Resource data sheet
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CS-RfA-ETV

Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Venus marker driven by EF-1 alpha promoter.

Catalog number RDB08020
Resource name CS-RfA-ETV
Clone info. Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Venus marker driven by EF-1 alpha promoter.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-RfA-ETV ()
Selectable markers Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08020 CS-RfA-ETV DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08020z.seq from Depositor
Electronic file PDF RDB08020.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12130_A5Ecp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R RDB12130_A5Eca.seq
>08020_12130_A5Ec_Cm_M_R_A12_03.ab1
    1 GCTTTGAATA CCTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG
   61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
  121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
  181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
  241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
  301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
  361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
  421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG
  481 TTCAGCTTTT TTGTACAAAC TTGTGATAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA
  541 AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT
  601 TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT
  661 AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC
  721 TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG
  781 GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA
  841 GTACAGCAAA AACTATTCTT AAACCTACCA AGCCTCCTAC TATCATTATG AATAAATTTT
  901 ATATACCACA GCCCAATTTG TTATGTTAAA CCCAATTTCC ACAAACTTTG CCCATTTATC
  961 TAATTCCCAT AATTCTTGTT CATTCTTTTC TGCTTGCTTT GCGATCTTCA TACGAGTGTA
 1021 TAAGCTTGTG TATTGTTAGT TTCTCTGTGC CACTCATCCA GGTCCGTGTG AATCCGAATC
1081 TTGTTTCCGA G
//
Primer: EF1a-N_R RDB12130_A5Ecb.seq
>08020_12130_A5Ec_EF1a-N_R_B12_06.ab1
    1 AACGAAATCA CGGGGGATGT GGGCGATGTG CGCTCTGCCC ACTGACGGGC ACCGGAGCCT
   61 CACGAAGCTT GAATTATCAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT
  121 ATAAATATCA ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA
  181 ACACAACATA TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA
  241 CATTTATATT CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT
  301 GAGATCAGCC ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT
  361 CATGCGCCAG CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC
  421 TGACAGCAGA CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT
  481 ATCACTCTGT ACATCCACAA ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT
  541 AAACTGCATT TCACCAGCCC CTGTTCTCGT CAGCAAAAGA GCCGTTCATT TCAATAAACC
  601 GGGCGACCTC AGCCATCCCT TCCTGATTTT CCGCTTTCCA GCGTTCGGCA CGCAGACGAC
  661 GGGCTTCATT CTGCATGGTT GTGCTTACCA GACCGGAGAT ATTGACATCA TATATGCCTT
  721 GAGCAACTGA TAGCTGTCGC TGTCAACTGT CACTGTAATA CGCTGCTTCA TAGCATACCT
  781 CTTTTTGACA TACTTCGGGT ATACATATCA GTATATATTC TTATACCGCA AAAATCAGCG
  841 CGCAAATACG CATACTGTTA TCTGGCTTTT AGTAAGCCGG ATCCACGCGT TTACGCCCCG
  901 CCCTGCCACT CATCGCAGTA CTGTTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC
  961 ATCACAAACG GGCATGATGA AACCCTGAAT CGCAGCGGCA TCAGCACCTT TGTCGGCTTT
 1021 GCGTATATAT TTTGCCCAAT GCTGAAAACG GGGGCGAAGG AGTGTCGATA TGGTCCACGT
1081 TTAAATCGAA ACTTGGT
//
Primer: pAxEF_F1 RDB12130_A5Ecc.seq
>08020_12130_A5Ec_pAxEF_F1_C12_09.ab1
    1 GTTTTTCTTC ATTTCAGTGT CGTGATTAAT TAACCGGCCG CATAAGGTGG CGGTGGCAGC
   61 TAGACTAGCG CTACCGGTCG CCACCATGTC TAGATTAGAT AAAAGTAAAG TGATTAACAG
  121 CGCATTAGAG CTGCTTAATG AGGTCGGAAT CGAAGGTTTA ACAACCCGTA AACTCGCCCA
  181 GAAGCTAGGT GTAGAGCAGC CTACATTGTA TTGGCATGTA AAAAATAAGC GGGCTTTGCT
  241 CGACGCCTTA GCCATTGAGA TGTTAGATAG GCACCATACT CACTTTTGCC CTTTAGAAGG
  301 GGAAAGCTGG CAAGATTTTT TACGTAATAA CGCTAAAAGT TTTAGATGTG CTTTACTAAG
  361 TCATCGCGAT GGAGCAAAAG TACATTTAGG TACACGGCCT ACAGAAAAAC AGTATGAAAC
  421 TCTCGAAAAT CAATTAGCCT TTTTATGCCA ACAAGGTTTT TCACTAGAGA ATGCATTATA
  481 TGCACTCAGC GCTGTGGGGC ATTTTACTTT AGGTTGCGTA TTGGAAGATC AAGAGCATCA
  541 AGTCGCTAAA GAAGAAAGGG AAACACCTAC TACTGATAGT ATGCCGCCAT TATTACGACA
  601 AGCTATCGAA TTATTTGATC ACCAAGGTGC AGAGCCAGCC TTCTTATTCG GCCTTGAATT
  661 GATCATATGC GGATTAGAAA AACAACTTAA ATGTGAAAGT GGGTCCGCGT ACAGCGGATC
  721 CGGCGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAAAATC CCGGGGCCCA
  781 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
  841 GCGACGTAAA CGGGCCACAA GTTCAGCGTG TCCGCGAGAC GAGACGATGC CACCTACGGC
  901 AAGCTGACCT GAGCTGATCT GCACACGGGC AGGCTGCCCG TGCCTGACCC ACCCTTCGTG
  961 ACCACCTTGG CTTACCGCTT GCAGTGCTCG CCGGCTAACC CGGATCCATG AGCAGCACGG
1021 AACTCTCAAG TCGCATGCTG AGCC
//
Primer: PRE_rev RDB12130_A5Ecd.seq
>08020_12130_A5Ec_PRE_rev_F02_17.ab1
    1 CGGGTAATTA CCATAGCCCG TAAAAGGAGC AACATAGTTA AGAATACCAG TCAATCTTTC
   61 ACAAATTTTG TAATCCAGAG GTTGATTATC GATAAGCTTG ATCCCTCGAG TGCGGCCAAT
  121 TTTTACTTGT ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC
  181 AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GTAGCTCAGG
  241 TAGTGGTTGT CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG
  301 TCGGCGAGCT GCACGCCGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG
  361 CCGTTCTTCT GCTTGTCGGC GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC
  421 TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC
  481 ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG
  541 AAGAAGATGG TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC
  601 TGCTTCATGT GGTCGGGGTA GCGGGCGAAG CACTGCAGGC CGTAGCCCAG GGTGGTCACG
  661 AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TCAGCTTCAG GGTCAGCTTG
  721 CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG
  781 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC
  841 ATGGGCCCGG GATTTTCCTC CACGTCACCG CATGTTAGAA GACTTCCTCT GCCCTCGCCG
  901 GATCCGCTGT ACGCGGACCC ACTTTCACAT TTAAGTTGTT TTTCTAATCC GCATATGATC
  961 AATTCAAGGC CGAATAAGAC GCTGGCTCTG CACCTTGGTG ATCAAATAAT TCGATAGCTT
 1021 GTCGTAATAA TGGCGGCATA CTATCAGTAG TAGGTGTTCC CTTTCTTCTT AGCGACTTGA
 1081 TGCTCTGATC TTCCCATACG CACTTAAAGT AATGCCTCAC AGCGCTGAAT GCATAATAAT
1141 GCAATTCTTC TATGGAACTG GTTGCCATTA AAAAGGCCTA AACTTGAATC
//
Primer: CMV-Forward RDB12130_A5Ece.seq
>08020_12130_A5Ec_CMV-Forward_A10_01.ab1
    1 CGGAAACTCC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
  121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
  181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
  241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
  301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
  361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
  421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
  481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
  541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
  601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
  661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
  721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGCGGCCGCT
  781 GATCTTCAGA CCTGGAGGAG GAGATATGAG GGACAATTGG AGAAGTGAAT TATATAAATA
  841 TAAAGTAGTA AAAATTGAAC CATTAGGAGT AGCACCCACC AAGGCAAAGA GAAGAGTGGT
  901 GCAGAGAGAA AAAAAGAGCA GTGGGAATAG GAGCTTTGTT CCCTTGCGTT TCTTGAGAGG
  961 CAGCAGGAAA GCACTATTGG CCGCCAGCGT CGATGACGCT GACCGTACCA GGCCAGACAT
1021 TATTGTCTGG CAATAGTTGC CAGC
//
Primer: BGH_rev RDB12130_A5Ecf.seq
>08020_12130_A5Ec_BGH_rev_E12_15.ab1
    1 CGACTCGGGC CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT
   61 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG
  121 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA
  181 CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC ATGTGATGAA ATGCTAGGCG
  241 GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA TCCATCCCAT GCAGGCTCAC
  301 AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT TCTACCTTCT CTTGCTCAAC
  361 TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT CTGATCCCTG GCCCTGGTGT
  421 GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG ATCCACAGAT CAAGGATATC
  481 TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT
  541 AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT ACCTGAGGTG TGACTGGAAA
  601 ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA TCAGCATTGG GTAGCTGCTG
  661 TATTGCTACT GTGATGCTCC ATGTTTTTTC TAGGGTCTCG ACGGTATCGA TGCGGGAGCG
  721 CCCAAAGGGA GATTCGACTC GTCTGAGGCG AGCAGACCCG GAAAGCGCAA GCGCACAGCC
  781 CCGGAGGAGT CGCTGATTGA GCGAGACTAC GAGGACGTCC GGCAATCAGT GTCACCAGCC
  841 ACAGCATGAA GACGTCAGCT TCCGAACAAC GCGGAATTGG CATTCAATCT GACCGTGTCA
901 AGCGGCAGCA GGCGCTAATG ATCG
//
Primer: SV40pro_F RDB12130_A5Ecg.seq
>08020_12130_A5Ec_SV40pro_F_F12_18.ab1
    1 CCCGGTCAGT GACATCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
  121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
  181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
  241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
  301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
  361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
  421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
  481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
  541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
  661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
  841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
  901 ACGAGCCGGA AGCATAAAGT GTAAGCCCTG GGGGTGCCTA ATGAGTGAGC TAACTCACAT
  961 TAAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACTGGTCGTG CAGCTGCATT
 1021 AATGGAATTC GCAACGCCGC GGGGAGAGCG GTTTGCCGTA TTGGGGCGCT CTTTCGACGT
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219.
user report Mitsuda, Y., RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci. Rep. 8 (1): 6423 (2018). PMID 29686309.
user report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
user report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
user report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Advances 1: 1440-1451 (2017). PMID 29296785.

2018.10.22

GNP_filter3_RDBDEP_html_181012.pl