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CS-UbC-RfA-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by UbC promoter by Gateway(R) cloning with Venus fluorescent protein marker.

Catalog number RDB08255
Resource name CS-UbC-RfA-IRES2-Venus
Clone info. Backbone vector plasmid of lentivirus construct driven by UbC promoter by Gateway(R) cloning with Venus fluorescent protein marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-UbC-RfA-IRES2-Venus ()
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering Please visit Ordering instruction.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08255 CS-UbC-RfA-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB08255z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB08255.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12132_A9Cip1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: UbCpro_F
Sequence file: RDB12132_A9Cia.seq check
>08255_12132_A9Ci_UbCpro_F_A04_01_ABI08.ab1
    1 AGACATTTGA AAATTTTCAG TGTTAGACTA GTAAATTGTC CGCTAAATTC TGGCCGTTTT
   61 TGGCTTTTTT GTTAGACCGG ACCGTGTTTA GCGCTACCGG TCTCGAGAAT TCTGCAGCGG
  121 CCATCACAAG TTTGTACAAA AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA
  181 TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC
  241 AGTCACTATG GCGGCCGCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA
  301 TAATGTGTGG ATTTTGAGTT AGGATCCGTC GAGATTTTCA GGAGCTAAGG AAGCTAAAAT
  361 GGAGAAAAAA ATCACTGGAT ATACCACCGT TGATATATCC CAATGGCATC GTAAAGAACA
  421 TTTTGAGGCA TTTCAGTCAG TTGCTCAATG TACCTATAAC CAGACCGTTC AGCTGGATAT
  481 TACGGCCTTT TTAAAGACCG TAAAGAAAAA TAAGCACAAG TTTTATCCGG CCTTTATTCA
  541 CATTCTTGCC CGCCTGATGA ATGCTCATCC GGAATTCCGT ATGGCAATGA AAGACGGTGA
  601 GCTGGTGATA TGGGATAGTG TTCACCCTTG TTACACCGTT TTCCATGAGC AAACTGAAAC
  661 GTTTTCATCG CTCTGGAGTG AATACCACGA CGATTTCCGG CAGTTTCTAC ACATATATTC
  721 GCAAGATGTG GCGTGTTACG GTGAAAACCT GGCCTATTTC CCTAAAGGGT TTATTGAGAA
  781 TATGTTTTTC GTCTCAGCCA ATCCCTGGGT GAGTTTCACC AGTTTTGATT TAAACGTGGC
  841 CAATATGGAC AACTTCTTCG CCCCCGTTTT CACCATGGGC AAATATTATA CGCAAGGCGA
  901 CAAGGTGCTG ATGCCGCTGG CGATTCAGGT TCATCATGCC GTTTGTGATG GCTTCCATGT
  961 CGGCAGGAAT GCTTTAATGA AATTACAACA GTACTGCGAT GAGTGGCCAG GGCGGGGGCG
 1021 TAAAACGCGT GGGATCCGGC TTACTAAAAA GCCCAGAATA ACAGTATGCG GTAATTTGCG
 1081 CGCTGATTTT TTGGCGGTAT AGGAAATATA ATACTTGAAT ATGTATACCG AAAGTATGTC
1141 AAAAGAGGTA TGCTTATTGA AGCCAAGCCG TTATTTACCA GTGGAACACG G
//
Primer: Cm_C_F
Sequence file: RDB12132_A9Cib.seq check
>08255_12132_A9Ci_Cm_C_F_B04_02_ABI08.ab1
    1 CAAGAACGCG GTGGGACCGG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT
   61 GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT
  121 ATGCTATGAA GCAGCGTATT ACAGTGACAG TTGACAGCGA CAGCTATCAG TTGCTCAAGG
  181 CATATATGAT GTCAATATCT CCGGTCTGGT AAGCACAACC ATGCAGAATG AAGCCCGTCG
  241 TCTGCGTGCC GAACGCTGGA AAGCGGAAAA TCAGGAAGGG ATGGCTGAGG TCGCCCGGTT
  301 TATTGAAATG AACGGCTCTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG
  361 TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA
  421 TTGACACGCC CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA
  481 AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA
  541 CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC
  601 ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG
661 GCTCCCTTAT ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGA
//
Primer: IRES-F
Sequence file: RDB12132_A9Cic.seq check
