Resource data sheet
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pEF-BOS (#RDB07939)

Mammalian expression vector with human EF-1a.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Mizushima, S., Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Mammalian expression vector with human EF-1a.. Related vector pEF-BOS (RDB07939) is also available.
Comment Please refer Prof. Nagata's web page for datails (http://biochemi.ifrec.osaka-u.ac.jp/english/research/pef-bos.html).
Vector backbone pEF-BOS (RDB07939) (Plasmid)
Size of vector backbone 5,685 bp
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Depositor|Developer Nagata, Shigekazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって、長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee
RDB07939 pEF-BOS DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pEF-BOS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07939).

Reference section:

Mizushima, S., Nagata, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283. [PubMed] [Article] [RRC of NBRP]
reference Wakabayashi-Ito, N., Characterization of the regulatory elements in the promoter of the human elongation factor-1 alpha gene. J. Biol. Chem. 269 (47): 29831-29837 (1994). PMID 7961976. [PubMed] [RRC of NBRP]
reference Uetsuki, T., Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1 alpha. J. Biol. Chem. 264 (10): 5791-5798 (1989). PMID 2564392. [PubMed]