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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pRBS-EGFP

Rbpj-binding sequence containing reporter GFP plasmid

Catalog number RDB08039
Resource name pRBS-EGFP
Clone info. The RBS (Rbpj-binding sequences, hexamerized 50 bp EBNA2 response element of the TP-1 promoter) in the original plasmid pGA981-6 was inserted into the EGFP expression plasmid in pEGFP-N1(clontech).
Vector backbone pEGFP-N1 (Plasmid)
Selectable markers Kan^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Taketo, Makoto Mark | Sonoshita, Masahiro |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The RECIPIENT must obtain a prior written consent from Prof. Makoto Taketo of the Kyoto University and Prof. Tasuku Honjo of the Kyoto University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested.(Cancer Cell, 19, 125-137(2011).). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The Use of the BIOLOGICAL RESOURCE is limited to the RECIPIENT and his/her directly supervised personnel. The use of the BIOLOGICAL RESOURCE is limited only to academic research. The RECIPIENT understands that the plasmid contains a commercial vector pEGFP-N1 by Clontech that contains the patented sequence for EGFP. The RECIPIENT agrees that DEPOSITOR takes no legal responsibility for the use of the BIOLOGICAL RESOURCE, and is exempt from any legal issues that may be caused by using the BIOLOGICAL RESOURCE.
            Remarks ((Additional form)) Approval Form(FormD8039)
            Obtain written concent with FormD8039.
            Please use Schedule A (GFP-A) additionally.
            Please use FormD8039 to obtain approval of Profs. Makoto Taketo and Tasuku Honjo.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、事前に京都大学武藤誠教授ならびに京都大学本庶佑教授から提供承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cancer Cell, 19, 125-137(2011).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、利用者とその直接的指導下に有る者に限る。本件リソースの利用は、学術目的に限る。利用者は、本件リソースが特許配列であるEGFPのpEGFP-N1(クロンテック社製)ベクターを含むことを了承する。利用者は本件リソースの使用によって発生した利用者の損害について、寄託者の法的責任を問わないことを了承する。
            備考 【追加提供依頼書】専用承諾書(FormD8039)
            専用承諾書(FormD8039)により承諾を得ること
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。
            武藤誠教授ならびに本庶佑教授の提供承諾にはFormD8039をお使いください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08039 pRBS-EGFP DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Sonoshita, M., Suppression of colon cancer metastasis by Aes through inhibition of Notch signaling. Cancer Cell, 19, 125-137 (2011). PMID 21251616.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content GFP Resources (English text)
            Featured content Notch signaling pathway (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15877_A8Ckp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EGFP-N
            Sequence file: RDB15877_A8Cka.seq check
            >08039_15877_A8Ck_EGFP-N_C02_08_ABI24.ab1
                1 GACGGGGGGA CGCTCTCGCC CTTGCTCACC ATGGTGGCGA CCGGTGGATC CCGGGCCCGC
               61 GGTACCGTCG ACTGCAGAAT TCGAAGCTTG AGCTCGATCG AGGGATTGCA AGTAAACACA
              121 GTTGTGTCAA AAGCAAGTGT AAGCAGAAGC TGAAGTGATC TGCCTTTTAT GCCCAAGATG
              181 CATACAGTGG ATCTACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC ACGAGTCGGG
              241 ATCTACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC ACGAGTCGGG ATCTACTATT
              301 TTCCCACGGT GCCCTTCCGC CCATTTTCCC ATGAGTCGGG ATCTACTATT TTCCCACGGT
              361 GCCCTTCCGC CCATTTTCCC ACGAGTCGGG ATCTACTATT TTCCCACGGT GCCCTTCCGC
              421 CCATTTTCCC ACGAGTCGGG ATCTACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC
              481 ATGAGTCGGG ATCCTCTAGA GTCGACCTGC AGGCATGCAA GCTCATGTGA GCAAAAGGCC
              541 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
              601 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
              661 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
              721 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
              781 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
              841 ACGAACCCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
              901 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
              961 GCGAGGTATG TAGGCGGGTG CTACAGAGTT CTTGAAGTGG TGGCCTACTA CGGCTACACT
             1021 AGAAGGACAG TATTTGGGTA TCTGCGCTCT GCCTGGAGGC AGCTACTTTC GGAAGAGAGT
            1081 TGGTAGCTCA TGAATCGGGC CAACCAAACC CACCGCTGGG TTAGTG
            //
            Primer: pAxEF R2
            Sequence file: RDB15877_A8Ckb.seq check
            >08039_15877_A8Ck_pAxEFR2_D02_11_ABI24.ab1
                1 CAGGGGAGAA TGCTTTATTT GTGAAGTTGT GATGCTATTG CTTTATTTGT AACCAGTTAT
               61 AAGCTGCAAG TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
              121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
              181 ATGATCTAGA GTCGCGGCCG CTTTACTTGT ACAGCTCGTC CATGCCGAGA GTGATCCCGG
              241 CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGCTCA
              301 GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG TCGCCGATGG
              361 GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCGATG TTGTGGCGGA
              421 TCTTGAAGTT CACCTTGATG CCGTTCTTCT GCTTGTCGGC CATGATATAG ACGTTGTGGC
              481 TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC
              541 CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT
              601 TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG GACGTAGCCT TCGGGCATGG
              661 CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC
              721 CGTAGGTCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA
              781 TGAACTTCAG GGTCAGCTTG CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA
              841 ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA
              901 GCTCCTCGCC CTTGCTCACC ATGGTGGCGA CCGGTGGATC CCGGGCCCGC GGTACCGTCG
              961 ACTGCAGAAA TTCGAAGCTT TGAGCTCGAT CGAGGGATTG CAAGTAAACA CAGTTGTGTC
             1021 AAAAGCAAGG TGTAGCAGAG CTGAGTGATC TGCCCTTTTA TGGCCCAAGA TGCATACAGT
            1081 GATCTACCTA TTATCCCCAC CGATGCCCGT TCCGACCCCA ATTTTTCGC
            //
            Primer: f1ori_R3
            Sequence file: RDB15877_A8Ckc.seq check
            >08039_15877_A8Ck_f1ori_R3_E02_14_ABI24.ab1
                1 GGCGGGTCAT TTCGGGGGAT GTGCGCGGAC CCCTATTTGT TTATTTTTCT AAATACATTC
               61 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG
              121 GAAGAGTCCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG
              181 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC
              241 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT
              301 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT
              361 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC
              421 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
              481 TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT
              541 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA
              601 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT
              661 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG CAGCGCGGCT
              721 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC
              781 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT
              841 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA
              901 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG
              961 GGATGGAAGC CCGGTCTTGT CGATCAGGAT GATCTGGACG AGAGCATCAG GGGCTCGCGC
             1021 CAGCCCGAAC TGATCGCCAG CTCAAGGCGG AGCCATGGCT CGGACCGGCG AGATCTCGTT
             1081 CGTGAACCAT TGACCGATGG GTTGCTTGCG ATATTCATGT GAATTGGCTG CACTATATCG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Sonoshita, M., Suppression of colon cancer metastasis by Aes through inhibition of Notch signaling. Cancer Cell, 19, 125-137 (2011). PMID 21251616.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl