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CSIV-CMV-MCS-IRES2-Venus (#RDB08257)

Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.

Clone info. Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB08257z.seq
Remarks, protocol and/or map (pdf) RDB08257.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08257 CSIV-CMV-MCS-IRES2-Venus Please contact us. JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSIV-CMV-MCS-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08257).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17906_B0A9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_F (Pr0161)
Region: CMV en,CMV pro,IRES2
Sequence file: RDB17906_B0A9a.seq check
>08257_17906_B0A9_3_cPPT+CTS_F_A05_01_ABI08.ab1
    1 NNNNNNNNNN NNTCNATNNN NAGTAATCNA TTACGGGGTC NTTAGTTCNT AGCCCATATA
   61 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC
  121 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC
  181 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT
  241 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
  301 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA
  361 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG
  421 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
  481 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG
  541 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC
  601 GCTAGCGCTA CCGGTCTCGA GAATTCTGCA GCGGCCGCGG ATCCGCCCCT CTCCCTCCCC
  661 CCCCCCTAAC GTTACTGGCC GAAGCCGCTT GGAATAAGGC CGGTGTGCGT TTGTCTATAT
  721 GTTATTTTCC ACCATATTGC CGTCTTTTGG CAATGTGAGG GCCCGGAAAC CTGGCCCTGT
  781 CTTCTTGACG AGCATTCCTA GGGGTCTTTC NNCTCTCGCC NAAGGAATGC AAGGTCTGTT
  841 GAATGTCGTG AANGAANCAG TTCCTCTGGA AGCTTNTTGA ANANAAACNA CGTCTGTANC
  901 GANCCTTTGC ANGNAGCGNA ACCCCCCANN NNGCGNNAGG TGCCTNTGCN GNNNAAAGNN
961 NCGNGNANNA GANACNCNNG NNANNNNNNN NNN
//
Primer: PRE_rev (Pr0250)
Region: WPRE,Venus
Sequence file: RDB17906_B0A9b.seq check
>08257_17906_B0A9_3_PRE_rev_B05_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCNNN NNNGGAGCNA CATAGTTAAG AATACCAGTC AATCTTTCNC
   61 AAATTTTGTA ATCCAGAGGT TGATTATCGA TAAGCTTGAT CCCTCGATGT TAACTCTAGA
  121 GGCTCGAGAG GCCTTGAATT CTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC
  181 GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG
  241 GGCGGACTGG TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG
  301 GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT TGTGGCGGAT
  361 CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA CGTTGTGGCT
  421 GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC
  481 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT
  541 GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC
  601 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC ACTGCAGGCC
  661 GTAGCCCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT
  721 CAGCTTCAGG GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA
  781 CTTGTGGNCG TTTACGTCGC CGTCCAGCTC GACCAGGANG GGCACCACCC CGGTGAACAG
  841 CTCCTCGCCC TTGCTCNCCA TGGTTGTGGN NATATTATCA TCGNGNTTTT CAAAGGAAAA
  901 NNNCGTCCCC GNNGTNNGGG GGGGCCTANN NNTTTTTTTT NACCTCGANN NAACNCNTGN
  961 NANGCNTGTG TANCNGAGGC CCNNGATCAN ATNCNNTANN NNGGGGGNAN CNNTNNNGGN
1021 NTNNNNNNNN NN
//
Primer: IRES-F (Pr0036)
Region: IRES2,Venus
Sequence file: RDB17906_B0A9c.seq check
>08257_17906_B0A9_3_IRES-F_F05_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCGANGTTNN NAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 NCGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC
  601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG
  721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
  781 ACCGCCGCCG GGATCACTCT CGGCATGGNC GANCTGTACN AGTAAGAATT CNAGGCCTCT
  841 CGANCCTCTA NAGTTNACAT CGNNGGGATC ANGCTTATCG ATAATCNANC TCTGGANTAC
  901 AAAATTTGTG AAANANTGNN NGGNATTCTT NNNTATGTTG CTCCTTTTAC GCTATGNGNA
  961 NACGCTGCTT TNATGCCTTN NNATCNNGCN NTTGNNNNCC NNATGGNTTT NNNTTNNNNN
1021 NNCNNNNNNN NANNNNNNNN NN
//
Primer: HIV-B_R (Pr0673)
Region: HIV-1 psi,5'LTR,CMVpro,CMV en
Sequence file: RDB17906_B0A9d.seq check
>08257_17906_B0A9_3_HIV-B_R_C05_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTTT GNNGTACTCN CCNGTCGCCG CCCCTCGCCT
   61 CTTGCCGTGC GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT
  121 TTCGCTTTCA AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG
  181 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
  241 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
  301 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAACGCGCT GCTTATATAG
  361 ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT GTTACGACAT
  421 TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC AATGGGGTGG
  481 AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA CTGCCAAAAC
  541 CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC
  601 CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA
  661 GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA
  721 TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG TTACTATGGG AACATACGTC
  781 ATTATTGACG TCNATGNGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA
  841 AGTTATGTAA CGCGGAACTN CNTATATGGG CTATGAACTA ATGANCCCGT NATTGATTAC
  901 TATTAATAAC TAGTCNATAA TCANTGTCAA CGCGTATATC TGGNCNNNNN NTCNNGAANC
  961 ANCNNNAAAN GCCTAANNNN NAGCANANTC NNNNTGNNAN TNNNCNNNNN ANNNNTNNNN
1021 NNNNNN
//
Primer: cPPT_CTS-E_R (Pr0725)
Region: cPPT_CTS,RRE
Sequence file: RDB17906_B0A9e.seq check
>08257_17906_B0A9_3_cPPT_CTS-E_R_E05_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNTTT TNNNNNCTTN AGTTTGTATG TCTGTTGCTA TTATGTCTAC
   61 TATTCTTTCC CCTNNNNNNN NCCCCCCAAT CCCCCCTTTT CTTTTAAAAT TGTGGATGAA
  121 TACTGCCATT TGTAGATCCG TTCACTAATC GAATGGATCT GTCTCTGTCT CTCTCTCCAC
  181 CTTCTTCTTC TATTCCTTCG GGCCTGTCGG GTCCCCTCGG GGTTGGGAGG TGGGTCTGAA
  241 ACGATAATGG TGAATATCCC TGCCTAACTC TATTCACTAT AGAAAGTACA GCAAAAACTA
  301 TTCTTAAACC TACCAAGCCT CCTACTATCA TTATGAATAA TTTTATATAC CACAGCCAAT
  361 TTGTTATGTT AAACCAATTC CACAAACTTG CCCATTTATC TAATTCCAAT AATTCTTGTT
  421 CATTCTTTTC TTGCTGGTTT TGCGATTCTT CAATTAAGGA GTGTATTAAG CTTGTGTAAT
  481 TGTTAATTTC TCTGTCCCAC TCCATCCAGG TCGTGTGATT CCAAATCTGT TCCAGAGATT
  541 TATTACTCCA ACTAGCATTC CAAGGCACAG CAGTGGTGCA AATGAGTTTT CCAGAGCAAC
  601 CCCAAATCCC CAGGAGCTGT TGATCCTTTA GGTATCTTTC CACAGCCAGG ATTCTTGCCT
  661 GGAGCTGCTT GATGCCCCAG ACTGTGAGTT GCAACAGATG CTGTTGCGCC TCAATAGCCC
  721 TCAGCAAATT GTTCTGCTGC TGCACTATAC CAGACAATAA TTGTCTGGNC TGTACCGTCA
  781 GCGTCATTGA CGCTGCGCCC ATAGTGCTTC CTGCTGCTCC CAAGAACCCA ANGAACNAAG
  841 CTCCTATTCC CACTGCTCTT TTTTCTCTCT GCACCACTCT TCTCTTTGCC TTGNNGGGTG
  901 CTACTCCNAA NGNNTCNATT TTNACNACTT TATANTTATA TAATTCACTT CTCCNNNTGT
  961 CCNNNATATC TCCNCCTCCA NGNNTGANNA NCANCNGNNN NGNNNNNNGN NNNGCNNNNN
1021 NNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGHp(A),3'LTR,loxP,WPRE
Sequence file: RDB17906_B0A9f.seq check
