Resource data sheet
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pCAG-HIVgp IN-D64V

Expression vector of VSV G protein HIV-1 gag and pol. Integrase mutant.

Catalog number RDB08256
Resource name pCAG-HIVgp IN-D64V
Clone info. Expression vector of VSV G protein HIV-1 gag and po for packagingl. Integrase mutant.
Vector backbone Plasmid
Selectable markers Kan^r
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB09078_A3Cr.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: RDB09078_A3Cra.seq
>08256_09078_A3Cr_pAxCA_F1_E12_15.ab1
    1 ACGGACAATA GCAACCGTCT GCTCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
   61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTGATT TGATACGTAC
  121 GGTTTCGAGC TCAAGCTTCG AATTCGAGAT CTGCCGCCGC CATGGGTGCG AGAGCGTCAG
  181 TATTAAGCGG GGGAGAATTA GATCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA
  241 AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA
  301 ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT
  361 CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT
  421 GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG
  481 AGCAAAACAA AAGTAAGAAA AAAGCACAGC AAGCAGCAGC TGACACAGGA CACAGCAATC
  541 AGGTCAGCCA AAATTACCCT ATAGTGCAGA ACATCCAGGG GCAAATGGTA CATCAGGCCA
  601 TATCACCTAG AACTTTAAAT GCATGGGTAA AAGTAGTAGA AGAGAAGGCT TTCAGCCCAG
  661 AAGTGATACC CATGTTTTCA GCATTATCAG AAGGAGCCAC CCCACAAGAT TTAAACACCA
  721 TGCTAAACAC AGTGGGGGGA CATCAAGCAG CCATGCAAAT GTTAAAAGAG ACCATCAATG
  781 AGGAAGCTGC AGAATGGGAT AGAGTGCATC CAGTGCATGC AGGGCCTATT GCACCAGGCC
  841 AGATGAGAGA ACCAAGGGGA AGTGACATAG CAGGAACTAC TAGTACCCTT CAGGAACAAA
  901 TAGGATGGAT GACACATAAT CCACCTATCC CAGTAGGAGA AATCTATAAA AGATGGATAA
  961 TCCTGGGATT AAATAAAATA GTAAGAATGT ATAGCCCTAC CAGCATTCTG GACATAAGAC
 1021 AAGGACCAAA GGAACCCCTT TAGAGACTAT GTAGACCGAT TCTATAAAAC TCTAAGAGCC
 1081 GAGCAAGCTT TCACAGAGGT AAAAAAATTG GATGACAGAA ACTGATGTCG AAAATGCCGG
1141 ACCCAGATTG TAGACTATTT TTTTAAAAGG CCATTGGGGG AACCCAAG
//
Primer: RDB09078_A3Crb.seq
>08256_09078_A3Cr_HIVgp_F1_D09_12.ab1
    1 GCCATGGTTT AAAGACTCAC AATATGCATT GGGAATCATT CAAGCACAAC CAGATAAGAG
   61 TGAATCAGAG TTAGTCAGTC AAATAATAGA GCAGTTAATA AAAAAGGAAA AAGTCTACCT
  121 GGCATGGGTA CCAGCACACA AAGGAATTGG AGGAAATGAA CAAGTAGATA AATTGGTCAG
  181 TGCTGGAATC AGGAAAGTAC TATTTTTAGA TGGAATAGAT AAGGCCCAAG AAGAACATGA
  241 GAAATATCAC AGTAATTGGA GAGCAATGGC TAGTGATTTT AACCTACCAC CTGTAGTAGC
  301 AAAAGAAATA GTAGCCAGCT GTGATAAATG TCAGCTAAAA GGGGAAGCCA TGCATGGACA
  361 AGTAGACTGT AGCCCAGGAA TATGGCAGCT AGTTTGTACA CATTTAGAAG GAAAAGTTAT
  421 CTTGGTAGCA GTTCATGTAG CCAGTGGATA TATAGAAGCA GAAGTAATTC CAGCAGAGAC
  481 AGGGCAAGAA ACAGCATACT TCCTCTTAAA ATTAGCAGGA AGATGGCCAG TAAAAACAGT
