Resource data sheet
Prev.

pcDNA3Flag-p38AF

Expression vector of mouse p38alpha MAPK cDNA, constitutive inactive form, FLAG-tagged

Catalog number RDB08161
Resource name pcDNA3Flag-p38AF
Alternative name pcDNAM2p38K53R
Clone info. Expression vector of mouse p38alpha MAPK cDNA, constitutive inactive form, FLAG-tagged.
Amino acids TGY were substituted by AGF.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4
Selectable markers Amp^r
Gene/insert name Mouse p38alpha MAPK cDNA
Depositor Sudo, Tatsuhiko |
 
Other clones in our bank mouse Mapk14 (NCBI Gene 26416) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Research purpose only.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08161 pcDNA3Flag-p38AF DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content MAPK p38 inhibitor screening (English text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08161_A4E9.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB08161_A4E9a.seq
>08161_08161_A4E9_BGHrev_F04_16.ab1
    1 GACTGAGCCA TTTTAGGTGA ACTATAGAAT AGGGCCCTCT AGACAGTGAA GTGAGATAGA
   61 CAGAACAGAA ACCAGGTGCT CAGGACTCCA TTTCTTCTTG GTCAAGGGGT GGTGGCACAA
  121 AGCTGATGAC TTCATCATAG GTCAGGCTCT TCCACTCATC TATGAGAAGG TCCCTGCTTT
  181 CAAAGGACTG GTCATAAGGG TCAGCAACAG GCTCATCATC AGGGTCGTGG TACTGAGCAA
  241 AGTAGGCATG CGCAAGAGCT TGGGCTGCTG TGATCCTCTT ATCTGAGTCC AAAACGAGCA
  301 TCTTCTCCAG TAGGTCGACA GCCAGGGGAT TGGCACCAAT AAATACATTT GCGAAGTTCA
  361 TCTTCGGCAT CTGGGCCAGA GACTGAATGT AGTTTCTTGC AGACTCTGAG GAGATTTTCT
  421 TCAGAAGCTC AGCCCCTGGG GTTCCAACGA GTCTTAAAAT GAGCTTCAAC TGATCAATAT
  481 GGTCTGTACC AGGAAACAAC GTTCTTCCGG TCAACAGCTC AGCCATGATG CAGCCCACGG
  541 ACCAAATATC CACTGTCTGG TTATAGTGCA TCCAATTCAG CATGATCTCT GGGGCTCGGT
  601 ACCACCTGGT AGCCACGAAG CCTGCCATCT CATCATCAGT GTGCCGAGCC AGCCCAAAAT
  661 CCAGAATCTT GAGCTCACAG TCTTCGTTCA CAGCTAGGTT GCTGGGCTTT AGGTCCCTGT
  721 GAATTATGTC AGCCGAATGT ATATACTTCA GCCCTCGGAG GATCTGGTAG ATGAGAAACT
  781 GAACGTGGTC GTCGGTCAGC TTCTGGCACT TCACGATGTT GTTCAGGTCC GCCCCCATGA
  841 GATGGGTCAC CAGGTACACG TCATTGAATT CCTCCAGTGA CCTTGCGGGT GTGAACACAT
  901 CCAACAGACC AATCACATTT TCGTGTTTCA TGTGCTTCAG CAGACGCAAC TCTCGGTAGG
  961 TCCTTTTGGC GTGAATGATG GACTGAAACG GTCTCGACAG CTTCTTAACT GCCACACGAT
 1021 GCCCCGTCTT TGTATCAAAA GCAGCACACA CCGAGCCCAT AGGCGCCCGA GCCCACCGGG
 1081 GGACAGGGTT CTGGGTATCG TTCCGGGCAC TTCCAGATTG ATCCTGGATT CAGCTCCTGA
1141 CCGGTTAGAA ACGGGTGGGG TCATC
//
Primer: BGH_rev RDB08161_A4E9b.seq
>08161_08161_A4E9_CMV-Foward_E04_13.ab1
    1 CGTACAACTC CGCGCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTTGCC ACCATGGACT ACAAGGACGA CGACGATAAA
  181 TCGATGTCGC AGGAGAGGCC CACGTTCTAC CGGCAGGAGC TGAACAAGAC CATCTGGGAG
  241 GTGCCCGAAC GATACCAGAA CCTGTCCCCG GTGGGCTCGG GCGCCTATGG CTCGGTGTGT
  301 GCTGCTTTTG ATACAAAGAC GGGGCATCGT GTGGCAGTTA AGAAGCTGTC GAGACCGTTT
  361 CAGTCCATCA TTCACGCCAA AAGGACCTAC CGAGAGTTGC GTCTGCTGAA GCACATGAAA
  421 CACGAAAATG TGATTGGTCT GTTGGATGTG TTCACACCCG CAAGGTCACT GGAGGAATTC
  481 AATGACGTGT ACCTGGTGAC CCATCTCATG GGGGCGGACC TGAACAACAT CGTGAAGTGC
  541 CAGAAGCTGA CCGACGACCA CGTTCAGTTT CTCATCTACC AGATCCTCCG AGGGCTGAAG
  601 TATATACATT CGGCTGACAT AATTCACAGG GACCTAAAGC CCAGCAACCT AGCTGTGAAC
  661 GAAGACTGTG AGCTCAAGAT TCTGGATTTT GGGCTGGCTC GGCACACTGA TGATGAGATG
  721 GCAGGCTTCG TGGCTACCAG GTGGTACCGA GCCCCAGAGA TCATGCTGAA TTGGATGCAC
  781 TATAACCAGA CAGTGGATAT TTGGTCCGTG GGCTGCATCA TGGCTGAGCT GTTGACCGGA
  841 AGAACGTTGT TTCCTGGTAC AGACCATATT GATCAGTTGA AGCTCATTTT AAGACTCGTT
  901 GGAACCCCAG GGGCTGAGCT TCTGAAGAAA ATCTCCTCAG AGTCTGCAAG AAACTACATT
  961 CAGTCTCTGG CCCAGATGCC GAAGATGAAC TTCGCAAATG TATTTATTGG TGCCCAATCC
 1021 CCTGGCTGTC GACCTACTGG GAGAAGATGC TCGTTTTGGG ACTCAGATAA GAGGATCACA
 1081 GCAGCCCAAG CTCTTGCGCA TGCCTACCTT GGCTCAGTAC ACGAATCCTT GAATGAATGA
1141 GCCTGGTTTG CCTGAACCCC TTTAGGAAGC
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.11.21

GNP_filter3_RDBDEP_html_181012.pl