RDB07951 - pMOS-PYPAF1

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icon below as well as clone information before placing your order.

pMOS-PYPAF1 (#RDB07951)

Plasmid vector of human PYPAF1

Clone info.	Plasmid vector of human PYPAF1. cDNA fragment 747nt to 3857nt of NM_004895.4 is cloned.
Comment	Known differences (at least) : T to C substitution at 905nt (Asp, synonym) ; C to T substitution at 1409nt (Thr, synonym).
Vector backbone	pMOSBlue (Plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Amp^r
Gene/insert name	Human PYPAF1 (NLRP3) cDNA
Depositor\|Developer	Suda, Takashi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Int. Immunol., 16, 777-786 (2004)). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name, his/her affiliation and the postal address. RECIPIENT shall contact and negotiate with the DEPOSITOR in the case of application for any patents and/or commercial use of the BIOLOGICAL RESOURCE and/or based on the results from the use of the BIOLOGICAL RESOURCE, with exception of use of the BIOLOGICAL RESOURCE for the research and development performed in the RECIPIENT's affiliation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Int. Immunol., 16, 777-786 (2004)). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name, his/her affiliation and the postal address. RECIPIENT shall contact and negotiate with the DEPOSITOR in the case of application for any patents and/or commercial use of the BIOLOGICAL RESOURCE and/or based on the results from the use of the BIOLOGICAL RESOURCE, with exception of use of the BIOLOGICAL RESOURCE for the research and development performed in the RECIPIENT's affiliation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Int. Immunol., 16, 777-786 (2004))。寄託者から要請がある場合、理研ＢＲＣは、利用者情報のうち、研究責任者の氏名及びその所属機関名並びに所在地を寄託者に通知するものとする。利用者が、本件リソース及び本件リソースを利用した研究成果に基づき、特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡し、その取扱いについて協議するものとする。ただし、利用者が所属する組織の研究開発のための本件リソースの利用を除くものとする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Int. Immunol., 16, 777-786 (2004))。寄託者から要請がある場合、理研ＢＲＣは、利用者情報のうち、研究責任者の氏名及びその所属機関名並びに所在地を寄託者に通知するものとする。利用者が、本件リソース及び本件リソースを利用した研究成果に基づき、特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡し、その取扱いについて協議するものとする。ただし、利用者が所属する組織の研究開発のための本件リソースの利用を除くものとする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07951	pMOS-PYPAF1	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMOS-PYPAF1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07951).

Reference section:

Wang, Y., Hasegawa, M., Imamura, R., Kinoshita, T., Kondo, C., Konaka, K., Suda, T., PYNOD, a novel Apaf-1/CED4-like protein is an inhibitor of ASC and caspase-1. Int. Immunol., 16, 777-786 (2004). PMID 15096476. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07951_A3Lpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB07951_A3Lpa.seq
>07951_07951_A3Lp_M13_-40_C01_03_ABI08.ab1 1 CCAACAGGGG AATCGAGGCT CGGTACCCGG GGATCCGATA CAGCTGCAGA TGAAGATGGC 61 AAGCACCCGC TGCAAGCTGG CCAGGTACCT GGAGGACCTG GAGGATGTGG ACTTGAAGAA 121 ATTTAAGATG CACTTAGAGG ACTATCCTCC CCAGAAGGGC TGCATCCCCC TCCCGAGGGG 181 TCAGACAGAG AAGGCAGACC ATGTGGACCT AGCCACGCTA ATGATCGACT TCAATGGGGA 241 GGAGAAGGCG TGGGCCATGG CCGTGTGGAT CTTCGCTGCG ATCAACAGGA GAGACCTTTA 301 TGAGAAAGCA AAAAGAGATG AGCCGAAGTG GGGTTCAGAT AATGCACGTG TTTCGAATCC 361 CACTGTGATA TGCCAGGAAG ACAGCATTGA AGAGGAGTGG ATGGGTTTAC TGGAGTACCT 421 TTCGAGAATC TCTATTTGTA AAATGAAGAA AGATTACCGT AAGAAGTACA GAAAGTACGT 481 GAGAAGCAGA TTCCAGTGCA TTGAAGACAG GAATGCCCGT CTGGGTGAGA GTGTGAGCCT 541 CAACAAACGC TACACACGAC TGCGTCTCAT CAAGGAGCAC CGGAGCCAGC AGGAGAGGGA 601 GCAGGAGCTT CTGGCCATCG GCAAGACCAA GACGTGTGAG AGCCCCGTGA GTCCCATTAA 661 GATGGAGTTG CTGTTTGACC CCGATGATGA GCATTCTGAG CCTGTGCACA CTGTGGTGTT 721 CCAGGGGGCG GCAGGGATTG GGAAAACAAT CCTGGCCAGG AAGATGATGT TGGACTGGGC 781 GTCGGGGACA CTCTACCAAG ACAGGTTTGA CTATCTGTTC TATATCCACT GTCGGGAGGT 841 GAGCCTTGTG ACACAGAGGA GCCTGGGGGA CCTGATCATG AGCTGCTGCC CCGACCCAAA 901 CCCACCCATC CACAAGATCG TGAGAAAACC CTCCAGAATC CTCTTCCTCA TGGACGGCTT 961 CGATGAGCTG CAAGGTGCCT TTTGACGAGC ACATAGGACC GCTCTGCACT GACTGGGCAG 1021 AGCCGGAGCG GGGAGAACAT TTCTCCTGAG CAGGCCTCAA TCAGGAAAAG AAGGCCTTGC 1081 TTCCCGAAGG TCCCTCTCTG CCTTCAATCA CCAACGTAG //
Primer: Reverse2 Sequence file: RDB07951_A3Lpb.seq
>07951_07951_A3Lp_Reverse2_D01_04_ABI08.ab1 1 CTAATCATTA CGCCAGCTCT AATACGACTC ACTATAGGGA AAGCTTGCAT GCCTGCAGGT 61 CGACTCTAGA GGATCTACTA GTCATATGGA TCCTACCAAG AAGGCTCAAA GACGACGGTC 121 AGCTCAGGCT TTTCTTCTTG AAGTGTTTCT AACGCACTTT TTGTCTCATA ATTGAAATAC 181 ATTTCAGACA ACCCCAGGTT CTGCAGGAGG CAGCTCTGCT GTTTCAGCAC TTCACAGAAC 241 ATCATGACCC CCAGGTCGCC CAGGTCATTG TTGCCCAGGC TCAGCTTTCG CAGGCTCTGG 301 CTGGAGGTCA GAAGTGTGGA AAGATCCCAG CAGCAGTGTG ACGTGAGGTT GCAGTTGTCT 361 AATTCCAACA CCTGAAGCTT GCAGTCGGGG TGCAAGAGTC CCTCACAGAG TAGTTTGATC 421 CCCTTGTCTC CGAGAGTGTT GCCTCGCAGG TAAAGGTGCG TGAGATTCTG ATTAGTGCTG 481 AGTACCGAGG ACAAAGCTGA ACAACAGACT GACGTAAGGC CAGAATTCAC CAACCCCAGT 541 TTCTGCAGGT TACACTGTGG ATTCTTGGCT TTTTCACATA AAATTGCGAC TCCTGAGTCT 601 CCCAAGGCAT TCTCCCCCAC ATAGAGTCTG GTCAGGGAAT GGCTGGTGCT CAATACTGAT 661 GCAAGATCCT GACAACATGC TGATGTGAGG CAGCAGCTGA CCAACCAGAG CTTCTTCAGA 721 TTGCACAACA GGTGCTTCAG TCCCACACAC AGAAGTCTGA TTCCGAAGTC ACCGAGGGCG 781 TTGTCACTCA GGTCCAGCTC CACCAGCTTC TGGTTGCTGC TGAGGACCAA GGAGATGTCG 841 AAGCAGCACT CATGCGAGAG GCCACAGCGC CCCAACCACA ATCTCCGAAT GTTACAGCCA 901 GGATGCTGGA GCGTTTCACA CAACACTCTC ATCCCTGGGT CCCCCAGAGA AATTGTCACT 961 GAGGTCCAAA TTCAGTTTAG ACTCTGGGCT GGGTGCTCAG AACTGAAAAG AGGCCCCCGG 1021 CAAAAAACTG GCAGTGAGGT GGCTGTTTCA CCGATCAATG GAGACAGGCA GCCATGAGAG 1081 AAGCCTGGAA GGACACACTG CACCATTATT CAAAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Wang, Y., PYNOD, a novel Apaf-1/CED4-like protein is an inhibitor of ASC and caspase-1. Int. Immunol., 16, 777-786 (2004). PMID 15096476. [link to RRC of NBRP]

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Other resources in our bank

human PYPAF1 (NLRP3)

External Database
Human PYPAF1 (NLRP3)

Reference sequence