Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-HIVgp IN-D64V

Expression vector of HIV-1 gag and pol for lentivirus vector packaging. Integrase mutant.

Catalog number RDB08256
Resource name pCAG-HIVgp IN-D64V
Clone info. Expression vector of HIV-1 gag and pol for lentivirus vector packaging. Integrase mutant.
Vector backbone Plasmid
Selectable markers Kan^r
Gene/insert name HIV-1 gag-pol
Depositor|Developer Miyoshi, Hiroyuki |
 
Other clones in our bank HIV-1 (KEGG orthology K22951) |
Lentiviral plasmid list. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction.[link] 
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Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08256 pCAG-HIVgp IN-D64V DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB08256z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB08256.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB09078_A3Ctp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3
Region: CAG pro,insert 5'
Sequence file: RDB09078_A3Cta.seq check
>08256_09078_B1DlF_3_pCAG-F_V3_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNGGNNNGGN NANGGCGGGG TTCGGCTTCT GGCGTGTGAC CGGCGGNNNN
   61 NNNNNNTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TANNNNNNNC TGGGCAACGT
  121 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTG ATTTGATACG TACGGTTTCG
  181 AGCTCAAGCT TCGAATTCGA GATCTGCCGC CGCCATGGGT GCGAGAGCGT CAGTATTAAG
  241 CGGGGGAGAA TTAGATCGAT GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA
  301 TAAATTAAAA CATATAGTAT GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG
  361 CCTGTTAGAA ACATCAGAAG GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA
  421 GACAGGATCN NANNANCTTA GATCNTNNNN TAATACAGTA GCAACCCTCT ATTGTGTGCA
  481 TCANNNGNNA NAGATAAAAG ACACCAAGGA AGCTTTANAC AAGATAGAGG AAGAGCAAAA
  541 NCAAAAGTAN NNNNAAAAGC ACAGCANGCN NNCANCTGAC ACNNGACNCA NCANTCANGT
601 CNNNNNNNNN N
//
Primer: RRE-M_R
Region: RRE,insert 3'
Sequence file: RDB09078_A3Ctb.seq check
>08256_09078_B1DlF_3_RRE-M_R_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG CTGTTGCGCC TNNNTAGCCC TCAGCAAATT GTTCTGCTGC
   61 TGCACTATAC CAGACAATAA TTGTCTGGCC TGTACCGTCA GCGTCATTGA CGCTGCGCCC
  121 ATAGTGCTTC CTGCTGCTCC CAAGAACCCA AGGAACAAAG CTCCTGCGGC CGCTCCGGAA
  181 TTCCATGTGT TAATCCTCAT CCTGTCTACT TGCCACACAA TCATCACCTG CCATCTGTTT
  241 TCCATAATCC CTGATGATCT TTGCTTTTCT TCTTGGCACT ACTTTTANGN NNNTATTATC
  301 TTGTATTACT ACTGNCCNTT NACCTTTCCN NANGAGCTTT NNTGNNNCTT TCNNAACTGG
361 NTNNCNGCTG TCNNNGNNAN ANACCCNNNN ATNNNNNNNN TNN
//
Primer: HIVgp_F1
Region: insert mid
Sequence file: RDB09078_A3Ctc.seq check
>RDB9078_3_HIVgp_R1_F10_16.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGANTCNNTG TGTTAATCCT CNTCCTGTCT ACTTGCCACA
   61 CAATCATCAC CTGCNNTNNG TTTTCCATAA TCCCTGATGA TCTTTGCTTT TCTTCTTGGC
  121 ACTACTTTTA TGTCACTATT ATCTTGTATT ACTACTGCCC CTTCACCTTT CCAGAGGAGC
  181 TTTGCTGGTC CTTTCCAAAC TGGATCTCTG CTGTCCCTGT AATAAACCCG AAAATTTTGA
  241 ATTTTTGTAA TTTGTTTTTG TAATTCTTTA GTTTGTATGT CTGTTGCTAT TATGTCTACT
  301 ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAATT GTGGATGAAT
  361 ACTGCCATTT GTACTGCTGT CTTAAGATGT TCAGCCTGAT CTCTTACCTG TCCTATAATT
  421 TTCTTTAATT CTTTATTCAT AGATTCTATT ACTCCTTGAC TTTGGGGATT GTAGGGAATG
  481 CCAAATTCCT GCTTGATCCC CGCCCACCAA CAGGCGGCCT TAACTGTAGT ACTGGTGAAA
  541 TTGCTGCCAT TGTCTGTATG TACTGTTTTT ACTGGCCATC TTCCTGCTAA TTTTAAGAGG
  601 AAGTATGCTG TTTCTTGCCC TGTCTCTGCT GGAATTACTT CTGCTTCTAT ATATCCACTG
  661 GCTACATGAA CTGCTACCAA GATAACTTTT CCTTCTAAAT GTGTACAAAC TAGCTGCCAT
  721 ATTCCTGGGC TACAGTCTAC TTGTCCATGC ATGGCTTCCC CTTTTANCTG ACATTTATCA
  781 CAGCTGGCTA CTATTTCTTT TGCTACTACN GGTGGTANGT TAAAATCACT AGCCATTGCT
  841 CTCCAATTAC TGTGATATTT CTCATGTTCT TCTTGGGCCT TATCTATTNN NTCNNAAAAN
  901 TAGTACTTTC CTGANTNNNG CACTGANNNA TTTATCTACN NGTTNNTTTN NNNCAATTCC
  961 NTTGTGTGCT GGNANCNNTG NCNNGNANAN NTTTTNNNNT TTTANNNNNN GNNNNNNNAN
1021 TNNANTNANN NANNNNNNAN NNNNNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB09078_A3Ctd.seq check
>08256_09078_3_B1DlF_SV40pro_ori_F_F05_17_ABI24.ab1
    1 NNNNNNNNNN NGNCGCCTCN GCCTCTGNNC TATTCCNGAA GTAGTGAGGA GGCTTTTTTG
   61 GAGGCCTANG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT
  121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
  181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG
  241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC
  301 GAGGCAGCGC GGCTATCGTG GCTGGCCNCN ACNGGCGTTC CTTGCGCAGC TGTGCTNNNN
  361 NNTGTCACTG NANNCNGGAA GGGACTGNNN NNNNTNGNNC NAANNGNCNG NNNNNGNTNN
421 NNNGNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

reference Fukuda, T., Efficient establishment of pig embryonic fibroblast cell lines with conditional expression of the simian vacuolating virus 40 large T fragment. Biosci. Biotechnol. Biochem. 76 (7): 1372-1377 (2012). PMID 22785463.
user report Yoshida, T., Human SMOOTHENED inhibits human immunodeficiency virus type 1 infection. Biochem. Biophys. Res. Commun. 493 (1): 132-138 (2017). PMID 28917838.

2021.05.19

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