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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CS-RfA-ETV (#RDB08020)

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Venus marker driven by EF-1 alpha promoter.

Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Venus marker driven by EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CS-RfA-ETV ()
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB08020z.seq
Remarks, protocol and/or map (pdf) RDB08020.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08020 CS-RfA-ETV DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-RfA-ETV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08020).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12130_A2H6p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: bGH_rev3 (Pr0885)
Region: bGHpA,3' LTR,PRE
Sequence file: RDB12130_A2H6h.seq check
>08020_12130_B0I2F_bGH_rev3_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNGGGNAAN AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG
  121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
  181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
  241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA
  301 TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT
  361 CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT
  421 CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC
  481 TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA
  541 TCCACAGATC AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT
  601 TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA
  661 CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT
  721 CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC
  781 GGTATCGATG CGGGGAGGCG GCCNNAAGGG AGATCCGANT CGNCTGANGG CGAANGCGAA
  841 NANGCGGAAN ANGCCGCANA NCCGGCANCN NNNCGCNGGA NNGAANGNCC GCTGGNNTGA
  901 NGGNCGAANG GNNGTNNNAN NNNGNNGNNC NNCGCANAAN NNNNNNGGNN NCNNAGGNNA
961 NCANNCNNNN NNANNGNNNN NNNCNNNNNN NNANNNNNNN NNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,Zeo,SV40pA
Sequence file: RDB12130_A2H6i.seq check
>08020_12130_B0I2F_SV40pro_ori_F_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCCNCTGCCT CTGANCTATT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
  121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
  181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
  241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
  301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
  361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
  421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
  481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
  541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC
  601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT
  661 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC
  721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  781 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC
  841 GTCNACCTCT AGCTAGANCT TGNCGTAATC ATGGTCATAN CTGTTTCCNG TGNGAAATTG
  901 TTATCNGCTC ACAATTCCNC ACNACATACN ANCNNGAAGC NTAAAGNNNA ANGCCNGGGG
961 NNNNNNNANN ANNNNNCTNN NNNNNNTNAN NNNNNNNNN
//
Primer: EGFP-N (Pr0027)
Region: T2A,TetR
Sequence file: RDB12130_A2H6j.seq check
>8020_EGFP-N_D03_12.ab1
    1 NNNNNNNNNN NNNCNNNNCG CCNTTGCTNN NNNTGGGCCC GGGNNTTTCC TCCNCGTCAC
   61 CGCATGTTAG AAGACTTCCT CTGCCCTCGC CGGATCCGCT GTACGCGGAC CCACTTTCAC
  121 ATTTAAGTTG TTTTTCTAAT CCGCATATGA TCAATTCAAG GCCGAATAAG AAGGCTGGCT
  181 CTGCACCTTG GTGATCAAAT AATTCGATAG CTTGTCGTAA TAATGGCGGC ATACTATCAG
  241 TAGTAGGTGT TTCCCTTTCT TCTTTAGCGA CTTGATGCTC TTGATCTTCC AATACGCAAC
  301 CTAAAGTAAA ATGCCCCACA GCGCTGAGTG CATATAATGC ATTCTCTAGT GAAAAACCTT
  361 GTTGGCATAA AAAGGCTAAT TGATTTTCGA GAGTTTCATA CTGTTTTTCT GTAGGCCGTG
  421 TACCTAAATG TACTTTTGCT CCATCGCGAT GACTTAGTAA AGCACATCTA AAACTTTTAG
  481 CGTTATTACG TAAAAAATCT TGCCAGCTTT CCCCTTCTAA AGGGCAAAAG TGAGTATGGT
  541 GCCTATCTAA CATCTCAATG GCTAAGGCGT CGAGCAAAGC CCGCTTATTT TTTACATGCC
  601 AATACAATGT AGGCTGCTCT ACACCTAGCT TCTGGGCGAG TTTACGGGTT GTTAAACCTT
  661 CGATTCCGAC CTCATTAAGC AGCTCTAATG CGCTGTTAAT CACTTTACTT TNATCTAATC
  721 TAGACATGGT GGCGACCGGT AGCGCTAGTC TAGCTGCCAC CGCCACCTTA TGCGGCCGGT
  781 TAATTAATTC ACGACACCTG AAATGGAAGA AAAAAACTTT GAACCACTGT CTGANGCTTG
  841 NNAANGAACC AAGATCCNNA CTCAAAAAGG GCAAATTCCN AGGNNAANTA CATCAAGTGN
  901 NNAGCTGGNC NNACTTCANT CNCCANCCAC TCANNGNGGG GAANNTCCAT CGCATAAAAN
  961 CNNNNCNNCC NNNCNAAAGA NNANNNANTN NNAAANCTNN NNNNNTAATC NNNNNGNNNN
1021 NNNNNN
//
Primer: PRE_rev (Pr0250)
Region: PRE,Venus 3'
Sequence file: RDB12130_A2H6k.seq check
