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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-BOS

Mammalian expression vector with human EF-1a.

Catalog number RDB07939
Resource name pEF-BOS
Clone info. Mammalian expression vector with human EF-1a.. Related vector pEF-BOS (RDB07939) is also available.
Comment Please refer Prof. Nagata's web page for datails (http://biochemi.ifrec.osaka-u.ac.jp/english/research/pef-bos.html).
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5,685 bp
Selectable markers Amp^r
Depositor|Developer Nagata, Shigekazu |
Other clones in our bank

External Database

            Reference sequence
              
             
            Sequence (full) RDB07939zzk03.seq provided by the depositor
            Remarks, protocol and/or map (pdf) RDB07939.pdf
            Remarks, protocol and/or map (png) RDB07939_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって、長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB07939 pEF-BOS DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Mammalian Expression Vector, pEF-BOS series (English text)
            Featured content 哺乳動物細胞用の遺伝子発現ベクター pEF-BOSシリーズ (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB07939_B1L9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ColE1ori_F
            Region: ori, SV40 pro_ori
            Sequence file: RDB07939_B1L9a.seq check
            >0939A2_B1L9_2_ColE1ori_F_A03_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCN NNGNNTTGAN CGTCGATTTT TGTGATGCTC
               61 GTCAGGGGGG CGGNNCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
              121 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
              181 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
              241 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
              301 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
              361 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
              421 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
              481 CTATGACCAT GATTACGCCA AGCTTAATTC CCTCCCCAGC AGGCAGAAGT ATGCAAAGCA
              541 TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA
              601 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGANT AATTTTTTTT ATTTATGCNG
              661 AGGNCGANGN CGCCTCNGCC TCTGANCTAT TNNNGAAGTA NTGAGGANGN TTTTTTGNNN
            721 GNCNNGGNNT TNNNNAAAAG NNNN
            //
            Primer: M13ori_R1
            Region: M13 ori, hGCSF 3'UTR + pA
            Sequence file: RDB07939_B1L9b.seq check
            >D07939A2_B1L9_2_M13ori_R1_E01_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NNGNAGGGNA NAAAGCGAAA GGAGCGGGCG
               61 CTAAGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA
              121 ATGCGCCGCT ACAGGGCGCG TACTATGGTT GCTTTGACGT ATGCGGTGTG AAATACCGCA
              181 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG
              241 TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG
              301 TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
              361 GACGGCCAGT GAATTCGGTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
            421 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTNNNNNNNN NN
            //
            Primer: SV40pro_ori_F
            Region: SV40 ori_pro, EF1 alpha pro
            Sequence file: RDB07939_B1L9c.seq check
            >07939_B1L9_2_SV40pro_ori_F_H03_24_ABI24.ab1
                1 NNNNNNNNNN NNNNGCCGCC TCGGCCTCTG ANCTATTCNN GAAGTAGTGA GGAGGCTTTT
               61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTTT GCAAAGATGG ATAAAGTTTT AAACAGAGAG
              121 GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA GTGGGAATTG GCTCCGGTGC
              181 CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG
              241 CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA
              301 CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT
              361 GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG GTAAGTGCCG TGTGTGGTTC
              421 CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG CCTTGAATTA CTTCCACCTG
              481 GCTGCAGTAC GTGATTCTTG ATCCCGAGCT TCGGGTTGGA AGTGGGTGGG AGAGTTCGAG
              541 GCCTTGCGCT TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT GAGGCCTGGC CTGGGCGCTG
              601 GGGCCGCCGC GTGCGAATCT GGTGGCACCT TCGCGCCTGT CTCGCTGCTT TCGATAAGTC
              661 TCTAGCCATT TAAAATTTTT GATGACCTGC TGCGACGCTT TTTTTCTGGC AAGATAGTCT
              721 TGTAAATGCG GGCCAAGATC TGCACACTGG TATTTCGGTT TTTGGGGCCG CGGGCGGCGA
              781 CGGGGCCCGT GCGTCCCAGC GCACATGTTC GGCGAGGCGG GGCCTGCGAN CGCGGCCACC
              841 GAGAATCGGA CGGGGGTANT CTCAAGCTGG CCGGNCTGCT CTGGTGCCNN GCCTCGCGCC
              901 GCCNNGNATC GCCCCGCCCN NNNNGGNAAG GCTGGNCCNG NNNGCACCAN TTGCNNNANC
            961 NNAAAGANGG NNNNTTCCNN NNNNNNNN
            //
            Primer: EF1a-C_F
            Region: EF1 alpha pro
            Sequence file: RDB07939_B1L9d.seq check
            >0939A2_B1L9_2_EF1a-C_F_Beta_A05_02_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTTTT TGANTTTGGN NCTTGGTTNN TTCNNNNNNC TCAGACAGTG
               61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTAGAG ATCCCTCGAC
              121 CTCGAGATCC ATTGTGCTGG CGCGGATTCT TTATCACTGA TAAGTTGGTG GACATATTAT
              181 GTTTATCAGT GATAAAGTGT CAAGCATGAC AAAGTTGCAG CCGAATACAG TGATCCGTGC
              241 CGCCCTGGAC CTGTTGAACG AGGTCGGCGT AGACGGTCTG ACGACACGCA AACTGGCGGA
              301 ACGGTTGGGG GTTCAGCAGC CGGCGCTTTA CTGGCACTTC AGGAACAAGC GGGCGCTGCT
              361 CGACGCACTG GCCGAAGCCA TGCTGGCGGA GAATCATACG CATTCGGTGC CGAGAGCCGA
              421 CGACGACTGG CGCTCATTTC TGATCGGGAA TGCCCGCAGC TTCAGGCAGG CGCTGCTCGC
              481 CTACCGCCAG CACAATGGAT CTCGAGGGAT CTTCCATACC TACCAGTTCT GCGCCTGCAG
              541 GTCGCGGCCG CGACTCTAGA GTGAGGGTCC CCACCTGGGN NCCTTGAGAG TATCAGGTCT
              601 CCCACGTGGG AGACAAGAAA TCCCTGTTTA ATATTTAAAC NGCAGTGNTC CCCATCTGGG
              661 TCCTTGCNCC CCTCNNTCNN GNNTCNNNNN ANNGNNCANN NGNNCCCNNN NNNCCNNNNN
            721 NNNNN
            //
            Primer: EF1apro_up_F1
            Region: hGCSF 3'UTR + pA
            Sequence file: RDB07939_B1L9e.seq check
            >D07939A2_B1L9_2_EF1apro_up_F1_Beta_B02_05_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNNN NNNNNCGACG CACTGGCCGA ANNCCNTGCT
               61 GGCGGANAAT CATACGCATT CGGTGCCGAG AGCCGACGAC GACTGGCGCT CATTTCTGAT
              121 CGGGAATGCC CGCAGCTTCA GGCAGGCGCT GCTCGCCTAC CGCCAGCACA ATGGATCTCG
              181 AGGGATCTTC CATACCTACC AGTTCTGCGC CTGCAGGTCG CGGCCGCGAC TCTAGAGTGA
              241 GGGTCCCCAC CTGGGACCCT TGAGAGTATC AGGTCTCCCA CGTGGGAGAC AAGAAATCCC
              301 TGTTTAATAT TTAAACAGCA GTGTTCCCCA TCTGGGTCCT TGCACCCCTC ACTCTGGCCT
              361 CAGCCGACTG CACAGCGGCC CCTGCATCCC CTTGGCTGTG AGGCCCCTGG ACAAGCAGAG
              421 GTGGCCAGAG CTGGGAGGCA TGGCCCTGGG GTCCCACGAA TTTGCTGGGG AATCTCGTTT
              481 TTCTTCTTAA GACTTTTGGG ACATGGTTTG ACTCCCGAAC ATCACCGACG TGTCTCCTGT
              541 TTTTCTGGGT GGCCTCGGGA CACCTGCCCT GCCCCCACGA GGGTCAGGAC TGTGACTCTT
              601 TTTAGGGCCA GGCAGGTGCC TGGACATTTG CCTTGCTGGA NGGGGANTGG GGGANGNGGG
              661 ANGGANCANA CAGGANGANT CNTGTCNNNN TGTGTGNGNA ANNNAANCTN CNCTGTCNNC
            721 CTNNNNNCNN NNCCCCCNNN CNNNNNNNNC CNNNNNNNNN N
            //
            Primer: tetpro-B_R
            Region: M13 ori
            Sequence file: RDB07939_B1L9f.seq check
            >0939A2_B1L9_2_tetpro-B_R_Beta_F05_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNCNNNNNNN TGNNGCTCCC GGNNNNGGTC ACAGCTTGTC
               61 TGTAAGCGGA TGNNNNNNNN NNNNNNNNCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT
              121 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATAAAA
              181 TTGTAAACGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT
              241 TTAACCAATA GACCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG CCCGAGATAG
              301 AGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG
              361 TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCAAAT
              421 CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC
              481 GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA
              541 AAGGAGCGGG CGCTAAGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC
              601 CCGCCGCGCT TAATGCGCCG CTACNGGGCG CGTACTATGG TTGCTTTGAC GTATGCGGTG
              661 TGAAATANCG CACAGATGCG TAANGAGAAA ATACCGCATC NNNNGCNNTT CNNNNTTCNN
            721 NNGCNNNNCN NNNNGGNNNG NNNNNNGNNN NNGNNNNNNN NNN
            //
            Primer: f1oriF3
            Region: M13 ori, AmpR pro, AmpR 5'
            Sequence file: RDB07939_B1L9g.seq check
            >0939A2_B1L9_2_f1oriF3_Beta_D05_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTTN NGCCCTTNGN NGTNGGAGTC CNCGTTCTTT
               61 AATAGTGGAC TCTTGTTCCA NANNGNNNNN NCACTCAACT CTATCTCGGG CTATTCTTTT
              121 GATTTATAAG GGATTTTGCC GATTTCGGTC TATTGGTTAA AAAATGAGCT GATTTAACAA
              181 AAATTTAACG CGAATTTTAA CAAAATATTA ACGTTTACAA TTTTATGGTG CACTCTCAGT
              241 ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC
              301 GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC
              361 GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC
              421 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA
              481 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT
              541 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA
              601 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCANTT
              661 TGCCTTCCTG NTTTTNNTCN CCCNGAAACG CTGGNGAAAN TAAAANNNGC NNNNNNCANN
            721 NGGNNNNNNN NNN
            //
            Primer: AmpR_F
            Region: ori
            Sequence file: RDB07939_B1L9h.seq check
            >07939_B1L9_2_AmpR_F_A02_02_ABI24
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC TGNNANAGGT GCCTCACTGN ATTAAANCAT
               61 TGGTAACTGT CNNACCNNNT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT
              121 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA
              181 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA
              241 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG
              301 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC
              361 AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG
              421 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC
              481 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG
              541 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC
              601 NCCGAACTGA GATACCTACA GCGTGANCTA TGAGAAAGCG CCACGCTTCC CGAANGGNAG
              661 AAAGGNGGAC AGGTNTCCNG NNANCGGNNG GGNNNNANNN NGANANCGNN NNNNGNNNCT
            721 NNNNNGGGGN AAANNNNNNG NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283.
            reference Wakabayashi-Ito, N., Characterization of the regulatory elements in the promoter of the human elongation factor-1 alpha gene. J. Biol. Chem. 269 (47): 29831-29837 (1994). PMID 7961976.
            reference Uetsuki, T., Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1 alpha. J. Biol. Chem. 264 (10): 5791-5798 (1989). PMID 2564392.

            2022.08.25

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