>08255_12132_A9Ci_IRES-F_C04_03_ABI08.ab1
    1 GGCAGTCCCT TTCATGGTGT TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC CCCCGAACCA
   61 CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC
  661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT
  721 ACCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
  781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA GAATTCAGGG
  841 CCTCTCGAGC CTCTAGAGTT AACATCGAGG GATCAAGCTT ATCGATAATC AACCCTCTGG
  901 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT
  961 GTGGATACGC TGCTTTAATG CCTTTGGTAT CATGCTATTG CTTCCCGGTA TGGCTTTCAA
 1021 TTTTTCTTCT CCTGTATAAA TCCCTGGGTT TGCCTGGTCT CTTATGAGGA GTTGTGGGCC
 1081 CCGTTGTCAA GGCCAACCGT TGCGTGATGT GCAACTGTTG TTTGGCTGGA ACGCACCCCC
1141 ACTTGGTTGG GGGCATTTGA CCTACCAGCT TGTTTCTAAG CTTCCTTATC
//
Primer: CMV-Forward
Sequence file: RDB12132_A9Cid.seq check
>08255_12132_A9Ci_CMV-Forward_D04_04_ABI08.ab1
    1 AGGGATCCAC CTTCCCGCCC CATTGACGGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC
  121 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA
  181 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA
  241 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA
  301 AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA
  361 GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG
  421 AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA
  481 AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA
  541 GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA
  601 GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT
  661 TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA
  721 AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG
  781 CGGCCGCTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAAATTGGA GAAGTGAATT
  841 ATATAAATAT AAAGTAGTAA AAATTGAACC ATTAAGGAGT AGCACCCACC AAGGCAAAGA
  901 GAAGAGTGGT GCAGAGAGAA AAAAGAGCAG TGGGGAATAG GAGCTTTTGT TTCCTTGGGT
  961 TTCTTTGGGA GCAGCAGGGA AGCACTATGG GGCGGCAGCG TTCAATGGAC GCTGACGGTA
 1021 CAAGGCCCAG AACAATTTAT TGTTCTGGTA TTAGTTGCAG CCAGCAGAAA CAAATTTTGC
 1081 CTGGAAGGGG CTATATTTGG AAGGGCCGCA ACAGCATCCT GTTTGCAAAC TCTCAACAGT
 1141 CTGGGGGCAT TCAAGCAGCT CCCAGGCCAA AGAAATTCCT TGGCCTTGTT GTAGGAAGAG
1201 ATATGATGCT
//
Primer: BGH_rev2
Sequence file: RDB12132_A9Cie.seq check
>08255_12132_A9Ci_BGH_rev2_E04_05_ABI08.ab1
    1 TTCGTAGGCG GGGTTCAGGA GCACGGGGGA GGGGCAAACA ACAGATGGCT GGCAACTAGA
   61 AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG
  121 ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA
  181 CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG
  241 AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT
  301 CTCGGGCCAT GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT
  361 CCGGGTCATC CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT
  421 TGGCTTCTTC TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA
  481 GTGGATATCT GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG
  541 TGTGGTAGAT CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA
  601 GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC
  661 TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC
  721 AGGCACAATC AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA
  781 GGTCTCGACG GTATCGATGC GGGGAGGCGG CCCAAAGGGA GATCCGACTC GTCTGAGGGC
  841 GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG
  901 CTGGATTGAG GGCCGAAAGG GACGTAGCAA AAAGGACGTC CCGCGCAGAA TCCAGGTGGC
