RDB08255 - CS-UbC-RfA-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by UbC promoter by Gateway(R) cloning with Venus fluorescent protein marker.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution	RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内)

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的提供条件	利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。宮脇敦史博士による提供承諾書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08255	CS-UbC-RfA-IRES2-Venus	DNA solution

Sequence	RDB08255z.seq
Remarks, protocol and/or map (pdf)	RDB08255.pdf

Featured content	Lentivirus Vector (English text)
Featured content	Lentivirus Vector (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: UbCpro_F Sequence file: RDB12132_A9Cia.seq
>08255_12132_A9Ci_UbCpro_F_A04_01_ABI08.ab1 1 AGACATTTGA AAATTTTCAG TGTTAGACTA GTAAATTGTC CGCTAAATTC TGGCCGTTTT 61 TGGCTTTTTT GTTAGACCGG ACCGTGTTTA GCGCTACCGG TCTCGAGAAT TCTGCAGCGG 121 CCATCACAAG TTTGTACAAA AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA 181 TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC 241 AGTCACTATG GCGGCCGCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA 301 TAATGTGTGG ATTTTGAGTT AGGATCCGTC GAGATTTTCA GGAGCTAAGG AAGCTAAAAT 361 GGAGAAAAAA ATCACTGGAT ATACCACCGT TGATATATCC CAATGGCATC GTAAAGAACA 421 TTTTGAGGCA TTTCAGTCAG TTGCTCAATG TACCTATAAC CAGACCGTTC AGCTGGATAT 481 TACGGCCTTT TTAAAGACCG TAAAGAAAAA TAAGCACAAG TTTTATCCGG CCTTTATTCA 541 CATTCTTGCC CGCCTGATGA ATGCTCATCC GGAATTCCGT ATGGCAATGA AAGACGGTGA 601 GCTGGTGATA TGGGATAGTG TTCACCCTTG TTACACCGTT TTCCATGAGC AAACTGAAAC 661 GTTTTCATCG CTCTGGAGTG AATACCACGA CGATTTCCGG CAGTTTCTAC ACATATATTC 721 GCAAGATGTG GCGTGTTACG GTGAAAACCT GGCCTATTTC CCTAAAGGGT TTATTGAGAA 781 TATGTTTTTC GTCTCAGCCA ATCCCTGGGT GAGTTTCACC AGTTTTGATT TAAACGTGGC 841 CAATATGGAC AACTTCTTCG CCCCCGTTTT CACCATGGGC AAATATTATA CGCAAGGCGA 901 CAAGGTGCTG ATGCCGCTGG CGATTCAGGT TCATCATGCC GTTTGTGATG GCTTCCATGT 961 CGGCAGGAAT GCTTTAATGA AATTACAACA GTACTGCGAT GAGTGGCCAG GGCGGGGGCG 1021 TAAAACGCGT GGGATCCGGC TTACTAAAAA GCCCAGAATA ACAGTATGCG GTAATTTGCG 1081 CGCTGATTTT TTGGCGGTAT AGGAAATATA ATACTTGAAT ATGTATACCG AAAGTATGTC 1141 AAAAGAGGTA TGCTTATTGA AGCCAAGCCG TTATTTACCA GTGGAACACG G //
Primer: Cm_C_F Sequence file: RDB12132_A9Cib.seq
>08255_12132_A9Ci_Cm_C_F_B04_02_ABI08.ab1 1 CAAGAACGCG GTGGGACCGG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT 61 GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT 121 ATGCTATGAA GCAGCGTATT ACAGTGACAG TTGACAGCGA CAGCTATCAG TTGCTCAAGG 181 CATATATGAT GTCAATATCT CCGGTCTGGT AAGCACAACC ATGCAGAATG AAGCCCGTCG 241 TCTGCGTGCC GAACGCTGGA AAGCGGAAAA TCAGGAAGGG ATGGCTGAGG TCGCCCGGTT 301 TATTGAAATG AACGGCTCTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG 361 TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA 421 TTGACACGCC CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA 481 AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA 541 CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC 601 ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG 661 GCTCCCTTAT ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGA //
Primer: IRES-F Sequence file: RDB12132_A9Cic.seq
