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CSIV-CMV-SV40LT-IRES2-Venus

Lentivirus vector plasmid of SV40 large T.

Catalog number RDB08327
Resource name CSIV-CMV-SV40LT-IRES2-Venus
Clone info. Lentivirus vector plasmid of SV40LT.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSIV-CMV-RfA-IRES2-Venus (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name SV40 large T antigen cDNA
Depositor|Developer Miyoshi, Hiroyuki |
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External Database
SV40 large T antigen

            Reference sequence
              
             
            Sequence RDB08327z.seq
            Remarks, protocol and/or map (pdf) RDB08327.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
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            手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08327 CSIV-CMV-SV40LT-IRES2-Venus DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSIV-CMV-SV40LT-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08327).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            Featured content

            Featured content Lentiviral plasmid list (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17949_B0Bdp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: LTAlarge_upR (Pr0144)
            Region: SV40LT,CMV pro2,CMV en2
            Sequence file: RDB17949_B0Bda.seq check
            >08327_17949_B0Bd_2_LTAlarge_upR_A07_01_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTGCN TCCNNGNAGC CTCCNAAGTC NGGTTGANGA GCATATTTTA
               61 CTCCATCTTC CATTTTCTTG TACAGAGTAT TCATTTTCTT CATTTTTTCT TCATCTCCTC
              121 CTTTATCAGG ATGAAACTCC TTGCATTTTT TTAAATATGC CTTTCTCATC AGAGGAATAT
              181 TCCCCCAGGC ACTCCTTTCA AGACCTAGAA GGTCCATTAG CTGCAAAGAT TCCTCTCTGT
              241 TTAAAACTTT ATCCATCTTG TCGAGACCGG TAGCGCTAGC GGATCTGACG GTTCACTAAA
              301 CCAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG
              361 GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA
              421 TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA
              481 TTGATGTACT GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC
              541 TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA
              601 CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG
              661 TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT
              721 ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA
              781 GGCGGGCCAT TTACCGTAAG TTATGTAACG CGGAACTCCN TATATGGGCT ATGAACTAAT
              841 GANNNNGTAA TTGATTACTA TTAATCGAAT TAATTCNAAG TGAATTTCTG CTGTCCCNGT
              901 AATAAACCCG AAAATTTTGA ATTTTTTGTA ATTTGNTTTT TGNAATTNNT TANTTTGNAT
            961 GTCNGNNGCT ANNATGNCNA CNNNNNNTTC CCCNNNNNNN NNN
            //
            Primer: IRES-R (Pr0037)
            Region: SV40LT
            Sequence file: RDB17949_B0Bdb.seq check
            >08327_17949_B0Bd_2_IRES-R_B07_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNGTTAGGG GGGGGGGNNG GNNANGGGCG GNNCCTCTAG
               61 AACTAGACTA GTTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG
              121 CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT CATGAACAGA CTGTGAGGAC
              181 TGAGGGGCCT GAAATGAGCC TTGGGACTGT GAATCAATGC CTGTTTCATG CCCTGAGTCT
              241 TCCATGTTCT TCTCCCCACC ATCTTCATTT TTATCAGCAT TTTCCTGGCT GTCTTCATCA
              301 TCATCATCAC TGTTTCTTAG CCAATCTAAA ACTCCAATTC CCATAGCCAC ATTAAACTTC
              361 ATTTTTTGAT ACACTGACAA ACTAAACTCT TTGTCCAATC TCTCTTTCCA CTCCACAATT
              421 CTGCTCTGAA TACTTTGAGC AAACTCAGCC ACAGGTCTGT ACCAAATTAA CATAAGAAGC
              481 AAAGCAATGC CACTTTGAAT TATTCTCTTT TCTAACAAAA ACTCACTGCG TTCCAGGCAA
              541 TGCTTTAAAT AATCTTTGGG CCTAAAATCT ATTTGTTTTA CAAATCTGGC CTGCAGTGTT
              601 TTAGGCACAC TGTACTCATT CATGGTGACT ATTCCAGGGG GAAATATTTG AGTTCTTTTA
              661 TTTAGGTGTT TCTTTTCTAA GTTTACCTTA ACACTGCCAT CCAAATAATC CCTTAAATTG
              721 TCCAGGTTAT TAATTCCCTG ACCTGAAGGC AAATCTCTGG ACTCCCCTCC AGTGCCCTTT
              781 ACATCCTCAA AAACTACTAA AAACTGNTCA ATAGCTACTC CTAGCTCAAA GTTCANCCTG
              841 TCNNAGGGCA AATTAACATT TAAAGCTTTC CCCCNACATA ATTCAAGCAA AGCAGCTGCT
              901 AATGTAGTTT TANCACTATC NNNTNNNCCT TTAAACAGCC AGTATCNTTT TTTNNNNNTG
              961 NTGTANNNCN NGCATTTTNA AAAGTCATNN NNNNNNGNAN TCCNTTTTNG GNNNNNNNNN
            1021 N
            //
            Primer: IRES-F (Pr0036)
            Region: IRES2,Venus
            Sequence file: RDB17949_B0Bdc.seq check
