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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CS-RfA-ETBsd

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Bsd marker driven by EF-1 alpha promoter.

Catalog number RDB07917
Resource name CS-RfA-ETBsd
Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Bsd marker driven by EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-RfA-ETBsd ()
Selectable markers Lentivirus transfection: Bsd^r. E. coli: Amp^r, Cm^r, ccdB. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction.[link] 
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Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07917 CS-RfA-ETBsd DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB07917z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB07917.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12129_A2H6p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R
Region: CmR,attR1,cPPT_CTS
Sequence file: RDB12129_A2H6a.seq check
>07917_12129_A9JoF_Cm_M_R_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NTATNNCCNG CTNNNCGTCT TTCNTTGCCN TACGGAATTC
   61 CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT
  121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT
  181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC
  241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT
  301 CGACGGATCC TAACTCAAAA TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC
  361 TGGGGTGCCT AATGCGGCCG CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA
  421 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT
  481 CGTTCAGCTT TTTTGTACAA ACTTGTGATA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA
  541 TAAACCCGAA AATTTTGAAT TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT
  601 GTTGCTATTA TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT
  661 TTAAAATTGT GGATGAATAC TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC
  721 TCTGTCTCTC TCTCCACCTT CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT
  781 TGGGANGTGG GTCTGAAACG ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA
  841 AAGTACAGCA AAAACTATTC TTAAACCTAN NNAGCCTCCN ANTATCATTA TGAATAATTT
  901 TATATACCAN AGCCNATTTG TTATGTTAAA CNNATTNNNC NAACTTGCCN ATTTATCTAA
  961 NNNCNNNANT TCNNNNNCNT TCNTTTTNNN NNNNNNTTNN NNANNNNTNN NNNTNNANNN
1021 NNNNNNN
//
Primer: EF1a-N_R
Region: EF1alpha pro,attR2,ccdB
Sequence file: RDB12129_A2H6b.seq check
>07917_12129_A9JoF_EF1a-N_R_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNGNNNNNNN GGCGNNGTGC GCTCTGCCCN CTGNCGGGCA CCGGNNCCTC
   61 ACGAAGCTTG AATTATCACC ACTTTGTACA AGAAAGCTGA ACGAGAAACG TAAAATGATA
  121 TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA
  181 CACAACATAT CCAGTCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC
  241 ATTTATATTC CCCAGAACAT CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG
  301 AGATCAGCCA CTTCTTCCCC GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC
  361 ATGCGCCAGC TTTCATCCCC GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT
  421 GACAGCAGAC GTGCACTGGC CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA
  481 TCACTCTGTA CATCCACAAA CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA
  541 AACTGCATTT CACCAGCCCC TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG
  601 GGCGACCTCA GCCATCCCTT CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG
  661 GGCTTCATTC TGCATGGTTG TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG
  721 AGCAACTGAT AGCTGTCGCT GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC
  781 TTTTTGACAT ACTTCGGGTA TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC
  841 GCAAATACGC ATACTGTTAT CTGGCTTTTA NTAAGCCGGA TCCACGCGTT TACGCCCCGC
  901 CCNGCCANTC ATCGCANNAN TGTTGTAATT CATTAAGCNT TCTGCCGANN NGGNANGCCN
961 TCACNAANGG NNNGANGNNN CNNGNNNCGN CNNCNNNNNN NNNN
//
Primer: PRE_rev
Region: PRE,BSD,T2A,TetR
Sequence file: RDB12129_A2H6c.seq check
>07917_12129_A9JoF_PRE_rev_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGNNCANC ATAGTTAAGA ATACCAGTCA ATCTTTCACA
   61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGTGC GGCCTTAGCC
  121 CTCCCACACA TAACCAGAGG GCAGCAATTC ACGAATCCCA ACTGCCGTCG GCTGTCCATC
  181 ACTGTCCTTC ACTATGGCTT TGATCCCAGG ATGCAGATCG AGAAGCACCT GTCGGCACCG
  241 TCCGCAGGGG CTCAAGATGC CCCTGTTCTC ATTTCCGATC GCGACGATAC AAGTCAGGTT
  301 GCCAGCTGCC GCAGCAGCAG CAGTGCCCAG CACCACGAGT TCTGCACAAG GTCCCCCAGT
  361 AAAATGATAT ACATTGACAC CAGTGAAGAT GCGGCCGTCG CTAGAGAGAG CTGCGCTGGC
  421 GACGCTGTAG TCTTCAGAGA TGGGGATGCT GTTGATTGTA GCCGTTGCTC TTTCAATGAG
  481 GGTGGATTCT TCTTGAGACA AAGGCTTGGC CATGGGCCCG GGATTTTCCT CCACGTCACC
  541 GCATGTTAGA AGACTTCCTC TGCCCTCGCC GGATCCGCTG TACGCGGACC CACTTTCACA
  601 TTTAAGTTGT TTTTCTAATC CGCATATGAT CAATTCAAGG CCGAATAAGA AGGCTGGCTC
  661 TGCACCTTGG TGATCAAATA ATTCGATAGC TTGTCGTAAT AATGGCGGCA TACTATCAGT
  721 AGTAGGTGTT TCCCTTTCTT CTTTAGCGAC TTGATGCTCT TGATCTTCCA ATACGCAACC
  781 TAAAGTAAAA TGCCCCACAG CGCTGANTGC ATATAATGCA TTCTCTANTG AAAAACCTTG
  841 TTGGCATAAA AAGGCTAATT GATTTTCGAG ANTTTCATAC NGTTTTTCTG TANNCCGNGT
  901 ANCNNAANGT ACTTTTNGCT NCATCGCGAT NNNNTANTAA AGCACATCTA AAACTTTTNA
  961 NCNNTANTAC NNNAAAAANT CNTNGNCNNN NNTNNNNNNN TNNNNANNGN NNAANNNNNN
