Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-RfA-ETV

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Venus marker driven by EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 付加的提供条件： 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 宮脇敦史博士による提供承諾書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12130_A2H6p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: bGH_rev3 Region: bGHpA,3' LTR,PRE Sequence file: RDB12130_A2H6h.seq
>08020_12130_B0I2F_bGH_rev3_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNGGGNAAN AACAGATGGC TGGCAACTAG AAGGCACAGT    61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG   121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA   181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC   241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA   301 TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT   361 CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT   421 CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC   481 TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA   541 TCCACAGATC AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT   601 TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA   661 CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT   721 CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC   781 GGTATCGATG CGGGGAGGCG GCCNNAAGGG AGATCCGANT CGNCTGANGG CGAANGCGAA   841 NANGCGGAAN ANGCCGCANA NCCGGCANCN NNNCGCNGGA NNGAANGNCC GCTGGNNTGA   901 NGGNCGAANG GNNGTNNNAN NNNGNNGNNC NNCGCANAAN NNNNNNGGNN NCNNAGGNNA 961 NCANNCNNNN NNANNGNNNN NNNCNNNNNN NNANNNNNNN NNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori,Zeo,SV40pA Sequence file: RDB12130_A2H6i.seq
>08020_12130_B0I2F_SV40pro_ori_F_D02_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NCCNCTGCCT CTGANCTATT CNNGAAGTAG TGAGGAGGCT    61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT   121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA   181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG   241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT   301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA   361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT   421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT   481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA   541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC   601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT   661 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC   721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC   781 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC   841 GTCNACCTCT AGCTAGANCT TGNCGTAATC ATGGTCATAN CTGTTTCCNG TGNGAAATTG   901 TTATCNGCTC ACAATTCCNC ACNACATACN ANCNNGAAGC NTAAAGNNNA ANGCCNGGGG 961 NNNNNNNANN ANNNNNCTNN NNNNNNTNAN NNNNNNNNN //
Primer: EGFP-N Region: T2A,TetR Sequence file: RDB12130_A2H6j.seq
>8020_EGFP-N_D03_12.ab1     1 NNNNNNNNNN NNNCNNNNCG CCNTTGCTNN NNNTGGGCCC GGGNNTTTCC TCCNCGTCAC    61 CGCATGTTAG AAGACTTCCT CTGCCCTCGC CGGATCCGCT GTACGCGGAC CCACTTTCAC   121 ATTTAAGTTG TTTTTCTAAT CCGCATATGA TCAATTCAAG GCCGAATAAG AAGGCTGGCT   181 CTGCACCTTG GTGATCAAAT AATTCGATAG CTTGTCGTAA TAATGGCGGC ATACTATCAG   241 TAGTAGGTGT TTCCCTTTCT TCTTTAGCGA CTTGATGCTC TTGATCTTCC AATACGCAAC   301 CTAAAGTAAA ATGCCCCACA GCGCTGAGTG CATATAATGC ATTCTCTAGT GAAAAACCTT   361 GTTGGCATAA AAAGGCTAAT TGATTTTCGA GAGTTTCATA CTGTTTTTCT GTAGGCCGTG   421 TACCTAAATG TACTTTTGCT CCATCGCGAT GACTTAGTAA AGCACATCTA AAACTTTTAG   481 CGTTATTACG TAAAAAATCT TGCCAGCTTT CCCCTTCTAA AGGGCAAAAG TGAGTATGGT   541 GCCTATCTAA CATCTCAATG GCTAAGGCGT CGAGCAAAGC CCGCTTATTT TTTACATGCC   601 AATACAATGT AGGCTGCTCT ACACCTAGCT TCTGGGCGAG TTTACGGGTT GTTAAACCTT   661 CGATTCCGAC CTCATTAAGC AGCTCTAATG CGCTGTTAAT CACTTTACTT TNATCTAATC   721 TAGACATGGT GGCGACCGGT AGCGCTAGTC TAGCTGCCAC CGCCACCTTA TGCGGCCGGT   781 TAATTAATTC ACGACACCTG AAATGGAAGA AAAAAACTTT GAACCACTGT CTGANGCTTG   841 NNAANGAACC AAGATCCNNA CTCAAAAAGG GCAAATTCCN AGGNNAANTA CATCAAGTGN   901 NNAGCTGGNC NNACTTCANT CNCCANCCAC TCANNGNGGG GAANNTCCAT CGCATAAAAN   961 CNNNNCNNCC NNNCNAAAGA NNANNNANTN NNAAANCTNN NNNNNTAATC NNNNNGNNNN 1021 NNNNNN //
