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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-BOS

Mammalian expression vector with human EF-1a.

Catalog number RDB07939
Resource name pEF-BOS
Clone info. Mammalian expression vector with human EF-1a.. Related vector pEF-BOS (RDB07939) is also available.
Comment Please refer Prof. Nagata's web page for datails (http://biochemi.ifrec.osaka-u.ac.jp/english/research/pef-bos.html).
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5,685 bp
Selectable markers Amp^r
Depositor|Developer Nagata, Shigekazu |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって、長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB07939 pEF-BOS DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB07939z.seq
            Remarks, protocol and/or map (pdf) RDB07939.pdf
            Remarks, protocol and/or map (png) RDB07939_map.png

            Original reference

            original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB07939_A6Cmp1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F
            Region: EF-1 alpha pro, hG-CSF pA
            Sequence file: RDB07939_A6Cma.seq check
            >07939_07939_A6Cm_EF1a-C_F_E03_15.ab1
                1 ACCCATGGGA ATTGGCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT
               61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA ATTCTCTAGA GATCCCTCGA
              121 CCTCGAGATC CATTGTGCTG GCGCGGATTC TTTATCACTG ATAAGTTGGT GGACATATTA
              181 TGTTTATCAG TGATAAAGTG TCAAGCATGA CAAAGTTGCA GCCGAATACA GTGATCCGTG
              241 CCGCCCTGGA CCTGTTGAAC GAGGTCGGCG TAGACGGTCT GACGACACGC AAACTGGCGG
              301 AACGGTTGGG GGTTCAGCAG CCGGCGCTTT ACTGGCACTT CAGGAACAAG CGGGCGCTGC
              361 TCGACGCACT GGCCGAAGCC ATGCTGGCGG AGAATCATAC GCATTCGGTG CCGAGAGCCG
              421 ACGACGACTG GCGCTCATTT CTGATCGGGA ATGCCCGCAG CTTCAGGCAG GCGCTGCTCG
              481 CCTACCGCCA GCACAATGGA TCTCGAGGGA TCTTCCATAC CTACCAGTTC TGCGCCTGCA
              541 GGTCGCGGCC GCGACTCTAG AGTGAGGGTC CCCACCTGGG ACCCTTGAGA GTATCAGGTC
              601 TCCCACGTGG GAGACAAGAA ATCCCTGTTT AATATTTAAA CAGCAGTGTT CCCCATCTGG
              661 GTCCTTGCAC CCCTCACTCT GGCCTCAGCC GACTGCACAG CGGCCCCTGC ATCCCCTTGG
              721 CTGTGAGGCC CCTGGACAAG CAGAGGTGGC CAGAGCTGGG AGGCATGGCC CTGGGGTCCC
              781 ACGAATTTGC TGGGGAATCT CGTTTTTCTT CTTAAGACTT TTGGGACATG GTTTGACTCC
              841 CGAACATCAC CGACGTGTCT CCTGTTTTTC TGGGTGGCCT CGGGACACCT GCCCTGCCCC
              901 CACGAGGGTC AGGACTGTGA CTCTTTTTAG GGCCAGGCAG GTGCCTGGAC ATTTGCCTTG
              961 CTGGACGGGA CTGGGGGATG TGGGGAGGGA GCAGACAGGA AGGAATCATG TCAGCCCTGT
             1021 GTGTGAAAGG AAGCTCCACT GGTCACCCCT CACCTCTTCA CCCCCCCACT CAACAAGGTG
             1081 TCCCCTCCAC TGTCACATTG GTACTGAACT CAGATTATTA AGTGTTTGGC CCTCCCAAAG
            1141 AAGGAGATGA TGATGTGAAT G
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283.
            reference Wakabayashi-Ito, N., Characterization of the regulatory elements in the promoter of the human elongation factor-1 alpha gene. J. Biol. Chem. 269 (47): 29831-29837 (1994). PMID 7961976.
            reference Uetsuki, T., Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1 alpha. J. Biol. Chem. 264 (10): 5791-5798 (1989). PMID 2564392.

            2022.05.17

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