Resource data sheet
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CSIV-CMV-SV40LT-IRES2-Venus

Lentivirus vector construct of SV40 large T.

Catalog number RDB08327
Resource name CSIV-CMV-SV40LT-IRES2-Venus
Clone info. Lentivirus expression vector of SV40LT.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-CMV-RfA-IRES2-Venus ()
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
Ordering Please visit Ordering instruction. 
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Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
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RDB08327 CSIV-CMV-SV40LT-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08327z.seq from Depositor
Electronic file PDF RDB08327.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08327_A6Chp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_F RDB08327_A6Cha.seq
>08327_08327_A6Ch_cPPT+CTS_F_G01_19.ab1
    1 AGGGAATGGG AATTAATTCG ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC
   61 ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA
  121 CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC
  181 TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA
  241 AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG
  301 GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT
  361 AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG
  421 GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG
  481 GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT
  541 GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTGGTTTAG TGAACCGTCA
  601 GATCCGCTAG CGCTACCGGT CTCGACAAGA TGGATAAAGT TTTAAACAGA GAGGAATCTT
  661 TGCAGCTAAT GGACCTTCTA GGTCTTGAAA GGAGTGCCTG GGGGAATATT CCTCTGATGA
  721 GAAAGGCATA TTTAAAAAAA TGCAAGGAGT TTCATCCTGA TAAAGGAGGA GATGAAGAAA
  781 AAATGAAGAA AATGAATACT CTGTACAAGA AAATGGAAGA TGGAGTAAAA TATGCTCATC
  841 AACCTGACTT TGGAGGCTTC TGGGATGCAA CTGAGATTCC AACCTATGGA ACTGATGAAT
  901 GGGAGCAGTG GTGGAATGCC TTTAATGAGG AAAACCTGTT TTGCTCAGAA GAAATGCCAT
  961 CTAGTGATGA TGAGGCTACT GCTGACTCTC AACATTCTAC TCCTCCAAAA AAGAAGAGAA
 1021 AGGTAGAGAC CCCAAGGACT TTCCCTTCAG AATTTGCTAA GTTTTTTGAG TCATGCTGGT
 1081 GTTTAGTAAT AGAACTCTTG CTTTGCTTTG CCTAATTTAC ACCACAAAGA AAGCTGCACT
1141 GCTTATTACA AGAAAAATTT ATTGGGCAAA AAAATAT
//
Primer: IRES-R RDB08327_A6Chb.seq
>08327_08327_A6Ch_IRES-R_H01_22.ab1
    1 GCTAGCGGCT TCGGCAGTAC GTTAGGGGGG GGGGAGGGAG AGGGGCGGAT CCTCTAGAAC
   61 TAGACTAGTT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA
  121 GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAT GAACAGACTG TGAGGACTGA
  181 GGGGCCTGAA ATGAGCCTTG GGACTGTGAA TCAATGCCTG TTTCATGCCC TGAGTCTTCC
  241 ATGTTCTTCT CCCCACCATC TTCATTTTTA TCAGCATTTT CCTGGCTGTC TTCATCATCA
  301 TCATCACTGT TTCTTAGCCA ATCTAAAACT CCAATTCCCA TAGCCACATT AAACTTCATT
  361 TTTTGATACA CTGACAAACT AAACTCTTTG TCCAATCTCT CTTTCCACTC CACAATTCTG
  421 CTCTGAATAC TTTGAGCAAA CTCAGCCACA GGTCTGTACC AAATTAACAT AAGAAGCAAA
  481 GCAATGCCAC TTTGAATTAT TCTCTTTTCT AACAAAAACT CACTGCGTTC CAGGCAATGC
  541 TTTAAATAAT CTTTGGGCCT AAAATCTATT TGTTTTACAA ATCTGGCCTG CAGTGTTTTA
  601 GGCACACTGT ACTCATTCAT GGTGACTATT CCAGGGGGAA ATATTTGAGT TCTTTTATTT
  661 AGGTGTTTCT TTTCTAAGTT TACCTTAACA CTGCCATCCA AATAATCCCT TAAATTGTCC
  721 AGGTTATTAA TTCCCTGACC TGAAGGCAAA TCTCTGGACT CCCCTCCAGT GCCCTTTACA
  781 TCCTCAAAAA CTACTAAAAA CTGGTCAATA GCTACTCCTA GCTCAAAGTT CAGCCTGTCC
  841 AAAGGGCAAA TTAACATTTA AAGCTTTCCC CCCACATAAT TCAAGCAAAG CAGCTGCTAA
  901 TGTAGTTTTA CCACTATCAA TTGGTCCTTT AAACAGCCAG TATCTTTTTT TAGGAATGTT
  961 GTACACCATG CATTTTAAAA AGTCATACAC CACTGAATCA TTTTGGGCAC AAACAGTGTA
 1021 GCCAGCACTC AGCCATCATT CTTCTTATGT CAGCAGAGCT GTAGACAAAC ATTATATCAT
 1081 TCTATCGAAA GATCATAATC TGTTGGTACA TGGTCCTAGT TAATGTAGCT ATCAGCCCGC
1141 TTTTTAAGCC TA
//
Primer: IRES-F RDB08327_A6Chc.seq
>08327_08327_A6Ch_IRES-F_A02_02.ab1
    1 CCATCCCTTA CTGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA
   61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGTG AGCAAGGGCG
  121 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
  181 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA
  241 AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG
  301 GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
  361 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA
  421 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC
  481 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT
  541 ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGCCAACT
  601 TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC TACCAGCAGA
  661 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT
  721 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA
  781 CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTAAGAATTC AAGGCCTCTC
  841 GAGCCTCTAG AGTTAACATC GAGGGATCAA GCTTATCGAT AATCAACCTC TGGATTACAA
  901 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA
  961 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTTCTCCT
 1021 CCTTGTATAA ATCCTGGATG CTGTCTCTTT ATGAGAGTGT GGCCCGTTGT CAGCCACGTT
 1081 GCGTGATGTG CCACTGTGAT TGCTGACGCT ACCCCACTGT ATGGGGCCAT GCCACCACCC
1141 TGTTCAAGCC TCCCAT
//
Primer: HIVgp_R1 RDB08327_A6Chd.seq
>08327_08327_A6Ch_HIVgp_R1_B02_05.ab1
    1 CGGGCCTTCT ATTCCCACTG CTCTTTTTTC TCTCTGCACC ACTCTTCTCT TTGCCTTGGT
   61 GGGTGCTACT CCTAATGGTT CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA
  121 ATTGTCCCTC ATATCTCCTC CTCCAGGTCT GAAGATCAGC GGCCGGCCGC TTGCTGTGCG
  181 GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT
  241 ATCTCTATCC TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA TGATCTAAGT
  301 TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT
  361 GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT
  421 ATATGTTTTA ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC
  481 GATCTAATTC TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT
  541 CCGCTAGTCA AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC
  601 GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG
  661 TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC
  721 TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC
  781 TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT
  841 GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC CTCCCACCGT
  901 ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT TGGAAAGTCC
  961 CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT
 1021 CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAACGCA TCACCATGGT
 1081 AATAGCGATG ACTTAATACG TAGATGTACT GCCAAGTAGA AAGTTCCATA GGTCATGTAC
1141 TGGGGCATAA ATGCCCAAGG CCGGGGCCCA A
//
Primer: BGH_rev2 RDB08327_A6Che.seq
>08327_08327_A6Ch_BGH_rev2_C02_08.ab1
    1 CCAGTTGGCG GTCATGTACG CGCGTGGGGA GGGCAAGCAA GCAGATGGCT GGCAACTAGA
   61 AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG
  121 ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA
  181 CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG
  241 AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGAATAACTT
  301 CGTATAATGT ATGCTATACG AAGTTATGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC
  361 TTCCAGTCCC CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT
  421 TGGTCTTAAA GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC
  481 TAGCCAGGCA CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT
  541 TTCTAGGTCT CGACGGTATC GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG
  601 AGGGCGAAGG CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG
  661 GTCCGCTGGA TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT
  721 GGCAACACAG GCGAGCAGCC ATGGAAAGGA CGTCAGCTTC CCCGACAACA CCACGGAATT
  781 GTCAGTGCCC AACAGCCGAG CCCCTGTCCA GCAGCGGGCA AGGCAGGCGG CGATGAGTTC
  841 CGCCGTGGCA ATAGGGAGGG GGAAAGCGAA AGTCCCGGAA AGGAGCTGAC AGGTGGTGGC
  901 AATGCCCCAA CCAGTGGGGG TTGCGTCAGC AACACAGTGC ACACCACGCC ACGTTGCCTG
  961 ACAACGGGCC ACAACTCCTC ATAAGAGACA GCACCCAGGA TTTATACAAG GAGGAGAAAA
 1021 TGAAGCCATA CGGGAAGCAA TAGCATGATA CAAGGCATTA AGCAGCGTAT CCACATAGCG
 1081 GTAAAAGGAG CACATAGTTA GAATACCCAG TTCAATCTTT CACAAATTTG GTATTCAGAG
1141 GGTGAATTAT CGATAAGGCT TTGGAATCCC TCTTCGAAG
//
Primer: SV40pro_F RDB08327_A6Chf.seq
>08327_08327_A6Ch_SV40pro_F_D02_11.ab1
    1 CTGCTGTTTT CGGAGACATT CCAGAGGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
   61 TGCAAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAGC ACGTGTTGAC
  121 AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG ACAAGGTGAG GAACTAAACC
  181 ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC GCGACGTCGC CGGAGCGGTC
  241 GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG TGGAGGACGA CTTCGCCGGT
  301 GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC AGGACCAGGT GGTGCCGGAC
  361 AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC TGTACGCCGA GTGGTCGGAG
  421 GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA TGACCGAGAT CGGCGAGCAG
  481 CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC
  541 GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG
  601 TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC
  661 ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA
  721 AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT
  781 TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC
  841 TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA
  901 CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA
  961 CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTTCCAG TCGGGAAAAC CTGTCGTGCC
 1021 AGCTGCATTA ATGAAATCGG GCCAAACGCG CGGGAAGAGG CGGGTTGCGT ATTGGGGCGC
 1081 TTCTTTCCGG CTTCTCGCTC AACTGAACTT CGCTGCGGCC TCGTCGTTCG CCTGCGGCGA
 1141 GACCGGCTAT CCAGCTTCAC TTCAAAGTCG TATTACCGGC TATTTCCCAC GGAGAAACTG
1201 AT
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.10.09

GNP_filter3_RDBDEP_html_180823.pl