Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSIV-CMV-SV40LT-IRES2-Venus

Lentivirus vector plasmid of SV40 large T.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17949_B0Bdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: LTAlarge_upR (Pr0144) Region: SV40LT,CMV pro2,CMV en2 Sequence file: RDB17949_B0Bda.seq
>08327_17949_B0Bd_2_LTAlarge_upR_A07_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNTGCN TCCNNGNAGC CTCCNAAGTC NGGTTGANGA GCATATTTTA    61 CTCCATCTTC CATTTTCTTG TACAGAGTAT TCATTTTCTT CATTTTTTCT TCATCTCCTC   121 CTTTATCAGG ATGAAACTCC TTGCATTTTT TTAAATATGC CTTTCTCATC AGAGGAATAT   181 TCCCCCAGGC ACTCCTTTCA AGACCTAGAA GGTCCATTAG CTGCAAAGAT TCCTCTCTGT   241 TTAAAACTTT ATCCATCTTG TCGAGACCGG TAGCGCTAGC GGATCTGACG GTTCACTAAA   301 CCAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG   361 GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA   421 TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA   481 TTGATGTACT GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC   541 TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA   601 CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG   661 TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT   721 ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA   781 GGCGGGCCAT TTACCGTAAG TTATGTAACG CGGAACTCCN TATATGGGCT ATGAACTAAT   841 GANNNNGTAA TTGATTACTA TTAATCGAAT TAATTCNAAG TGAATTTCTG CTGTCCCNGT   901 AATAAACCCG AAAATTTTGA ATTTTTTGTA ATTTGNTTTT TGNAATTNNT TANTTTGNAT 961 GTCNGNNGCT ANNATGNCNA CNNNNNNTTC CCCNNNNNNN NNN //
Primer: IRES-R (Pr0037) Region: SV40LT Sequence file: RDB17949_B0Bdb.seq
>08327_17949_B0Bd_2_IRES-R_B07_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGTTAGGG GGGGGGGNNG GNNANGGGCG GNNCCTCTAG    61 AACTAGACTA GTTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG   121 CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT CATGAACAGA CTGTGAGGAC   181 TGAGGGGCCT GAAATGAGCC TTGGGACTGT GAATCAATGC CTGTTTCATG CCCTGAGTCT   241 TCCATGTTCT TCTCCCCACC ATCTTCATTT TTATCAGCAT TTTCCTGGCT GTCTTCATCA   301 TCATCATCAC TGTTTCTTAG CCAATCTAAA ACTCCAATTC CCATAGCCAC ATTAAACTTC   361 ATTTTTTGAT ACACTGACAA ACTAAACTCT TTGTCCAATC TCTCTTTCCA CTCCACAATT   421 CTGCTCTGAA TACTTTGAGC AAACTCAGCC ACAGGTCTGT ACCAAATTAA CATAAGAAGC   481 AAAGCAATGC CACTTTGAAT TATTCTCTTT TCTAACAAAA ACTCACTGCG TTCCAGGCAA   541 TGCTTTAAAT AATCTTTGGG CCTAAAATCT ATTTGTTTTA CAAATCTGGC CTGCAGTGTT   601 TTAGGCACAC TGTACTCATT CATGGTGACT ATTCCAGGGG GAAATATTTG AGTTCTTTTA   661 TTTAGGTGTT TCTTTTCTAA GTTTACCTTA ACACTGCCAT CCAAATAATC CCTTAAATTG   721 TCCAGGTTAT TAATTCCCTG ACCTGAAGGC AAATCTCTGG ACTCCCCTCC AGTGCCCTTT   781 ACATCCTCAA AAACTACTAA AAACTGNTCA ATAGCTACTC CTAGCTCAAA GTTCANCCTG   841 TCNNAGGGCA AATTAACATT TAAAGCTTTC CCCCNACATA ATTCAAGCAA AGCAGCTGCT   901 AATGTAGTTT TANCACTATC NNNTNNNCCT TTAAACAGCC AGTATCNTTT TTTNNNNNTG   961 NTGTANNNCN NGCATTTTNA AAAGTCATNN NNNNNNGNAN TCCNTTTTNG GNNNNNNNNN 1021 N //
Primer: IRES-F (Pr0036) Region: IRES2,Venus Sequence file: RDB17949_B0Bdc.seq
>08327_17949_B0Bd_2_IRES-F_C07_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCGAGGTNNN NNAAACGTCT AGGCCCCCCG AACCACGGGG    61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC   121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC   181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG   241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG   301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC   361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC   421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG   481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC   541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC   601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG   661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG   721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGANTTCGTG   781 ACCGCCGCCG GGNTCACTCT CGGCATGGAC GANCTGTACA AGTAAGAATT CAAGGCCTCT   841 CGAGCCTCTA GAGTTAACAT CGAGGGANCA AGCTTATCGA TNATCNACCT CTGNATTACN   901 AAATTTGTGA AAGANTGNNN GGTATTCTTA ACTATGTNGN NCCTTTTNCG CTANGNNGNA   961 TACNCTGCTT TNATGNNNTT GNNTNANGCT NNNGNNNNCC GNATGGNNTN NNNTTTNNNN 1021 NNNCNNNNNN NNANNNCNNN NNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,Venus Sequence file: RDB17949_B0Bdd.seq
>08327_17949_B0Bd_2_PRE_rev_D07_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNANNNNNNT AGTTAAGAAT ACCNGTCAAT CTTTCNCAAA    61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGAGGC   121 TCGAGAGGCC TTGAATTCTT ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC   181 GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC   241 GGACTGGTAG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT   301 GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCCGCCGTCC TCGATGTTGT GGCGGATCTT   361 GAAGTTGGCC TTGATGCCGT TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTGGCTGTT   421 GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT   481 CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA   541 GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA   601 CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCAGGCCGTA   661 GCCCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATCAG   721 CTTCAGGGTC AGCTTGCCGT ANGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT   781 GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGNACAGCTC   841 CTCGCCNTTG CTCACCANGG TTGTGGCCAT ATTATCATCG TGTTTTTCNA AGGAAANNCA   901 CGTCCCCGNG GTTCGGGGGG GCCTANACGT TTTTTTTAAC CNNNNNNNAA CNCNTGTAAA   961 GCNTGNGTAN CGANGNCNNN NNTCANNNNC NNTANNNNGG GGNNANNNTN NGGNNNNNCN 1021 NNNNNNNNN //
