAATAATT TCTTGTTCAT TCTTTTCTTG CTGGGTTTGC GATTCTTCAA TTAAAGGAGT
 1021 GTATTAAGCT TGTGTAATTG TTAATTTTCT TCTGTCCCAC TCCATCCAGG TCGTGTGATT
 1081 CAAATCTGTT CAGAGATTTA TTTACTTCCA CCTAGGCCAT TCGAGCACAA GCCATGTGCC
1141 AATGAAGTTC GAGCTAGCCA ATTCCCAGAC AGGTCT
//
Primer: EF1a-N_R RDB12396_A4Hcb_2.seq
>08362_12396_A4Hc_A_1_EF1a-N_R_B10_04.ab1
    1 TTTTCCCTAT TTTTCGGGGG GGAAGGTGGG GGGGGATGTG CGCTCTGCCC ACTGCCGGGG
   61 CACCGGAGCC TCACGAAGCT TGAATTAAAA AAAAAAAGTT GTACAAGAAA GCTGAACGAG
  121 AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA
  181 CATAATACTG TAAAACACAA CATATCCAGT CACTATGGTC GACCTGCAGA CTGGCTGTGT
  241 ATAAGGGAGC CTGACATTTA TATTCCCCAG AACATCAGGT TAATGGCGTT TTTGATGTCA
  301 TTTTCGCGGT GGCTGAGATC AGCCACTTCT TCCCCGATAA CGGAGACCGG CACACTGGCC
  361 ATATCGGTGG TCATCATGCG CCAGCTTTCA TCCCCGATAT GCACCACCGG GTAAAGTTCA
  421 CGGGAGACTT TATCTGACAG CAGACGTGCA CTGGCCAGGG GGATCACCAT CCGTCGCCCG
  481 GGCGTGTCAA TAATATCACT CTGTACATCC ACAAACAGAC GATAACGGCT CTCTCTTTTA
  541 TAGGTGTAAA CCTTAAACTG CATTTCACCA GCCCCTGTTC TCGTCAGCAA AAGAGCCGTT
  601 CATTTCAATA AACCGGGCGA CCTCAGCCAT CCCTTCCTGA TTTTCCGCTT TCCAGCGTTC
  661 GGCACGCAGA CGACGGGCTT CATTCTGCAT GGTGTGCTTA CCAGACCGGA GATATTGACA
  721 TGTATATGCC TTGAGCAACT GATAGCTTGT CGCTGTCAAC TGTCACTGTA ATACGCTGCT
  781 TCATAGCCAT ACCTCTTTTT TGACATACTT TCGGGGTATA CAATATCAGT ATATATTCTT
  841 ATACCGCAAA AATCAGCGCG CAAATACGCA ATACTGTTAT CTGGGCTTTT AGTAAAGCCG
  901 GGATCCACGC GTTTACGCCC CGCCCCTGCC ACTCAATCGC AAGTACTGTT GTAATTCATT
  961 AAGCCATTCT GCCCGACATG GAAGCCATTC ACAAACGGGC ATGATGGACC CTGAATTCGC
 1021 CAGGCGGCAT CAGCACCCTT GTCGCCCTTG CCGTAATAAA TAATTTGCCC CAATGGGTGA
 1081 AAAACGGGGG GCCGAAGAAG TTTGTCCCAT AATTGGGCCC ACCGTTTTAT AAATCCAAAA
 1141 ACTTGGGTGA AACTTCACCC CCCCAGGGAA TTGGGCCTGA GAAATCGAAA AAATCCATAT
1201 ATTTTCCTCA ATTTTAAACC CCTTTAAGAG GGACAAAAAT AATAGTGGGC GC
//
Primer: pAxEF_F2 RDB12396_A4Hcc_2.seq
>08362_12396_A4Hc_A_1_pAxEF_F2_A10_01.ab1
    1 GTTTCCTTCC TTTCAGGTGT CGTGAATTAA TTAACCGGCC GCATAACCGT GGCGGTGGCA
   61 GCTAGACTAG CGCTACCGGT CGCCACCATG TCTAGATTAG ATAAAAGTAA AGTGATTAAC
  121 AGCGCATTAG AGCTGCTTAA TGAGGTCGGA ATCGAAGGTT TAACAACCCG TAAACTCGCC
  181 CAGAAGCTAG GTGTAGAGCA GCCTACATTG TATTGGCATG TAAAAAATAA GCGGGCTTTG
  241 CTCGACGCCT TAGCCATTGA GATGTTAGAT AGGCACCATA CTCACTTTTG CCCTTTAGAA
  301 GGGGAAAGCT GGCAAGATTT TTTACGTAAT AACGCTAAAA GTTTTAGATG TGCTTTACTA
  361 AGTCATCGCG ATGGAGCAAA AGTACATTTA GGTACACGGC CTACAGAAAA ACAGTATGAA
  421 ACTCTCGAAA ATCAATTAGC CTTTTTATGC CAACAAGGTT TTTCACTAGA GAATGCATTA
  481 TATGCACTCA GCGCTGTGGG GCATTTTACT TTAGGTTGCG TATTGGAAGA TCAAGAGCAT
  541 CAAGTCGCTA AAGAAGAAAG GGAAACACCT ACTACTGATA GTATGCCGCC ATTATTACGA
  601 CAAGCTATCG AATTATTTGA TCACCAAGGT GCAGAGCCAG CCTTCTTATT CGGCCTTGAA
  661 TTGATCATAT GCGGATTAGA AAAACAACTT AAATGTGAAA GTGGGTCCGC GTACAGCGGA
  721 TCCGGCGAGG GCAGAGGAAG TCTTCTAACA TGCGGTGACG TGGAGGAAAA TCCCGGGCCT
  781 ATGGCCTCCT CCGAGGACGT CATCAAGGAG TTCATGCGCT TCAAGGTGCG CATGGAGGGC
  841 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC
  901 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGCGGCCCCC TGCCCTTCGC CTGGGACATC
  961 CTGTCCCCTC AGTTCCAGTA CGGCTCCAAG GCCTACGTGA AGCACCCCCG CCGACATCCC
 1021 CGACTACTTG AAGCTGTCCT TTCCCGAAGG GCTTCCAGTG GGAGCGCGGT GAATGAACTT
 1081 CGAGGAACGG CGCGTGTGAC CGTGACCAGG GACTCCTCCT GCAGAACGCC GAGTTCAATC
1141 TACAGTTGAG CCTGCCGACT ACTCCCTCGA CCGTCTACT
//
Primer: PRE_rev RDB12396_A4Hcd_2.seq
>08362_12396_A4Hc_PRE_rev_D10_10.ab1
    1 ACGCATCCAA TAGCGTAAAA GGAGCACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGAGTGCGGC CGCTTAGGCG
  121 CCGGTGGAGT GGCGGCCCTC GGCGCGCTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG
  181 TGGGAGGTGA TGTCCAGCTT GATGTCGGTC TTGTAGGCGC CGGGCAGCTG CACGGGCTTC
  241 TTGGCCATGT AGGTGGTCTT GACCTCGGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC
  301 CTCATCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC GGTGGAGGCC
  361 TCCCAGCCCA TGGTCTTCTT CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC
  421 AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC
  481 ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCACTTGA AGCCCTCGGG GAAGGACAGC
  541 TTCAAGTAGT CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACTGGAAC
  601 TGAGGGGACA GGATGTCCCA GGCGAAGGGC AGGGGGCCGC CCTTGGTCAC CTTCAGCTTG
  661 GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC CCTCGATCTC GAACTCGTGG
  721 CCGTTCACGG AGCCCTCCAT GCGCACCTTG AAGCGCATGA ACTCCTTGAT GACGTCCTCG
  781 GAGGAGGCCA TAGGCCCGGG ATTTTCCTCC ACGTCACCGC ATGTTAGAAG ACTTCCTCTG
  841 CCCTCGCCGG ATCCGCTGTA CGCGGACCCA CTTTCACATT TAAGTTGTTT TTCTAATCCG
  901 CATATGATCA ATTCAAAGGC CGAATAAAGA ACGGCTGGCT CTGCACCCTT TGGTGATCAA
  961 ATAAATTTCG ATAGCTTGTC GTAATAAATG ACGGGCCATA CTATTCAGGT TAGTTAGGTG
1021 ATTCCCCTTT CGTCGTTTAG GCCGACTTGA ATGCTCTTGG ATTCGTTCCA ATAACGC
//
Primer: CMV-Forward RDB12396_A4Hce_2.seq
>08362_12396_A4Hc_CMV-Forward_A04_01.ab1
    1 ATTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGCGCGTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC
  121 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT
  181 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC
  241 AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA
  301 GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG
  361 CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG
  421 TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA
  481 AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA
  541 GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG
  601 GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA
  661 GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA
  721 GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC GGCCGCTGAT
  781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA
  841 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA
  901 GAGAGAAAAA AAGAGCAGTG GGGAATAGGA GCTTTTGTTT CCTTTGGGTT TCTTGGGGAG
  961 CAGCAGGAAA GCACTATGGG GCGCAGCGTC AATGACGCTG GACGGTACAG GCCCAGACAA
 1021 ATTTATTGTT CTGGTTATAG CTGCAGCAGC AGCACCACTT TGGCTGAACG GACCTTATTT
1081 GAAGGACGGC A
//
Primer: BGH_rev RDB12396_A4Hcf_2.seq
