Resource data sheet
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pCtFLATAki-Pspac323.08-SalI-spac323.08(+)-kanMX6T (#RDB08050)

Knock-in clone for S. pombe SPAC323.08

Clone info. Knock-in clone for S. pombe SPAC323.08, ribonuclease MRP complex subunit (predicted).
Comment FLATA = FLAG-TEVcs-protein A.
Vector backbone pCtFLATAki-kanMX6 (Plasmid)
Selectable markers Amp^r (E. coli), Kan^r (S. pombe)
Gene/insert name S. pombe SPAC323.08 genomic DNA
Reference of insert sequence AB623237 (DDBJ accession number)
Depositor|Developer Isobe, Toshiaki | Takeda, Jun |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Saito, Y. et al., PLoS One 9 (11): e112488, 2014).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Saito, Y. et al., PLoS One 9 (11): e112488, 2014) 。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB08050 pCtFLATAki-Pspac323.08-SalI-spac323.08(+)-kanMX6T Under QC test. Please contact us. DNA solution Please contact us.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCtFLATAki-Pspac323.08-SalI-spac323.08(+)-kanMX6T was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08050).

Reference section:

Saito, Y., Takeda, J., Adachi, K., Nobe, Y., Kobayashi, J., Hirota, K., Oliveira, D.V.,
Taoka, M., Isobe, T., RNase MRP cleaves pre-tRNASer-Met in the tRNA maturation pathway. Saito, Y. et al., PLoS One 9 (11): e112488 (2014). PMID 25401760. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Saito, Y., RNase MRP cleaves pre-tRNASer-Met in the tRNA maturation pathway. Saito, Y. et al., PLoS One 9 (11): e112488 (2014). PMID 25401760. [link to RRC of NBRP]