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CS-UbC-RfA-IRES2-hKO1 (#RDB08350)

Backbone vector plasmid of lentivirus construct driven by UbC promoter by Gateway(R) cloning with hKO1 fluorescent protein marker.

Clone info. Backbone vector plasmid of lentivirus construct driven by UbC promoter by Gateway(R) cloning with hKO1 fluorescent protein marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CS-UbC-RfA-IRES2-hKO1 (Plasmid)
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB08350z.seq
Remarks, protocol and/or map (pdf) RDB08350.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08350 CS-UbC-RfA-IRES2-hKO1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-UbC-RfA-IRES2-hKO1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08350).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14535_A7Ajp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: UbCpro_F (Pr0158)
Region: UbC pro,attR1,CmR
Sequence file: RDB14535_A7Aja.seq check
>08350_14535_A7Aj_1_UbCpro_F_A07_01_ABI24.ab1
    1 AGCTTGTATT TTCAGTGTTA GACTAGTAAA TTGTCCGCTA AATTCTGGCC GTTTTTGGCT
   61 TTTTTGTTAG ACCGGACCGT GTTTAGCGCT ACCGGTCTCG AGAATTCTGC AGCGGCCATC
  121 ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT CAATATATTA
  181 AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA TATCCAGTCA
  241 CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATAATG
  301 TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT AAAATGGAGA
  361 AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA GAACATTTTG
  421 AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG GATATTACGG
  481 CCTTTTTAAA GACCGTAAAG AAAAATAAGC ACAAGTTTTA TCCGGCCTTT ATTCACATTC
  541 TTGCCCGCCT GATGAATGCT CATCCGGAAT TCCGTATGGC AATGAAAGAC GGTGAGCTGG
  601 TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA TGAGCAAACT GAAACGTTTT
  661 CATCGCTCTG GAGTGAATAC CACGACGATT TCCGGCAGTT TCTACACATA TATTCGCAAG
  721 ATGTGGCGTG TTACGGTGAA AACCTGGCCT ATTTCCCTAA AGGGTTTATT GAGAATATGT
  781 TTTTCGTCTC AGCCAATCCC TGGGTGAGTT TCACCAGTTT TGATTTAAAC GTGGCCAATA
  841 TGGACAACTT CTTCGCCCCC GTTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG
  901 GTGCTGATGC CGCTGGCGAT TCAGTTCATC ATGCCGTTTG TGATGGCTTC CATGTCGGCA
  961 GAATGCTTAA TGAATTACAA CAGTACTGCG ATGAGTGGGC AGGCGGGGCG TAAACGCGTG
 1021 GATCCCGGCT TACTAAAAAG CCAGATACAG TATGCGTATT TTGCGCGCTG ATTTTTTGCG
 1081 GTATAAGATA TATACTGATA TGTATACCGG AAGTATGGTC AAAAAGGAGG GTAATGCCTA
//
Primer: IRES-R (Pr0037)
Region: IRES2,attR2,ccdB
Sequence file: RDB14535_A7Ajb.seq check
>08350_14535_A7Aj_IRES-R_G10_07_ABI08.ab1
    1 GGCATCGCGG CCTTCGGCAG TACGTTAGGG GGGGGGGAGG GAGAGGGGCG GATCCGCGGC
   61 CATCACCACT TTGTACAAGA AAGCTGAACG AGAAACGTAA AATGATATAA ATATCAATAT
  121 ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC AACATATCCA
  181 GTCACTATGG TCGACCTGCA GACTGGCTGT GTATAAGGGA GCCTGACATT TATATTCCCC
  241 AGAACATCAG GTTAATGGCG TTTTTGATGT CATTTTCGCG GTGGCTGAGA TCAGCCACTT
  301 CTTCCCCGAT AACGGAGACC GGCACACTGG CCATATCGGT GGTCATCATG CGCCAGCTTT
  361 CATCCCCGAT ATGCACCACC GGGTAAAGTT CACGGGAGAC TTTATCTGAC AGCAGACGTG
  421 CACTGGCCAG GGGGATCACC ATCCGTCGCC CGGGCGTGTC AATAATATCA CTCTGTACAT
  481 CCACAAACAG ACGATAACGG CTCTCTCTTT TATAGGTGTA AACCTTAAAC TGCATTTCAC
  541 CAGCCCCTGT TCTCGTCAGC AAAAGAGCCG TTCATTTCAA TAAACCGGGC GACCTCAGCC
  601 ATCCCTTCCT GATTTTCCGC TTTCCAGCGT TCGGCACGCA GACGACGGGC TTCATTCTGC
  661 ATGGTTGTGC TTACCAGACC GGAGATATTG ACATCATATA TGCCTTGAGC AACTGATAGC
  721 TGTCGCTGTC AACTGTCACT GTAATACGCT GCTTCATAGC ATACCTCTTT TTGACATACT
  781 TCGGGTATAC ATATCAGTAT ATATTCTTAT ACCGCAAAAA TCAGCGCGCA AATACGCATA
  841 CTGTTATCTG GGCTTTTAGT AAGCCGGATC CACGCGTTTA CGCCAGCCGC CCCTGCCACT
  901 CATCGCAGTA CTGTTTGTAA ATTCATTTAA AGCATTTCTG CCCGACATGG GAAAGCCCAA
  961 TCACAAACGG GCCATGGATT GAAACCTGGA AATCGCCAGC GGGCATCAGC ACCCTTTGGT
 1021 CCGCCCTTGC GTATAAATAT TTTGGCCCCA ATGGATGGAA AAAACGGGGG GGGGCCGGAA
1081 AGGAAAGGAT GC
//
Primer: IRES-F (Pr0036)
Region: IRES2,hKO,PRE
Sequence file: RDB14535_A7Ajc.seq check
>08350_14535_A7Aj_1_IRES-F_G07_19_ABI24.ab1
    1 GCGAAGCCTT TAAAATGGTG TTTAGTCGGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC
   61 ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACGGG GATCCCCACC
  121 ATGGTGAGCG TGATCAAGCC CGAGATGAAG ATGAAGTACT TCATGGACGG CAGCGTGAAC
  181 GGCCACGAGT TCACCGTGGA GGGCGAGGGC ACCGGCAAGC CCTACGAGGG CCACCAGGAG
  241 ATGACCCTGA GGGTGACAAT GGCCAAGGGC GGCCCCATGC CCTTCAGCTT CGACCTGGTG
  301 AGCCACACCT TCTGCTACGG CCACAGGCCC TTCACCAAGT ACCCCGAGGA GATCCCCGAC
  361 TACTTCAAGC AGGCCTTCCC CGAGGGCCTG AGCTGGGAGA GGAGCCTCCA GTTCGAGGAC
  421 GGCGGCTTCG CCGCCGTGAG CGCCCACATC AGCCTGAGGG GCAACTGCTT CGAGCACAAG
  481 AGCAAGTTCG TGGGCGTGAA CTTCCCCGCC GACGGCCCCG TGATGCAGAA CCAGAGCAGC
  541 GACTGGGAGC CCAGCACCGA GAAGATCACC ACCTGCGACG GCGTGCTGAA GGGCGACGTG
  601 ACCATGTACC TGAAGCTGGC CGGCGGCGGC AACCACAAGT GCCAGTTCAA GACCACCTAC
  661 AAGGCCGCCA AGAAGATCCT GAAGATGCCC CAGAGCCACT TCATCGGCCA CAGGCTGGTG
  721 AGGAAGACCG AGGGCAACAT CACCGAGCTG GTGGAGGACG CCGTGGCCCA CTGCTGACTG
  781 CAGGCTAGAG TTAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA
  841 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG
  901 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTTCC
  961 TTGTAATAAA TTCCTGGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGG TTGTCAGGCA
 1021 ACGGTGGCCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTT GGGGGCATTG
 1081 GCACACTGTC AGGCTCCCTT TCCGGACTTC GCTTTTCCCC CGTCCTAATT GTGGCCACCG
1141 GGCCGAACCA TCCATTTCG
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,5' LTR,HIV-1psi
Sequence file: RDB14535_A7Ajd.seq check
>08350_14535_A7Aj_1_CMV-Forward_B08_05_ABI24.ab1
    1 AGTCGCTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
  121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
  181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
  241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
  301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
  361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
  421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
  481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
  541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
  601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
  661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
  721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCTGA
  781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AAGGCAAAGA GAAGAGTGGT
  901 GCAGAGAGAA AAAAGAGCAG TTGGAATAGG AGCTTTTGTT CCTTGGGTTC TTGGGGAGCA
  961 GCAAGAGCAC TATGGGGCGC AGCGTTCATT GACGCTGACG GTACAGTAGA CATAATGTCT
 1021 GCTATAGTGC AGCAGCGACC ACTTGCCTGA CGCTATGAGG CCACAGCATC TGTGCCACCT
1081 CACAAG
//
Primer: f1ori_R3 (Pr0142)
Region: bGHpA,3' LTR
Sequence file: RDB14535_A7Aje.seq check
>08350_14535_A7Aj_1_f1ori_R3_E08_14_ABI24.ab1
    1 CGATGGACTG AGCCAGCTGG TTCTTTCCGC CTCAGAAGCC ATAGAGCCCA CCGCATCCCC
   61 AGCATGCCTG CTATTGTCTT CCCAATCCTC CCCCTTGCTG TCCTGCCCCA CCCCACCCCC
  121 CAGAATAGAA TGACACCTAC TCAGACAATG CGATGCAATT TCCTCATTTT ATTAGGAAAG
  181 GACAGTGGGA GTGGCACCTT CCAGGGTCAA GGAAGGCACG GGGGAGGGGC AAACAACAGA
  241 TGGCTGGCAA CTAGAAGGCA CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG
  301 AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA
  361 GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT
  421 TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC
  481 AGTCCGGATG CAGCTCTCGG GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT
  541 CTAATACTTC TCTCTCCGGG TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG
  601 TGTTCTCTCC TTCATTGGCT TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC
  661 ACCATCCAAA GGTCAGTGGA TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG
  721 GGAAGTAGCC TTGTGTGTGG TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT
  781 GAATTAGCCC TTTCCAGTCC CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC
  841 TTGTAAGTCA TTGGTCTTAA GGTACCTGAG TGTGACTGGA AAACCCACCT CTCTCCTCTG
  901 TGCTTCTAGC CAGGCACAAT CAGCATGGTA GCTGCTGTAT TGCTACTGTG ATGGCTCATG
  961 TTTTTTCTAG TCTCGACGGT ATCGAATGCC GGAGCCGCTA AACGGGAATT CCGAACTCGA
1021 TCTGAAGACT AGTAGACCGA AGCTCAGCGT CACAGTCCGG AGGAAGTC
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,Zeo,SV40pA
Sequence file: RDB14535_A7Ajf.seq check
>08350_14535_A7Aj_1_SV40pro_F_H08_23_ABI24.ab1
    1 CCTGTCCTTG CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA
  121 TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG
  181 TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG
  241 ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG
  301 GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG
  361 GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC
  421 ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG
  481 CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG
  541 GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
  601 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
  661 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
  721 ATCACAAATT TCACAAATAA AGCATTTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
  841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
  901 ACGAGCCGGA AGCATAAAGT GTAAAGCCCT GGGGGTGCCT AATGAGTGAG CTAACTCACA
  961 TTAAATTGCG TTTGCGCTCA CTGCCCGCTT TCCAGTCGGA AACCTGTCGT GCCAGCTGCA
 1021 TTAAATGAAA TCGGGCACGC GCGGGAGGAG GCGGGTTGCG TATGGGGCGC TCTTCGCTTC
 1081 CTCGATCCAC TTGAACTCGC TGCGCCTCGG ATCGGATTCC GGCCTTGCCG GGGAGAGCCG
1141 GGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Minagawa, A., Enhancing T Cell Receptor Stability in Rejuvenated iPSC-Derived T Cells Improves Their Use in Cancer Immunotherapy. Cell Stem Cell 23 (6): 850-858.e4 (2018). PMID 30449714. [link to Journal]