Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-BOS

Mammalian expression vector with human EF-1a.

Catalog number RDB07939
Resource name pEF-BOS
Clone info. Mammalian expression vector with human EF-1a.. Related vector pEF-BOS (RDB07939) is also available.
Please refer Prof. Nagata's web page for datails (http://biochemi.ifrec.osaka-u.ac.jp/english/research/pef-bos.html).
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5,685 bp
Selectable markers Amp^r
Depositor|Developer Nagata, Shigekazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07939 pEF-BOS DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB07939z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB07939.pdf

Original reference

original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07939_A6Cmp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Region: EF-1 alpha pro, hG-CSF pA
Sequence file: RDB07939_A6Cma.seq check
>07939_07939_A6Cm_EF1a-C_F_E03_15.ab1
    1 ACCCATGGGA ATTGGCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT
   61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA ATTCTCTAGA GATCCCTCGA
  121 CCTCGAGATC CATTGTGCTG GCGCGGATTC TTTATCACTG ATAAGTTGGT GGACATATTA
  181 TGTTTATCAG TGATAAAGTG TCAAGCATGA CAAAGTTGCA GCCGAATACA GTGATCCGTG
  241 CCGCCCTGGA CCTGTTGAAC GAGGTCGGCG TAGACGGTCT GACGACACGC AAACTGGCGG
  301 AACGGTTGGG GGTTCAGCAG CCGGCGCTTT ACTGGCACTT CAGGAACAAG CGGGCGCTGC
  361 TCGACGCACT GGCCGAAGCC ATGCTGGCGG AGAATCATAC GCATTCGGTG CCGAGAGCCG
  421 ACGACGACTG GCGCTCATTT CTGATCGGGA ATGCCCGCAG CTTCAGGCAG GCGCTGCTCG
  481 CCTACCGCCA GCACAATGGA TCTCGAGGGA TCTTCCATAC CTACCAGTTC TGCGCCTGCA
  541 GGTCGCGGCC GCGACTCTAG AGTGAGGGTC CCCACCTGGG ACCCTTGAGA GTATCAGGTC
  601 TCCCACGTGG GAGACAAGAA ATCCCTGTTT AATATTTAAA CAGCAGTGTT CCCCATCTGG
  661 GTCCTTGCAC CCCTCACTCT GGCCTCAGCC GACTGCACAG CGGCCCCTGC ATCCCCTTGG
  721 CTGTGAGGCC CCTGGACAAG CAGAGGTGGC CAGAGCTGGG AGGCATGGCC CTGGGGTCCC
  781 ACGAATTTGC TGGGGAATCT CGTTTTTCTT CTTAAGACTT TTGGGACATG GTTTGACTCC
  841 CGAACATCAC CGACGTGTCT CCTGTTTTTC TGGGTGGCCT CGGGACACCT GCCCTGCCCC
  901 CACGAGGGTC AGGACTGTGA CTCTTTTTAG GGCCAGGCAG GTGCCTGGAC ATTTGCCTTG
  961 CTGGACGGGA CTGGGGGATG TGGGGAGGGA GCAGACAGGA AGGAATCATG TCAGCCCTGT
 1021 GTGTGAAAGG AAGCTCCACT GGTCACCCCT CACCTCTTCA CCCCCCCACT CAACAAGGTG
 1081 TCCCCTCCAC TGTCACATTG GTACTGAACT CAGATTATTA AGTGTTTGGC CCTCCCAAAG
1141 AAGGAGATGA TGATGTGAAT G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283.

2020.10.02

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