Primer: PRE_rev Region: PRE,Venus 3' Sequence file: RDB12130_A2H6k.seq
>08020_12130_B0I2F_PRE_rev_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNNCNNC ATAGTTAAGA ATACCAGTCA ATCTTTCNCA    61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGTGC GGCCAATTTT   121 TACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG   181 ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGTA GCTCAGGTAG   241 TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG   301 GCGAGCTGCA CGCCGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTGGC CTTGATGCCG   361 TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG   421 TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC   481 AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG   541 AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC   601 TTCATGTGGT CGGGGTAGCG GGCGAAGCAC TGCAGGCCGT AGCCCAGGGT GGTCACGAGG   661 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATCA GCTTCAGGGT CAGCTTGCCG   721 TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG   781 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG   841 NGCCCGGGAT TTTCCTCCAC GTCACCGCAN GTTANAANAC TTCCTCTGCC CTCGCCGGNT   901 CCGCTGTACN CGGANCCACT TTNNCATTNN AGTTGNTTTT TNTANTCCNN ATATGNTCNA 961 TTNNNNNNNN ANNNANANNN NNGGCNNNGC NNCNTNNNNN NNNNANNNNN NNNNN //
Primer: CMV_Forward Region: CMV pro,5' LTR,HIV-1 psi Sequence file: RDB12130_A2H6l.seq
>8020_CMV-Forward_C03_09.ab1     1 NNNNNNNNNN NNNNNNTTGN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT   121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA   181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA   241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA   301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG   361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT   421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG   481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG   541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA   601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT   661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT   721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGANCA CCGCACAGCA AGCGGCCGCT   781 GATCTTCNGA CCTGGAGGAN GANNNATGAN GGACAATTGG AGAAGTGANT TATATAAATA   841 TAAAGTAGTA AAAATTGNAC CNTTANGANT AGCACCCNCC AANGCAAAGA NAAGANTGGT   901 GCAGANANAA AAAAGANCAN NGGGAATANG ANCTTTNNNN CTNGGGNTCN NGGNNNCANC 961 NNNAANCACT ATNGNNNNNN NNNN //
Primer: RRE-M_R Region: RRE,HIV-1 psi Sequence file: RDB12130_A2H6m.seq
>08020_12130_B0I2F_RRE-M_R_E02_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNGCTG TTGCGCCTCN NAGCCCTCNG CAAATTGTTC TGCTGCTGCA    61 CTATACCAGA CAATAATTGT CTGGCCTGTA CCGTCAGCGT CATTGACGCT GCGCCCATAG   121 TGCTTCCTGC TGCTCCCAAG AACCCAAGGA ACAAAGCTCC TATTCCCACT GCTCTTTTTT   181 CTCTCTGCAC CACTCTTCTC TTTGCCTTGG TGGGTGCTAC TCCTAATGGT TCAATTTTTA   241 CTACTTTATA TTTATATAAT TCACTTCTCC AATTGTCCCT CATATCTCCT CCTCCAGGTC   301 TGAAGATCAG CGGCCGCTTG CTGTGCGGTG GTCTTACTTT TGTTTTGCTC TTCCTCTATC   361 TTGTCTAAAG CTTCCTTGGT GTCTTTTATC TCTATCCTTT GATGCACACA ATAGAGGGTT   421 GCTACTGTAT TATATAATGA TCTAAGTTCT TCTGATCCTG TCTGAAGGGA TGGTTGTAGC   481 TGTCCCAGTA TTTGTCTACA GCCTTCTGAT GTTTCTAACA GGCCAGGATT AACTGCGAAT   541 CGTTCTAGCT CCCTGCTTGC CCATACTATA TGTTTTAATT TATATTTTTT CTTTCCCCCT   601 GGCCTTAACC GAATTTTTTC CCATCGCGAT CTAATTCTCC CCCGCTTAAT ACTGACGCTC   661 TCGCACCCAT CTCTCTCCTT CTAGCCTCCG CTAGTCAAAA TTTTTGGCGT ACTCACCAGT   721 CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT TCAGCAAGCC GAGTCCTGCG TCNAGAGAGC   781 TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC CTGTTCGGNC GNNACTGCTA NAGNNTTTCC   841 ACACTGACTA AAANNGTCTG ANGGATCTCT ANTTANCAGA GTCACACAAC AGANNGGCAC   901 ACACTACTTG AAGCACTCNA NGNNAGCTTT ANTGNNNNNT TNNNNANNGG NNNCCNNANN 961 TNANCCNGAN NNNNNCCANN NNCANNNNNN GNNNNNNNNN N //
