Resource data sheet
Prev. |  Lentiviral vector plasmid > 
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-HIVgp IN-D64V

Expression vector of HIV-1 gag and pol for lentivirus vector packaging. Integrase mutant.

Catalog number RDB08256
Resource name pCAG-HIVgp IN-D64V
Clone info. Expression vector of HIV-1 gag and pol for lentivirus vector packaging. Integrase mutant.
Vector backbone Plasmid
Selectable markers Kan^r
Gene/insert name HIV-1 gag-pol
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database
HIV-1 gag-pol

          Reference sequence
            
           
          Sequence (full) RDB08256zzk02.seq provided by the depositor
          Remarks, protocol and/or map (pdf) RDB08256.pdf
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
          Ordering Please visit Ordering instruction.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08256 pCAG-HIVgp IN-D64V DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCAG-HIVgp IN-D64V was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08256).

          Reference section:

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Lentivirus Vector (English text)
          Featured content Lentivirus Vector (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB09078_A3Ctp1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HIV-1gag_R (Pr1004)
          Region: insert 5'
          Sequence file: RDB09078_A3Cte.seq check
          >08256_09078_B1DlF_3_HIVgag_R_G02_20_ABI24.ab1
              1 NNNNNNNNNT TNCNTGNNGN CTTTTATCTC TATCCTTTGA TGCACACAAT AGAGGGTTGC
             61 TACTGTATTA TATAATGATC TAAGTTCTTC TGATCCTGTC TGAAGGGATG GTTGTAGCTG
            121 TCCCAGTATT TGTCTACAGC CTTCTGATGT TTCTAACAGG CCAGGATTAA CTGCGAATCG
            181 TTCTAGCTCC CTGCTTGCCC ATACTATATG TTTTAATTTA TATTTTTTCT TTCCCCCTGG
            241 CCTTAACCGA ATTTTTTCCC ATCGATCTAA TTCTCCCCCG CTTAATACTG ACGCTCTCGC
            301 ACCCATGGCG GCGGCAGATC TCGAATTCGA AGCTTGAGCT CGAAACCGTA CGTATCAAAT
            361 CAATTCTTTG CCAAAATGAT GAGACAGCAC AATAACCAGC ACGTTGCCCA GGAGCTGTAN
            421 GAAAAAGAAN AAGGCNNNNN NATGGTTAGC AGANGNNNNA NAGNCNNNNG NCACACNCCN
          481 NAAGCCGAAC CCCGNCCTGC CCCGTCCNCC CNNAAGGCAG CNNNNNNNNN N
          //
          Primer: RRE-M_R (Pr0651)
          Region: RRE,insert 3'
          Sequence file: RDB09078_A3Ctf.seq check
          >08256_09078_B1DlF_3_RRE-M_R_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNG CTGTTGCGCC TNNNTAGCCC TCAGCAAATT GTTCTGCTGC
             61 TGCACTATAC CAGACAATAA TTGTCTGGCC TGTACCGTCA GCGTCATTGA CGCTGCGCCC
            121 ATAGTGCTTC CTGCTGCTCC CAAGAACCCA AGGAACAAAG CTCCTGCGGC CGCTCCGGAA
            181 TTCCATGTGT TAATCCTCAT CCTGTCTACT TGCCACACAA TCATCACCTG CCATCTGTTT
            241 TCCATAATCC CTGATGATCT TTGCTTTTCT TCTTGGCACT ACTTTTANGN NNNTATTATC
            301 TTGTATTACT ACTGNCCNTT NACCTTTCCN NANGAGCTTT NNTGNNNCTT TCNNAACTGG
          361 NTNNCNGCTG TCNNNGNNAN ANACCCNNNN ATNNNNNNNN TNN
          //
          Primer: HIVgp_F1 (Pr0131)
          Region: insert mid
          Sequence file: RDB09078_A3Ctg.seq check
          >RDB9078_3_HIVgp_F1_E10_13.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNTGCNT TGGGANNCAT TCNAGCACAA CCAGATAAGA
             61 GTGAATCAGA GTTAGTCNGT CAAATAATAG AGCAGTTAAT AAAAAAGGAA AAAGTCTACC
            121 TGGCATGGGT ACCAGCACAC AAAGGAATTG GAGGAAATGA ACAAGTAGAT AAATTGGTCA
            181 GTGCTGGAAT CAGGAAAGTA CTATTTTTAG ATGGAATAGA TAAGGCCCAA GAAGAACATG
            241 AGAAATATCA CAGTAATTGG AGAGCAATGG CTAGTGATTT TAACCTACCA CCTGTAGTAG
            301 CAAAAGAAAT AGTAGCCAGC TGTGATAAAT GTCAGCTAAA AGGGGAAGCC ATGCATGGAC
            361 AAGTAGACTG TAGCCCAGGA ATATGGCAGC TAGTTTGTAC ACATTTAGAA GGAAAAGTTA
            421 TCTTGGTAGC AGTTCATGTA GCCAGTGGAT ATATAGAAGC AGAAGTAATT CCAGCAGAGA
            481 CAGGGCAAGA AACAGCATAC TTCCTCTTAA AATTAGCAGG AAGATGGCCA GTAAAAACAG
            541 TACATACAGA CAATGGCAGC AATTTCACCA GTACTACAGT TAAGGCCGCC TGTTGGTGGG
            601 CGGGGATCAA GCAGGAATTT GGCATTCCCT ACAATCCCCA AAGTCAAGGA GTAATAGAAT
            661 CTATGAATAA AGAATTAAAG AAAATTATAG GACAGGTAAG AGATCAGGCT GAACATCTTA
            721 AGACAGCAGT ACAAATGGCA GTATTCATCC NCAATTTTAA AAGAAAAGGG GGGANTGGGG
            781 GGTACAGTGC ANGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT
            841 TACAAAAACA AATTACNAAA ATTCAAAATT TTCNGGTTTA NTACAGGGNN NNCANANNTC
            901 CNGTTTGGAA NNGNNCNGCN AAGCTNCNCN GNAAAGGNGA ANGGGCAGTA NTAATACNNN
            961 NNNATANTGA NNTAAAANTA NTGNNNNNAA NAAAANNNAA NANNNTCNNN NNNANGGNAA
          1021 ANNNNNNNNN N
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR_KanR
          Sequence file: RDB09078_A3Cth.seq check
          >08256_09078_3_B1DlF_SV40pro_ori_F_F05_17_ABI24.ab1
              1 NNNNNNNNNN NGNCGCCTCN GCCTCTGNNC TATTCCNGAA GTAGTGAGGA GGCTTTTTTG
             61 GAGGCCTANG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT
            121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
            181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG
            241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC
            301 GAGGCAGCGC GGCTATCGTG GCTGGCCNCN ACNGGCGTTC CTTGCGCAGC TGTGCTNNNN
            361 NNTGTCACTG NANNCNGGAA GGGACTGNNN NNNNTNGNNC NAANNGNCNG NNNNNGNTNN
          421 NNNGNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          reference Fukuda, T., Efficient establishment of pig embryonic fibroblast cell lines with conditional expression of the simian vacuolating virus 40 large T fragment. Biosci. Biotechnol. Biochem. 76 (7): 1372-1377 (2012). PMID 22785463.
          user_report Yoshida, T., Human SMOOTHENED inhibits human immunodeficiency virus type 1 infection. Biochem. Biophys. Res. Commun. 493 (1): 132-138 (2017). PMID 28917838.

          2023.05.22

          GNP_filter3_RDBDEP_html_230512.pl