Primer: HIV-B_R (Pr0673) Region: HIV psi,5'LTR,CMV pro1,CMV en1 Sequence file: RDB17949_B0Bde.seq
>08327_17949_B0Bd_2_HIV-B_R_E07_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN AANNTTTTGN NGTACTCNCC AGTCGCCGCC CCTCGCCTCT    61 TGCCGTGCGC GCTTCNGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT   121 CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT   181 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT   241 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG   301 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC   361 CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT   421 TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG   481 ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG   541 CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA   601 TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG   661 GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA   721 CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT   781 TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG   841 TTATGTAACG CGGAACTCCA TATATGGGCT ATGNACTAAT GANCCCNNNA TTGATTACTA   901 TTAATAACTA GTCAATAATC AATGNCNACG NNNATATCNN GNCNNNACAT CGCGAANCAN 961 CGCAAAACGN NNAANCNNNN NNANNNTNNN NNNNNNN //
Primer: RRE_F1 (Pr0796) Region: RRE Sequence file: RDB17949_B0Bdf.seq
>08327_17949_B0Bd_2_RRE_F1_F07_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNANATTT GCTGANGGCT ATTGAGGCGC AACAGCATCT    61 GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG   121 ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC   181 CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA   241 CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT   301 AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA   361 ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT   421 CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT   481 GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG   541 GGGACCCGAC AGGCCCGAAG GAATAGAAGA AGAANGTGGA GAGAGAGACA GANACAGATC   601 CATTCGATTA GTGAACGGAT CTACAAATGG CAGTATTCAT CNNCAATTTT AAAAGAAAAG   661 GGGGGANTGG GGGGTACAGT GCANGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC   721 NAACTAAAGA NNTANNAAAA CNAATTACNA AAATTCAAAA TTTTCGGGNT TATTACNGGG   781 ACAGCAGAAA NTCACTTTGA ATTNATTNNA NTAATAGTAA TCNATTACNG GGTCATTAGT   841 TNATAGCCCN TATATGGANN NNCNCGNTAC NTNNNTTACN GNAAATGGNC CGNCNGGNNG   901 ANCNCCCNNC GANCCCNNNC NNNNNNNGNN NNTAATGNNN TANGNNNCNN TANTANNGNC 961 NANNNNGNNN NTTNNNNTNN NNNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGHp(A),3'LTR,loxP,PRE Sequence file: RDB17949_B0Bdg.seq
>08327_17949_B0Bd_2_BGH_rev2_G07_19_ABI24.ab1     1 NNNNNNNNNN NNNNNGGNNG NNGGGCNNAC NACAGANGGC TGGCAACTAG AAGGCACAGT    61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG   121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA   181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC   241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGAATAACT TCGTATAATG   301 TATGCTATAC GAAGTTATGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC   361 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA   421 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC   481 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC   541 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG   601 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG   661 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA   721 GGCGAGCAGC CATGGAAAGG ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC   781 CAACAGCCGA NCCCCTGTCC AGCAGCGGGC AAGGCAGGCG GCGATGANTT CCGCCGTGGC   841 AATAGGGANG GGGAAAGCGA AAGTCCCGGA AAGGANCTGA NAGGNGGTGG CAATGCCCCA   901 ANNAGTGGGG GGTTGNGTCA NCAAACNCNG NGCACACCAC NCCNCGTTGN CNNGANNNNN 961 GGNNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,Zeo,SV40p(A) Sequence file: RDB17949_B0Bdh.seq
>08327_17949_B0Bd_2_SV40pro_ori_F_H07_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNCG CCNCTGCCTC TGAGCTATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC   121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC   181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC   241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG   301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG   361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG   421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG   481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC   541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC   601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC   661 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT   721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA   781 CTGCATTCTA GTTGTGGTTT GTCCNAACTC ATCAATGTAT CTTATCATGT CTGTATACCG   841 TCGACCTCTA GCTAGAGCTT NNCGTAATCA TGGTCATANC TGNTTNCTGT GTGAANNTGT   901 TATCCGCTCN CNNTTNCACA CAACATACGA GCNNGNANNN ANNAAGNNNA AANCCNGGGG 961 GNGCCNNNNG NNTGANCNNN NTCANNTTAN NNNCNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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