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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSIV-CMV-MCS-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.

Catalog number RDB08257
Resource name CSIV-CMV-MCS-IRES2-Venus
Clone info. Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
            Ordering Please visit Ordering instruction.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            Please contact us. We will provide you with appropriate documents.
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            付加的提供条件 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            宮脇敦史博士による提供承諾書 [Word]
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            クローンに使用している蛍光タ
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08257 CSIV-CMV-MCS-IRES2-Venus DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB08257z.seq
            Remarks, protocol and/or map (pdf) RDB08257.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17906_B0A9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: cPPT+CTS_F
            Region: CMV en,CMV pro,IRES2
            Sequence file: RDB17906_B0A9a.seq check
            >08257_17906_B0A9_3_cPPT+CTS_F_A05_01_ABI08.ab1
                1 NNNNNNNNNN NNTCNATNNN NAGTAATCNA TTACGGGGTC NTTAGTTCNT AGCCCATATA
               61 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC
              121 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC
              181 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT
              241 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
              301 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA
              361 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG
              421 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
              481 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG
              541 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC
              601 GCTAGCGCTA CCGGTCTCGA GAATTCTGCA GCGGCCGCGG ATCCGCCCCT CTCCCTCCCC
              661 CCCCCCTAAC GTTACTGGCC GAAGCCGCTT GGAATAAGGC CGGTGTGCGT TTGTCTATAT
              721 GTTATTTTCC ACCATATTGC CGTCTTTTGG CAATGTGAGG GCCCGGAAAC CTGGCCCTGT
              781 CTTCTTGACG AGCATTCCTA GGGGTCTTTC NNCTCTCGCC NAAGGAATGC AAGGTCTGTT
              841 GAATGTCGTG AANGAANCAG TTCCTCTGGA AGCTTNTTGA ANANAAACNA CGTCTGTANC
              901 GANCCTTTGC ANGNAGCGNA ACCCCCCANN NNGCGNNAGG TGCCTNTGCN GNNNAAAGNN
            961 NCGNGNANNA GANACNCNNG NNANNNNNNN NNN
            //
            Primer: PRE_rev
            Region: WPRE,Venus
            Sequence file: RDB17906_B0A9b.seq check
            >08257_17906_B0A9_3_PRE_rev_B05_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNCNNN NNNGGAGCNA CATAGTTAAG AATACCAGTC AATCTTTCNC
               61 AAATTTTGTA ATCCAGAGGT TGATTATCGA TAAGCTTGAT CCCTCGATGT TAACTCTAGA
              121 GGCTCGAGAG GCCTTGAATT CTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC
              181 GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG
              241 GGCGGACTGG TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG
              301 GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT
              361 CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT
              421 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC
              481 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT
              541 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC
              601 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC
              661 GTAGCCCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT
              721 CAGCTTCAGG GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA
              781 CTTGTGGNCG TTTACGTCGC CGTCCAGCTC GACCAGGANG GGCACCACCC CGGTGAACAG
              841 CTCCTCGCCC TTGCTCNCCA TGGTTGTGGN NATATTATCA TCGNGNTTTT CAAAGGAAAA
              901 NNNCGTCCCC GNNGTNNGGG GGGGCCTANN NNTTTTTTTT NACCTCGANN NAACNCNTGN
              961 NANGCNTGTG TANCNGAGGC CCNNGATCAN ATNCNNTANN NNGGGGGNAN CNNTNNNGGN
            1021 NTNNNNNNNN NN
            //
            Primer: IRES-F
            Region: IRES2,Venus
            Sequence file: RDB17906_B0A9c.seq check
            >08257_17906_B0A9_3_IRES-F_F05_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCGANGTTNN NAAAACGTCT AGGCCCCCCG AACCACGGGG
               61 NCGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
              121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
              181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
              241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
              301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
              361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
              421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
              481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
              541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC
              601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG
              661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG
              721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
              781 ACCGCCGCCG GGATCACTCT CGGCATGGNC GANCTGTACN AGTAAGAATT CNAGGCCTCT
              841 CGANCCTCTA NAGTTNACAT CGNNGGGATC ANGCTTATCG ATAATCNANC TCTGGANTAC
              901 AAAATTTGTG AAANANTGNN NGGNATTCTT NNNTATGTTG CTCCTTTTAC GCTATGNGNA
              961 NACGCTGCTT TNATGCCTTN NNATCNNGCN NTTGNNNNCC NNATGGNTTT NNNTTNNNNN
            1021 NNCNNNNNNN NANNNNNNNN NN
            //
            Primer: HIV-B_R
            Region: HIV-1 psi,5'LTR,CMVpro,CMV en
            Sequence file: RDB17906_B0A9d.seq check
            >08257_17906_B0A9_3_HIV-B_R_C05_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTTT GNNGTACTCN CCNGTCGCCG CCCCTCGCCT
               61 CTTGCCGTGC GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT
              121 TTCGCTTTCA AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG
              181 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
              241 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
              301 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAACGCGCT GCTTATATAG
              361 ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT GTTACGACAT
              421 TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC AATGGGGTGG
              481 AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA CTGCCAAAAC
              541 CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC
              601 CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA
              661 GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA
              721 TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG TTACTATGGG AACATACGTC
              781 ATTATTGACG TCNATGNGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA
              841 AGTTATGTAA CGCGGAACTN CNTATATGGG CTATGAACTA ATGANCCCGT NATTGATTAC
              901 TATTAATAAC TAGTCNATAA TCANTGTCAA CGCGTATATC TGGNCNNNNN NTCNNGAANC
              961 ANCNNNAAAN GCCTAANNNN NAGCANANTC NNNNTGNNAN TNNNCNNNNN ANNNNTNNNN
            1021 NNNNNN
            //
            Primer: cPPT_CTS-E_R
            Region: cPPT_CTS,RRE
            Sequence file: RDB17906_B0A9e.seq check
            >08257_17906_B0A9_3_cPPT_CTS-E_R_E05_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNTTT TNNNNNCTTN AGTTTGTATG TCTGTTGCTA TTATGTCTAC
               61 TATTCTTTCC CCTNNNNNNN NCCCCCCAAT CCCCCCTTTT CTTTTAAAAT TGTGGATGAA
              121 TACTGCCATT TGTAGATCCG TTCACTAATC GAATGGATCT GTCTCTGTCT CTCTCTCCAC
              181 CTTCTTCTTC TATTCCTTCG GGCCTGTCGG GTCCCCTCGG GGTTGGGAGG TGGGTCTGAA
              241 ACGATAATGG TGAATATCCC TGCCTAACTC TATTCACTAT AGAAAGTACA GCAAAAACTA
              301 TTCTTAAACC TACCAAGCCT CCTACTATCA TTATGAATAA TTTTATATAC CACAGCCAAT
              361 TTGTTATGTT AAACCAATTC CACAAACTTG CCCATTTATC TAATTCCAAT AATTCTTGTT
              421 CATTCTTTTC TTGCTGGTTT TGCGATTCTT CAATTAAGGA GTGTATTAAG CTTGTGTAAT
              481 TGTTAATTTC TCTGTCCCAC TCCATCCAGG TCGTGTGATT CCAAATCTGT TCCAGAGATT
              541 TATTACTCCA ACTAGCATTC CAAGGCACAG CAGTGGTGCA AATGAGTTTT CCAGAGCAAC
              601 CCCAAATCCC CAGGAGCTGT TGATCCTTTA GGTATCTTTC CACAGCCAGG ATTCTTGCCT
              661 GGAGCTGCTT GATGCCCCAG ACTGTGAGTT GCAACAGATG CTGTTGCGCC TCAATAGCCC
              721 TCAGCAAATT GTTCTGCTGC TGCACTATAC CAGACAATAA TTGTCTGGNC TGTACCGTCA
              781 GCGTCATTGA CGCTGCGCCC ATAGTGCTTC CTGCTGCTCC CAAGAACCCA ANGAACNAAG
              841 CTCCTATTCC CACTGCTCTT TTTTCTCTCT GCACCACTCT TCTCTTTGCC TTGNNGGGTG
