Sequencing and PCR primers for primer information.
Mammalian expression vector with human EF-1a.
| Catalog number | RDB07939 |
|---|---|
| Resource name | pEF-BOS |
| Clone info. | Mammalian expression vector with human EF-1a.. Related vector pEF-BOS (RDB07939) is also available. Please refer Prof. Nagata's web page for datails (http://biochemi.ifrec.osaka-u.ac.jp/english/research/pef-bos.html). |
| Vector backbone | pEF-BOS (Plasmid) |
| Size of vector backbone | 5,685 bp |
| Selectable markers | Amp^r |
| Depositor | Nagata, Shigekazu | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB07939 | pEF-BOS | DNA solution |
Electronic file
| Electronic file | Sequence RDB07939z.seq from Depositor |
|---|---|
| Electronic file | Remarks, protocol and/or map (pdf) RDB07939.pdf from Depositor |
Original reference
| original | Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Empty Backbone (English text) |
|---|---|
| Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB07939_A6Cmp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: EF1a-C_F | Sequence: RDB07939_A6Cma.seq |
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>07939_07939_A6Cm_EF1a-C_F_E03_15.ab1 1 ACCCATGGGA ATTGGCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT 61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA ATTCTCTAGA GATCCCTCGA 121 CCTCGAGATC CATTGTGCTG GCGCGGATTC TTTATCACTG ATAAGTTGGT GGACATATTA 181 TGTTTATCAG TGATAAAGTG TCAAGCATGA CAAAGTTGCA GCCGAATACA GTGATCCGTG 241 CCGCCCTGGA CCTGTTGAAC GAGGTCGGCG TAGACGGTCT GACGACACGC AAACTGGCGG 301 AACGGTTGGG GGTTCAGCAG CCGGCGCTTT ACTGGCACTT CAGGAACAAG CGGGCGCTGC 361 TCGACGCACT GGCCGAAGCC ATGCTGGCGG AGAATCATAC GCATTCGGTG CCGAGAGCCG 421 ACGACGACTG GCGCTCATTT CTGATCGGGA ATGCCCGCAG CTTCAGGCAG GCGCTGCTCG 481 CCTACCGCCA GCACAATGGA TCTCGAGGGA TCTTCCATAC CTACCAGTTC TGCGCCTGCA 541 GGTCGCGGCC GCGACTCTAG AGTGAGGGTC CCCACCTGGG ACCCTTGAGA GTATCAGGTC 601 TCCCACGTGG GAGACAAGAA ATCCCTGTTT AATATTTAAA CAGCAGTGTT CCCCATCTGG 661 GTCCTTGCAC CCCTCACTCT GGCCTCAGCC GACTGCACAG CGGCCCCTGC ATCCCCTTGG 721 CTGTGAGGCC CCTGGACAAG CAGAGGTGGC CAGAGCTGGG AGGCATGGCC CTGGGGTCCC 781 ACGAATTTGC TGGGGAATCT CGTTTTTCTT CTTAAGACTT TTGGGACATG GTTTGACTCC 841 CGAACATCAC CGACGTGTCT CCTGTTTTTC TGGGTGGCCT CGGGACACCT GCCCTGCCCC 901 CACGAGGGTC AGGACTGTGA CTCTTTTTAG GGCCAGGCAG GTGCCTGGAC ATTTGCCTTG 961 CTGGACGGGA CTGGGGGATG TGGGGAGGGA GCAGACAGGA AGGAATCATG TCAGCCCTGT 1021 GTGTGAAAGG AAGCTCCACT GGTCACCCCT CACCTCTTCA CCCCCCCACT CAACAAGGTG 1081 TCCCCTCCAC TGTCACATTG GTACTGAACT CAGATTATTA AGTGTTTGGC CCTCCCAAAG 1141 AAGGAGATGA TGATGTGAAT G // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283. |
|---|
2019.11.02
GNP_filter3_RDBDEP_html_190927.pl