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pcDNA3.1-N-FlagC-humanCAPN3(p94) (#RDB08250)

Expression vector of human calpain 3, (p94), FLAG-tagged

Clone info. Expression vector of human calpain 3, (p94), FLAG-tagged.
Vector backbone pcDNA3.1-N-Flag-C (Plasmid)
Selectable markers Amp^r
Gene/insert name Human calpain 3, (p94) cDNA
Depositor|Developer Sorimachi, Hiroyuki | Ono, Yasuko | Hata, Shoji |
 

Distribution information

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Terms and conditions for distribution This material is provided with written consent of DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(J. Biol. Chem., 285, 22986-22998(2010).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08250 pcDNA3.1-N-FlagC-humanCAPN3(p94) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-N-FlagC-humanCAPN3(p94) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08250).

Reference section:

Ono, Y., Ojima, K., Torii, F., Takaya, E., Doi, N., Nakagawa, K., Hata, S., Abe, K., Sorimachi, H., Skeletal muscle-specific calpain is an intracellular Na+-dependent protease. J. Biol. Chem., 285, 22986-22998(2010). PMID 20460380. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08250_A8Avp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB08250_A8Ava.seq check
>08250_08250_A8Av_CMV-Forward_G01_07_ABI08.ab1
    1 GGGACCCAAC TCCCGGCCCC CATTGGACGC AAAATGGGGG CGGTAGGCGT GTACGGTGGG
   61 GGAGGTCTAT TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA
  121 TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT
  181 TACCATGGGT GATTACAAGG ATGACGACGA TAAGGTACCA GGATCCATGC CGACCGTCAT
  241 TAGCGCATCT GTGGCTCCAA GGACAGCGGC TGAGCCCCGG TCCCCAGGGC CAGTTCCTCA
  301 CCCGGCCCAG AGCAAGGCCA CTGAGGCTGG GGGTGGAAAC CCAAGTGGCA TCTATTCAGC
  361 CATCATCAGC CGCAATTTTC CTATTATCGG AGTGAAAGAG AAGACATTCG AGCAACTTCA
  421 CAAGAAATGT CTAGAAAAGA AAGTTCTTTA TGTGGACCCT GAGTTCCCAC CGGATGAGAC
  481 CTCTCTCTTT TATAGCCAGA AGTTCCCCAT CCAGTTCGTC TGGAAGAGAC CTCCGGAAAT
  541 TTGCGAGAAT CCCCGATTTA TCATTGATGG AGCCAACAGA ACTGACATCT GTCAAGGAGA
  601 GCTAGGGGAC TGCTGGTTTC TCGCAGCCAT TGCCTGCCTG ACCCTGAACC AGCACCTTCT
  661 TTTCCGAGTC ATACCCCATG ATCAAAGTTT CATCGAAAAA CTACGCAGGG ATCTTCCACT
  721 TCCAGTTCTG GCGCTATGGA GAGTGGGTGG ACGGTGGTTA TAGATGACTG CCTGCCAAAC
  781 GTACAACAAT CAACTGGTTT TCACCAAGTC CAAACCACCG CAATGAGTTC TGGAGTGCTC
  841 TGCTGGAGAA GGCTTATGCT AAGGCTCCAT GGTTCCTACG GAAAGCTCTG AAAAGGTGGG
  901 GAACACCACA GAGGCCCATG GGAGGACTTT CACAGGAGGG GGTGGCAGAG TTTTTTTGAG
  961 ATCAGGGATG CTCCTTAGTG AACATGTACC AAGATCATGG AAGAAAGCCC ATCGAGAAGA
 1021 GGCTCCCTCA TGGGGCTGCC TCCAATTGAA TGAATGGGTA CCAACAATGA ACCTAATGGG
 1081 AACCTTCTCC TTCTGGTTCT GGAACCAATG GGGGAAGGTG AATTGCACGG GAATGGTTAG
 1141 GAAATAATGG AATACTCCCA CTTGCCTCCA AGGAACCTCT CTAGAAACCC CTCGCTAGAA
1201 TCTCGTC
//
Primer: BGH_rev2
Sequence file: RDB08250_A8Avb.seq check
>08250_08250_A8Av_BGH_rev2_F02_17_ABI24.ab1