>08020_12130_B0I2F_PRE_rev_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGNNCNNC ATAGTTAAGA ATACCAGTCA ATCTTTCNCA
   61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGTGC GGCCAATTTT
  121 TACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG
  181 ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGTA GCTCAGGTAG
  241 TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG
  301 GCGAGCTGCA CGCCGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTGGC CTTGATGCCG
  361 TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG
  421 TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC
  481 AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG
  541 AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC
  601 TTCATGTGGT CGGGGTAGCG GGCGAAGCAC TGCAGGCCGT AGCCCAGGGT GGTCACGAGG
  661 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATCA GCTTCAGGGT CAGCTTGCCG
  721 TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
  781 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
  841 NGCCCGGGAT TTTCCTCCAC GTCACCGCAN GTTANAANAC TTCCTCTGCC CTCGCCGGNT
  901 CCGCTGTACN CGGANCCACT TTNNCATTNN AGTTGNTTTT TNTANTCCNN ATATGNTCNA
961 TTNNNNNNNN ANNNANANNN NNGGCNNNGC NNCNTNNNNN NNNNANNNNN NNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,5' LTR,HIV-1 psi
Sequence file: RDB12130_A2H6l.seq check
>8020_CMV-Forward_C03_09.ab1
    1 NNNNNNNNNN NNNNNNTTGN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
  121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
  181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
  241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
  301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
  361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
  421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
  481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
  541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
  601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
  661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
  721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGANCA CCGCACAGCA AGCGGCCGCT
  781 GATCTTCNGA CCTGGAGGAN GANNNATGAN GGACAATTGG AGAAGTGANT TATATAAATA
  841 TAAAGTAGTA AAAATTGNAC CNTTANGANT AGCACCCNCC AANGCAAAGA NAAGANTGGT
  901 GCAGANANAA AAAAGANCAN NGGGAATANG ANCTTTNNNN CTNGGGNTCN NGGNNNCANC
961 NNNAANCACT ATNGNNNNNN NNNN
//
Primer: RRE-M_R (Pr0651)
Region: RRE,HIV-1 psi
Sequence file: RDB12130_A2H6m.seq check
>08020_12130_B0I2F_RRE-M_R_E02_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGCTG TTGCGCCTCN NAGCCCTCNG CAAATTGTTC TGCTGCTGCA
   61 CTATACCAGA CAATAATTGT CTGGCCTGTA CCGTCAGCGT CATTGACGCT GCGCCCATAG
  121 TGCTTCCTGC TGCTCCCAAG AACCCAAGGA ACAAAGCTCC TATTCCCACT GCTCTTTTTT
  181 CTCTCTGCAC CACTCTTCTC TTTGCCTTGG TGGGTGCTAC TCCTAATGGT TCAATTTTTA
  241 CTACTTTATA TTTATATAAT TCACTTCTCC AATTGTCCCT CATATCTCCT CCTCCAGGTC
  301 TGAAGATCAG CGGCCGCTTG CTGTGCGGTG GTCTTACTTT TGTTTTGCTC TTCCTCTATC
  361 TTGTCTAAAG CTTCCTTGGT GTCTTTTATC TCTATCCTTT GATGCACACA ATAGAGGGTT
  421 GCTACTGTAT TATATAATGA TCTAAGTTCT TCTGATCCTG TCTGAAGGGA TGGTTGTAGC
  481 TGTCCCAGTA TTTGTCTACA GCCTTCTGAT GTTTCTAACA GGCCAGGATT AACTGCGAAT
  541 CGTTCTAGCT CCCTGCTTGC CCATACTATA TGTTTTAATT TATATTTTTT CTTTCCCCCT
  601 GGCCTTAACC GAATTTTTTC CCATCGCGAT CTAATTCTCC CCCGCTTAAT ACTGACGCTC
  661 TCGCACCCAT CTCTCTCCTT CTAGCCTCCG CTAGTCAAAA TTTTTGGCGT ACTCACCAGT
  721 CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT TCAGCAAGCC GAGTCCTGCG TCNAGAGAGC
  781 TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC CTGTTCGGNC GNNACTGCTA NAGNNTTTCC
  841 ACACTGACTA AAANNGTCTG ANGGATCTCT ANTTANCAGA GTCACACAAC AGANNGGCAC
  901 ACACTACTTG AAGCACTCNA NGNNAGCTTT ANTGNNNNNT TNNNNANNGG NNNCCNNANN
961 TNANCCNGAN NNNNNCCANN NNCANNNNNN GNNNNNNNNN N
//
Primer: EGFP-M_R (Pr0743)
Region: Venus 5',T2A,TetR
Sequence file: RDB12130_A2H6n.seq check
>08020_12130_B0I2F_EGFP-M_R_A02_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNGGNNCT TGNNGTTGGC CTTGNTGCCG TTCTTCTGCT
   61 TGTCGGCGGT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
  121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
  181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
  241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
  301 CGGGGTAGCG GGCGAAGCAC TGCAGGCCGT AGCCCAGGGT GGTCACGAGG GTGGGCCAGG
  361 GCACGGGCAG CTTGCCGGTG GTGCAGATCA GCTTCAGGGT CAGCTTGCCG TAGGTGGCAT
  421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA
  481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GGCCCGGGAT
  541 TTTCCTCCAC GTCACCGCAT GTTAGAAGAC TTCCTCTGCC CTCGCCGGAT CCGCTGTACG
  601 CGGACCCACT TTCACATTTA AGTTGTTTTT CTAATCCGCA TATGATCAAT TCAAGGCCGA
  661 ATAAGAAGGC TGGCTCTGCA CCTTGGTGAT CAAATAATTC GATAGCTTGT CGTAATAATG
  721 GCGGCATACT ATCAGTAGTA GGTGTTTCCC TTTCTTCTTT AGCGACTTGA TGCTCTTGAT
  781 CTTCCAATAC GCAACCTAAA GTAAAATGCC CCACAGCGCT GANTGCATAT AATGCATTCT
  841 CTAGTGAAAA ACNTTGTTGG CATAAAAAGG CTAATTGANT TTCGAGAGTT TCATACNGNT
  901 TTTTCTGNNN NCNGNGNNNC NAANTGNACN TTTNGCNNNN TCNCNANGNN TTNNNAAAGC
  961 NNNTCTNAAA ANTTTTANNG NTNNTTNNNN NAAAAAANNN NTNNNNNNNN TTNNNNNNNN
1021 NN
//
Primer: bGH_rev3 (Pr0885)
Region: bGHpA,3' LTR,PRE
Sequence file: RDB12130_A2H6o.seq check
>08020_12130_B0I2F_bGH_rev3_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNGGGNAAN AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG
  121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
  181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
  241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA
  301 TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT
  361 CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT
  421 CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC
  481 TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA
  541 TCCACAGATC AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT
  601 TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA
  661 CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT
  721 CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC
  781 GGTATCGATG CGGGGAGGCG GCCNNAAGGG AGATCCGANT CGNCTGANGG CGAANGCGAA
  841 NANGCGGAAN ANGCCGCANA NCCGGCANCN NNNCGCNGGA NNGAANGNCC GCTGGNNTGA
  901 NGGNCGAANG GNNGTNNNAN NNNGNNGNNC NNCGCANAAN NNNNNNGGNN NCNNAGGNNA
961 NCANNCNNNN NNANNGNNNN NNNCNNNNNN NNANNNNNNN NNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,Zeo,SV40pA
Sequence file: RDB12130_A2H6p.seq check
>08020_12130_B0I2F_SV40pro_ori_F_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCCNCTGCCT CTGANCTATT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
  121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
  181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
  241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
  301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
  361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
  421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
  481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
  541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC
  601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT
  661 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC
  721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  781 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC
  841 GTCNACCTCT AGCTAGANCT TGNCGTAATC ATGGTCATAN CTGTTTCCNG TGNGAAATTG
  901 TTATCNGCTC ACAATTCCNC ACNACATACN ANCNNGAAGC NTAAAGNNNA ANGCCNGGGG
961 NNNNNNNANN ANNNNNCTNN NNNNNNTNAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Noura, M., Suppression of super-enhancer-driven TAL1 expression by KLF4 in T-cell acute lymphoblastic leukemia.
29 June 2023, PREPRINT (Version 1) available at Research Square https://doi.org/10.21203/rs.3.rs-3069900/v1
user_report Watanabe, K., Inhibition of the galactosyltransferase C1GALT1 reduces osteosarcoma cell proliferation by interfering with ERK signaling and cell cycle progression. Cancer Gene Ther. 2024 Apr 15. doi: 10.1038/s41417-024-00773-9. Epub ahead of print. PMID 38622340. [link to RRC of NBRP]
user_report Matsui, Y., Chlorambucil-conjugated PI-polyamides (Chb-M'), a transcription inhibitor of RUNX family, has an anti-tumor activity against SHH-type medulloblastoma with p53 mutation. Biochem. Biophys. Res. Commun. 620: 150-157 (2022). PMID 35792512. [link to RRC of NBRP]
user_report Hattori, E.Y., A RUNX-targeted gene switch-off approach modulates the BIRC5/PIF1-p21 pathway and reduces glioblastoma growth in mice. Commun. Biol. 5 (1): 939 (2022). PMID 36085167. [link to RRC of NBRP]
user_report Noura, M., Pivotal role of DPYSL2A in KLF4-mediated monocytic differentiation of acute myeloid leukemia cells. Sci. Rep. 10 (1): 20245 (2020). PMID 33219287. [link to RRC of NBRP]
user_report Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219. [link to RRC of NBRP]
user_report Mitsuda, Y., RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci. Rep. 8 (1): 6423 (2018). PMID 29686309. [link to RRC of NBRP]
user_report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640. [link to RRC of NBRP]
user_report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243. [link to RRC of NBRP]
user_report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Adv. 1: 1440-1451 (2017). PMID 29296785. [link to RRC of NBRP]