Resource data sheet
Prev.

pGEXMKK6

Bacterial expression vector of human MKK6 cDNA, GST-tagged

Catalog number RDB08212
Resource name pGEXMKK6
Clone info. Bacterial expression vector of human MKK6 cDNA, GST-tagged. cDNA fragment 289nt to 1491nt of NM_002758.3 (cds, 289..1293) is cloned.
Known difference : T to G substitution at 292nt of NM_002758.3 (Ser to Ala).
Vector backbone pGEXNX (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human MKK6 cDNA
Depositor Sudo, Tatsuhiko |
 
Other clones in our bank human MAP2K6 (NCBI Gene 5608) |

Distribution information

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RDB08212 pGEXMKK6 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content MAPK p38 inhibitor screening (English text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08212_A7Ktp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 RDB08212_A7Kta.seq
>08212_08212_A7Kt_1_pGEX-F2_B11_05_ABI24.ab1
    1 CAGTAGATGT TTTAAAAACG TATTGAGCTA TCCCACAAAT TGATAAGTAC TTGAAATCCA
   61 GCAAGTATAT AGCATGGCCT TTGCAGGGCT GGCAAGCCAC GTTTGGTGGT GGCGACCATC
  121 CTCCAAAATC GGATCTGATC GAAGGTCGTG GGATCCCCGC CATGGCTCAG TCGAAAGGCA
  181 AGAAGCGAAA CCCTGGCCTT AAAATTCCAA AAGAAGCATT TGAACAACCT CAGACCAGTT
  241 CCACACCACC TCGAGATTTA GACTCCAAGG CTTGCATTTC TATTGGAAAT CAGAACTTTG
  301 AGGTGAAGGC AGATGACCTG GAGCCTATAA TGGAACTGGG ACGAGGTGCG TACGGGGTGG
  361 TGGAGAAGAT GCGGCACGTG CCCAGCGGGC AGATCATGGC AGTGAAGCGG ATCCGAGCCA
  421 CAGTAAATAG CCAGGAACAG AAACGGCTAC TGATGGATTT GGATATTTCC ATGAGGACGG
  481 TGGACTGTCC ATTCACTGTC ACCTTTTATG GCGCACTGTT TCGGGAGGGT GATGTGTGGA
  541 TCTGCATGGA GCTCATGGAT ACATCACTAG ATAAATTCTA CAAACAAGTT ATTGATAAAG
  601 GCCAGACAAT TCCAGAGGAC ATCTTAGGGA AAATAGCAGT TTCTATTGTA AAAGCATTAG
  661 AACATTTACA TAGTAAGCTG TCTGTCATTC ACAGAGACGT CAAGCCTTCT AATGTACTCA
  721 TCAATGCTCT CGGTCAAGTG AAGATGTGCG ATTTTGGAAT CAGTGGCTAC TTGGTGGACT
  781 CTGTTGCTAA AACAATTGAT GCAGGTTGCA AACCATACAT GGCCCCCTGA AAGAATAAAC
  841 CCAGAGCTCA ACCAGAAGGG ATACAGTGTG AAGTCTGACA TTTGGAGTCT GGGCATCACG
  901 ATGATTGAGT TGGCCATCCT TCGATTTCCC TATGATTCAT GGGGGAAACT CCATTTCAGC
  961 AGCTCAAACA GGTGGTAGAG GGAGCCATCG CCACAACTCC CAGCAGACAA GTTCTCTGCA
 1021 GAGTTTTGTT GACTTTACCC TCACAGTGGC TAAAGAGACT TTCCCAAGAC GACTACATAC
1081 CTCAGAGCTT ATGCACATCC ATTTTTTTC
//
Primer: pJB8-Forward RDB08212_A7Ktb.seq
>08212_08212_A7Kt_1_pJB8-Forward_C11_08_ABI24.ab1
    1 TAATCGCTCG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC
   61 CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA
  121 GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG ATCGTCAGTC
  181 AGTCACGATG AATTTCTAGA CAGTTCATTC TATACTATAG ATTCCAAGGC CCCCTTAAGA
  241 GGGGGAGTAA AAAGAAAATT GGGAGAGAAA ACCCTCTGAG AGGCAGGGTT AAATTATCTC
  301 AAAGATGACT TTCTATTGAT GCTGTAGTGA ACTTGCTTCA CCCCGAAACC CACCAATCCA
  361 CAGTAGGGTC AACCGATTAA GTCCACTGCT TTTTAGTCTC CAAGAATCAG TTTTACAAAA
  421 GATGCCACAT CTGTTCCTTT GGATTCATGT AGGGTGAAAA ATGGATGTTG CATTAGCTCT
  481 GGGTATGTAG GCCGTTCTTT GGAATTCTTC TTTAAGCACT GTGAGGTAAA GTCAACAAAC
  541 TCTGCAGAGA ACTTGTCTGC TGGGAGTTGT GGCGATGGCT CCTCTACCAC CTGTTTGAGC
  601 TGCTGAAATG GAGTTCCCCA TGAATCATAG GGAAATCGAA GGATGGCCAA CTCAATCATC
  661 GTGATGCCCA GACTCCAAAT GTCAGACTTC ACACTGTATC CCTTCTGGTT GAGCTCTGGG
  721 TTTATTCTTT CAGGGGCCAT GTATGGTTTG CAACCTGCAT CAATTGTTTT AGCAACAGAG
  781 TCCACCAAGT AGCCACTGAT TCCAAAATCG CACATCTTCA CTTGACCGAG AGCATTGATG
  841 AGTACATTAG AAGGCTTGAC GTCTCTGTGA ATGACAGACA GCTTACTATG TAAATGTTCT
  901 AATGCTTTTA CAATAGAAAC TGCTATTTTC CCTAAGATGT CCTCTGGAAT TGTCTGGCCT
  961 TTATCAATAA CTTGTTTGTA GAAATTTATC TAGTGATGTA TCCATGAGCT CCATGCAGAT
 1021 CCCACACATC ACCCTCCCCG AAAACAGGTG CCGCCATAAA ATGGTGACAG CTGAATGACA
1081 GGTTCAACGG ATCCTCATGG AAAATTATTC CAAAAATTCC CAT
//
Primer: pGEX-ptac_up-F RDB08212_A7Ktc.seq
>08212_08212_A7Kt_1_pGEX-ptac_up-F_D11_11_ABI24.ab1
    1 ATTAGCCGCT CATACGGTTC TGGCAATATT CTGAAATGAG CTGTTGACAA TTAATCATCG
   61 GCTCGTATAA TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGTATTCAT
  121 GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT
  181 GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG
  241 GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG
  301 TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT
  361 GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA
  421 TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA
  481 TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC
  541 ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT
  601 TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA
  661 ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG
  721 GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATCT
  781 GATCGAAGGT CGTGGGATCC CCGCCATGGC TCAGTCGAAA GGCAAGAAGC GAAACCCTGG
  841 CCTTAAAATT CCAAAAGAAG CATTTGAACA ACCTCAGACC AGTTCCACAC CACCTCGAGA
  901 TTTAGACTCC AAGGCTTGCA TTTCTATTGG AAATCAGAAC TTTGAGGTGA AAGGCAGATG
  961 ACCTGGAGCC CTATAATGGA AACTGGGACG AGGTGCGTAC GGGGTGGTGG AGAAGATGCG
 1021 GGCACGGTGC CCAGCGGCAG ATCATGGCCA GTTGAGGCGG ATCCCGAGCT ACAGTAAATA
1081 GTCAGCAACC AGAAACGGCT ACTGGATGGG
//
Primer: M13_-40 RDB08212_A7Ktd.seq
>08212_08212_A7Kt_1_M13_-40_E11_14_ABI24.ab1
    1 CGGCGAGTAT CGTATCATGG TCATAGCTGT TTCCTGTGTG AATTGTTATC CGCTCACAAT
   61 TCCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA
  121 GCTAACTTAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
  181 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC
  241 AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG
  301 CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT
  361 TTGATGGTGG TTAACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT
  421 ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC
  481 GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC
  541 ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA
  601 ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA
  661 CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG
  721 CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG
  781 ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG
  841 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC
  901 GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC
  961 AGTTGATCGG CGCGAGATTT AAATCGCCCG CGACATTTGC GACGGGCGCG TGCAGGGCCA
 1021 GGACTGCAGG TGGCACGCCC AATCAGCCAA CCGACTGTTT GCCGCCCAGT TGATTGTGCA
1081 ACGCGATGGG ATGTAATTCC AGCTCCGACC ATTCG
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.11.21

GNP_filter3_RDBDEP_html_181012.pl