1021 NNNN
//
Primer: CMV_Forward
Region: CMV pro,5'LTR,HIV-1 psi
Sequence file: RDB12129_A2H6d.seq check
>07917_12129_A9JoF_CMV_Forward_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGANCAC CGCACAGCAA GCGGCCGCTG
  781 ATCTTCAGAN CTGGANGANG AGATATGANG GANAATTGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAATTGAANN NTTANGNNTA GCACCNACCA AGGNAAANAN AANANNGGTG
  901 CNNANAGAAA AAAAGAGCAG NGGGAATAGG ANCTTNGTNN CTNNGGTNNT NNGGNNCANC
961 NNNAANCNCT NNNGNNNNNN NNNN
//
Primer: RRE-M_R
Region: RRE
Sequence file: RDB12129_A2H6e.seq check
>07917_12129_A9JoF_RRE-M_R_B05_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT AGCCNNNNNN NNNNNGTTCT
   61 GCTGCTGCAC TATACCAGAC AATAATTGTC TGGCCTGTAC CGTCAGCGTC ATTGACGCTG
  121 CGCCCATAGT GCTTCCTGCT GCTCCCAAGA ACCCAAGGAA CAAAGCTCCT ATTCCCACTG
  181 CTCTTTTTTC TCTCTGCACC ACTCTTCTCT TTGCCTTGGT GGGTGCTACT CCTAATGGTT
  241 CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA ATTGTCCCTC ATATCTCCTC
  301 CTCCAGGTCT GAAGATCAGC GGCCGCTTGC TGTGCGGTGG TCTTACTTTT GTTTTGCTCT
  361 TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT CTATCCTTTG ATGCACACAA
  421 TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT CTGATCCTGT CTGAAGGGAT
  481 GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG TTTCTAACAG GCCAGGATTA
  541 ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT GTTTTAATTT ATATTTTTTC
  601 TTTCCCNNNN NNCTTAACCG AATTTTTTCC CATCGCGATC TAATTCTCCC CCGCTTAATA
  661 NNNNNNCTCT CGCACNNNNN NCTCTCCTTC TAGCCTCCGC TANTCNAAAT TTTTGGCGTA
  721 CTCNNNNNNC NCCGCCCCNT CNNNNCTNGN CGTGCGCGCT TCNNNNNNCN AGTCCTGCNT
  781 CNANAGAGCT NCTCTGGTTT NCCNTNTCNN TTTCAAGTCC CTGTTCNNGC NCCNNTGCTA
  841 NANANTTTNN ACNCTGACNN AANNGNNNTG ANGGANCTCT ANTTNNCNNN NNNNNNCNNC
901 NNACNGNNNC NNACNNCNTN NAAGCNNTNN NNNNNNNN
//
Primer: BGH_rev2
Region: 3'LTR
Sequence file: RDB12129_A2H6f.seq check
>07917_12129_A9JoF_BGH_rev2_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGGGNNGG GNNNNNNNCA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGNTNNGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
  121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
  301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
  361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
  421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
  481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCNNTN NNGGAAGTAG CCTTGTGTGT
  541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
  601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
  661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
  721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
  781 CTCGACGGTA TCGATGCGGG GANGCGGCCC AAAGGGANAT CCGANTCGTC TGANGGCGAA
  841 NGCGAAGANG CGNAANANGC CGCANANCCG GCAGCANGCC GCGGGAANGA AGGTCCGCTN
  901 GATTGANGGC CGAANNGNNN NNANCAGAAN GGNCNTCCNN CNCANNNTCC NNGNGGNNNC
961 ACNNGNNANC NNNNNNNGNN ANNGNNNNNN NNNN
//
Primer: SV40pro_F
Region: Zeo
Sequence file: RDB12129_A2H6g.seq check
>07917_12129_A9JoF_SV40pro-F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNANNNNN NNGNNGGNNG CTTTTTTGGA GGCCTAGGCT
   61 TTTGCANANN GCTCCCGGGA GCTTNNNTAT CCATTTTCGG ATCTGATCAG CACGTGTTGA
  121 CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC
  181 CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT
  241 CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG
  301 TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA
  361 CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA
  421 GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA
  481 GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC
  541 CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG
  601 GTTGGGCTTC GGAATCGTTT TCCGGGACGC NNNNNGGATG ATCCTCCAGC GCGGGGATCT
  661 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA
  721 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCNNTGCATT CTAGTTGTGG
  781 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGANCT CTAGCTAGAG
  841 CTTGNCGTAA TCATGGTCAT AGCTGTTTTC CTGTGTGAAA TTGTTATCCG CNCACNATTC
  901 CACACAACAT ACGANCCGGA ANCATAAAGT GNNAANGNCN GGGGGTGCCT AATGAGNGAN
  961 CNNNNTCACA TTANNNGCGT TGCGNNNNNT GNCCNCTTTN NNGNNNNGNN AANNNNNNNN
1021 NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

reference Isaji, T., An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J. Biol. Chem. 289 (30): 20694-20705 (2014). PMID 24895123.
user report Morita, K., Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Cell 181 (3): 702-715.e20 (2020). PMID 32315619.
user report Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219.
user report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
user report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
user report Hikasa, H., Merlin/NF2-Lin28B-let-7 Is a Tumor-Suppressive Pathway that Is Cell-Density Dependent and Hippo Independent. Cell Rep. 14 (12): 2950-2961 (2016). PMID 26997273.
user report Hirokawa, T., CBP-93872 inhibits NBS1-mediated ATR activation, abrogating maintenance of the DNA double-strand break-specific G2 checkpoint. Cancer Res. 74 (14): 3880-3889 (2014). PMID 24876101.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl