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pcDNA3Flag-p38 (#RDB08160)

Expression vector of mouse p38alpha MAPK cDNA, FLAG-tagged

Alternative name pcDNAM2p38
Clone info. Expression vector of mouse p38alpha MAPK cDNA, FLAG-tagged.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Gene/insert name Mouse p38alpha MAPK cDNA
Depositor|Developer Sudo, Tatsuhiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Biochem. Biophys. Res. Commun., 269, 521-525 (2000).) Research purpose only.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem. Biophys. Res. Commun., 269, 521-525 (2000).)。 研究目的に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08160 pcDNA3Flag-p38 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3Flag-p38 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08160).

Reference section:

Sudo, T., Maruyama, M., Osada, H., p62 functions as a p38 MAP kinase regulator. Biochem. Biophys. Res. Commun., 269, 521-525 (2000). PMID 10708586. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08160_A4E9.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB08160_A4E9a.seq check
>08160_08160_A4E9_CMV-Foward_C04_07.ab1
    1 TTGTTCACTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTTGCCA CCATGGACTA CAAGGACGAC GACGATAAAT
  181 CGATGTCGCA GGAGAGGCCC ACGTTCTACC GGCAGGAGCT GAACAAGACC ATCTGGGAGG
  241 TGCCCGAACG ATACCAGAAC CTGTCCCCGG TGGGCTCGGG CGCCTATGGC TCGGTGTGTG
  301 CTGCTTTTGA TACAAAGACG GGGCATCGTG TGGCAGTTAA GAAGCTGTCG AGACCGTTTC
  361 AGTCCATCAT TCACGCCAAA AGGACCTACC GAGAGTTGCG TCTGCTGAAG CACATGAAAC
  421 ACGAAAATGT GATTGGTCTG TTGGATGTGT TCACACCCGC AAGGTCACTG GAGGAATTCA
  481 ATGACGTGTA CCTGGTGACC CATCTCATGG GGGCGGACCT GAACAACATC GTGAAGTGCC
  541 AGAAGCTGAC CGACGACCAC GTTCAGTTTC TCATCTACCA GATCCTCCGA GGGCTGAAGT
  601 ATATACATTC GGCTGACATA ATTCACAGGG ACCTAAAGCC CAGCAACCTA GCTGTGAACG
  661 AAGACTGTGA GCTCAAGATT CTGGATTTTG GGCTGGCTCG GCACACTGAT GATGAGATGA
  721 CAGGCTACGT GGCTACCAGG TGGTACCGAG CCCCAGAGAT CATGCTGAAT TGGATGCACT
  781 ATAACCAGAC AGTGGATATT TGGTCCGTGG GCTGCATCAT GGCTGAGCTG TTGACCGGAA
  841 GAACGTTGTT TCCTGGTACA GACCATATTG ATCAGTTGAA GCTCATTTTA AGACTCGTTG
  901 GAACCCCAGG GGCTGAGCTT CTGAAGAAAA TCTCCTCAGA GTCTGCAAGA AACTACATTC
  961 AGTCTCTGGC CCAGATGCCG AAGATGAACT TCGCAAATGT ATTTATTGGT GCCCAATCCC
 1021 CTGGCTGTCG ACCTACTGGT AGAGATGCTC GTTTTGGACT CAGATAGAGG ATCACAGCAG
 1081 CCCAGCTCTG CGCATGCCTA CTTTGCTCAG TAACCACGAC CCTTGCATGA ATGAGCCTGA
1141 TTGTTGAACC GCCTTTGAAG TGAAAC
//
Primer: BGH_rev
Sequence file: RDB08160_A4E9b.seq check
>08160_08160_A4E9_BGHrev_D04_10.ab1
    1 CCACCCTAAA TTTAGGGTTG GCCCTATAAG AATAAGGGCC CCTCTAGACA GTGAAGTGAG
   61 ATAGACAAGA ACAGAAACCA GGTGCTCAGG ACTCCATTTC TTCTTGGTCA AGGGGTGGTG
  121 GCACAAAGCT GATGACTTCA TCATAGGTCA GGCTCTTCCA CTCATCTATG AGAAGGTCCC
  181 TGCTTTCAAA GGACTGGTCA TAAGGGTCAG CAACAGGCTC ATCATCAGGG TCGTGGTACT
  241 GAGCAAAGTA GGCATGCGCA AGAGCTTGGG CTGCTGTGAT CCTCTTATCT GAGTCCAAAA
  301 CGAGCATCTT CTCCAGTAGG TCGACAGCCA GGGGATTGGC ACCAATAAAT ACATTTGCGA
  361 AGTTCATCTT CGGCATCTGG GCCAGAGACT GAATGTAGTT TCTTGCAGAC TCTGAGGAGA
  421 TTTTCTTCAG AAGCTCAGCC CCTGGGGTTC CAACGAGTCT TAAAATGAGC TTCAACTGAT
  481 CAATATGGTC TGTACCAGGA AACAACGTTC TTCCGGTCAA CAGCTCAGCC ATGATGCAGC
  541 CCACGGACCA AATATCCACT GTCTGGTTAT AGTGCATCCA ATTCAGCATG ATCTCTGGGG
  601 CTCGGTACCA CCTGGTAGCC ACGTAGCCTG TCATCTCATC ATCAGTGTGC CGAGCCAGCC
  661 CAAAATCCAG AATCTTGAGC TCACAGTCTT CGTTCACAGC TAGGTTGCTG GGCTTTAGGT
  721 CCCTGTGAAT TATGTCAGCC GAATGTATAT ACTTCAGCCC TCGGAGGATC TGGTAGATGA
  781 GAAAACTGAA CGTGGTCGTC GGTCAGCTTC TGGCACTTCA CGATGTTTGT TCAGGTCCGC
  841 CCCCCATGAG ATGGGTCACC AGGTACACGT TCATTGAAAT TTCCTCCCAG TGACCTTGCC
  901 GGGTGTGAAC ACATCCCAAC AGACCCAATC ACAATTTTTC GTGGTTTCAT GTGCTTCCAG
  961 CCAGACGCAA CTCTCGGTAG GTCCTTTTGG CCGTGAATGA TGGACTGAAA ACGGTCTCGA
 1021 CAGCTTTCTT AACCTGCCAC CACGATGGCC CCCGTCTTTT GTATCCAAAA AGCAAGCCAC
 1081 ACACCGAAGC CCATAAGGCG CCCCGAAACC CAACCGGGGA AACAGGTTCC TGGTAATCCG
 1141 TTTCCGGGCA CCTCCAGATG GGTCTTGGTT CAGCCTCCTG GCCCGGTAGG ACCGGGGTGG
1201 GGGGCCATCC TCTCC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sudo, T., p62 functions as a p38 MAP kinase regulator. Biochem. Biophys. Res. Commun., 269, 521-525 (2000). PMID 10708586. [link to RRC of NBRP]