Resource data sheet
Prev. |  Lentiviral vector plasmid > 
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-CMV-MCS-IRES2-mRFP1

Backbone vector of lentivirus construct driven by CMV promoter with mRFP1 protein marker.

Catalog number RDB07918
Resource name CSII-CMV-MCS-IRES2-mRFP1
Clone info. Backbone vector of lentivirus construct driven by CMV promoter with mRFP1 protein marker.
XbaI site at 4490nt is not exist. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS ()
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction. Please contact us. UCSD's MTA is also required.
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07918 CSII-CMV-MCS-IRES2-mRFP1 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB07918z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB07918.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12751_A4D4p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_F
Region: CMV en 2,CMV pro 2,IRES2
Sequence file: RDB12751_A4D4g.seq check
>07918_12751_A4D4_cPPT+CTS_F_G04_19.ab1
    1 AAATGGATTA TTCGATTATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG
   61 GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
  121 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT
  181 TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT
  241 CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT
  301 GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC
  361 GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
  421 TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA
  481 AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA AATGGGCGGT
  541 AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTGGTT TAGTGAACCG TCAGATCCGC
  601 TAGCGCTACC GGTCTCGAGA ATTCTGCAGC GGCCGCGGAT CCGCCCCTCT CCCTCCCCCC
  661 CCCCTAACGT TACTGGCCGA AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT
  721 TATTTTCCAC CATATTGCCG TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT
  781 TCTTGACGAG CATTCCTAGG GGTCTTTCCC CTCTCGCCAA AGGAATGCAA AGTCTGTTGA
  841 ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG CTTCTTGAAG ACAACAACGT CTGTAGCGAC
  901 CCTTTGCAAG GCAGCGGACC CCCACCTGGC GACAGTGCCT CTGCGGCCAA AAGCCACGTG
  961 TATAGATACA CCCTGCAAGG CGCACAACCC CAGTGCACGT TGTGAGTTGG ATAGTGTGGA
 1021 GAGTCCAATG GCTCCTCCTC AAGCGTATCA CCAGGGCTGA GGATGCCAGA AGTACCCATG
1081 TATGGTATCG ATCGAGCTCG GTACCATGCC TTTACATGTG ATTAGTCGAG GTTTA
//
Primer: WPRE_R
Region: mRFP1 3'
Sequence file: RDB12751_A4D4h.seq check
>07918_12751_A9LqF_WPRE_R_H01_08_ABI08.ab1
    1 NNNNNNNNNN TNNNNNNNNN TTTNNAATCN NGAGGTTGAN TATCGATAAG CTTGATCCCT
   61 CGATGTTAAC TCTAGTCGAG TGCGGCCGCT TAGGCGCCGG TGGAGTGGCG GCCCTCGGCG
  121 CGCTCGTACT GTTCCACGAT GGTGTAGTCC TCGTTGTGGG AGGTGATGTC CAGCTTGATG
  181 TCGGTCTTGT AGGCGCCGGG CAGCTGCACG GGCTTCTTGG CCATGTAGGT GGTCTTGACC
  241 TCGGCGTCGT AGTGGCCGCC GTCCTTCAGC TTCAGCCTCA TCTTGATCTC GCCCTTCAGG
  301 GCGCCGTCCT CGGGGTACAT CCGCTCGGTG GAGGCCTCCC AGCCCATGGT CTTCTTCTGC
  361 ATTACGGGGC CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT TCACCTTGTA GATGAACTCG
  421 CCGTCCTGCA GGGAGGAGTC CTGGGTCACG GTCACCACGC CGCCGTCCTC GAAGTTCATC
  481 ACGCGCTCCC ACTTGAAGCC CTCGGGGAAG GACAGCTTCA AGTAGTCGGG GATGTCGGCG
  541 GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC TGGAACTGAG GGGACAGGAT GTCCCAGGCG
  601 AAGGGCAGGG GGCCGCCCTT GGTCACCTTC AGCTTGGCGG TCTGGGTGCC CTCGTAGGGG
  661 CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT TCACGGAGCC CTCCATGCGC
  721 ACCTTGAAGC GCATGAACTC CTTGATGACG TCCTCGGANG ANGCCATANG CCCGTGGCCA
  781 TATTATCATC GTGTTTTTCN AAGGAAAACC ACGTCNCCGT GGTTCGGGGG GCCTAGACGN
  841 TTTTTTAACC TCGACTAAAC ACATGTAAAG CATGTGTACC GAGGNCCCNN ATCAGATNCN
  901 ATACNANGGG GGTANCTTCT GGNNATCCTT CNNCCCNTTN TTGAATACGC TNNANGANAN
  961 CNNTTNNANT NNTNNNNNNA CNNTCNNNCT CNNNNCGNNN NNNNGGGGGN TNNNNNNNNC
 1021 NNTNNNNNNN NNNNNNTNNN NNNNNNNGNN NTTNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNGNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNN
//
Primer: HIVgp_R1
Region: HIV-1 psi,5'LTR,CMV pro 1
Sequence file: RDB12751_A4D4i.seq check
>07918_12751_A4D4_HIVgp_R1_A11_02.ab1
    1 CCCGGCCTCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
  181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
  241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
  301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
  361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
  421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
  481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
  541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
  601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
  661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT
  721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT
  781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG
  841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA
  901 CACGCCTACC GCCCATTTGC GTCAATGGGG GCGGAGTTGT TACGACATTT TGGAAAGTCC
  961 CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT
 1021 CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAACCGC ATCACCATGG
 1081 TAATAGCGAT GACTAATACG TAGATGTACT GCCAGTAGAA AGTCCCATAA GTCATGTACC
 1141 TGGCCATATG GCAGCCGGCA TTACGTCATG AACGTCAATA GGGGGGGCGC TAACTTGTGG
1201 CCAATATG
//
Primer: IRES-F
Region: IRES2,mRFP1 5'
Sequence file: RDB12751_A4D4j.seq check
>07918_12751_A9LqF_IRES-F_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTNNG TCGANGNNNN NNAAACGTCT AGGCCCCCCG AACCNCGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CGGGCCTATG GCCTCCTCCG
  121 AGGACGTCAT CAAGGAGTTC ATGCGCTTCA AGGTGCGCAT GGAGGGCTCC GTGAACGGCC
  181 ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA
  241 AGCTGAAGGT GACCAAGGGC GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT
  301 TCCAGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC
  361 TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG
  421 TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC
  481 GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG
  541 CCTCCACCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGATGAGGC
  601 TGAAGCTGAA GGACGGCGGC CACTACGACG CCGAGGTCAA GACCACCTAC ATGGCCAAGA
  661 AGCCCGTGCA GCTGCCCGGC GCCTACAAGA CCGACATCAA GCTGGACATC ACCTCCCACA
  721 ACGAGGACTA CACCATCGTG GAACAGTACG AGCGCGCCGA NGGCCGCCAC TCCACCGGCG
  781 CCTAAGCGNN CGCACTCGAC TAGAGTTAAC ATCGAGGGAN CAAGCTTATC GATAATCAAC
  841 CTCTGGATTA CAAAATTTGT GAAAGANTGA CTGGNATTCT TAACTATGTT GCTCCTTTTA
  901 CNCTATGNGG ATACNCNGCT TTAATGNCNT NGNATNNNGC TATTGCTNCC NNNATGGCTT
  961 TCNTTTTCNN NNNCTTGNAT NAANNCNGNN NNNTGNNNNN TTNTGANNAN NNGTGNNCNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN
//
Primer: BGH_rev2
Region: bGHp(A),3'LTR,PRE
Sequence file: RDB12751_A4D4k.seq check
>07918_12751_A4D4_BGHrev_E02_14.ab1
    1 GCATACGGGT CGTGCTAGAG ATTTTGCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
   61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC
  241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA
  301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA
  361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG
  421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT
  481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC
  541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA
  601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG
  661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG
  721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
  781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
  841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG
  901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC
  961 AGCGGGCAAG CAGCGGCGAT GAGTTCCGCC GTGGCAATAG GGAGGGGAAA GCGAAAGTCC
 1021 CGGAAAGAGC TGACAGGTGT GGCATGCCCC ACCAGTGGGG GTTGCGTCAG CAAACACAGT
 1081 GCACACCACG CCACGGTGCT GACAACGGAC AGACTCTCAT AAGAGAACAG CAACAGGAAT
1141 TTATACAGAA GGAAGAAAAA TGGAAAGG
//
Primer: SV40pro_F
Region: SV40 pro_ori,Zeo,SV40p(A)
Sequence file: RDB12751_A4D4l.seq check
>07918_12751_A4D4_SV40pro_F_D11_11.ab1
    1 GCCCGGCTCC CCGTGACCTT TTCAGAAGTA GTGAAGGAGG CTTTTTTGGA GGCCTAGGCT
   61 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA
  121 CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC
  181 CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT
  241 CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG
  301 TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA
  361 CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA
  421 GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA
  481 GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC
  541 CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG
  601 GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT
  661 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAAAT GGTTACAAAT
  721 AAAGCAATAG CATCACAAAT TTCACAAATA AAAGCATTTT TTTCACTGCA TTCTAGTTGT
  781 GGTTTGTCCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA
  841 GAGCTTGGCG TAAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA
  901 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA
  961 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
 1021 GCCAAGCTGC ATTTATTGAT TCGGCCAACG CCCCGGGGAG AGGCCGGTTT GCGTATTGGG
 1081 GCGCTTCTTC GGCTTCCTCG CCTCCACTGA CCTCGCCTGC GCTCGGTCGT TCGCCTGCCG
 1141 GCGAGCGGTA TTCAGCCCTC ACCTCAAGGC GTATACGGTT ATCCCACTGG AATCAGGGGA
1201 TATACGCCAG GAGACCTGTG ACCAATAGGC TCCAGCTCTA TAAGGACG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.01.07

GNP_filter3_RDBDEP_html_191130.pl