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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-CMV-MCS-IRES2-mRFP1

Backbone vector plasmid of lentivirus construct driven by CMV promoter with mRFP1 protein marker.

Catalog number RDB07918
Resource name CSII-CMV-MCS-IRES2-mRFP1
Clone info. Backbone vector plasmid of lentivirus construct driven by CMV promoter with mRFP1 protein marker.
XbaI site at 4490nt is not exist. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS ()
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction.[link] UCSD's MTA is also required. Please visit Additional Forms section.
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
UCSD's MTA is also required. Please visit Additional Forms section.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07918 CSII-CMV-MCS-IRES2-mRFP1 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB07918z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB07918.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17905_B0Aep1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_F
Region: CMV en 2,CMV pro 2,IRES2
Sequence file: RDB17905_B0Aea.seq check
>07918_17905_B0Ae_1_cPPT+CTS_F_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNTNGNNNA NTAGTANTCN ATTACGGGGT CATTAGTTCN TAGCCCATAT
   61 ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC
  121 CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC
  181 CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG
  241 TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
  301 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
  361 ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT
  421 GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC
  481 CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC
  541 GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTG GTTTAGTGAA CCGTCAGATC
  601 CGCTAGCGCT ACCGGTCTCG AGAATTCTGC AGCGGCCGCG GATCCGCCCC TCTCCCTCCC
  661 CCCCCCCTAA CGTTACTGGC CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA
  721 TGTTATTTTC CACCATATTG CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCTGGCCCTG
  781 TCTTCTTGAC GAGCATTCCT AGGGGTCTTT CNNCTCTCGC CNAAGGAATG CAAGGTCTGT
  841 TGAATGTCGT GAANGAAGCA GTTCCTCTGG AAGCTTCTTG AAGANAAACA ACGTCTGTAN
  901 CGANCCTTTG CAGGCAGCGG AANCCCCCAC CNNGNCGANA GGNGCCNNNG CGGNCNAAAG
  961 CCNCGNGNAT NANANACNCC TGCAAANGNG NNACNNCCCN NNNNNCNNNN NGTGANTNNG
1021 NNANNNGNGN NNNANTNNAN NNNNNNNNN
//
Primer: IRES-F
Region: IRES2,mRFP1
Sequence file: RDB17905_B0Aeb.seq check
>07918_17905_B0Ae_1_IRES-F_E11_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTNN GTCGANGTNN NNNNNNCGTC TAGGCCCCCC GAACCACGGG
   61 GNCGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACGGGCCTAT GGCCTCCTCC
  121 GAGGACGTCA TCAAGGAGTT CATGCGCTTC AAGGTGCGCA TGGAGGGCTC CGTGAACGGC
  181 CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC
  241 AAGCTGAAGG TGACCAAGGG CGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG
  301 TTCCAGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG
  361 CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG
  421 GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG
  481 CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG
  541 GCCTCCACCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGATGAGG
  601 CTGAAGCTGA AGGACGGCGG CCACTACGAC GCCGAGGTCA AGACCACCTA CATGGCCAAG
  661 AAGCCCGTGC AGCTGCCCGG CGCCTACAAG ACCGACATCA AGCTGGACAT CACCTCCCAC
  721 AACGAGGACT ACACCATCGT GGAACAGTAC GAGCGCGCCG ANGGCCGCCA CTCCACCGGC
  781 GCCTAAGCGG NCGCACTCGA CTAGAGTTAA CATCGAGGGA TCAAGCTTAT CGATAATCNA
  841 CCTCTGGATT ACNAAATTTG TGAAAGANTG ACTGGTATTC TTAACTATGT TGCTCCTTTT
  901 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CNGTATGGNT
  961 TTCNTTTTCT CCNNCTNGNA TAAATCCNNN NTNNNTGNCT CTTTNTGAGG ANNNNNNGNN
1021 CNNNNGNCNN NNNNCGNNNN NNNNNN
//
Primer: PRE_rev
Region: PRE,mRFP1
Sequence file: RDB17905_B0Aec.seq check
>05552_18578_B0Kq_PRE_rev_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCNNCNTA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGNN TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGAGGCT
  121 CGAGAGGCCT TGAATTCTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG
  181 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG
  241 GACTGGTAGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG
  301 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CCGCCGTCCT CGATGTTGTG GCGGATCTTG
  361 AAGTTGGCCT TGATGCCGTT CTTCTGCTTG TCGGCGGTGA TATAGACGTT GTGGCTGTTG
  421 TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC
  481 AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG
  541 TTGCCGTCGT CCTTGAAGAA GANGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC
  601 TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTG CANGCCGTAN
  661 CCNANGGTGG TCACGANGGT GGGNCAGGGN NCGGGNAGCT NGNCNNNNGN GNANNNNNNN
721 NNN
//
Primer: HIV-B_R
Region: HIV-1 psi,5' LTR,CMV pro 1,CMV en 1
Sequence file: RDB17905_B0Aed.seq check
>07918_17905_B0Ae_1_HIV-B_R_C11_08_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNNNN AANNTTTTGG CGTACTCNCC AGTCGCCGCC CCTCGCCTCT