Primer: EGFP-M_R Region: Venus 5',T2A,TetR Sequence file: RDB12130_A2H6n.seq
>08020_12130_B0I2F_EGFP-M_R_A02_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNGGNNCT TGNNGTTGGC CTTGNTGCCG TTCTTCTGCT    61 TGTCGGCGGT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA   121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC   181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC   241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT   301 CGGGGTAGCG GGCGAAGCAC TGCAGGCCGT AGCCCAGGGT GGTCACGAGG GTGGGCCAGG   361 GCACGGGCAG CTTGCCGGTG GTGCAGATCA GCTTCAGGGT CAGCTTGCCG TAGGTGGCAT   421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA   481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GGCCCGGGAT   541 TTTCCTCCAC GTCACCGCAT GTTAGAAGAC TTCCTCTGCC CTCGCCGGAT CCGCTGTACG   601 CGGACCCACT TTCACATTTA AGTTGTTTTT CTAATCCGCA TATGATCAAT TCAAGGCCGA   661 ATAAGAAGGC TGGCTCTGCA CCTTGGTGAT CAAATAATTC GATAGCTTGT CGTAATAATG   721 GCGGCATACT ATCAGTAGTA GGTGTTTCCC TTTCTTCTTT AGCGACTTGA TGCTCTTGAT   781 CTTCCAATAC GCAACCTAAA GTAAAATGCC CCACAGCGCT GANTGCATAT AATGCATTCT   841 CTAGTGAAAA ACNTTGTTGG CATAAAAAGG CTAATTGANT TTCGAGAGTT TCATACNGNT   901 TTTTCTGNNN NCNGNGNNNC NAANTGNACN TTTNGCNNNN TCNCNANGNN TTNNNAAAGC   961 NNNTCTNAAA ANTTTTANNG NTNNTTNNNN NAAAAAANNN NTNNNNNNNN TTNNNNNNNN 1021 NN //
Primer: bGH_rev3 Region: bGHpA,3' LTR,PRE Sequence file: RDB12130_A2H6o.seq
>08020_12130_B0I2F_bGH_rev3_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNGGGNAAN AACAGATGGC TGGCAACTAG AAGGCACAGT    61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG   121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA   181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC   241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA   301 TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT   361 CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT   421 CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC   481 TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA   541 TCCACAGATC AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT   601 TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA   661 CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT   721 CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC   781 GGTATCGATG CGGGGAGGCG GCCNNAAGGG AGATCCGANT CGNCTGANGG CGAANGCGAA   841 NANGCGGAAN ANGCCGCANA NCCGGCANCN NNNCGCNGGA NNGAANGNCC GCTGGNNTGA   901 NGGNCGAANG GNNGTNNNAN NNNGNNGNNC NNCGCANAAN NNNNNNGGNN NCNNAGGNNA 961 NCANNCNNNN NNANNGNNNN NNNCNNNNNN NNANNNNNNN NNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori,Zeo,SV40pA Sequence file: RDB12130_A2H6p.seq
>08020_12130_B0I2F_SV40pro_ori_F_D02_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NCCNCTGCCT CTGANCTATT CNNGAAGTAG TGAGGAGGCT    61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT   121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA   181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG   241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT   301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA   361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT   421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT   481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA   541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC   601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT   661 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC   721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC   781 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC   841 GTCNACCTCT AGCTAGANCT TGNCGTAATC ATGGTCATAN CTGTTTCCNG TGNGAAATTG   901 TTATCNGCTC ACAATTCCNC ACNACATACN ANCNNGAAGC NTAAAGNNNA ANGCCNGGGG 961 NNNNNNNANN ANNNNNCTNN NNNNNNTNAN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.10.12

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