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pRBS-EGFP

Rbpj-binding sequence containing reporter GFP plasmid

Catalog number RDB08039
Resource name pRBS-EGFP
Clone info. The RBS (Rbpj-binding sequences, hexamerized 50 bp EBNA2 response element of the TP-1 promoter) in the original plasmid pGA981-6 was inserted into the EGFP expression plasmid in pEGFP-N1(clontech).
Vector backbone pEGFP-N1 (Plasmid)
Selectable markers Kan^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Taketo, Makoto Mark | Sonoshita, Masahiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT must obtain a prior written consent from Prof. Makoto Taketo of the Kyoto University and Prof. Tasuku Honjo of the Kyoto University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested.(Cancer Cell, 19, 125-137(2011).). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The Use of the BIOLOGICAL RESOURCE is limited to the RECIPIENT and his/her directly supervised personnel. The use of the BIOLOGICAL RESOURCE is limited only to academic research. The RECIPIENT understands that the plasmid contains a commercial vector pEGFP-N1 by Clontech that contains the patented sequence for EGFP. The RECIPIENT agrees that DEPOSITOR takes no legal responsibility for the use of the BIOLOGICAL RESOURCE, and is exempt from any legal issues that may be caused by using the BIOLOGICAL RESOURCE.
Remarks ((Additional form)) GFP-A, Approval Form(FormD8039)
Obtain written concent with FormD8039.
Please use Schedule A (GFP-A) additionally.
Please use FormD8039 to obtain approval of Profs. Makoto Taketo and Tasuku Honjo.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08039 pRBS-EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Fluorescent reporter clone of Notch signal activation (English text)
Featured content GFP Resources (English text)
Featured content Notch signaling pathway (English text)

References

original Sonoshita, M., Suppression of colon cancer metastasis by Aes through inhibition of Notch signaling. Cancer Cell, 19, 125-137 (2011). PMID 21251616.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15877_A8Ckp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N RDB15877_A8Cka.seq
>08039_15877_A8Ck_EGFP-N_C02_08_ABI24.ab1
    1 GACGGGGGGA CGCTCTCGCC CTTGCTCACC ATGGTGGCGA CCGGTGGATC CCGGGCCCGC
   61 GGTACCGTCG ACTGCAGAAT TCGAAGCTTG AGCTCGATCG AGGGATTGCA AGTAAACACA
  121 GTTGTGTCAA AAGCAAGTGT AAGCAGAAGC TGAAGTGATC TGCCTTTTAT GCCCAAGATG
  181 CATACAGTGG ATCTACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC ACGAGTCGGG
  241 ATCTACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC ACGAGTCGGG ATCTACTATT
  301 TTCCCACGGT GCCCTTCCGC CCATTTTCCC ATGAGTCGGG ATCTACTATT TTCCCACGGT
  361 GCCCTTCCGC CCATTTTCCC ACGAGTCGGG ATCTACTATT TTCCCACGGT GCCCTTCCGC
  421 CCATTTTCCC ACGAGTCGGG ATCTACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC
  481 ATGAGTCGGG ATCCTCTAGA GTCGACCTGC AGGCATGCAA GCTCATGTGA GCAAAAGGCC
  541 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
  601 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
  661 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
  721 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
  781 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
  841 ACGAACCCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
  901 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
  961 GCGAGGTATG TAGGCGGGTG CTACAGAGTT CTTGAAGTGG TGGCCTACTA CGGCTACACT
 1021 AGAAGGACAG TATTTGGGTA TCTGCGCTCT GCCTGGAGGC AGCTACTTTC GGAAGAGAGT
1081 TGGTAGCTCA TGAATCGGGC CAACCAAACC CACCGCTGGG TTAGTG
//
Primer: pAxEF R2 RDB15877_A8Ckb.seq
>08039_15877_A8Ck_pAxEFR2_D02_11_ABI24.ab1
    1 CAGGGGAGAA TGCTTTATTT GTGAAGTTGT GATGCTATTG CTTTATTTGT AACCAGTTAT
   61 AAGCTGCAAG TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
  121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
  181 ATGATCTAGA GTCGCGGCCG CTTTACTTGT ACAGCTCGTC CATGCCGAGA GTGATCCCGG
  241 CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGCTCA
  301 GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG TCGCCGATGG
  361 GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCGATG TTGTGGCGGA
  421 TCTTGAAGTT CACCTTGATG CCGTTCTTCT GCTTGTCGGC CATGATATAG ACGTTGTGGC
  481 TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC
  541 CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT
  601 TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG GACGTAGCCT TCGGGCATGG
  661 CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC
  721 CGTAGGTCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA
  781 TGAACTTCAG GGTCAGCTTG CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA
  841 ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA
  901 GCTCCTCGCC CTTGCTCACC ATGGTGGCGA CCGGTGGATC CCGGGCCCGC GGTACCGTCG
  961 ACTGCAGAAA TTCGAAGCTT TGAGCTCGAT CGAGGGATTG CAAGTAAACA CAGTTGTGTC
 1021 AAAAGCAAGG TGTAGCAGAG CTGAGTGATC TGCCCTTTTA TGGCCCAAGA TGCATACAGT
1081 GATCTACCTA TTATCCCCAC CGATGCCCGT TCCGACCCCA ATTTTTCGC
//
Primer: f1ori_R3 RDB15877_A8Ckc.seq
>08039_15877_A8Ck_f1ori_R3_E02_14_ABI24.ab1
    1 GGCGGGTCAT TTCGGGGGAT GTGCGCGGAC CCCTATTTGT TTATTTTTCT AAATACATTC
   61 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG
  121 GAAGAGTCCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG
  181 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC
  241 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT
  301 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT
  361 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC
  421 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
  481 TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT
  541 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA
  601 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT
  661 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG CAGCGCGGCT
  721 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC
  781 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT
  841 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA
  901 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG
  961 GGATGGAAGC CCGGTCTTGT CGATCAGGAT GATCTGGACG AGAGCATCAG GGGCTCGCGC
 1021 CAGCCCGAAC TGATCGCCAG CTCAAGGCGG AGCCATGGCT CGGACCGGCG AGATCTCGTT
 1081 CGTGAACCAT TGACCGATGG GTTGCTTGCG ATATTCATGT GAATTGGCTG CACTATATCG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sonoshita, M., Suppression of colon cancer metastasis by Aes through inhibition of Notch signaling. Cancer Cell, 19, 125-137 (2011). PMID 21251616.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl