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CSII-CMV-RfA-IRES2-hKO1 (#RDB08070)

Backbone vector plasmid of lentivirus construct driven by CMV promoter by Gateway(R) cloning with hKO1 fluorescent protein marker.

Clone info. Backbone vector plasmid of lentivirus construct driven by CMV promoter by Gateway(R) cloning with hKO1 fluorescent protein marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-CMV-RfA-IRES2-hKO1 ()
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB08070z.seq
Remarks, protocol and/or map (pdf) RDB08070.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08070 CSII-CMV-RfA-IRES2-hKO1 Please contact us. JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-RfA-IRES2-hKO1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08070).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12148_A6Cfp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R
Sequence file: RDB12148_A6Cfa.seq check
>08070_12148_A6Cf_Cm-M_R_C09_09.ab1
    1 GCTGAACTAC CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAATTCCGG
   61 ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT
  121 TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA
  181 TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC
  241 GGTGGTATAT CCAGTGATTT TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA
  301 CGGATCCTAA CTCAAAATCC ACACATTATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG
  361 GGTGCCTAAT GCGGCCGCCA TAGTGACTGG ATATGTTGTG TTTTACAGTA TTATGTAGTC
  421 TGTTTTTTAT GCAAAATCTA ATTTAATATA TTGATATTTA TATCATTTTA CGTTTCTCGT
  481 TCAGCTTTTT TGTACAAACT TGTGATGCTA GCGGATCTGA CGGTTCACTA AACCAGCTCT
  541 GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT
  601 GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC
  661 AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA
  721 CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT
  781 AGGAAAGTCC CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT
  841 GACGTCAATA GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA GTGGGCAGTT
  901 TACCGTAAAT ACTCCACCCA TTGACGTCAA TGGAAAAGTC CCTATTGGCG TTACTATGGG
  961 AACATACGTC ATTATTGACG TCATGGGCGG GGGTCGTTGG GCGGTCAGCC AGGCGGGCCA
 1021 TTTACCGTAA GTTATGTAAC GCGGACTCAT ATATGGCTAT GACTATGACC CCGTATGGAT
1081 ACTATATTCG ATAATCAGTG ATCTGCTGTC CCTGTATAAC CGAAATTTGG ATTTTTT
//
Primer: IRES-R
Sequence file: RDB12148_A6Cfb.seq check
>08070_12148_A6Cf_IRES-R_G09_21.ab1
    1 GCCCAAGGGG GCTCGGCCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG
   61 CCGCTGCAGA ATTCTCGAGA CCGGTAGCAT CACCACTTTG TACAAGAAAG CTGAACGAGA
  121 AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC
  181 ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGTCG ACCTGCAGAC TGGCTGTGTA
  241 TAAGGGAGCC TGACATTTAT ATTCCCCAGA ACATCAGGTT AATGGCGTTT TTGATGTCAT
  301 TTTCGCGGTG GCTGAGATCA GCCACTTCTT CCCCGATAAC GGAGACCGGC ACACTGGCCA
  361 TATCGGTGGT CATCATGCGC CAGCTTTCAT CCCCGATATG CACCACCGGG TAAAGTTCAC
  421 GGGAGACTTT ATCTGACAGC AGACGTGCAC TGGCCAGGGG GATCACCATC CGTCGCCCGG
  481 GCGTGTCAAT AATATCACTC TGTACATCCA CAAACAGACG ATAACGGCTC TCTCTTTTAT
  541 AGGTGTAAAC CTTAAACTGC ATTTCACCAG CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC
  601 ATTTCAATAA ACCGGGCGAC CTCAGCCATC CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG
  661 GCACGCAGAC GACGGGCTTC ATTCTGCATG GTTGTGCTTA CCAGACCGGA GATATTGACA
  721 TCATATATGC CTTGAGCAAC TGATAGCTGT CGCTGTCAAC TGTCACTGTA ATACGCTGCT
  781 TCATAGCATA CCTCTTTTTT GACATACTTC GGGTATACAT ATCAGTATAT ATTCTTATAC
  841 CGCAAAAATC AGCGCGCAAA TACGCATACT GTTATCTGGC TTTTAGTAAG CCCGGATCCA
  901 CGCGTTTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAAGCATTTC
  961 TGCCGACATG GAAAGCCCAT CCACAAACGG CCATGAATGA AACCTTGAAT TCGCCCAGGC
 1021 GGGCAATCAG CACCCTTTGT CGCCTTTGCG TATAATTATT TTGCCCCATG TGAAAACGGG
1081 GGCCGAGAAG TTGTCATATT TGGGCTCACG TTTAAATTCC AAAACCTTGG T
//
Primer: IRES-F
Sequence file: RDB12148_A6Cfc.seq check
>08070_12148_A6Cf_IRES-F_F02_17.ab1
    1 CTCTAATGCC TGTTGCATGG TGTTTAGTCG AGGTTAAAAA CGTCTAAGGC CCCCCGGAAC
   61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGGATGATAA TATGGCCACG GGGATCCCCA
  121 CCATGGTGAG CGTGATCAAG CCCGAGATGA AGATGAAGTA CTTCATGGAC GGCAGCGTGA
  181 ACGGCCACGA GTTCACCGTG GAGGGCGAGG GCACCGGCAA GCCCTACGAG GGCCACCAGG
  241 AGATGACCCT GAGGGTGACA ATGGCCAAGG GCGGCCCCAT GCCCTTCAGC TTCGACCTGG
  301 TGAGCCACAC CTTCTGCTAC GGCCACAGGC CCTTCACCAA GTACCCCGAG GAGATCCCCG
  361 ACTACTTCAA GCAGGCCTTC CCCGAGGGCC TGAGCTGGGA GAGGAGCCTC CAGTTCAAGG
  421 ACGGCGGCTT CGCCGCCGTG AGCGCCCACA TCAGCCTGAG GGGCAACTGC TTCGAGCACA
  481 AGAGCAAGTT CGTGGGCGTG AACTTCCCCG CCGACGGCCC CGTGATGCAG AACCAGAGCA
  541 GCGACTGGGA GCCCAGCACC GAGAAGATCA CCACCTGCGA CGGCGTGCTG AAGGGCGACG
  601 TGACCATGTA CCTGAAGCTG GCCGGCGGCG GCAACCACAA GTGCCAGTTC AAGACCACCT
  661 ACAAGGCCGC CAAAGAAGAT CCTGAAGATG CCCCAGAGCC ACTTCATCGG CCACAGGCTG
  721 GTGAGGAAGA CCGAGGGCAA ACATCACCGA GCTGGTGGAG GACGCCGTGG CCCACTGCTG
  781 ACTGCAGGCT AGAGTTAACA TCGAGGGATC AAGCTTATCG ATAATCAACC CTCTGGATTA
  841 CAAAATTTGT GAAAGATTTG ACTGGTAATT TCTTAACTAT GTTGCTCCTT TTACGCTATG
  901 TGGATACGCT GCTTTTAATG CCTTTGTATC ATGCTATTTG CTTCCCGTTA TGGGCTTTTC
  961 ATTTTCTTCC TCCTTGTATA AATCCTGGGT TGCTGTCTCT TATGAAGGAG TTGTGGGCCC
 1021 GTTGTCAGGC AACGTTGGCG GTGGTTGTGC ACTTGTGTTT GCTGACGCAA CCCCCAACTG
 1081 GATTGGGGGC ATTTGGCCAA CGACCTTGTT CAGCTCCTTT TCCGGGGACT TTTCGCCTTT
 1141 TACCCCCATT CCTTATTTGC CCCAACGGCG AACTTCTATT CGCGTCGCCC TGTGACGTTG
1201 CCGCATGCTT GTGACATCAA GGGGTGTTCT CTGA
//
Primer: HIVgp_R1
Sequence file: RDB12148_A6Cfd.seq check
>08070_12148_A6Cf_HIVgp_R1_A10_01.ab1
    1 CGGGCAATCC TAATTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG
   61 GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC
  121 CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGGCC GCTTGCTGTG
  181 CGGTGGTCTT ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT
  241 TTATCTCTAT CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA
  301 GTTCTTCTGA TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT
  361 CTGATGTTTC TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA
  421 CTATATGTTT TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC
  481 GCGATCTAAT TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC
  541 CTCCGCTAGT CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC
  601 GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA
  661 AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA
  721 TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA
  781 GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT
  841 CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAACGCGCT GCTTATATAG ACCTCCCACC
  901 GTACACGCCT ACCGCCCATT TGCGTCAATG GGGGCGGGAG TTGTTACGAC ATTTTTGGAA
  961 AGTCCCGTTT GATTTTGGGT GCCAAAACAA ACTCCCATTT GAACGTCAAT GGGGTGGAGA
 1021 CTTGGAAATT CCCGTGAGTC AAACCGCTAT CCACGCCAAT GGATGGTACT GCCAAACGGC
1081 AATCATCAAT GTAATAGCGA TGGAACTAAT TACGGTTAGA AA
//
Primer: BGH_rev2
Sequence file: RDB12148_A6Cfe.seq check
>08070_12148_A6Cf_BGH_rev2_B10_04.ab1
    1 CCCGGGCCGT TAGTAAGCGC GGGGGAGGGG CAGCAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
  301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
  361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
  421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
  481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
  541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
  601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
  661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
  721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT
  781 CGACGGTATC GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG
  841 CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA
  901 TTGAGGGCCG AAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAAATCCAG GTGGCAACAC
  961 AGGCGAGCAG CCCATGGGAA GGACGTCAGC TTTCCCTCGA CACACCACGG GAATTGTCAG
 1021 TGCCTCAACA GCCCGAGCCC CTGTCCAGCA GCGGGCAGCA GCGGCGATGA GTTCGGTCGT
1081 GACCATTAGG ATGGGGGAAA GCTGTAAAGA TC
//
Primer: SV40pro_F
Sequence file: RDB12148_A6Cff.seq check
>08070_12148_A6Cf_SV40pro_F_C10_07.ab1
    1 CCCTGGTTGG TGGAACAAAT TCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
   61 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA
  121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA
  181 TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG
  241 AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG
  301 TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA
  361 ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG
  421 TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC
  481 CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG
  541 AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT
  601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA
  661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAAATAA
  721 AGCAATAGCA TCACAAATTT CACAAATAAA AGCATTTTTT TCACTGCATT CTAGTTGTGG
  781 TTTGTCCAAA ACCTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG
  841 AGCTTGGGCG TAATCATGGT CAATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA
  901 ATTCCACACA AACATACAAG CCGGAAAGCA ATAAAAGTGT TAAAAGCCTG GGGGTGCCCT
  961 AATGAGTGAG CTAAACTCAC ATTAAATTGC GTTTGCGCTT CACTGCCCCG CTTTCCAGTC
 1021 GGGAAAACCC TGGTTCGTGC CAAGCTTGCC ATTAATGGAA TTCGGGCCCA AACGGCGCGG
 1081 GGGAAGAGGC GGATTATGCC GTAATGGGGG GCCTTCTTCC GGCATTTCCT CCGCCTTCCA
1141 CATTAAACT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Iwasawa, C., Increased Cytotoxicity of Herpes Simplex Virus Thymidine Kinase Expression in Human Induced Pluripotent Stem Cells. Int. J. Mol. Sci. 20 (4). pii: E810 (2019). PMID 30769780. [link to Journal]