    1 CATGCAGGTT TAGGGAAGGC ACGGGGGAGG GGCAAACAAC AGATGGCTGG CAACTAGAAG
   61 GCACAGTCGA GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCCACT
  121 GTGCTGGATT CGAGGGCTGC AGGAATTCCT TTTTTTTTTT TTTTTTTTTT GACCAGCCAT
  181 ACACATGCCT TTATTTAGAT CAGCTTTTTT CAAAATGCAG CCAAACTTAT GAGTTGGACA
  241 GCCCAAAGTA ACCAACCCTA TTCCACTGAG TTAGTTTACC CCACAGCAGT AGAACCCAGT
  301 GCTGGTTTGG TTCCTGGCCC ATGGTGGGAC AGCGTGAAGG TGATGGAGGG CTCTAGCACA
  361 AGGAGGTGCT GAGTGCCACC GGCAGGTGCT TCTGCAGACA GCCTAGAGCA AGGTAAGCAG
  421 GAGCACTCGC TTCAGAACCG AGGCGGCTCG GACCAGAGGC AGGCACTTTC CTCCACATGG
  481 CAAAGGGACA AGGGTTTGTT CTTCCTACCT CATTGCTTTA CTAAAGGGTC AGGCTAGGCA
  541 TGACCGATCA AGGATGGGTA GGGGGTAAAA TGGAGGAGGA ACATGACTGA TGAGGTGCCT
  601 GGAAGCCTGT AGGGGTGTAG CTGCTGGGTC CTTTGAGGTA AATGGCTTTG GAAATAGAGG
  661 GTGAAACTGA AATCCTGAGT GATCCTGCAT GGCTTTGGAT GAGGCCAGCC TGGTTCAGGC
  721 ATACATGGTG AGCTGCAGCC ACTCCAGAAC GTTGAGCTTG ATGATACCAT CTCCATCCTT
  781 GTCAAATGCA TGAAAAGCTC TGAACATGCC CTCCAGCCTA ACGAAGCAGC AGATGAAACT
  841 GTCAAAGTCG ATGTTCATGT GTTTGTCTGC GTACCGCATG GTAATGATGT CATAGAGCTG
  901 GTTGTTGAGG TGGAAATCCT GCGTCGTTGA CTGCCATTTC GCATCTCGTA GCCTGTTTGA
  961 TGGTGCCGGG ACCTGGTCCT GTGTCATAGT GTTTGAAATT TTTTCCTGCC AGGCCCTTTA
 1021 TCCTTCACCA GGAGGTGGTG GAACTTCCTT GCCAAGTTGA GCCTTCCCAG AGTCCATCTT
1081 GTAATCCATG GAATCGCGCC AAACTCCAA
//
Primer: SV40pro_F
Sequence file: RDB08250_A8Avc.seq check
>08250_08250_A8Av_SV40pro_F_H01_08_ABI08.ab1
    1 ACGCTGCGCC TGGGACCTAT TCAGAAGGTA GTGGAGGAGG CTTTTTTGGG AGGGCCTAGG
   61 CTTTTGCAAA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA
  121 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT
  181 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC
  241 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC
  301 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC
  361 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG
  421 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC
  481 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC
  541 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT
  601 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGGCT
  661 CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC
  721 GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGGCTGGGTG
  781 TGGCGGACCG CTATCAGGAC ATAGGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC
  841 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGG ATTCGCAGCG
  901 CATCGCCTTC TATCGCCTTT CTTGACGAGT TCTTCTGAGC GGGGACTCTG GGGGTTCGAA
  961 ATGACCGGAC CAAGCGACGC CCAAACCTGC CATCACGAGA TTTCGATTCC ACCCGCCGCC
 1021 TTTCTATGAA AAGGTTGGGG CTTCGGAATC GTTTTTCCGG GACGGCCGGC TGGATGATTC
 1081 CTTCAAGCGC GGGGATCTCA TTGGCCTGGA GGTCTCCGCT ACCCAACCTT GGATTTATTG
1141 GCAGCTTTAA TAATGGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ono, Y., Skeletal muscle-specific calpain is an intracellular Na+-dependent protease. J. Biol. Chem., 285, 22986-22998(2010). PMID 20460380. [link to RRC of NBRP]