            >08327_17949_B0Bd_2_IRES-F_C07_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCGAGGTNNN NNAAACGTCT AGGCCCCCCG AACCACGGGG
               61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
              121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
              181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
              241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
              301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
              361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
              421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
              481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
              541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC
              601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG
              661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG
              721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGANTTCGTG
              781 ACCGCCGCCG GGNTCACTCT CGGCATGGAC GANCTGTACA AGTAAGAATT CAAGGCCTCT
              841 CGAGCCTCTA GAGTTAACAT CGAGGGANCA AGCTTATCGA TNATCNACCT CTGNATTACN
              901 AAATTTGTGA AAGANTGNNN GGTATTCTTA ACTATGTNGN NCCTTTTNCG CTANGNNGNA
              961 TACNCTGCTT TNATGNNNTT GNNTNANGCT NNNGNNNNCC GNATGGNNTN NNNTTTNNNN
            1021 NNNCNNNNNN NNANNNCNNN NNNNNNN
            //
            Primer: PRE_rev (Pr0250)
            Region: PRE,Venus
            Sequence file: RDB17949_B0Bdd.seq check
            >08327_17949_B0Bd_2_PRE_rev_D07_10_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNANNNNNNT AGTTAAGAAT ACCNGTCAAT CTTTCNCAAA
               61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGAGGC
              121 TCGAGAGGCC TTGAATTCTT ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC
              181 GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC
              241 GGACTGGTAG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT
              301 GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCCGCCGTCC TCGATGTTGT GGCGGATCTT
              361 GAAGTTGGCC TTGATGCCGT TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTGGCTGTT
              421 GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT
              481 CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA
              541 GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA
              601 CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCAGGCCGTA
              661 GCCCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATCAG
              721 CTTCAGGGTC AGCTTGCCGT ANGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT
              781 GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGNACAGCTC
              841 CTCGCCNTTG CTCACCANGG TTGTGGCCAT ATTATCATCG TGTTTTTCNA AGGAAANNCA
              901 CGTCCCCGNG GTTCGGGGGG GCCTANACGT TTTTTTTAAC CNNNNNNNAA CNCNTGTAAA
              961 GCNTGNGTAN CGANGNCNNN NNTCANNNNC NNTANNNNGG GGNNANNNTN NGGNNNNNCN
            1021 NNNNNNNNN
            //
            Primer: HIV-B_R (Pr0673)
            Region: HIV psi,5'LTR,CMV pro1,CMV en1
            Sequence file: RDB17949_B0Bde.seq check
            >08327_17949_B0Bd_2_HIV-B_R_E07_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN AANNTTTTGN NGTACTCNCC AGTCGCCGCC CCTCGCCTCT
               61 TGCCGTGCGC GCTTCNGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT
              121 CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT
              181 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT
              241 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG
              301 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC
              361 CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT
              421 TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG
              481 ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG
              541 CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA
              601 TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG
              661 GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA
              721 CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT
              781 TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG
              841 TTATGTAACG CGGAACTCCA TATATGGGCT ATGNACTAAT GANCCCNNNA TTGATTACTA
              901 TTAATAACTA GTCAATAATC AATGNCNACG NNNATATCNN GNCNNNACAT CGCGAANCAN
            961 CGCAAAACGN NNAANCNNNN NNANNNTNNN NNNNNNN
            //
            Primer: RRE_F1 (Pr0796)
            Region: RRE
            Sequence file: RDB17949_B0Bdf.seq check
            >08327_17949_B0Bd_2_RRE_F1_F07_16_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNANATTT GCTGANGGCT ATTGAGGCGC AACAGCATCT
               61 GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG
              121 ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC
              181 CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA
              241 CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT
              301 AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA
              361 ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT
              421 CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT
              481 GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG
              541 GGGACCCGAC AGGCCCGAAG GAATAGAAGA AGAANGTGGA GAGAGAGACA GANACAGATC
              601 CATTCGATTA GTGAACGGAT CTACAAATGG CAGTATTCAT CNNCAATTTT AAAAGAAAAG
              661 GGGGGANTGG GGGGTACAGT GCANGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC
              721 NAACTAAAGA NNTANNAAAA CNAATTACNA AAATTCAAAA TTTTCGGGNT TATTACNGGG
              781 ACAGCAGAAA NTCACTTTGA ATTNATTNNA NTAATAGTAA TCNATTACNG GGTCATTAGT
              841 TNATAGCCCN TATATGGANN NNCNCGNTAC NTNNNTTACN GNAAATGGNC CGNCNGGNNG
              901 ANCNCCCNNC GANCCCNNNC NNNNNNNGNN NNTAATGNNN TANGNNNCNN TANTANNGNC
            961 NANNNNGNNN NTTNNNNTNN NNNNNNNN
            //
            Primer: BGH_rev2 (Pr0606)
            Region: bGHp(A),3'LTR,loxP,PRE
            Sequence file: RDB17949_B0Bdg.seq check
            >08327_17949_B0Bd_2_BGH_rev2_G07_19_ABI24.ab1
                1 NNNNNNNNNN NNNNNGGNNG NNGGGCNNAC NACAGANGGC TGGCAACTAG AAGGCACAGT
               61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG
              121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
              181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
              241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGAATAACT TCGTATAATG
              301 TATGCTATAC GAAGTTATGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
              361 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
              421 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
              481 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
              541 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG
              601 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG
              661 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA
              721 GGCGAGCAGC CATGGAAAGG ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC
              781 CAACAGCCGA NCCCCTGTCC AGCAGCGGGC AAGGCAGGCG GCGATGANTT CCGCCGTGGC
              841 AATAGGGANG GGGAAAGCGA AAGTCCCGGA AAGGANCTGA NAGGNGGTGG CAATGCCCCA
              901 ANNAGTGGGG GGTTGNGTCA NCAAACNCNG NGCACACCAC NCCNCGTTGN CNNGANNNNN
            961 GGNNNNNNNN NN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40 pro_ori,Zeo,SV40p(A)
            Sequence file: RDB17949_B0Bdh.seq check
            >08327_17949_B0Bd_2_SV40pro_ori_F_H07_22_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGNCG CCNCTGCCTC TGAGCTATTC NNGAAGTAGT GAGGAGGCTT
               61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
              121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC
              181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC
              241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG
              301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG
              361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG
              421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG
              481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC
              541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC
              601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC
              661 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT
              721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
              781 CTGCATTCTA GTTGTGGTTT GTCCNAACTC ATCAATGTAT CTTATCATGT CTGTATACCG
              841 TCGACCTCTA GCTAGAGCTT NNCGTAATCA TGGTCATANC TGNTTNCTGT GTGAANNTGT
              901 TATCCGCTCN CNNTTNCACA CAACATACGA GCNNGNANNN ANNAAGNNNA AANCCNGGGG
            961 GNGCCNNNNG NNTGANCNNN NTCANNTTAN NNNCNNNNNN NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.11.23

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