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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CS-RfA-ETV

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Venus marker driven by EF-1 alpha promoter.

Catalog number RDB08020
Resource name CS-RfA-ETV
Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Venus marker driven by EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-RfA-ETV ()
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
            Ordering Please visit Ordering instruction.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            付加的提供条件 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            宮脇敦史博士による提供承諾書 [Word]
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08020 CS-RfA-ETV DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB08020z.seq
            Remarks, protocol and/or map (pdf) RDB08020.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12130_A2H6p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: bGH_rev3
            Region: bGHpA,3' LTR,PRE
            Sequence file: RDB12130_A2H6h.seq check
            >08020_12130_B0I2F_bGH_rev3_C02_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN GNNGGGNAAN AACAGATGGC TGGCAACTAG AAGGCACAGT
               61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG
              121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
              181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
              241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA
              301 TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT
              361 CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT
              421 CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC
              481 TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA
              541 TCCACAGATC AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT
              601 TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA
              661 CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT
              721 CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC
              781 GGTATCGATG CGGGGAGGCG GCCNNAAGGG AGATCCGANT CGNCTGANGG CGAANGCGAA
              841 NANGCGGAAN ANGCCGCANA NCCGGCANCN NNNCGCNGGA NNGAANGNCC GCTGGNNTGA
              901 NGGNCGAANG GNNGTNNNAN NNNGNNGNNC NNCGCANAAN NNNNNNGGNN NCNNAGGNNA
            961 NCANNCNNNN NNANNGNNNN NNNCNNNNNN NNANNNNNNN NNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,Zeo,SV40pA
            Sequence file: RDB12130_A2H6i.seq check
            >08020_12130_B0I2F_SV40pro_ori_F_D02_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCCNCTGCCT CTGANCTATT CNNGAAGTAG TGAGGAGGCT
               61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
              121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
              181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
              241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
              301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
              361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
              421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
              481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
              541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC
              601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT
              661 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC
              721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
              781 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC
              841 GTCNACCTCT AGCTAGANCT TGNCGTAATC ATGGTCATAN CTGTTTCCNG TGNGAAATTG
              901 TTATCNGCTC ACAATTCCNC ACNACATACN ANCNNGAAGC NTAAAGNNNA ANGCCNGGGG
            961 NNNNNNNANN ANNNNNCTNN NNNNNNTNAN NNNNNNNNN
            //
            Primer: EGFP-N
            Region: T2A,TetR
            Sequence file: RDB12130_A2H6j.seq check
            >8020_EGFP-N_D03_12.ab1
                1 NNNNNNNNNN NNNCNNNNCG CCNTTGCTNN NNNTGGGCCC GGGNNTTTCC TCCNCGTCAC
               61 CGCATGTTAG AAGACTTCCT CTGCCCTCGC CGGATCCGCT GTACGCGGAC CCACTTTCAC
              121 ATTTAAGTTG TTTTTCTAAT CCGCATATGA TCAATTCAAG GCCGAATAAG AAGGCTGGCT
              181 CTGCACCTTG GTGATCAAAT AATTCGATAG CTTGTCGTAA TAATGGCGGC ATACTATCAG
              241 TAGTAGGTGT TTCCCTTTCT TCTTTAGCGA CTTGATGCTC TTGATCTTCC AATACGCAAC
              301 CTAAAGTAAA ATGCCCCACA GCGCTGAGTG CATATAATGC ATTCTCTAGT GAAAAACCTT
              361 GTTGGCATAA AAAGGCTAAT TGATTTTCGA GAGTTTCATA CTGTTTTTCT GTAGGCCGTG
              421 TACCTAAATG TACTTTTGCT CCATCGCGAT GACTTAGTAA AGCACATCTA AAACTTTTAG
              481 CGTTATTACG TAAAAAATCT TGCCAGCTTT CCCCTTCTAA AGGGCAAAAG TGAGTATGGT
              541 GCCTATCTAA CATCTCAATG GCTAAGGCGT CGAGCAAAGC CCGCTTATTT TTTACATGCC
              601 AATACAATGT AGGCTGCTCT ACACCTAGCT TCTGGGCGAG TTTACGGGTT GTTAAACCTT
              661 CGATTCCGAC CTCATTAAGC AGCTCTAATG CGCTGTTAAT CACTTTACTT TNATCTAATC
              721 TAGACATGGT GGCGACCGGT AGCGCTAGTC TAGCTGCCAC CGCCACCTTA TGCGGCCGGT
