Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pRSV-Rev

Rev expressing plasmid vector utilized for lentiviral packaging.

Catalog number RDB08121
Resource name pRSV-Rev
Clone info. Rev expressing plasmid vector utilized for lentiviral packaging.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB08121zzt01.seq compiled by us
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 【追加提供依頼書】受入れ確認書(書式J)
            【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08121 pRSV-Rev DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08121_A3Jtp1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: reverse-2
            Region: RSV pro,Rev protein 5'
            Sequence file: RDB08121_A3Jtb.seq check
            >08121_08121_A3Jt_Reverse2_D01_10.ab1
                1 NNNNNNNNNN NNANTCGNNG TNNGGGCNNG NNNTACGCGT ATCTGANGGG NNTAGGGTGT
               61 GTTTAGGCGA AAAGCGGGGC TTCGGTTGTA CGCGGTTAGG AGTCCCCTCA GGATATAGTA
              121 GTTTCGCTTT TGCATAGGGA GGGGGAAATG TAGTCTTATG CAATACTCTT GTAGTCTTGC
              181 AACATGGTAA CGATGAGTTA GCAACATGCC TTACAAGGAG AGAAAAAGCA CCGTGCATGC
              241 CGATTGGTGG AAGTAAGGTG GTACGATCGT GCCTTATTAG GAAGGCAACA GACGGGTCTG
              301 ACATGGATTG GACGAACCAC TGAATTCCGC ATTGCAGAGA TATTGTATTT AAGTGCCTAG
              361 CTCGATACAA TAAACGCCAT TTGACCATTC ACCACATTGG TGTGCACCTC CAAGCTCGAG
              421 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA
              481 GAAGACACCG GGACCGATCC AGCCTCCCCT CGAAGCTAGT CGATTAGGCA TCTCCTATGG
              541 CAGGAAGAAG CGGAGACAGC GACGAAGACC TCCTCAAGGC AGTCAGACTC ATCAAGTTTC
              601 TCTATCAAAG CAACCCACCT CCCAATCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA
              661 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCCTTAGCA
              721 CTTATCTGGG ACGATCTGCG GAGCCTGTGC CTCTTCAGCT ACCACCGCTN GANAGACTTA
              781 CTCTTGATTG TAACGAGGNN TGTGGAACTT CTGGGNNGCN NGGGGGTGGG AAGCCCTCNA
              841 ATATTGGTGG AATCTNCTAC NATATTGNAN TCANGANCTA AAGAATANNG CTGTTAGCTN
              901 GNNNNANGNC NCANCNATAG CANTANNNNN NGGNNNANNT ANGNNTATAN NNNTANNANN
            961 NNNNNNNN
            //
            Primer: env_F
            Region: Rev protein 3'
            Sequence file: RDB08121_A3Jtc.seq check
            >08121_08121_B1IhF_env_F_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNCGNNNAG TGAANGGNTC CTTAGCACTT ATCTGGGACG ATCTGCGGAG
               61 CCTGTGCCTC TTCAGCTACC ACCGCTTGAG AGACTTACTC TTGATTGTAA CGAGGATTGT
              121 GGAACTTCTG GGACGCAGGG GGTGGGAAGC CCTCAAATAT TGGTGGAATC TCCTACAATA
              181 TTGGAGTCAG GAGCTAAAGA ATAGTGCTGT TAGCTTGCTC AATGCCACAG CTATAGCAGT
              241 AGCTGAGGGG ACAGATAGGG TTATAGAAGT AGTACAAGAA GCTTGGCACT GGCCGTCGTT
              301 TTACAACGTC GTGATCTGAG CCTGGGAGAT CTCTGGCTAA CTAGGGAACC CACTGCTTAA
              361 GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC
              421 TGGTAACTAG AGATCAGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC
              481 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT
              541 TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG
              601 GTATTTCACA CCGCATACGT CAAAGCAACC ATAGTACGCG CCCTGTAGCG GCGCATTNAG
              661 CGNGGNGGGT GTGGNGGNTA CGCNNAGCGN GNNCGCTACN CNNNNNNNCN NCNNANCNNC
            721 NNNNNNTTNN NNNTNNNNNC NNNTNNNNTN NNNNNNNNN
            //
            Primer: ColE1ori_F
            Region: lac pro,lacO
            Sequence file: RDB08121_A3Jtd.seq check
            >08121_08121_B1IhF_ColE1ori_F_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN CNCNNCCTCT GNNTTGAGCG TCGATTTTTG TGATGCTCGT
               61 CAGGGGGGCG GANCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
              121 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
              181 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
              241 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
              301 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC
              361 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC
              421 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT
              481 ATGACATGAT TACGAATTCG ATGTACGGGC CAGATATACG CGTATCTGAN GGGACTAGGG
              541 TGTGTTTAGG CGAAAAGCGG GGCTTCNGTT GTACGCGGTT AGGAGTCCCC TCAGGATATA
              601 GTAGTTTCGC TTTTGCATAG GGAGGGGGAA ATGNANTCTT ATGCNATACT CNTGTAGTCN
              661 TGCNACATGG TAACGATGAN TTAGCNACNT GCCNTACNNN NAGANAAAAA NCNNNNNNNN
            721 NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Yanagihashi, Y., Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis. Proc. Natl. Acad. Sci. U.S.A. 114 (33): 8800-8805 (2017). PMID 28768810.
            user_report Ujino, S., Hepatitis C virus utilizes VLDLR as a novel entry pathway. Proc. Natl. Acad. Sci. U.S.A. 113 (1): 188-193 (2016). PMID 26699506.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl