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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-BOS-EX

Mammalian expression vector with human EF-1a.

Catalog number RDB07940
Resource name pEF-BOS-EX
Clone info. Mammalian expression vector with human EF-1a. Related vector pEF-BOS-EX (RDB07940) is also available.
Comment Please refer Prof. Nagata's web page for datails (http://biochemi.ifrec.osaka-u.ac.jp/english/research/pef-bosex.html).
Vector backbone pEF-BOS-EX (Plasmid)
Size of vector backbone 5.277 bp
Selectable markers Amp^r
Depositor|Developer Nagata, Shigekazu |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって、長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB07940 pEF-BOS-EX DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB07940z.seq
            Remarks, protocol and/or map (pdf) RDB07940.pdf
            Remarks, protocol and/or map (png) RDB07940_map.png

            Original reference

            original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB07940_A6Cmp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F
            Region: EF-1apro,MCS,hG-CSFp(A)
            Sequence file: RDB07940_A6Cma.seq check
            >07940_07940_A6Cm_EF1a-C_F_F03_18.ab1
                1 CCGTTGGGAA TTGCCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT
               61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA ATTCGGTACC GCGGCCGCCC
              121 GGGGATCCGC TAGCGTCGAC TCTAGAGTGA GGGTCCCCAC CTGGGACCCT TGAGAGTATC
              181 AGGTCTCCCA CGTGGGAGAC AAGAAATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCCA
              241 TCTGGGTCCT TGCACCCCTC ACTCTGGCCT CAGCCGACTG CACAGCGGCC CCTGCATCCC
              301 CTTGGCTGTG AGGCCCCTGG ACAAGCAGAG GTGGCCAGAG CTGGGAGGCA TGGCCCTGGG
              361 GTCCCACGAA TTTGCTGGGG AATCTCGTTT TTCTTCTTAA GACTTTTGGG ACATGGTTTG
              421 ACTCCCGAAC ATCACCGACG TGTCTCCTGT TTTTCTGGGT GGCCTCGGGA CACCTGCCCT
              481 GCCCCCACGA GGGTCAGGAC TGTGACTCTT TTTAGGGCCA GGCAGGTGCC TGGACATTTG
              541 CCTTGCTGGA CGGGGACTGG GGATGTGGGA GGGAGCAGAC AGGAGGAATC ATGTCAGGCC
              601 TGTGTGTGAA AGGAAGCTCC ACTGTCACCC TCCACCTCTT CACCCCCCAC TCACCAGTGT
              661 CCCCTCCACT GTCACATTGT AACTGAACTT CAGGATAATA AAGTGTTTGC CTCCAAAAAA
              721 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAGATAA ATAAAAAAAA AAAAAAAATA
              781 AAAAAAAAAA CCCAATTTTC CCGGGCCGTC TTTTACAACG TCGTGACTGG GAAAACCCTG
              841 GCGTTACCCA ACTTAATCGC CTTGCACCAA TCCCCCTTTC GCCAGCTGGC GTAAAAGCGA
              901 AAAAGCCCGC ACGGATCGCC CTTCCCAACA GTTGCGCAAC CTGAATGGCA AATGGCGCCT
              961 GATGCGGTAT TTTCTCCTTT ACGCATTCTG TGCGATATTA CACACCGGCA TACGTTCAAA
             1021 GGCATCCTAT AGTACGCGCC CTGGTAAGCG CGCATAAAGC GCCGCGATGT AGTGTAACGC
             1081 CAACGTGACC GCTTAACCTT GCAAGCCTTA GCCCGCTCAT GCATCTACAT CTACGCAGTT
            1141 GCCGTTCCGT CAGCATTAT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283.
            reference Yanagihashi, Y., Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis. Proc. Natl. Acad. Sci. U.S.A. 114 (33): 8800-8805 (2017). PMID 28768810.
            reference Murai, K., Myeloid-specific transcriptional activation by murine myeloid zinc-finger protein 2. Proc. Natl. Acad. Sci. U.S.A. 95 (7): 3461-3466 (1998). PMID 9520388.

            2022.05.17

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