  961 AACACAGGGC GAGCAGCCAT GGGAAAGGAC GTCAGCTTCC CGACAACACC ACGGAAATTG
 1021 TCAGTGCCCA ACAGCCGAGC CCCTGGTCCA GCAGCGGGGC CAAGGCAGCG GGCGATGAAG
 1081 TTCCGCCGTG GGCAATAGGG AGGGAAAAGG GCGAAGGTTC CGAAGAGCTG GACCAGGTGT
1141 GCCAATGCCC CAACCAGTGG GGATTGCCGT CCAGACA
//
Primer: SV40pro_F
Sequence file: RDB12132_A9Cif.seq check
>08255_12132_A9Ci_SV40pro_F_F04_06_ABI08.ab1
    1 CCTTCGGGGC CCCGGACATT CAGGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
   61 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA
  121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA
  181 TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG
  241 AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG
  301 TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA
  361 ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG
  421 TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC
  481 CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG
  541 AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT
  601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA
  661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA
  721 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT
  781 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT
  841 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
  901 ACAACATACG AGCCGGAAGC ATAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT
  961 CACATTAATT GCGTTGCGCT CACTGCCCCG CTTTCCAGTC GGGAAAACCT GTCGTGCCAG
 1021 CTGGCAATTA ATGAATTCGG GCCCAACGCG CCGGGGAGAG GCGGTTTGCG TAATTGGGGC
 1081 GCTCTTTCGC TTCCTTCGCT CACTGAACTC GCTGCGGCTT CGGTCGCTCG GCTGCGGCGA
 1141 GCGGGTATCA GGCCTCCACT CAAAGGGCCG GTTTAAATAC CGCGGTTTAA TTCTCCAACC
1201 A
//
Primer: env_F
Sequence file: RDB12132_A9Cig.seq check
>08255_12132_A9Ci_env_F_G04_07_ABI08.ab1
    1 GCGCGGGGGC CGGGATCCAT TCGATTAGTG AACGGATCTA CAAATGGCAG TATTCATCCA
   61 CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT
  121 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT
  181 TCGGGTTTAT TACAGGGACA GCAGAAATTC ACTTTGAATT CTAGCTCGAG ATCGGGAGAT
  241 CTGGCCTCCG CGCCGGGTTT TGGCGCCCCC CGCGGGCGCC CCCCTCCTCA CGGCGAGCGC
  301 TGCCACGTCA GACGAAGGGC GCAGCGAGCG TCCTGATCCT TCCGCCCGGA CGCTCAGGAC
  361 AGCGGCCCGC TGCTCATAAG ACTCGGCCTT AGAACCCCAG TATCAGCAGA AGGACATTTT
  421 AGGACGGGAC TTGGGTGACT CTAGGGCACT GGTTTTCTTT CCAGAGAGCG GAACAGGCGA
  481 GGAAAAGTAG TCCCTTCTCG GCGATTCTGC GGAGGGATCT CCGTGGGGCG GTGAACGCCG
  541 ATGATTATAT AAGGACGCGC CGGGTGTGGC ACAGCTAGTT CCGTCGCAGC CGGGATTTGG
  601 GTCGCGGTTC TTGTTTGTGG ATCGCTGTGA TCGTCACTTG GTGAGTAGCG GGCTGCTGGG
  661 CTGGCCGGGG CTTTCGTGGC CGCCGGGCCG CTCGGTGGGA CGGAAGCGTG TGGAGAGACC
  721 GCCAAGGGCT GTAGTCTGGG TCCGCGAGCA AGGTTGCCCT GAACTGGGGG TTGGGGGGAG
  781 CGCAGCAAAA TGGGCGGCTG TTCCCGAGTC TTGAATGGAA GACGCTTGTG AGGCGGGCTG
  841 TGAGGTCGTT GAAACAAGGT GGGGGGCATG GTGGGCGGCA AGAACCCAAG GTCTTGAGCC
  901 TTCGCTAATG CGGGGAAAGC TCTTATTCGG GTGAGATGGG CTGGGGGCAC CATCTGGGGA
  961 CCCTGACGTG AAGCTTTGTC ACCTGACTGG AAAACTCGTT TGTCGTCTGT GCCGGGGGGC
 1021 GGCAGTTAAT GGGCGTGCGT TGGGCAATGC CACCCGGTAA CCTTGGGGAA GCGCGCGGCC
1081 CTTCGGTCGT GGTCGT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Ichise, H., NK Cell Alloreactivity against KIR-Ligand-Mismatched HLA-Haploidentical Tissue Derived from HLA Haplotype-Homozygous iPSCs. Stem Cell Reports 9 (3): 853-867 (2017). PMID 28867344.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl