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CS-CDF-CG-PRE-RfA

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by CMV promoter.

Catalog number RDB08240
Resource name CS-CDF-CG-PRE-RfA
Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by CMV promoter.
Comment Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
             
            Sequence RDB08240z.seq
            Remarks, protocol and/or map (pdf) RDB08240.pdf
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering Please visit Ordering instruction.[link] 
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08240 CS-CDF-CG-PRE-RfA DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08240_A5Dhp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CSII-CMV5'
            Sequence file: RDB08240_A5Dha.seq check
            >08240_08240_A5Dh_CSII-CMV5'_D03_12.ab1
                1 ACAGGCCGCT ACGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT
               61 GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA
              121 GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA
              181 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG
              241 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA
              301 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT
              361 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA
              421 GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT
              481 CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA
              541 GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC
              601 CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA
              661 CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG
              721 CATGGACGAG CTGTACAAGT CCGGACTCAG ATCCTACTAG TAGGATCTCG AAACATCGAG
              781 GGATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA
              841 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC
              901 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTTGCTG
              961 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGCAACGTGG CGTGTGGTGC ACTGTGTTTG
             1021 CTGACGCACC CCCCACTGAT GGGGCATTGC ACACTGTCAG CTCTTTCGGA CCTTTCGCTT
            1081 TCCCACCGTA TGCACGCGAA CTCATCGCGC CTGTCCTTGC CCGCCTGT
            //
            Primer: Cm_M_R
            Sequence file: RDB08240_A5Dhb.seq check
            >08240_08240_A5Dh_Cm_M_R_E03_15.ab1
                1 CATGGAGCGA CCTATCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAATTCCGG
               61 ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT
              121 TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA
              181 TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC
              241 GGTGGTATAT CCAGTGATTT TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA
              301 CGGATCCTAA CTCAAAATCC ACACATTATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG
              361 GGTGCCTAAT GCGGCCGCCA TAGTGACTGG ATATGTTGTG TTTTACAGTA TTATGTAGTC
              421 TGTTTTTTAT GCAAAATCTA ATTTAATATA TTGATATTTA TATCATTTTA CGTTTCTCGT
              481 TCAGCTTTTT TGTACAAACT TGTGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC
              541 AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT
              601 CTTAAAGGTA CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC
              661 CAGGCACAAT CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT
              721 AGGTCTCGAC GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG
              781 CGAAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCCGGCAG CAGGCCGCGG GAAGGAAGGT
              841 CCGCTGGATT GAGGGCCGAA GGGACGTAGC AGAGGACGTC CCGCGCAGAA TCCAGGTGGC
              901 ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACACACCACG GGAATTGTCA
              961 GTGCCACAGC GAGCCCCTGT CAGCAGCGGG CAGCAGCGGC GATGAGTCGT CGTGCCATAG
             1021 GAGGGGAAGC GAAGTCCGGA CGGAACTGAC GGTGATGCAT GCCTATCATG GGGATGCGTT
            1081 CAGCTAAACC AGTGCTGAT
            //
            Primer: BGH_rev
            Sequence file: RDB08240_A5Dhc.seq check
            >08240_08240_A5Dh_BGH_rev_F03_18.ab1
                1 GTTAAGGGGG GCCCTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA
               61 GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA
              121 TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC
              181 TAACCAGAGA GACCCAGTAC AGTCCGGATC ACCACTTTGT ACAAGAAAGC TGAACGAGAA
              241 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA
              301 TAATACTGTA AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT
              361 AAGGGAGCCT GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT
              421 TTCGCGGTGG CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT
              481 ATCGGTGGTC ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG
              541 GGAGACTTTA TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG
              601 CGTGTCAATA ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA
              661 GGTGTAAACC TTAAACTGCA TTTCACCAGC CCCTGTTCTC GTCAGCAAAA GAGCCGTTCA
              721 TTTCAATAAA CCGGGCGACC TCAGCCATCC CTTCCTGATT TTCCGCTTTC CAGCGTTCGG
              781 CACGCAGACG ACGGGCTTCA TTCTGCATGG TTGTGCTTAC CAGACCGGAG ATATTGACAT
              841 CATATATGCC TTGAGCAACT GATAGCTGTC GCTGTCAACT GTCACTGTAA TACGCTGCTT
              901 CATAGCATAC CTCTTTTTGA CATACTTCGG GGTATACATA TCAGTATATA TTCTTATACC
              961 GCAAAAATCA GCGCGCAAAT ACGCATACTG TTATCTGGCT TTTAGTAAGC CGGGATCCAC
             1021 GCGTTTACGC CCCGCCCTGC CACTCATCGC AGTACTGTGT AAATTCATTT AAGCATTCCT
             1081 GGCCGACATG GCAAGCCATC AACAAACGGC ATGAATGGAC TGGATTCGGT CAGCGCATCA
            1141 GCACCTTGGC TCGCTGCTAT CAATGTGG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.04.10

            GNP_filter3_RDBDEP_html_230405.pl