Resource data sheet
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CS-CDF-CG-PRE-RfA

Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by CMV promoter.

Catalog number RDB08240
Resource name CS-CDF-CG-PRE-RfA
Clone info. Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by CMV promoter.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Selectable markers Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
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Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


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RDB08240 CS-CDF-CG-PRE-RfA DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08240z.seq from Depositor
Electronic file PDF RDB08240.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08240_A5Dhp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CSII-CMV5' RDB08240_A5Dha.seq
>08240_08240_A5Dh_CSII-CMV5'_D03_12.ab1
    1 ACAGGCCGCT ACGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT
   61 GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA
  121 GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA
  181 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG
  241 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA
  301 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT
  361 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA
  421 GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT
  481 CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA
  541 GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC
  601 CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA
  661 CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG
  721 CATGGACGAG CTGTACAAGT CCGGACTCAG ATCCTACTAG TAGGATCTCG AAACATCGAG
  781 GGATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA
  841 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC
  901 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTTGCTG
  961 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGCAACGTGG CGTGTGGTGC ACTGTGTTTG
 1021 CTGACGCACC CCCCACTGAT GGGGCATTGC ACACTGTCAG CTCTTTCGGA CCTTTCGCTT
1081 TCCCACCGTA TGCACGCGAA CTCATCGCGC CTGTCCTTGC CCGCCTGT
//
Primer: Cm_M_R RDB08240_A5Dhb.seq
>08240_08240_A5Dh_Cm_M_R_E03_15.ab1
    1 CATGGAGCGA CCTATCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAATTCCGG
   61 ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT
  121 TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA
  181 TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC
  241 GGTGGTATAT CCAGTGATTT TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA
  301 CGGATCCTAA CTCAAAATCC ACACATTATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG
  361 GGTGCCTAAT GCGGCCGCCA TAGTGACTGG ATATGTTGTG TTTTACAGTA TTATGTAGTC
  421 TGTTTTTTAT GCAAAATCTA ATTTAATATA TTGATATTTA TATCATTTTA CGTTTCTCGT
  481 TCAGCTTTTT TGTACAAACT TGTGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC
  541 AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT
  601 CTTAAAGGTA CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC
  661 CAGGCACAAT CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT
  721 AGGTCTCGAC GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG
  781 CGAAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCCGGCAG CAGGCCGCGG GAAGGAAGGT
  841 CCGCTGGATT GAGGGCCGAA GGGACGTAGC AGAGGACGTC CCGCGCAGAA TCCAGGTGGC
  901 ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACACACCACG GGAATTGTCA
  961 GTGCCACAGC GAGCCCCTGT CAGCAGCGGG CAGCAGCGGC GATGAGTCGT CGTGCCATAG
 1021 GAGGGGAAGC GAAGTCCGGA CGGAACTGAC GGTGATGCAT GCCTATCATG GGGATGCGTT
1081 CAGCTAAACC AGTGCTGAT
//
Primer: BGH_rev RDB08240_A5Dhc.seq
>08240_08240_A5Dh_BGH_rev_F03_18.ab1
    1 GTTAAGGGGG GCCCTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA
   61 GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA
  121 TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC
  181 TAACCAGAGA GACCCAGTAC AGTCCGGATC ACCACTTTGT ACAAGAAAGC TGAACGAGAA
  241 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA
  301 TAATACTGTA AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT
  361 AAGGGAGCCT GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT
  421 TTCGCGGTGG CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT
  481 ATCGGTGGTC ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG
  541 GGAGACTTTA TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG
  601 CGTGTCAATA ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA
  661 GGTGTAAACC TTAAACTGCA TTTCACCAGC CCCTGTTCTC GTCAGCAAAA GAGCCGTTCA
  721 TTTCAATAAA CCGGGCGACC TCAGCCATCC CTTCCTGATT TTCCGCTTTC CAGCGTTCGG
  781 CACGCAGACG ACGGGCTTCA TTCTGCATGG TTGTGCTTAC CAGACCGGAG ATATTGACAT
  841 CATATATGCC TTGAGCAACT GATAGCTGTC GCTGTCAACT GTCACTGTAA TACGCTGCTT
  901 CATAGCATAC CTCTTTTTGA CATACTTCGG GGTATACATA TCAGTATATA TTCTTATACC
  961 GCAAAAATCA GCGCGCAAAT ACGCATACTG TTATCTGGCT TTTAGTAAGC CGGGATCCAC
 1021 GCGTTTACGC CCCGCCCTGC CACTCATCGC AGTACTGTGT AAATTCATTT AAGCATTCCT
 1081 GGCCGACATG GCAAGCCATC AACAAACGGC ATGAATGGAC TGGATTCGGT CAGCGCATCA
1141 GCACCTTGGC TCGCTGCTAT CAATGTGG
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.10.09

GNP_filter3_RDBDEP_html_180823.pl