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pCAG-HIVgp IN-D64V (#RDB08256)

Expression vector of HIV-1 gag and pol for lentivirus vector packaging. Integrase mutant.

Clone info. Expression vector of HIV-1 gag and pol for lentivirus vector packaging. Integrase mutant.
Vector backbone Plasmid
Selectable markers Kan^r
Growth conditions 37C, LB+Kan
Gene/insert name HIV-1 gag-pol
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence (full) RDB08256zzk02.seq provided by the depositor
Remarks, protocol and/or map (pdf) RDB08256.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08256 pCAG-HIVgp IN-D64V DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG-HIVgp IN-D64V was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08256).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB09078_A3Ctp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HIV-1gag_R (Pr1004)
Region: insert 5'
Sequence file: RDB09078_A3Cte.seq check
>08256_09078_B1DlF_3_HIVgag_R_G02_20_ABI24.ab1
    1 NNNNNNNNNT TNCNTGNNGN CTTTTATCTC TATCCTTTGA TGCACACAAT AGAGGGTTGC
   61 TACTGTATTA TATAATGATC TAAGTTCTTC TGATCCTGTC TGAAGGGATG GTTGTAGCTG
  121 TCCCAGTATT TGTCTACAGC CTTCTGATGT TTCTAACAGG CCAGGATTAA CTGCGAATCG
  181 TTCTAGCTCC CTGCTTGCCC ATACTATATG TTTTAATTTA TATTTTTTCT TTCCCCCTGG
  241 CCTTAACCGA ATTTTTTCCC ATCGATCTAA TTCTCCCCCG CTTAATACTG ACGCTCTCGC
  301 ACCCATGGCG GCGGCAGATC TCGAATTCGA AGCTTGAGCT CGAAACCGTA CGTATCAAAT
  361 CAATTCTTTG CCAAAATGAT GAGACAGCAC AATAACCAGC ACGTTGCCCA GGAGCTGTAN
  421 GAAAAAGAAN AAGGCNNNNN NATGGTTAGC AGANGNNNNA NAGNCNNNNG NCACACNCCN
481 NAAGCCGAAC CCCGNCCTGC CCCGTCCNCC CNNAAGGCAG CNNNNNNNNN N
//
Primer: RRE-M_R (Pr0651)
Region: RRE,insert 3'
Sequence file: RDB09078_A3Ctf.seq check
>08256_09078_B1DlF_3_RRE-M_R_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG CTGTTGCGCC TNNNTAGCCC TCAGCAAATT GTTCTGCTGC
   61 TGCACTATAC CAGACAATAA TTGTCTGGCC TGTACCGTCA GCGTCATTGA CGCTGCGCCC
  121 ATAGTGCTTC CTGCTGCTCC CAAGAACCCA AGGAACAAAG CTCCTGCGGC CGCTCCGGAA
  181 TTCCATGTGT TAATCCTCAT CCTGTCTACT TGCCACACAA TCATCACCTG CCATCTGTTT
  241 TCCATAATCC CTGATGATCT TTGCTTTTCT TCTTGGCACT ACTTTTANGN NNNTATTATC
  301 TTGTATTACT ACTGNCCNTT NACCTTTCCN NANGAGCTTT NNTGNNNCTT TCNNAACTGG
361 NTNNCNGCTG TCNNNGNNAN ANACCCNNNN ATNNNNNNNN TNN
//
Primer: HIVgp_F1 (Pr0131)
Region: insert mid
Sequence file: RDB09078_A3Ctg.seq check
>RDB9078_3_HIVgp_F1_E10_13.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTGCNT TGGGANNCAT TCNAGCACAA CCAGATAAGA
   61 GTGAATCAGA GTTAGTCNGT CAAATAATAG AGCAGTTAAT AAAAAAGGAA AAAGTCTACC
  121 TGGCATGGGT ACCAGCACAC AAAGGAATTG GAGGAAATGA ACAAGTAGAT AAATTGGTCA
  181 GTGCTGGAAT CAGGAAAGTA CTATTTTTAG ATGGAATAGA TAAGGCCCAA GAAGAACATG
  241 AGAAATATCA CAGTAATTGG AGAGCAATGG CTAGTGATTT TAACCTACCA CCTGTAGTAG
  301 CAAAAGAAAT AGTAGCCAGC TGTGATAAAT GTCAGCTAAA AGGGGAAGCC ATGCATGGAC
  361 AAGTAGACTG TAGCCCAGGA ATATGGCAGC TAGTTTGTAC ACATTTAGAA GGAAAAGTTA
  421 TCTTGGTAGC AGTTCATGTA GCCAGTGGAT ATATAGAAGC AGAAGTAATT CCAGCAGAGA
  481 CAGGGCAAGA AACAGCATAC TTCCTCTTAA AATTAGCAGG AAGATGGCCA GTAAAAACAG
  541 TACATACAGA CAATGGCAGC AATTTCACCA GTACTACAGT TAAGGCCGCC TGTTGGTGGG
  601 CGGGGATCAA GCAGGAATTT GGCATTCCCT ACAATCCCCA AAGTCAAGGA GTAATAGAAT
  661 CTATGAATAA AGAATTAAAG AAAATTATAG GACAGGTAAG AGATCAGGCT GAACATCTTA
  721 AGACAGCAGT ACAAATGGCA GTATTCATCC NCAATTTTAA AAGAAAAGGG GGGANTGGGG
  781 GGTACAGTGC ANGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT
  841 TACAAAAACA AATTACNAAA ATTCAAAATT TTCNGGTTTA NTACAGGGNN NNCANANNTC
  901 CNGTTTGGAA NNGNNCNGCN AAGCTNCNCN GNAAAGGNGA ANGGGCAGTA NTAATACNNN
  961 NNNATANTGA NNTAAAANTA NTGNNNNNAA NAAAANNNAA NANNNTCNNN NNNANGGNAA
1021 ANNNNNNNNN N
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB09078_A3Cth.seq check
>08256_09078_3_B1DlF_SV40pro_ori_F_F05_17_ABI24.ab1
    1 NNNNNNNNNN NGNCGCCTCN GCCTCTGNNC TATTCCNGAA GTAGTGAGGA GGCTTTTTTG
   61 GAGGCCTANG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT
  121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
  181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG
  241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC
  301 GAGGCAGCGC GGCTATCGTG GCTGGCCNCN ACNGGCGTTC CTTGCGCAGC TGTGCTNNNN
  361 NNTGTCACTG NANNCNGGAA GGGACTGNNN NNNNTNGNNC NAANNGNCNG NNNNNGNTNN
421 NNNGNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Fukuda, T., Efficient establishment of pig embryonic fibroblast cell lines with conditional expression of the simian vacuolating virus 40 large T fragment. Biosci. Biotechnol. Biochem. 76 (7): 1372-1377 (2012). PMID 22785463. [link to RRC of NBRP]
user_report Yoshida, T., Human SMOOTHENED inhibits human immunodeficiency virus type 1 infection. Biochem. Biophys. Res. Commun. 493 (1): 132-138 (2017). PMID 28917838. [link to RRC of NBRP]