  541 ACATACAGAC AATGGCAGCA ATTTCACCAG TACTACAGTT AAGGCCGCCT GTTGGTGGGC
  601 GGGGATCAAG CAGGAATTTG GCATTCCCTA CAATCCCCAA AGTCAAGGAG TAATAGAATC
  661 TATGAATAAA GAATTAAAGA AAATTATAGG ACAGGTAAGA GATCAGGCTG AACATCTTAA
  721 GACAGCAGTA CAAATGGCAG TATTCATCCA CAATTTTAAA AGAAAAGGGG GGATTGGGGG
  781 GTACAGTGCA GGGGAAAAGA AATAGTAGAC ATAATAGCAA CAGACATACA AACTAAAAGA
  841 ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA
  901 TCCAGTTTGG AAAGGACCAG CAAAGCTCCT CTGGAAAGGT GAAGGGGCAG TAGTAATACA
  961 AGATAATAGT GACATAAAAG TAGTGCCAAG AAGAAAAGCA AAGATCATCA GGGATTATGG
 1021 AAAAACAGAT GGGCAAGTGA TGATTGTGTG GCAAGGTAGA CAGGGATGGA GGATTAACAC
 1081 AATGGAATTC CCGAGCGGGC CGGCAGGAGC TTTGTTCTTT GGGTTCTTGG GAGCAGCAGG
 1141 AGCACTATGG GCCGCAGCGG TTCAATTGAC CGCTTGGAAC GGTTACCAGG CCCAGGAACC
1201 A
//
Primer: RDB09078_A3Crc.seq
>08256_09078_A3Cr_HIVgp_R1_E09_15.ab1
    1 GAGCAATTCT TGCGGCCGCC TCCGGAATTC CATGTGTTAA TCCTCATCCT GTCTACTTGC
   61 CACACAATCA TCACCTGCCA TCTGTTTTCC ATAATCCCTG ATGATCTTTG CTTTTCTTCT
  121 TGGCACTACT TTTATGTCAC TATTATCTTG TATTACTACT GCCCCTTCAC CTTTCCAGAG
  181 GAGCTTTGCT GGTCCTTTCC AAACTGGATC TCTGCTGTCC CTGTAATAAA CCCGAAAATT
  241 TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT ATGTCTGTTG CTATTATGTC
  301 TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCCCCCT TTTCTTTTAA AATTGTGGAT
  361 GAATACTGCC ATTTGTACTG CTGTCTTAAG ATGTTCAGCC TGATCTCTTA CCTGTCCTAT
  421 AATTTTCTTT AATTCTTTAT TCATAGATTC TATTACTCCT TGACTTTGGG GATTGTAGGG
  481 AATGCCAAAT TCCTGCTTGA TCCCCGCCCA CCAACAGGCG GCCTTAACTG TAGTACTGGT
  541 GAAATTGCTG CCATTGTCTG TATGTACTGT TTTTACTGGC CATCTTCCTG CTAATTTTAA
  601 GAGGAAGTAT GCTGTTTCTT GCCCTGTCTC TGCTGGAATT ACTTCTGCTT CTATATATCC
  661 ACTGGCTACA TGAACTGCTA CCAAGATAAC TTTTCCTTCT AAATGTGTAC AAACTAGCTG
  721 CCATATTCCT GGGCTACAGT CTACTTGTCC ATGCATGGCT TCCCCTTTTA GCTGACATTT
  781 ATCACAGCTG GCTACTATTT CTTTTGCTAC TACAGGTGGT AGGTTAAAAT CACTAGCCAT
  841 TGCTCTCCAA TTACTGTGAT ATTTCTCATG TTTCTTCTTG GGCCTTATCT ATTCCATCTA
  901 AAAAATAGTA CTTTTCCTGA TTCCAGCACT GACCAATTTA TCTACTTGTT CATTTCCTCC
  961 AATTTCCTTT GTGTGCTGGT ACCCATGCCC AGGTAGACTT TTTCCTTTTT TTAATTACTG
 1021 CTCTATTAAT TGACTGACTA ACCTCTGATT CACTCTTTAT CTGGTTGTGC TTGAATGAAT
 1081 TTCCCAAATG CATTATTGTG AGTCTGGTCA CTATGATTTA CCTTCTAATT CCCGGATTCC
 1141 TGCCAAAGCT AGATGAAATT GGCTTTGTTA ACTTTCAAGG TCCCCTTTCC TCGTGAATTG
1201 ATGGTGTG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

user report Yoshida, T., Human SMOOTHENED inhibits human immunodeficiency virus type 1 infection. Biochem Biophys Res Commun. 2017 Sep 14. pii: S0006-291X(17)31823-5 (2017). [Epub ahead of print] PMID 28917838.

2017.10.23

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