Resource data sheet
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CS-UbC-RfA-IRES2-Venus

Backbone vector of lentivirus construct driven by UbC promoter by Gateway(R) cloning with Venus fluorescent protein marker.

Catalog number RDB08255
Resource name CS-UbC-RfA-IRES2-Venus
Clone info. Backbone vector of lentivirus construct driven by UbC promoter by Gateway(R) cloning with Venus fluorescent protein marker.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-UbC-RfA-IRES2-Venus ()
Selectable markers Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08255 CS-UbC-RfA-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08255z.seq from Depositor
Electronic file PDF RDB08255.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12132_A3Egp1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: UbCpro_F RDB12132_A3Ega_3.seq
>08255_12132_A3Eg_UbCpro_F_G01_07_ABI08.ab1
    1 TGACTAAGTA TTTTCAGTGT TAGACTAGTA AATTGTCCGC TAAATTCTGG CCGTTTTTGG
   61 CTTTTTTGTT AGACCGGACC GTGTTTAGCG CTACCGGTCT CGAGAATTCT GCAGCGGCCA
  121 TCACAAGTTT GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT
  181 TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT
  241 CACTATGGCG GCCGCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATAA
  301 TGTGTGGATT TTGAGTTAGG ATCCGTCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGGA
  361 GAAAAAAATC ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT
  421 TGAGGCATTT CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC
  481 GGCCTTTTTA AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT
  541 TCTTGCCCGC CTGATGAATG CTCATCCGGA ATTCCGTATG GCAATGAAAG ACGGTGAGCT
  601 GGTGATATGG GATAGTGTTC ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT
  661 TTCATCGCTC TGGAGTGAAT ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA
  721 AGATGTGGCG TGTTACGGTG AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT
  781 GTTTTTCGTC TCAGCCAATC CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCAA
  841 TATGGACAAC TTCTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC AAGGCGACAA
  901 GGTGCTGATG CCGCTGGCGA TTCAGGTTCA TCATGCCGTT TGTGATGGCT TCCATGTCGG
  961 CAGAATGCTT AATGAATTAC AACAGTACTG CGATGAGTGG CAGGCGGGGC GTAAACGCGT
 1021 GGATCCGGCT TACTAAAAAG CCAGATACAG TATGCGTATT TGCGCGCTGA TTTTTGCGTA
 1081 TAAGAATAAT ATAACTGGAT ATGTATACTC GAGTATGGTC AAAAAGAAGG TAATGCCTAA
1141 TGAAAGCCAA GCCG
//
Primer: Cm_C_F RDB12132_A3Egb_3.seq
>08255_12132_A3Eg_Cm_C_F_H01_08_ABI08.ab1
    1 GACATTGCGG TGGATCGGGC TTACTAAAAG CCAGATAACA GTATGCGTAT TTGCGCGCTG
   61 ATTTTTGCGG TATAAGAATA TATACTGATA TGTATACCCG AAGTATGTCA AAAAGAGGTA
  121 TGCTATGAAG CAGCGTATTA CAGTGACAGT TGACAGCGAC AGCTATCAGT TGCTCAAGGC
  181 ATATATGATG TCAATATCTC CGGTCTGGTA AGCACAACCA TGCAGAATGA AGCCCGTCGT
  241 CTGCGTGCCG AACGCTGGAA AGCGGAAAAT CAGGAAGGGA TGGCTGAGGT CGCCCGGTTT
  301 ATTGAAATGA ACGGCTCTTT TGCTGACGAG AACAGGGGCT GGTGAAATGC AGTTTAAGGT
  361 TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA GTGATATTAT
  421 TGACACGCCC GGGCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC TGTCAGATAA
  481 AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC GCATGATGAC
  541 CACCGATATG GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA
  601 CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG
  661 CTCCCTTATA CACAGCCAGT CTGCAGGTCG ACCATAGTGA CTGGATATGT TGTGTTTTAC
  721 AGTATTATGT AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT
  781 TTTACGTTTC TCGTTCAGCT TTCTTGTACA AAGTGGTGAT GGCCGCGGAT CCGCCCCTCT
  841 CCCTCCCCCC CCCCTAACGT TACTGGCCGA AGCCGCTTGG AATAAGGCCG GTGTGCGTTT
  901 GTCTATATGT TATTTTCCAC CATATTGCCG TCTTTTGGCA ATGTGAGGGC CCGGGAACCC
  961 TGGGCCCTGG TCTTCTTGAC GAGCATTCCC TAGGGGTCTT TTCCCCTCTC GCCAAAGGAA
 1021 TGCAAGGTCT GTTGAATGGT CGTGAAAGGG AAGGCAGTTT CTCTGGGAAG CTTTCTTGGA
 1081 AGGACAACAC GTCTGTAGCG ATCTCTTTGC AGCAGCCGAC TCTCCTCACT TGGCCGACAG
1141 GGGTGTCCTC CTGGCCGGTC CT
//
Primer: IRES-F RDB12132_A3Egc_3.seq
>08255_12132_A3Ae_IRES-F_G04_19.ab1
    1 CAGTCCTTTA TGTGTTTAGT CGAGGTTAAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA
   61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGTG AGCAAGGGCG
  121 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
  181 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA
  241 AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG
  301 GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
  361 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA
  421 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC
  481 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT
  541 ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGCCAACT
  601 TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC TACCAGCAGA
  661 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT
  721 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA
  781 CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTAAGAATTC AAGGCCTCTC
  841 GAGCCTCTAG AGTTAACATC GAGGGATCAA GCTTATCGAT AATCAACCTC TGGATTACAA
  901 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA
  961 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC
 1021 CTTTGTATAA ATCCTGGATG CTGTCTCTTT TATGAGGAGT TGTGGCCCGT TGTCAGGCCA
 1081 CGTGACGTGA TGTGCACTGT GATTGGCTGG ACCGCACCCC CCACCTGGTT GGGGGGCCAA
1141 TTTGGCCCAA CCAC
//
Primer: CMV-Forward RDB12132_A3Egd_3.seq
>08255_12132_A3Eg_CMV-Forward_A02_01_ABI08.ab1
    1 CGATAAACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
  121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
  181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
  241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
  301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
  361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
  421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
  481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
  541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
  601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
  661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
  721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGCGGCCGCT
  781 GATCTTCAGA CCTGGAGGAG GAGATATGAG GGACAATTGG AGAAGTGAAT TATATAAATA
  841 TAAAGTAGTA AAAATTGAAC CATTAGGAGT AGCACCCACC AAGGCAAAGA GAAGAGTGGT
  901 GCAGAGAGAA AAAAAGAGCA GTGGGAATAG GAGCTTTGTT CCTTGGGTTC TTGGGAGCAG
  961 CAGGAAGCAC TATGGGCGCA GCGTCAATGA CGCTGACGGT ACAGGGCCCA GACATTTATT
 1021 GTCTGGTATA GTGCAGCAGC AGAACAATTT GCTGGAGGCT ATTGAGCGGC AACAGCAATC
1081 TGATGGCAAA CTTCACAGGT TCTGGGGGCC AATCAAAGCC AAGGCCTCCC AAG
//
Primer: BGH_rev2 RDB12132_A3Ege_3.seq
>08255_12132_A3Eg_BGH_rev2_B02_02_ABI08.ab1
    1 CATGCGAAGG GTTCAGGGTA GGGCACGGGG GGGGGGCAAC AACAGATGGC TGGCAACTAG
   61 AAGGCACAGT CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT
  121 GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT
  181 ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA
  241 GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC
  301 TCTCGGGCCA TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC
  361 TCCGGGTCAT CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA
  421 TTGGCTTCTT CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC
  481 AGTGGATATC TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTAGCCTTGT
  541 GTGTGGTAGA TCCACAGATC AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC
  601 AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT
  661 CTTAAAGGTA CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC
  721 CAGGCACAAT CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT
  781 AGGTCTCGAC GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG
  841 CGAAGGCGAA GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC
  901 GCTGGATTGA GGGCCGAAGG GACGTAGCAG AAAGGACGTC CCGCGCAGAA TCCAGGTGGC
  961 AACACAGGCG AGCAGCCATG GAAGGGACGT CAGCTTCCCC GACACACCAC GGAAATTGTC
 1021 AGTGCCCACA GCCGAGCCCC TGTCCAGCAG CGGGCCAGCA GGCGGCGATG AGTTTCGTCG
 1081 TGCATTAGGG AGGGGGAAGC GAAGTCCTGA AGGAGCTGAC AGGTGGATGG CCAATGACCT
1141 CCAACG
//
Primer: SV40pro_F RDB12132_A3Egf_3.seq
>08255_12132_A3Eg_SV40pro-F_C02_03_ABI08.ab1
    1 GCTCCGGTTC TTTTGGCAAT CAGAAGGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
   61 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA
  121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA
  181 TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG
  241 AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG
  301 TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA
  361 ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG
  421 TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC
  481 CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG
  541 AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT
  601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA
  661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA
  721 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT
  781 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT
  841 TGGCGTAAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA
  901 CACAACATAC GAGCCGGAAG CATAAAAGTG TAAAGCCCTG GGGTGCCTAA TGAGTGAGCT
  961 AACTCACATT AATTGCGTTT GCGCTCACTG CCCCGCCTTT CCAGTCGGGA AAACCTGTCG
 1021 TGCCCAGCTG CATTTAATGA ATCGGGCCCA ACGCGCGGGG GAGAGCGGTT TTGCCGTATG
 1081 GGCGCTCTCC GCTTCCTCGC TTCACTGACT CGCTGCGCTC GGTTCGTTCG GGCCTTGCGG
1141 GCGAAGCGGG GATATCTCAG AGC
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Ichise, H., NK Cell Alloreactivity against KIR-Ligand-Mismatched HLA-Haploidentical Tissue Derived from HLA Haplotype-Homozygous iPSCs. Stem Cell Reports 9 (3): 853-867 (2017). PMID 28867344.

2018.10.22

GNP_filter3_RDBDEP_html_181012.pl