Resource data sheet
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CSII-CMV-MCS-IRES2-mRFP1

Backbone vector of lentivirus construct driven by CMV promoter with mRFP1 protein marker.

Catalog number RDB07918
Resource name CSII-CMV-MCS-IRES2-mRFP1
Clone info. Backbone vector of lentivirus construct driven by CMV promoter with mRFP1 protein marker.
XbaI site at 4490nt is not exist. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS ()
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. Please contact us. UCSD's MTA is also required.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
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RDB07918 CSII-CMV-MCS-IRES2-mRFP1 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07918z.seq from Depositor
Electronic file PDF RDB07918.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12751_A4D4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_F RDB12751_A4D4a_v2.seq
>07918_12751_A4D4_cPPT+CTS_F_G04_19.ab1
    1 AAATGGATTA TTCGATTATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG
   61 GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
  121 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT
  181 TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT
  241 CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT
  301 GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC
  361 GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
  421 TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA
  481 AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA AATGGGCGGT
  541 AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTGGTT TAGTGAACCG TCAGATCCGC
  601 TAGCGCTACC GGTCTCGAGA ATTCTGCAGC GGCCGCGGAT CCGCCCCTCT CCCTCCCCCC
  661 CCCCTAACGT TACTGGCCGA AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT
  721 TATTTTCCAC CATATTGCCG TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT
  781 TCTTGACGAG CATTCCTAGG GGTCTTTCCC CTCTCGCCAA AGGAATGCAA AGTCTGTTGA
  841 ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG CTTCTTGAAG ACAACAACGT CTGTAGCGAC
  901 CCTTTGCAAG GCAGCGGACC CCCACCTGGC GACAGTGCCT CTGCGGCCAA AAGCCACGTG
  961 TATAGATACA CCCTGCAAGG CGCACAACCC CAGTGCACGT TGTGAGTTGG ATAGTGTGGA
 1021 GAGTCCAATG GCTCCTCCTC AAGCGTATCA CCAGGGCTGA GGATGCCAGA AGTACCCATG
1081 TATGGTATCG ATCGAGCTCG GTACCATGCC TTTACATGTG ATTAGTCGAG GTTTA
//
Primer: IRES-F RDB12751_A4D4b_v2.seq
>07918_12751_A4D4_IRES-F_A09_03.ab1
    1 CTGCCTTTTA AGGTTGGTTT TAGTCGAGGG TTAAAAAAAC GTCCTAGGCC CCCCCGAAAC
   61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACGG GCCTATGGCC
  121 TCCTCCGAGG ACGTCATCAA GGAGTTCATG CGCTTCAAGG TGCGCATGGA GGGCTCCGTG
  181 AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCACCCAG
  241 ACCGCCAAGC TGAAGGTGAC CAAGGGCGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC
  301 CCTCAGTTCC AGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT CCCCGACTAC
  361 TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT CGAGGACGGC
  421 GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT CTACAAGGTG
  481 AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC
  541 TGGGAGGCCT CCACCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAA
  601 GATGAGGCTG AAGCTGAAGG ACGGCGGCCA CTACGACGCC GAGGTCAAGA CCACCTAACA
  661 TGGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAAGA CCGACATCAA GCTGGACATC
  721 ACCTCCCACA ACGAGGACTA CACCCATCGT GGAACAGTAC GAGCGCGGCC GAGGGGCCGC
  781 CACTCCACCT GGCGCCCTAA GCGGCCGCAC TTCGAATAAA AGTTAACATC CGAAGGGAAT
841 C
//
Primer: HIVgp_R1 RDB12751_A4D4c_v2.seq
>07918_12751_A4D4_HIVgp_R1_A11_02.ab1
    1 CCCGGCCTCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
  181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
  241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
  301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
  361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
  421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
  481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
  541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
  601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
  661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT
  721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT
  781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG
  841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA
  901 CACGCCTACC GCCCATTTGC GTCAATGGGG GCGGAGTTGT TACGACATTT TGGAAAGTCC
  961 CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT
 1021 CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAACCGC ATCACCATGG
 1081 TAATAGCGAT GACTAATACG TAGATGTACT GCCAGTAGAA AGTCCCATAA GTCATGTACC
 1141 TGGCCATATG GCAGCCGGCA TTACGTCATG AACGTCAATA GGGGGGGCGC TAACTTGTGG
1201 CCAATATG
//
Primer: BGH_rev2 RDB12751_A4D4d_v2.seq
>07918_12751_A4D4_BGHrev_E02_14.ab1
    1 GCATACGGGT CGTGCTAGAG ATTTTGCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
   61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC
  241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA
  301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA
  361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG
  421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT
  481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC
  541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA
  601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG
  661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG
  721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
  781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
  841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG
  901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC
  961 AGCGGGCAAG CAGCGGCGAT GAGTTCCGCC GTGGCAATAG GGAGGGGAAA GCGAAAGTCC
 1021 CGGAAAGAGC TGACAGGTGT GGCATGCCCC ACCAGTGGGG GTTGCGTCAG CAAACACAGT
 1081 GCACACCACG CCACGGTGCT GACAACGGAC AGACTCTCAT AAGAGAACAG CAACAGGAAT
1141 TTATACAGAA GGAAGAAAAA TGGAAAGG
//
Primer: SV40pro_F RDB12751_A4D4e_v2.seq
>07918_12751_A4D4_SV40pro_F_D11_11.ab1
    1 GCCCGGCTCC CCGTGACCTT TTCAGAAGTA GTGAAGGAGG CTTTTTTGGA GGCCTAGGCT
   61 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA
  121 CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC
  181 CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT
  241 CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG
  301 TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA
  361 CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA
  421 GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA
  481 GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC
  541 CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG
  601 GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT
  661 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAAAT GGTTACAAAT
  721 AAAGCAATAG CATCACAAAT TTCACAAATA AAAGCATTTT TTTCACTGCA TTCTAGTTGT
  781 GGTTTGTCCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA
  841 GAGCTTGGCG TAAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA
  901 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA
  961 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
 1021 GCCAAGCTGC ATTTATTGAT TCGGCCAACG CCCCGGGGAG AGGCCGGTTT GCGTATTGGG
 1081 GCGCTTCTTC GGCTTCCTCG CCTCCACTGA CCTCGCCTGC GCTCGGTCGT TCGCCTGCCG
 1141 GCGAGCGGTA TTCAGCCCTC ACCTCAAGGC GTATACGGTT ATCCCACTGG AATCAGGGGA
1201 TATACGCCAG GAGACCTGTG ACCAATAGGC TCCAGCTCTA TAAGGACG
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.10.09

GNP_filter3_RDBDEP_html_180823.pl