Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-BOS-EX

Mammalian expression vector with human EF-1a.

Sequence (full)	RDB07940zzk03.seq provided by the depositor
Remarks, protocol and/or map (pdf)	RDB07940.pdf
Remarks, protocol and/or map (png)	RDB07940_map.png

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって、長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB07940_B1Ifp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F Region: SV40 pro_ori, EF1alpha pro Sequence file: RDB07940_B1Ifa.seq
>RDB07940_B1If_1_lacIpro-B_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNAGTGAGCG AGGNAGCGGA AGANCGCCCA    61 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG   121 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT   181 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC   241 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT TAATTCCCTC   301 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC   361 CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG   421 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA   481 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTT GCAAAGATGG   541 ATAAAGTTTT AAACAGAGAG GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA   601 GTGGGAATTG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCNNNN NNTCCCCGAG   661 AAGTTGGGGG GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC   721 TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTNNNNA NGGTGGGGGA NAANCGTATA   781 TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC ANAACACAGG   841 TAAGTGNCGT GTGTGGNNTN CCCGCNGGCC TGGCCTCTTT ACGGGTNANG GCCCTTGCGN   901 GCCNTNGANT TACNTNCNNC CTNNNNTGCA NNACGTGATT CNTNGNNTNN NNNANCTTNN   961 NGGGTTNGGA AGTGGGTGGG NNNANNTTCN ANGNNCNTNN CNCNTNANNN NNCNNCCNTN 1021 NGCCNCNNNC NTNGNANNNN NNNGNNNNNG NNNNNNGNNN NNNNNNN //
Primer: f1ori_R3 Region: hGCSF 3'UTR + pA Sequence file: RDB07940_B1Ifb.seq
>07940_B1If_1_f1ori_R3_E04_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCC GCACAGATGC GTAAGGAGAA AATACCGCAT    61 CAGGCGCCAT TCNNNNNNNA NGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC   121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC   181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAAATTC GGTTTTTTTT   241 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 301 TTTTTTTTTT TTTNNNNNNN NNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, EF1alpha pro Sequence file: RDB07940_B1Ifc.seq
>07940_B1If_1_SV40pro_ori_F_H06_24_ABI24.ab1     1 NNNNNNNNNN NNNNNGNCGC CTCGGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT    61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT TGCAAAGATG GATAAAGTTT TAAACAGAGA   121 GGAATCTTTG CAGCTAATGG ACCTTCTAGG TCTTGAAAGG AGTGGGAATT GGCTCCGGTG   181 CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG   241 GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT   301 ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG   361 TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT   421 CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACCT   481 GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA   541 GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT   601 GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT   661 CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC   721 TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG   781 ACNGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC   841 CGANAATCGG ANGGNNGTAG TCTCAAGCTG GCCGGNCNGC TCTGGTGCCN GGCCTCNCGC   901 CGCCNNGTAT CGCCCCGCCC TNNNNGCAAN GCTGGNCCNG NCNGNANCAG TTNCNNGANC 961 NNAAAGNNNG NCNCTNNCNN NNCCNNNNNN NNNN //
