Bacterial expression vector of E. coli pheA, feedback resistance allele
| Alternative name | pET21-pheA^fbr1 (S330P) |
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| Clone info. | Bacterial expression vector of E. coli pheA, fused chorismate mutase P and prephenate dehydratase (Genpept: AAC75648), feedback resistance allele (S330P). |
| Comment | Apmlicon from genome into NdeI- XhoI site of pET21a. F primer :CCAACCATATGACATCGGAAAACCCGTTAC, R primer :CACTCGAGTCAGGTTGGATCAACAGGCAC. |
| Vector backbone | pET21a (Plasmid) |
| Selectable markers | Amp^r |
| Gene/insert name | E. coli pheA^fbr genomic DNA |
| Depositor|Developer | Koma, Daisuke | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment MTA, for use for not-for-profit academic purpose [Word Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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| Terms and conditions for distribution | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
[open/close]| 必要書類 | 提供依頼書 提供同意書 (MTA, 非営利学術目的用)[Word 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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| MTAに書く使用条件 | 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 |
| Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
|---|---|---|---|---|
| RDB08227 | pARO12 | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
| The pARO12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08227). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
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