Bacterial expression vector of Thermoplasma volcanium Fur
Clone info. | Bacterial expression vector of Thermoplasma volcanium Fur. A gene encoded Ferric uptake regulator (Fur) of an archaeon, Thermoplasma volcanium GSS1 was ligated into EcoRI/NdeI sites of E. coli expression vector, pET28a(+). |
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Comment | The protein expression was comfirmed by the Depositor with BL21(DE3) adding 400 uM IPTG in the culture medium. |
Vector backbone | pET28a(+) (Plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Kan^r |
Gene/insert name | Thermoplasma volcanium TvFur (TVN0292) cDNA |
Depositor|Developer | Kawashima, Tsuyoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件クローンの使用は学術研究に限る。営利目的に使用してはならない。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB07959 | pET28a-TvFur | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pET28a-TvFur was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07959). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
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