>08257_17906_B0A9_3_BGH_rev2_H07_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGGG CNNACAACAG NTGGCTGGCA ACTAGAAGGC
   61 NCAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
  121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
  181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
  241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAA TAACTTCGTA
  301 TAATGTATGC TATACGAAGT TATGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC
  361 AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT
  421 CTTAAAGGTA CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC
  481 CAGGCACAAT CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT
  541 AGGTCTCGAC GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG
  601 CGAANGCGAA GANGCGGAAG AGGCCGCANA GCCGGCAGCA NGCCGCGGGA ANGAANGTCC
  661 GCTGGATTGA GGGCCGAANG GACGTAGCAG AANGACGTCC CGCGCAGAAT CCAGGTGGCA
  721 ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCNCG ACNACACCAC GGAATTGTCN
  781 GTGCCCAACA GNCGANCCCC TGTCCNGCAG CGGGCAAGGC AGGCNGCGAT GANTNCCGCC
  841 NNNGCNNTAN GGANGGGGAA AGCGAAAGNN CCNNNAANGA NCTGANNNGN GNNNGCNNTG
901 NCNNNNNNNN NN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori,EM7 pro,Zeo
Sequence file: RDB17906_B0A9g.seq check
>08257_17906_B0A9_3_SV40pro_ori_F_G05_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGNC GCCTCTGCCT CTGAGCTATT CCNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
  121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
  181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
  241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
  301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
  361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
  421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
  481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
  541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC
  601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT
  661 CCTCCAGCGC GGGGNTCTCA TGCTGGAGTT CTTCGCCNAC CCCAACTTGT TTATTGCAGC
  721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  781 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC
  841 GTCNANCTCT ANCTAGAGCT TGNCGTAATC ATGGTCATAN CTGTTTCCTG NGTGAAATTG
  901 TTATCCGCTC NCAATNNCNC ACAACATACN ANCNGGAANN ATAAAGNGNA AAGCCNGGGG
  961 NGNCNAATGA NNNANCNNNN NNNCNNNNAT TNNNNNGCGN NNNCNNCCNN NNTTNNNNNC
1021 NGGNNAANCN NNNNNNNNCN NNNNNNNNTN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Ichikawa, D., Integrated diagnosis based on transcriptome analysis in suspected pediatric sarcomas. NPJ Genom. Med. 6(1): 49 (2021). PMID 34131151. [link to RRC of NBRP]
user_report Hirota, A., The nucleosome remodeling and deacetylase complex protein CHD4 regulates neural differentiation of mouse embryonic stem cells by down-regulating p53. J. Biol. Chem. 294 (1): 195-209 (2019). PMID 30409903. [link to RRC of NBRP]
user_report Fujimoto, S., KLF4 prevents epithelial to mesenchymal transition in human corneal epithelial cells via endogenous TGF-beta2 suppression. Regen. Ther. 11: 249-257 (2019). PMID 31538102. [link to RRC of NBRP]
user_report Suzuki, K., MEF2D-BCL9 Fusion Gene Is Associated With High-Risk Acute B-Cell Precursor Lymphoblastic Leukemia in Adolescents. J. Clin. Oncol. 34 (28): 3451-3459 (2016). PMID 27507882. [link to RRC of NBRP]
user_report Yamaguchi, S., Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors. Cancer Sci. 105 (4): 402-408 (2014). PMID 24521492. [link to RRC of NBRP]