RDB08039 - pRBS-EGFP

Catalog number	RDB08039
Resource name	pRBS-EGFP
Clone info.	The RBS (Rbpj-binding sequences, hexamerized 50 bp EBNA2 response element of the TP-1 promoter) in the original plasmid pGA981-6 was inserted into the EGFP expression plasmid in pEGFP-N1(clontech).
Vector backbone	pEGFP-N1 (Plasmid)
Selectable markers	Kan^r
Gene/insert name	Aequorea victoria GFP cDNA
Depositor	Taketo, Makoto Mark \| Sonoshita, Masahiro \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The RECIPIENT must obtain a prior written consent from Prof. Makoto Taketo of the Kyoto University and Prof. Tasuku Honjo of the Kyoto University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested.(Cancer Cell, 19, 125-137(2011).). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The Use of the BIOLOGICAL RESOURCE is limited to the RECIPIENT and his/her directly supervised personnel. The use of the BIOLOGICAL RESOURCE is limited only to academic research. The RECIPIENT understands that the plasmid contains a commercial vector pEGFP-N1 by Clontech that contains the patented sequence for EGFP. The RECIPIENT agrees that DEPOSITOR takes no legal responsibility for the use of the BIOLOGICAL RESOURCE, and is exempt from any legal issues that may be caused by using the BIOLOGICAL RESOURCE.
Remarks	((Additional form)) GFP-A, Approval Form(FormD8039) Obtain written concent with FormD8039. Please use Schedule A (GFP-A) additionally. Please use FormD8039 to obtain approval of Profs. Makoto Taketo and Tasuku Honjo.

Terms and conditions set forth by the DEPOSITOR

The RECIPIENT must obtain a prior written consent from Prof. Makoto Taketo of the Kyoto University and Prof. Tasuku Honjo of the Kyoto University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested.(Cancer Cell, 19, 125-137(2011).). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The Use of the BIOLOGICAL RESOURCE is limited to the RECIPIENT and his/her directly supervised personnel. The use of the BIOLOGICAL RESOURCE is limited only to academic research. The RECIPIENT understands that the plasmid contains a commercial vector pEGFP-N1 by Clontech that contains the patented sequence for EGFP. The RECIPIENT agrees that DEPOSITOR takes no legal responsibility for the use of the BIOLOGICAL RESOURCE, and is exempt from any legal issues that may be caused by using the BIOLOGICAL RESOURCE.

Remarks

((Additional form)) GFP-A, Approval Form(FormD8039)
Obtain written concent with FormD8039.
Please use Schedule A (GFP-A) additionally.
Please use FormD8039 to obtain approval of Profs. Makoto Taketo and Tasuku Honjo.

Distribution information (domestic users)

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提供条件	利用者は、事前に京都大学武藤誠教授ならびに京都大学本庶佑教授から提供承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cancer Cell, 19, 125-137(2011).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、利用者とその直接的指導下に有る者に限る。本件リソースの利用は、学術目的に限る。利用者は、本件リソースが特許配列であるEGFPのpEGFP-N1(クロンテック社製)ベクターを含むことを了承する。利用者は本件リソースの使用によって発生した利用者の損害について、寄託者の法的責任を問わないことを了承する。
備考	【追加提供依頼書】GFP-A, 専用承諾書(FormD8039) 専用承諾書(FormD8039)により承諾を得ること Schedule A（GFP-A, 英語版のみ）が追加で必要です。武藤誠教授ならびに本庶佑教授の提供承諾にはFormD8039をお使いください。

提供条件

利用者は、事前に京都大学武藤誠教授ならびに京都大学本庶佑教授から提供承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cancer Cell, 19, 125-137(2011).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、利用者とその直接的指導下に有る者に限る。本件リソースの利用は、学術目的に限る。利用者は、本件リソースが特許配列であるEGFPのpEGFP-N1(クロンテック社製)ベクターを含むことを了承する。利用者は本件リソースの使用によって発生した利用者の損害について、寄託者の法的責任を問わないことを了承する。

備考

【追加提供依頼書】GFP-A, 専用承諾書(FormD8039)
専用承諾書(FormD8039)により承諾を得ること
Schedule A（GFP-A, 英語版のみ）が追加で必要です。
武藤誠教授ならびに本庶佑教授の提供承諾にはFormD8039をお使いください。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08039	pRBS-EGFP	DNA solution

Featured content	Fluorescent reporter clone of Notch signal activation (English text)
Featured content	GFP Resources (English text)
Featured content	Notch signaling pathway (English text)

original	Sonoshita, M., Suppression of colon cancer metastasis by Aes through inhibition of Notch signaling. Cancer Cell, 19, 125-137 (2011). PMID 21251616.

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Primer: EGFP-N	RDB15877_A8Cka.seq
>08039_15877_A8Ck_EGFP-N_C02_08_ABI24.ab1 1 GACGGGGGGA CGCTCTCGCC CTTGCTCACC ATGGTGGCGA CCGGTGGATC CCGGGCCCGC 61 GGTACCGTCG ACTGCAGAAT TCGAAGCTTG AGCTCGATCG AGGGATTGCA AGTAAACACA 121 GTTGTGTCAA AAGCAAGTGT AAGCAGAAGC TGAAGTGATC TGCCTTTTAT GCCCAAGATG 181 CATACAGTGG ATCTACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC ACGAGTCGGG 241 ATCTACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC ACGAGTCGGG ATCTACTATT 301 TTCCCACGGT GCCCTTCCGC CCATTTTCCC ATGAGTCGGG ATCTACTATT TTCCCACGGT 361 GCCCTTCCGC CCATTTTCCC ACGAGTCGGG ATCTACTATT TTCCCACGGT GCCCTTCCGC 421 CCATTTTCCC ACGAGTCGGG ATCTACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC 481 ATGAGTCGGG ATCCTCTAGA GTCGACCTGC AGGCATGCAA GCTCATGTGA GCAAAAGGCC 541 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 601 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 661 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 721 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 781 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 841 ACGAACCCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 901 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 961 GCGAGGTATG TAGGCGGGTG CTACAGAGTT CTTGAAGTGG TGGCCTACTA CGGCTACACT 1021 AGAAGGACAG TATTTGGGTA TCTGCGCTCT GCCTGGAGGC AGCTACTTTC GGAAGAGAGT 1081 TGGTAGCTCA TGAATCGGGC CAACCAAACC CACCGCTGGG TTAGTG //
Primer: pAxEF R2	RDB15877_A8Ckb.seq
>08039_15877_A8Ck_pAxEFR2_D02_11_ABI24.ab1 1 CAGGGGAGAA TGCTTTATTT GTGAAGTTGT GATGCTATTG CTTTATTTGT AACCAGTTAT 61 AAGCTGCAAG TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG 121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT 181 ATGATCTAGA GTCGCGGCCG CTTTACTTGT ACAGCTCGTC CATGCCGAGA GTGATCCCGG 241 CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGCTCA 301 GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG TCGCCGATGG 361 GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCGATG TTGTGGCGGA 421 TCTTGAAGTT CACCTTGATG CCGTTCTTCT GCTTGTCGGC CATGATATAG ACGTTGTGGC 481 TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC 541 CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT 601 TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG GACGTAGCCT TCGGGCATGG 661 CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC 721 CGTAGGTCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA 781 TGAACTTCAG GGTCAGCTTG CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA 841 ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA 901 GCTCCTCGCC CTTGCTCACC ATGGTGGCGA CCGGTGGATC CCGGGCCCGC GGTACCGTCG 961 ACTGCAGAAA TTCGAAGCTT TGAGCTCGAT CGAGGGATTG CAAGTAAACA CAGTTGTGTC 1021 AAAAGCAAGG TGTAGCAGAG CTGAGTGATC TGCCCTTTTA TGGCCCAAGA TGCATACAGT 1081 GATCTACCTA TTATCCCCAC CGATGCCCGT TCCGACCCCA ATTTTTCGC //
Primer: f1ori_R3	RDB15877_A8Ckc.seq
>08039_15877_A8Ck_f1ori_R3_E02_14_ABI24.ab1 1 GGCGGGTCAT TTCGGGGGAT GTGCGCGGAC CCCTATTTGT TTATTTTTCT AAATACATTC 61 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG 121 GAAGAGTCCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 181 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 241 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 301 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 361 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 421 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 481 TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT 541 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA 601 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT 661 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG CAGCGCGGCT 721 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC 781 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT 841 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA 901 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG 961 GGATGGAAGC CCGGTCTTGT CGATCAGGAT GATCTGGACG AGAGCATCAG GGGCTCGCGC 1021 CAGCCCGAAC TGATCGCCAG CTCAAGGCGG AGCCATGGCT CGGACCGGCG AGATCTCGTT 1081 CGTGAACCAT TGACCGATGG GTTGCTTGCG ATATTCATGT GAATTGGCTG CACTATATCG //