>08362_12396_A4Hc_BGHrev_B04_04.ab1
    1 GGATCGGCAT GCTAGAGATT TTCCACACTG ACTAAAGGGT CTGAGGGATC TCTAGTTACC
   61 AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC TTTATTGAGG
  121 CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT GGTCTAACCA
  181 GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG TGATGAAATG CTAGGCGGCT
  241 GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC ATCCCATGCA GGCTCACAGG
  301 GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT ACCTTCTCTT GCTCAACTGG
  361 TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG ATCCCTGGCC CTGGTGTGTA
  421 GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC CACAGATCAA GGATATCTTG
  481 TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG
  541 ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGTACC TGAGGTGTGA CTGGAAAACC
  601 CACCTCCTCC TCCTCTTGTG CTTCTAGCCA GGCACAATCA GCATTGGTAG CTGCTGTATT
  661 GCTACTTGTG ATTGCTCCAT GTTTTTCTAG GTCTCGACGG TATCGATGCG GGGAGGCGGC
  721 CCAAAGGGAG ATCCGACTCG TCTGAGGGCG AAGGCGAAGA CGCGGAAGAG GCCGCAGAGC
  781 CGGCAGCAGC CGCGGGAAAG GAAGGTCCGC TGGATTGAGG GCCGAAGGGA CGTAGCAGAA
  841 GGACGTCCCG CGCAGAATCC AGGTGGCAAC ACAGGCGAGC AGCCATGGAA AAGGACGTCA
  901 GCTTCCCCGA CACACCACGG GAATGTCAGT GCCCACAGCC GGAGCCCCTG TCCAGCAGCG
  961 GGCAGCAGCG CGATGAGTCG TCGTGCATAG GGAGGGGGAA AGCGAAGTTC CGAAGGACTG
1021 AACTGGTGGT GGCATGCCTA TCATTGGAAT GCTCAGCACC AGGCAACCAG GCCACGATC
//
Primer: SV40pro_F RDB12396_A4Hcg_2.seq
>08362_12396_A4Hc_SV40pro_F_C04_07.ab1
    1 CACGTTCTGG ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
  121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
  181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
  241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
  301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
  361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
  421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
  481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
  541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
  661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
  841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
  901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
  961 AAATTGCGTT GCGCTCACTG CCCGCTTTCC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT
 1021 TAATGAATCC GGCCACCGCC GCCGGGGCAG AGGCCGATTG CGTAATGGGG GCGCTTCATT
1081 CCGCCTTTTC CATTCG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Suga, H., Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480 (7375): 57-62 (2011). PMID 22080957.
reference Takata, N., Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9. Hum. Gene Ther. 27 (6): 436-450 (2016). PMID 26839115.
user report Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219.
user report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
user report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
user report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Advances 1: 1440-1451 (2017). PMID 29296785.

2018.10.22

GNP_filter3_RDBDEP_html_181012.pl