              901 CTACTCCNAA NGNNTCNATT TTNACNACTT TATANTTATA TAATTCACTT CTCCNNNTGT
              961 CCNNNATATC TCCNCCTCCA NGNNTGANNA NCANCNGNNN NGNNNNNNGN NNNGCNNNNN
            1021 NNNNN
            //
            Primer: BGH_rev2
            Region: bGHp(A),3'LTR,loxP,WPRE
            Sequence file: RDB17906_B0A9f.seq check
            >08257_17906_B0A9_3_BGH_rev2_H07_22_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGGG CNNACAACAG NTGGCTGGCA ACTAGAAGGC
               61 NCAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
              121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
              181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
              241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAA TAACTTCGTA
              301 TAATGTATGC TATACGAAGT TATGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC
              361 AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT
              421 CTTAAAGGTA CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC
              481 CAGGCACAAT CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT
              541 AGGTCTCGAC GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG
              601 CGAANGCGAA GANGCGGAAG AGGCCGCANA GCCGGCAGCA NGCCGCGGGA ANGAANGTCC
              661 GCTGGATTGA GGGCCGAANG GACGTAGCAG AANGACGTCC CGCGCAGAAT CCAGGTGGCA
              721 ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCNCG ACNACACCAC GGAATTGTCN
              781 GTGCCCAACA GNCGANCCCC TGTCCNGCAG CGGGCAAGGC AGGCNGCGAT GANTNCCGCC
              841 NNNGCNNTAN GGANGGGGAA AGCGAAAGNN CCNNNAANGA NCTGANNNGN GNNNGCNNTG
            901 NCNNNNNNNN NN
            //
            Primer: SV40pro_ori_F
            Region: SV40pro_ori,EM7 pro,Zeo
            Sequence file: RDB17906_B0A9g.seq check
            >08257_17906_B0A9_3_SV40pro_ori_F_G05_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNGNC GCCTCTGCCT CTGAGCTATT CCNGAAGTAG TGAGGAGGCT
               61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
              121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
              181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
              241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
              301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
              361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
              421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
              481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
              541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC
              601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT
              661 CCTCCAGCGC GGGGNTCTCA TGCTGGAGTT CTTCGCCNAC CCCAACTTGT TTATTGCAGC
              721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
              781 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC
              841 GTCNANCTCT ANCTAGAGCT TGNCGTAATC ATGGTCATAN CTGTTTCCTG NGTGAAATTG
              901 TTATCCGCTC NCAATNNCNC ACAACATACN ANCNGGAANN ATAAAGNGNA AAGCCNGGGG
              961 NGNCNAATGA NNNANCNNNN NNNCNNNNAT TNNNNNGCGN NNNCNNCCNN NNTTNNNNNC
            1021 NGGNNAANCN NNNNNNNNCN NNNNNNNNTN NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Ichikawa, D., Integrated diagnosis based on transcriptome analysis in suspected pediatric sarcomas. NPJ Genom. Med. 6(1): 49 (2021). PMID 34131151.
            user_report Hirota, A., The nucleosome remodeling and deacetylase complex protein CHD4 regulates neural differentiation of mouse embryonic stem cells by down-regulating p53. J. Biol. Chem. 294 (1): 195-209 (2019). PMID 30409903.
            user_report Fujimoto, S., KLF4 prevents epithelial to mesenchymal transition in human corneal epithelial cells via endogenous TGF-beta2 suppression. Regen. Ther. 11: 249-257 (2019). PMID 31538102.
            user_report Suzuki, K., MEF2D-BCL9 Fusion Gene Is Associated With High-Risk Acute B-Cell Precursor Lymphoblastic Leukemia in Adolescents. J. Clin. Oncol. 34 (28): 3451-3459 (2016). PMID 27507882.
            user_report Yamaguchi, S., Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors. Cancer Sci. 105 (4): 402-408 (2014). PMID 24521492.

            2022.05.17

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