              781 TAATTAATTC ACGACACCTG AAATGGAAGA AAAAAACTTT GAACCACTGT CTGANGCTTG
              841 NNAANGAACC AAGATCCNNA CTCAAAAAGG GCAAATTCCN AGGNNAANTA CATCAAGTGN
              901 NNAGCTGGNC NNACTTCANT CNCCANCCAC TCANNGNGGG GAANNTCCAT CGCATAAAAN
              961 CNNNNCNNCC NNNCNAAAGA NNANNNANTN NNAAANCTNN NNNNNTAATC NNNNNGNNNN
            1021 NNNNNN
            //
            Primer: PRE_rev
            Region: PRE,Venus 3'
            Sequence file: RDB12130_A2H6k.seq check
            >08020_12130_B0I2F_PRE_rev_B02_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNGNNCNNC ATAGTTAAGA ATACCAGTCA ATCTTTCNCA
               61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGTGC GGCCAATTTT
              121 TACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG
              181 ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGTA GCTCAGGTAG
              241 TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG
              301 GCGAGCTGCA CGCCGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTGGC CTTGATGCCG
              361 TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG
              421 TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC
              481 AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG
              541 AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC
              601 TTCATGTGGT CGGGGTAGCG GGCGAAGCAC TGCAGGCCGT AGCCCAGGGT GGTCACGAGG
              661 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATCA GCTTCAGGGT CAGCTTGCCG
              721 TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
              781 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
              841 NGCCCGGGAT TTTCCTCCAC GTCACCGCAN GTTANAANAC TTCCTCTGCC CTCGCCGGNT
              901 CCGCTGTACN CGGANCCACT TTNNCATTNN AGTTGNTTTT TNTANTCCNN ATATGNTCNA
            961 TTNNNNNNNN ANNNANANNN NNGGCNNNGC NNCNTNNNNN NNNNANNNNN NNNNN
            //
            Primer: CMV_Forward
            Region: CMV pro,5' LTR,HIV-1 psi
            Sequence file: RDB12130_A2H6l.seq check
            >8020_CMV-Forward_C03_09.ab1
                1 NNNNNNNNNN NNNNNNTTGN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
               61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
              121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
              181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
              241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
              301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
              361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
              421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
              481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
              541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
              601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
              661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
              721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGANCA CCGCACAGCA AGCGGCCGCT
              781 GATCTTCNGA CCTGGAGGAN GANNNATGAN GGACAATTGG AGAAGTGANT TATATAAATA
              841 TAAAGTAGTA AAAATTGNAC CNTTANGANT AGCACCCNCC AANGCAAAGA NAAGANTGGT
              901 GCAGANANAA AAAAGANCAN NGGGAATANG ANCTTTNNNN CTNGGGNTCN NGGNNNCANC
            961 NNNAANCACT ATNGNNNNNN NNNN
            //
            Primer: RRE-M_R
            Region: RRE,HIV-1 psi
            Sequence file: RDB12130_A2H6m.seq check
            >08020_12130_B0I2F_RRE-M_R_E02_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNGCTG TTGCGCCTCN NAGCCCTCNG CAAATTGTTC TGCTGCTGCA
               61 CTATACCAGA CAATAATTGT CTGGCCTGTA CCGTCAGCGT CATTGACGCT GCGCCCATAG
              121 TGCTTCCTGC TGCTCCCAAG AACCCAAGGA ACAAAGCTCC TATTCCCACT GCTCTTTTTT
              181 CTCTCTGCAC CACTCTTCTC TTTGCCTTGG TGGGTGCTAC TCCTAATGGT TCAATTTTTA
              241 CTACTTTATA TTTATATAAT TCACTTCTCC AATTGTCCCT CATATCTCCT CCTCCAGGTC
              301 TGAAGATCAG CGGCCGCTTG CTGTGCGGTG GTCTTACTTT TGTTTTGCTC TTCCTCTATC
              361 TTGTCTAAAG CTTCCTTGGT GTCTTTTATC TCTATCCTTT GATGCACACA ATAGAGGGTT
              421 GCTACTGTAT TATATAATGA TCTAAGTTCT TCTGATCCTG TCTGAAGGGA TGGTTGTAGC
              481 TGTCCCAGTA TTTGTCTACA GCCTTCTGAT GTTTCTAACA GGCCAGGATT AACTGCGAAT
              541 CGTTCTAGCT CCCTGCTTGC CCATACTATA TGTTTTAATT TATATTTTTT CTTTCCCCCT
              601 GGCCTTAACC GAATTTTTTC CCATCGCGAT CTAATTCTCC CCCGCTTAAT ACTGACGCTC
              661 TCGCACCCAT CTCTCTCCTT CTAGCCTCCG CTAGTCAAAA TTTTTGGCGT ACTCACCAGT
              721 CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT TCAGCAAGCC GAGTCCTGCG TCNAGAGAGC
              781 TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC CTGTTCGGNC GNNACTGCTA NAGNNTTTCC
              841 ACACTGACTA AAANNGTCTG ANGGATCTCT ANTTANCAGA GTCACACAAC AGANNGGCAC
              901 ACACTACTTG AAGCACTCNA NGNNAGCTTT ANTGNNNNNT TNNNNANNGG NNNCCNNANN
            961 TNANCCNGAN NNNNNCCANN NNCANNNNNN GNNNNNNNNN N
            //
            Primer: EGFP-M_R
            Region: Venus 5',T2A,TetR
            Sequence file: RDB12130_A2H6n.seq check
            >08020_12130_B0I2F_EGFP-M_R_A02_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGNNGGNNCT TGNNGTTGGC CTTGNTGCCG TTCTTCTGCT
               61 TGTCGGCGGT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
              121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
              181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
              241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
              301 CGGGGTAGCG GGCGAAGCAC TGCAGGCCGT AGCCCAGGGT GGTCACGAGG GTGGGCCAGG
              361 GCACGGGCAG CTTGCCGGTG GTGCAGATCA GCTTCAGGGT CAGCTTGCCG TAGGTGGCAT
              421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA
              481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GGCCCGGGAT
              541 TTTCCTCCAC GTCACCGCAT GTTAGAAGAC TTCCTCTGCC CTCGCCGGAT CCGCTGTACG
              601 CGGACCCACT TTCACATTTA AGTTGTTTTT CTAATCCGCA TATGATCAAT TCAAGGCCGA
              661 ATAAGAAGGC TGGCTCTGCA CCTTGGTGAT CAAATAATTC GATAGCTTGT CGTAATAATG
              721 GCGGCATACT ATCAGTAGTA GGTGTTTCCC TTTCTTCTTT AGCGACTTGA TGCTCTTGAT
              781 CTTCCAATAC GCAACCTAAA GTAAAATGCC CCACAGCGCT GANTGCATAT AATGCATTCT
              841 CTAGTGAAAA ACNTTGTTGG CATAAAAAGG CTAATTGANT TTCGAGAGTT TCATACNGNT
              901 TTTTCTGNNN NCNGNGNNNC NAANTGNACN TTTNGCNNNN TCNCNANGNN TTNNNAAAGC
              961 NNNTCTNAAA ANTTTTANNG NTNNTTNNNN NAAAAAANNN NTNNNNNNNN TTNNNNNNNN
            1021 NN
            //
            Primer: bGH_rev3
            Region: bGHpA,3' LTR,PRE
            Sequence file: RDB12130_A2H6o.seq check
            >08020_12130_B0I2F_bGH_rev3_C02_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN GNNGGGNAAN AACAGATGGC TGGCAACTAG AAGGCACAGT
               61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG
              121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
              181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
              241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA
              301 TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT
              361 CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT
              421 CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC
              481 TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA
              541 TCCACAGATC AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT
              601 TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA
              661 CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT
              721 CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC
              781 GGTATCGATG CGGGGAGGCG GCCNNAAGGG AGATCCGANT CGNCTGANGG CGAANGCGAA
              841 NANGCGGAAN ANGCCGCANA NCCGGCANCN NNNCGCNGGA NNGAANGNCC GCTGGNNTGA
              901 NGGNCGAANG GNNGTNNNAN NNNGNNGNNC NNCGCANAAN NNNNNNGGNN NCNNAGGNNA
            961 NCANNCNNNN NNANNGNNNN NNNCNNNNNN NNANNNNNNN NNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,Zeo,SV40pA
            Sequence file: RDB12130_A2H6p.seq check
            >08020_12130_B0I2F_SV40pro_ori_F_D02_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCCNCTGCCT CTGANCTATT CNNGAAGTAG TGAGGAGGCT
               61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
              121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
              181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
              241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
              301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
              361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
              421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
              481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
              541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC
              601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT
              661 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC
              721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
              781 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC
              841 GTCNACCTCT AGCTAGANCT TGNCGTAATC ATGGTCATAN CTGTTTCCNG TGNGAAATTG
              901 TTATCNGCTC ACAATTCCNC ACNACATACN ANCNNGAAGC NTAAAGNNNA ANGCCNGGGG
            961 NNNNNNNANN ANNNNNCTNN NNNNNNTNAN NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Noura, M., Pivotal role of DPYSL2A in KLF4-mediated monocytic differentiation of acute myeloid leukemia cells. Sci. Rep. 10 (1): 20245 (2020). PMID 33219287.
            user_report Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219.
            user_report Mitsuda, Y., RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci. Rep. 8 (1): 6423 (2018). PMID 29686309.
            user_report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
            user_report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
            user_report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Advances 1: 1440-1451 (2017). PMID 29296785.

            2022.05.23

            GNP_filter3_RDBDEP_html_220520.pl