Primer: hGCSF_3UTR_F1 Region: hGCSF 3'UTR + pA, MCS, EF1alpha pro Sequence file: RDB07940_B1Ifd.seq
>07940_B1If_1_hGCSF_3UTR_F1_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTCCTC CTNNCTGCTC CNTCCNNCNT CCCNNGTCCC    61 CGTCCNGCAA GGCNNNNNNN NNNNNNNNNN NCTGGNCCTA AAAAGAGTCA CAGTCNNNNN   121 NNNNNNGGGG GCAGGGCAGG TGTCCCGAGG CCACCCAGAA AAACAGGAGA CACGTCGGTG   181 ATGTTCGGGA GTCAAACCAT GTCCCAAAAG TCTTAAGAAG AAAAACGAGA TTCCCCAGCA   241 AATTCGTGGG ACCCCAGGGC CATGCCTCCC AGCTCTGGCC ACCTCTGCTT GTCCAGGGGC   301 CTCACAGCCA AGGGGATGCA GGGGCCGCTG TGCAGTCGGC TGAGGCCAGA GTGAGGGGTG   361 CAAGGACCCA GATGGGGAAC ACTGCTGTTT AAATATTAAA CAGGGATTTC TTGTCTCCCA   421 CGTGGGAGAC CTGATACTCT CAAGGGTCCC AGGTGGGGAC CCTCACTCTA GAGTCGACGC   481 TAGCGGATCC CCGGGCGGCC GCGGTACCGA ATTCCTCACG ACACCTGAAA TGGAAGAAAA   541 AAACTTTGAA CCACTGTCTG AGGCTTGAGA ATGAACCAAG ATCCAAACTC AAAAAGGGCA   601 AATTCCAAGG AGAATTACAT CAAGTGCCNA GCTGGCCTAA CTTCAGTCTC CACCCNCTCA   661 GTGTNNNGAA ACTCCATCGN ATAAANNCCC TCCCCCCNAC CTAAAGANNA NNTACTNNNA 721 ANNCTCNNNN NNNNNN //
Primer: EF1a-C_F Region: hGCSF 3'UTR + pA, MCS, EF1alpha pro Sequence file: RDB07940_B1Ife.seq
>RDB07940_B1If_1_EF1a-C_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT NNNNNNNNNT GGNNNNNNGN NNNNNCTCNN NNCTCNNNNN    61 NTGGTTCAAA GTTTTTTTCT TCCATTTCNN GTGTCGTGAG GAATTCGGTA CCGCGGCCGC   121 CCGGGGATCC GCTAGCGTCG ACTCTAGAGT GAGGGTCCCC ACCTGGGACC CTTGAGAGTA   181 TCAGGTCTCC CACGTGGGAG ACAAGAAATC CCTGTTTAAT ATTTAAACAG CAGTGTTCCC   241 CATCTGGGTC CTTGCACCCC TCACTCTGGC CTCAGCCGAC TGCACAGCGG CCCCTGCATC   301 CCCTTGGCTG TGAGGCCCCT GGACAAGCAG AGGTGGCCAG AGCTGGGAGG CATGGCCCTG   361 GGGTCCCACG AATTTGCTGG GGAATCTCGT TTTTCTTCTT AAGACTTTTG GGACATGGTT   421 TGACTCCCGA ACATCACCGA CGTGTCTCCT GTTTTTCTGG GTGGCCTCGG GACACCTGCC   481 CTGCCCCCAC GAGGGTCAGG ACTGTGACTC TTNNNNNNGC CAGGCAGGTG CCTGGACATT   541 TGCCTTGCTG GACGGGGACT GGGGATGTGG GAGGGAGCAG ACAGGAGGAA TCATGTCAGG   601 CCTGTGTGTG AAAGGAAGCT CCACTGTCAC CCTCCACCTC TTCACCCCCC ACTCACCAGT   661 GTCCCCTCCA CTGTCACATT GTAACTGAAC TTCAGGATAA TAAAGTGTTT GCCTCCNNNN 721 NNNAANNAAA NNNNNAANNA NNNNNNNNNA NNNNNNNNNN //
Primer: AmpR_F Region: AmpR 3', ori Sequence file: RDB07940_B1Iff.seq
>RDB07940_B1If_1_AmpR_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCG CTGNNNNAGG TGCCTCACTG ATTAAGCATT    61 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT   121 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC   181 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG   241 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG   301 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA   361 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA   421 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA   481 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC   541 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA   601 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA   661 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC   721 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC   781 GTCGATTTTT GTGATGCTCG TCNNNNGGNC GGANCCTATG GAAAAACGCC AGCAACGCGG   841 CCTTTTTACG GTTCCTGNNC TTTTGCTGGC CTTTTGCTCA CATGTNCTTT CCTGCGTTAT   901 CCCCNGATTC TGTGGATAAC CGTATNNNCG CCTTTGANTG AGCTGATNCC GCTCGNCNGC   961 ANCNNANNNA NCNANCGCAN CGNNNNAGTG ANCNANNNNN CNNANNNNCG CCNNANNNNN 1021 NANNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.25

GNP_filter3_RDBDEP_html_220812.pl