Resource data sheet
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pEF-BOS-EX

Mammalian expression vector with human EF-1a.

Catalog number RDB07940
Resource name pEF-BOS-EX
Clone info. Mammalian expression vector with human EF-1a. Related vector pEF-BOS-EX (RDB07940) is also available.
Please refer Prof. Nagata's web page for datails (http://biochemi.ifrec.osaka-u.ac.jp/english/research/pef-bosex.html).
Vector backbone pEF-BOS-EX (Plasmid)
Size of vector backbone 5.277 bp
Selectable markers Amp^r
Depositor Nagata, Shigekazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07940 pEF-BOS-EX DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07940z.seq from Depositor
Electronic file PDF RDB07940.pdf from Depositor

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07940_A6Cmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB07940_A6Cma.seq
>07940_07940_A6Cm_EF1a-C_F_F03_18.ab1
    1 CCGTTGGGAA TTGCCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT
   61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA ATTCGGTACC GCGGCCGCCC
  121 GGGGATCCGC TAGCGTCGAC TCTAGAGTGA GGGTCCCCAC CTGGGACCCT TGAGAGTATC
  181 AGGTCTCCCA CGTGGGAGAC AAGAAATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCCA
  241 TCTGGGTCCT TGCACCCCTC ACTCTGGCCT CAGCCGACTG CACAGCGGCC CCTGCATCCC
  301 CTTGGCTGTG AGGCCCCTGG ACAAGCAGAG GTGGCCAGAG CTGGGAGGCA TGGCCCTGGG
  361 GTCCCACGAA TTTGCTGGGG AATCTCGTTT TTCTTCTTAA GACTTTTGGG ACATGGTTTG
  421 ACTCCCGAAC ATCACCGACG TGTCTCCTGT TTTTCTGGGT GGCCTCGGGA CACCTGCCCT
  481 GCCCCCACGA GGGTCAGGAC TGTGACTCTT TTTAGGGCCA GGCAGGTGCC TGGACATTTG
  541 CCTTGCTGGA CGGGGACTGG GGATGTGGGA GGGAGCAGAC AGGAGGAATC ATGTCAGGCC
  601 TGTGTGTGAA AGGAAGCTCC ACTGTCACCC TCCACCTCTT CACCCCCCAC TCACCAGTGT
  661 CCCCTCCACT GTCACATTGT AACTGAACTT CAGGATAATA AAGTGTTTGC CTCCAAAAAA
  721 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAGATAA ATAAAAAAAA AAAAAAAATA
  781 AAAAAAAAAA CCCAATTTTC CCGGGCCGTC TTTTACAACG TCGTGACTGG GAAAACCCTG
  841 GCGTTACCCA ACTTAATCGC CTTGCACCAA TCCCCCTTTC GCCAGCTGGC GTAAAAGCGA
  901 AAAAGCCCGC ACGGATCGCC CTTCCCAACA GTTGCGCAAC CTGAATGGCA AATGGCGCCT
  961 GATGCGGTAT TTTCTCCTTT ACGCATTCTG TGCGATATTA CACACCGGCA TACGTTCAAA
 1021 GGCATCCTAT AGTACGCGCC CTGGTAAGCG CGCATAAAGC GCCGCGATGT AGTGTAACGC
 1081 CAACGTGACC GCTTAACCTT GCAAGCCTTA GCCCGCTCAT GCATCTACAT CTACGCAGTT
1141 GCCGTTCCGT CAGCATTAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283.
reference Yanagihashi, Y., Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis. Proc. Natl. Acad. Sci. U.S.A. 114 (33): 8800-8805 (2017). PMID 28768810.

2018.12.10

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