   61 TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT
  121 CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT
  181 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT
  241 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG
  301 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC
  361 CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT
  421 TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG
  481 ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG
  541 CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA
  601 TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG
  661 GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA
  721 CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT
  781 TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG
  841 TTATGTAACG CGGAACTCCA TATATGGGCT ATGAACTAAT GANCCCGTAA TTGATTACTA
  901 TTAATAACTA GTCAATAATC AATGTCAACG CGNATATCTG GNCCGTANNT CGCGAANCAN
  961 CGNAAAANNC CTAACCCTAA NCANANTCTT CNTGNNATTG NCGGNNNNGC CTNNNCNNNN
1021 NNGNANCTNN AATTTNGNNC NNNNNNNNNN
//
Primer: cPPT/CTS-E_R
Region: cPPT_CTS,RRE
Sequence file: RDB17905_B0Aee.seq check
>07918_17905_B0Ae_1_cPPT_CTS-E_R_D11_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTG NATTCTTNAG TTTGTATGTC TGTTGCTATT ATGTCTACTA
   61 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA
  121 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT
  181 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC
  241 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT
  301 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT
  361 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA
  421 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG
  481 TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA
  541 TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGAGCAACCC
  601 CAAATCCCCA GGAGCTGTTG ATCCTTTAGG TATCTTTCCA CAGCCAGGAT TCTTGCCTGG
  661 AGCTGCTTGA TGCCCCAGAC TGTGAGTTGC AACAGATGCT GTTGCGCCTC AATAGCCCTC
  721 AGCAAATTGT TCTGCTGCTG CACTATACCA GACAATAATT GTCTGGCCTG TACCGTCAGC
  781 GTCATTGACG CTGCGCCCAT AGTGCTTCCT GCTGCTCCCA AGAACCCAAG GAACAAAGCT
  841 CCTATTCCCA CTGCTCTTTT TTCTCTCTGC ACCACTCTTC TCTTTGCNNT GGTGGGTGCT
  901 ACTCCNAATG GNTCNATTTT NACNANTTNA TATTTATATA ATTCACTTCT NNNATTGNCC
  961 NNCANATCTC CTNCNNCNNG NCTNAANNNC NNCGGNCGGN NGCTTGNTGN GNNNNGNNNN
1021 TANNTTTGNT TNNNNNNNNN N
//
Primer: BGH_rev2
Region: bGHpA,3' LTR,PRE
Sequence file: RDB17905_B0Aef.seq check
>07918_17905_B0Ae_1_BGH_rev2_F11_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGGGGNNGG GNAACAACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
  121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
  181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
  241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG
  301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG
  361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC
  421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG
  481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG
  541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
  601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
  661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
  721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
  781 TCGACGGTAT CGATGCGGGG ANGCGGNCCA AAGGGAGATC CGACTCGTCT GANGGCGAAN
  841 GCGAAGANGC GGAAGANGCC GCANANCCGG CAGCAGGCCG CGGGAANGAA NGNCCGCTGG
  901 NNTGNNGGNC GAANGGNCGT ANCANAANGA NGTCCNGCGC ANANTCCAGG NGGNNACACA
  961 GGCNANCANC CNNNGNAAGG NNGTCAGCTT NCCNNANNNC ANNNCNGNAN NNGTCANNGN
1021 CNNNNANCCG ANNNNNNNNN N
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,Zeo,SV40 pA
Sequence file: RDB17905_B0Aeg.seq check
>07918_17905_B0Ae_1_SV40pro_ori_F_G11_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN CCTCTGCCTC TGANCTATTC CNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC
  181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC
  241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG
  301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG
  361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG
  421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG
  481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC
  541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC
  601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC
  661 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT
  721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
  781 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG
  841 TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
  901 TATCCGCTCA CAATTCCNCA CAACATACGA NNNGNANNCN TAAAGTGNAA AGCCNGGGGT
  961 GNCTAANGAN NGAGCTAANN CACANNAATT GNNTTGCNNN NNCNGNCCGC TTTCCANNCN
1021 GGNAANCNNN CNNGNCNANC NNCNTNNNNG NANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Kakizawa, S., Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells. Sci. Rep. 10 (1): 14475 (2020). PMID 32879382.

2020.12.30

GNP_filter3_RDBDEP_html_201222.pl