>08255_12132_A9Ci_IRES-F_C04_03_ABI08.ab1 1 GGCAGTCCCT TTCATGGTGT TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC CCCCGAACCA 61 CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGTGAGCA 121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA 181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA 241 CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA 301 CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT 361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG 421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA 481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT 541 ACAACTACAA CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG 601 CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC 661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT 721 ACCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT 781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA GAATTCAGGG 841 CCTCTCGAGC CTCTAGAGTT AACATCGAGG GATCAAGCTT ATCGATAATC AACCCTCTGG 901 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT 961 GTGGATACGC TGCTTTAATG CCTTTGGTAT CATGCTATTG CTTCCCGGTA TGGCTTTCAA 1021 TTTTTCTTCT CCTGTATAAA TCCCTGGGTT TGCCTGGTCT CTTATGAGGA GTTGTGGGCC 1081 CCGTTGTCAA GGCCAACCGT TGCGTGATGT GCAACTGTTG TTTGGCTGGA ACGCACCCCC 1141 ACTTGGTTGG GGGCATTTGA CCTACCAGCT TGTTTCTAAG CTTCCTTATC //
Primer: CMV-Forward Sequence file: RDB12132_A9Cid.seq
>08255_12132_A9Ci_CMV-Forward_D04_04_ABI08.ab1 1 AGGGATCCAC CTTCCCGCCC CATTGACGGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG 61 TCTATATAAG CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC 121 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA 181 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA 241 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA 301 AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA 361 GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG 421 AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA 481 AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA 541 GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA 601 GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT 661 TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA 721 AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG 781 CGGCCGCTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAAATTGGA GAAGTGAATT 841 ATATAAATAT AAAGTAGTAA AAATTGAACC ATTAAGGAGT AGCACCCACC AAGGCAAAGA 901 GAAGAGTGGT GCAGAGAGAA AAAAGAGCAG TGGGGAATAG GAGCTTTTGT TTCCTTGGGT 961 TTCTTTGGGA GCAGCAGGGA AGCACTATGG GGCGGCAGCG TTCAATGGAC GCTGACGGTA 1021 CAAGGCCCAG AACAATTTAT TGTTCTGGTA TTAGTTGCAG CCAGCAGAAA CAAATTTTGC 1081 CTGGAAGGGG CTATATTTGG AAGGGCCGCA ACAGCATCCT GTTTGCAAAC TCTCAACAGT 1141 CTGGGGGCAT TCAAGCAGCT CCCAGGCCAA AGAAATTCCT TGGCCTTGTT GTAGGAAGAG 1201 ATATGATGCT //
Primer: BGH_rev2 Sequence file: RDB12132_A9Cie.seq
>08255_12132_A9Ci_BGH_rev2_E04_05_ABI08.ab1 1 TTCGTAGGCG GGGTTCAGGA GCACGGGGGA GGGGCAAACA ACAGATGGCT GGCAACTAGA 61 AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG 121 ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA 181 CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG 241 AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT 301 CTCGGGCCAT GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT 361 CCGGGTCATC CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT 421 TGGCTTCTTC TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA 481 GTGGATATCT GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG 541 TGTGGTAGAT CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA 601 GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC 661 TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC 721 AGGCACAATC AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA 781 GGTCTCGACG GTATCGATGC GGGGAGGCGG CCCAAAGGGA GATCCGACTC GTCTGAGGGC 841 GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG 901 CTGGATTGAG GGCCGAAAGG GACGTAGCAA AAAGGACGTC CCGCGCAGAA TCCAGGTGGC 961 AACACAGGGC GAGCAGCCAT GGGAAAGGAC GTCAGCTTCC CGACAACACC ACGGAAATTG 1021 TCAGTGCCCA ACAGCCGAGC CCCTGGTCCA GCAGCGGGGC CAAGGCAGCG GGCGATGAAG 1081 TTCCGCCGTG GGCAATAGGG AGGGAAAAGG GCGAAGGTTC CGAAGAGCTG GACCAGGTGT 1141 GCCAATGCCC CAACCAGTGG GGATTGCCGT CCAGACA //
Primer: SV40pro_F Sequence file: RDB12132_A9Cif.seq
>08255_12132_A9Ci_SV40pro_F_F04_06_ABI08.ab1 1 CCTTCGGGGC CCCGGACATT CAGGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 61 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA 121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA 181 TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG 241 AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG 301 TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA 361 ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG 421 TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC 481 CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG 541 AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 721 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 781 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 841 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 901 ACAACATACG AGCCGGAAGC ATAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 961 CACATTAATT GCGTTGCGCT CACTGCCCCG CTTTCCAGTC GGGAAAACCT GTCGTGCCAG 1021 CTGGCAATTA ATGAATTCGG GCCCAACGCG CCGGGGAGAG GCGGTTTGCG TAATTGGGGC 1081 GCTCTTTCGC TTCCTTCGCT CACTGAACTC GCTGCGGCTT CGGTCGCTCG GCTGCGGCGA 1141 GCGGGTATCA GGCCTCCACT CAAAGGGCCG GTTTAAATAC CGCGGTTTAA TTCTCCAACC 1201 A //
Primer: env_F Sequence file: RDB12132_A9Cig.seq
>08255_12132_A9Ci_env_F_G04_07_ABI08.ab1 1 GCGCGGGGGC CGGGATCCAT TCGATTAGTG AACGGATCTA CAAATGGCAG TATTCATCCA 61 CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 121 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 181 TCGGGTTTAT TACAGGGACA GCAGAAATTC ACTTTGAATT CTAGCTCGAG ATCGGGAGAT 241 CTGGCCTCCG CGCCGGGTTT TGGCGCCCCC CGCGGGCGCC CCCCTCCTCA CGGCGAGCGC 301 TGCCACGTCA GACGAAGGGC GCAGCGAGCG TCCTGATCCT TCCGCCCGGA CGCTCAGGAC 361 AGCGGCCCGC TGCTCATAAG ACTCGGCCTT AGAACCCCAG TATCAGCAGA AGGACATTTT 421 AGGACGGGAC TTGGGTGACT CTAGGGCACT GGTTTTCTTT CCAGAGAGCG GAACAGGCGA 481 GGAAAAGTAG TCCCTTCTCG GCGATTCTGC GGAGGGATCT CCGTGGGGCG GTGAACGCCG 541 ATGATTATAT AAGGACGCGC CGGGTGTGGC ACAGCTAGTT CCGTCGCAGC CGGGATTTGG 601 GTCGCGGTTC TTGTTTGTGG ATCGCTGTGA TCGTCACTTG GTGAGTAGCG GGCTGCTGGG 661 CTGGCCGGGG CTTTCGTGGC CGCCGGGCCG CTCGGTGGGA CGGAAGCGTG TGGAGAGACC 721 GCCAAGGGCT GTAGTCTGGG TCCGCGAGCA AGGTTGCCCT GAACTGGGGG TTGGGGGGAG 781 CGCAGCAAAA TGGGCGGCTG TTCCCGAGTC TTGAATGGAA GACGCTTGTG AGGCGGGCTG 841 TGAGGTCGTT GAAACAAGGT GGGGGGCATG GTGGGCGGCA AGAACCCAAG GTCTTGAGCC 901 TTCGCTAATG CGGGGAAAGC TCTTATTCGG GTGAGATGGG CTGGGGGCAC CATCTGGGGA 961 CCCTGACGTG AAGCTTTGTC ACCTGACTGG AAAACTCGTT TGTCGTCTGT GCCGGGGGGC 1021 GGCAGTTAAT GGGCGTGCGT TGGGCAATGC CACCCGGTAA CCTTGGGGAA GCGCGCGGCC 1081 CTTCGGTCGT GGTCGT //