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CSIV-CMV-SV40LT-IRES2-Venus

Lentivirus vector construct of SV40 large T.

Catalog number RDB08327
Resource name CSIV-CMV-SV40LT-IRES2-Venus
Clone info. Lentivirus expression vector of SV40LT.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-CMV-RfA-IRES2-Venus (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name SV40 large T antigen cDNA
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering Please visit Ordering instruction. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08327 CSIV-CMV-SV40LT-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB08327z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB08327.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17949_B0Bdp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: LTAlarge_upR
Region: SV40LT,CMV pro2,CMV en2
Sequence file: RDB17949_B0Bda.seq check
>08327_17949_B0Bd_2_LTAlarge_upR_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTGCN TCCNNGNAGC CTCCNAAGTC NGGTTGANGA GCATATTTTA
   61 CTCCATCTTC CATTTTCTTG TACAGAGTAT TCATTTTCTT CATTTTTTCT TCATCTCCTC
  121 CTTTATCAGG ATGAAACTCC TTGCATTTTT TTAAATATGC CTTTCTCATC AGAGGAATAT
  181 TCCCCCAGGC ACTCCTTTCA AGACCTAGAA GGTCCATTAG CTGCAAAGAT TCCTCTCTGT
  241 TTAAAACTTT ATCCATCTTG TCGAGACCGG TAGCGCTAGC GGATCTGACG GTTCACTAAA
  301 CCAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG
  361 GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA
  421 TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA
  481 TTGATGTACT GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC
  541 TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA
  601 CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG
  661 TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT
  721 ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA
  781 GGCGGGCCAT TTACCGTAAG TTATGTAACG CGGAACTCCN TATATGGGCT ATGAACTAAT
  841 GANNNNGTAA TTGATTACTA TTAATCGAAT TAATTCNAAG TGAATTTCTG CTGTCCCNGT
  901 AATAAACCCG AAAATTTTGA ATTTTTTGTA ATTTGNTTTT TGNAATTNNT TANTTTGNAT
961 GTCNGNNGCT ANNATGNCNA CNNNNNNTTC CCCNNNNNNN NNN
//
Primer: IRES-R
Region: SV40LT
Sequence file: RDB17949_B0Bdb.seq check
>08327_17949_B0Bd_2_IRES-R_B07_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGTTAGGG GGGGGGGNNG GNNANGGGCG GNNCCTCTAG
   61 AACTAGACTA GTTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG
  121 CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT CATGAACAGA CTGTGAGGAC
  181 TGAGGGGCCT GAAATGAGCC TTGGGACTGT GAATCAATGC CTGTTTCATG CCCTGAGTCT
  241 TCCATGTTCT TCTCCCCACC ATCTTCATTT TTATCAGCAT TTTCCTGGCT GTCTTCATCA
  301 TCATCATCAC TGTTTCTTAG CCAATCTAAA ACTCCAATTC CCATAGCCAC ATTAAACTTC
  361 ATTTTTTGAT ACACTGACAA ACTAAACTCT TTGTCCAATC TCTCTTTCCA CTCCACAATT
  421 CTGCTCTGAA TACTTTGAGC AAACTCAGCC ACAGGTCTGT ACCAAATTAA CATAAGAAGC
  481 AAAGCAATGC CACTTTGAAT TATTCTCTTT TCTAACAAAA ACTCACTGCG TTCCAGGCAA
  541 TGCTTTAAAT AATCTTTGGG CCTAAAATCT ATTTGTTTTA CAAATCTGGC CTGCAGTGTT
  601 TTAGGCACAC TGTACTCATT CATGGTGACT ATTCCAGGGG GAAATATTTG AGTTCTTTTA
  661 TTTAGGTGTT TCTTTTCTAA GTTTACCTTA ACACTGCCAT CCAAATAATC CCTTAAATTG
  721 TCCAGGTTAT TAATTCCCTG ACCTGAAGGC AAATCTCTGG ACTCCCCTCC AGTGCCCTTT
  781 ACATCCTCAA AAACTACTAA AAACTGNTCA ATAGCTACTC CTAGCTCAAA GTTCANCCTG
  841 TCNNAGGGCA AATTAACATT TAAAGCTTTC CCCCNACATA ATTCAAGCAA AGCAGCTGCT
  901 AATGTAGTTT TANCACTATC NNNTNNNCCT TTAAACAGCC AGTATCNTTT TTTNNNNNTG
  961 NTGTANNNCN NGCATTTTNA AAAGTCATNN NNNNNNGNAN TCCNTTTTNG GNNNNNNNNN
1021 N
//
Primer: IRES-F
Region: IRES2,Venus
Sequence file: RDB17949_B0Bdc.seq check
>08327_17949_B0Bd_2_IRES-F_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCGAGGTNNN NNAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC
  601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG
  721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGANTTCGTG
  781 ACCGCCGCCG GGNTCACTCT CGGCATGGAC GANCTGTACA AGTAAGAATT CAAGGCCTCT
  841 CGAGCCTCTA GAGTTAACAT CGAGGGANCA AGCTTATCGA TNATCNACCT CTGNATTACN
  901 AAATTTGTGA AAGANTGNNN GGTATTCTTA ACTATGTNGN NCCTTTTNCG CTANGNNGNA
  961 TACNCTGCTT TNATGNNNTT GNNTNANGCT NNNGNNNNCC GNATGGNNTN NNNTTTNNNN
1021 NNNCNNNNNN NNANNNCNNN NNNNNNN
//
Primer: PRE_rev
Region: PRE,Venus
Sequence file: RDB17949_B0Bdd.seq check
>08327_17949_B0Bd_2_PRE_rev_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNANNNNNNT AGTTAAGAAT ACCNGTCAAT CTTTCNCAAA
   61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGAGGC
  121 TCGAGAGGCC TTGAATTCTT ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC
  181 GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC
  241 GGACTGGTAG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT
  301 GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCCGCCGTCC TCGATGTTGT GGCGGATCTT
  361 GAAGTTGGCC TTGATGCCGT TCTTCTGCTT GTCGGCGGTG ATATAGACGT TGTGGCTGTT
  421 GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT
  481 CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA
  541 GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA
  601 CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCAGGCCGTA
  661 GCCCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATCAG
  721 CTTCAGGGTC AGCTTGCCGT ANGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT
  781 GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGNACAGCTC
  841 CTCGCCNTTG CTCACCANGG TTGTGGCCAT ATTATCATCG TGTTTTTCNA AGGAAANNCA
  901 CGTCCCCGNG GTTCGGGGGG GCCTANACGT TTTTTTTAAC CNNNNNNNAA CNCNTGTAAA
  961 GCNTGNGTAN CGANGNCNNN NNTCANNNNC NNTANNNNGG GGNNANNNTN NGGNNNNNCN
1021 NNNNNNNNN
//
Primer: HIV-B_R
Region: HIV psi,5'LTR,CMV pro1,CMV en1
Sequence file: RDB17949_B0Bde.seq check
>08327_17949_B0Bd_2_HIV-B_R_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN AANNTTTTGN NGTACTCNCC AGTCGCCGCC CCTCGCCTCT
   61 TGCCGTGCGC GCTTCNGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT
  121 CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT
  181 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT
  241 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG
  301 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC
  361 CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT
  421 TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG
  481 ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG
  541 CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA
  601 TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG
  661 GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA
  721 CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT
  781 TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG
  841 TTATGTAACG CGGAACTCCA TATATGGGCT ATGNACTAAT GANCCCNNNA TTGATTACTA
  901 TTAATAACTA GTCAATAATC AATGNCNACG NNNATATCNN GNCNNNACAT CGCGAANCAN
961 CGCAAAACGN NNAANCNNNN NNANNNTNNN NNNNNNN
//
Primer: RRE_F1
Region: RRE
Sequence file: RDB17949_B0Bdf.seq check
>08327_17949_B0Bd_2_RRE_F1_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNANATTT GCTGANGGCT ATTGAGGCGC AACAGCATCT
   61 GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG
  121 ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC
  181 CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA
  241 CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT
  301 AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA
  361 ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT
  421 CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT
  481 GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG
  541 GGGACCCGAC AGGCCCGAAG GAATAGAAGA AGAANGTGGA GAGAGAGACA GANACAGATC
  601 CATTCGATTA GTGAACGGAT CTACAAATGG CAGTATTCAT CNNCAATTTT AAAAGAAAAG
  661 GGGGGANTGG GGGGTACAGT GCANGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC
  721 NAACTAAAGA NNTANNAAAA CNAATTACNA AAATTCAAAA TTTTCGGGNT TATTACNGGG
  781 ACAGCAGAAA NTCACTTTGA ATTNATTNNA NTAATAGTAA TCNATTACNG GGTCATTAGT
  841 TNATAGCCCN TATATGGANN NNCNCGNTAC NTNNNTTACN GNAAATGGNC CGNCNGGNNG
  901 ANCNCCCNNC GANCCCNNNC NNNNNNNGNN NNTAATGNNN TANGNNNCNN TANTANNGNC
961 NANNNNGNNN NTTNNNNTNN NNNNNNNN
//
Primer: BGH_rev2
Region: bGHp(A),3'LTR,loxP,PRE
Sequence file: RDB17949_B0Bdg.seq check
>08327_17949_B0Bd_2_BGH_rev2_G07_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNGGNNG NNGGGCNNAC NACAGANGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG
  121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
  181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
  241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGAATAACT TCGTATAATG
  301 TATGCTATAC GAAGTTATGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
  361 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
  421 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
  481 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
  541 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG
  601 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG
  661 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA
  721 GGCGAGCAGC CATGGAAAGG ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC
  781 CAACAGCCGA NCCCCTGTCC AGCAGCGGGC AAGGCAGGCG GCGATGANTT CCGCCGTGGC
  841 AATAGGGANG GGGAAAGCGA AAGTCCCGGA AAGGANCTGA NAGGNGGTGG CAATGCCCCA
  901 ANNAGTGGGG GGTTGNGTCA NCAAACNCNG NGCACACCAC NCCNCGTTGN CNNGANNNNN
961 GGNNNNNNNN NN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,Zeo,SV40p(A)
Sequence file: RDB17949_B0Bdh.seq check
>08327_17949_B0Bd_2_SV40pro_ori_F_H07_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNCG CCNCTGCCTC TGAGCTATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC
  181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC
  241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG
  301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG
  361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG
  421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG
  481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC
  541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC
  601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC
  661 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT
  721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
  781 CTGCATTCTA GTTGTGGTTT GTCCNAACTC ATCAATGTAT CTTATCATGT CTGTATACCG
  841 TCGACCTCTA GCTAGAGCTT NNCGTAATCA TGGTCATANC TGNTTNCTGT GTGAANNTGT
  901 TATCCGCTCN CNNTTNCACA CAACATACGA GCNNGNANNN ANNAAGNNNA AANCCNGGGG
961 GNGCCNNNNG NNTGANCNNN NTCANNTTAN NNNCNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.03.03

GNP_filter3_RDBDEP_html_200228.pl