Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-BOS

Mammalian expression vector with human EF-1a.

Sequence (full)	RDB07939zzk03.seq provided by the depositor
Remarks, protocol and/or map (pdf)	RDB07939.pdf
Remarks, protocol and/or map (png)	RDB07939_map.png

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって、長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB07939_A6Cmp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro, hG-CSF pA Sequence file: RDB07939_A6Cma.seq

>07939_07939_A6Cm_EF1a-C_F_E03_15.ab1     1 ACCCATGGGA ATTGGCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT    61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA ATTCTCTAGA GATCCCTCGA   121 CCTCGAGATC CATTGTGCTG GCGCGGATTC TTTATCACTG ATAAGTTGGT GGACATATTA   181 TGTTTATCAG TGATAAAGTG TCAAGCATGA CAAAGTTGCA GCCGAATACA GTGATCCGTG   241 CCGCCCTGGA CCTGTTGAAC GAGGTCGGCG TAGACGGTCT GACGACACGC AAACTGGCGG   301 AACGGTTGGG GGTTCAGCAG CCGGCGCTTT ACTGGCACTT CAGGAACAAG CGGGCGCTGC   361 TCGACGCACT GGCCGAAGCC ATGCTGGCGG AGAATCATAC GCATTCGGTG CCGAGAGCCG   421 ACGACGACTG GCGCTCATTT CTGATCGGGA ATGCCCGCAG CTTCAGGCAG GCGCTGCTCG   481 CCTACCGCCA GCACAATGGA TCTCGAGGGA TCTTCCATAC CTACCAGTTC TGCGCCTGCA   541 GGTCGCGGCC GCGACTCTAG AGTGAGGGTC CCCACCTGGG ACCCTTGAGA GTATCAGGTC   601 TCCCACGTGG GAGACAAGAA ATCCCTGTTT AATATTTAAA CAGCAGTGTT CCCCATCTGG   661 GTCCTTGCAC CCCTCACTCT GGCCTCAGCC GACTGCACAG CGGCCCCTGC ATCCCCTTGG   721 CTGTGAGGCC CCTGGACAAG CAGAGGTGGC CAGAGCTGGG AGGCATGGCC CTGGGGTCCC   781 ACGAATTTGC TGGGGAATCT CGTTTTTCTT CTTAAGACTT TTGGGACATG GTTTGACTCC   841 CGAACATCAC CGACGTGTCT CCTGTTTTTC TGGGTGGCCT CGGGACACCT GCCCTGCCCC   901 CACGAGGGTC AGGACTGTGA CTCTTTTTAG GGCCAGGCAG GTGCCTGGAC ATTTGCCTTG   961 CTGGACGGGA CTGGGGGATG TGGGGAGGGA GCAGACAGGA AGGAATCATG TCAGCCCTGT  1021 GTGTGAAAGG AAGCTCCACT GGTCACCCCT CACCTCTTCA CCCCCCCACT CAACAAGGTG  1081 TCCCCTCCAC TGTCACATTG GTACTGAACT CAGATTATTA AGTGTTTGGC CCTCCCAAAG 1141 AAGGAGATGA TGATGTGAAT G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

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