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CS-RfA-ETBsd (#RDB07917)

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Bsd marker driven by EF-1 alpha promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Bsd marker driven by EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CS-RfA-ETBsd ()
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli), Bsd (lentivirus transfection). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee
RDB07917 CS-RfA-ETBsd Please contact us. JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CS-RfA-ETBsd was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07917).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

reference Isaji, T., An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J. Biol. Chem. 289 (30): 20694-20705 (2014). PMID 24895123. [PubMed] [Article] [RRC of NBRP]
user_report Watanabe, K., Inhibition of the galactosyltransferase C1GALT1 reduces osteosarcoma cell proliferation by interfering with ERK signaling and cell cycle progression. Cancer Gene Ther. 2024 Apr 15. doi: 10.1038/s41417-024-00773-9. Epub ahead of print. PMID 38622340. [PubMed] [Article] [RRC of NBRP]
user_report Wu, W., Ribosomal S6 kinase (RSK) plays a critical role in DNA damage response via the phosphorylation of histone lysine demethylase KDM4B. Breast Cancer Res. 26 (1): 146 (2024). PMID 39434131. [PubMed] [Article] [RRC of NBRP]
user_report Yang, S., Neutrophil Extracellular Traps Delay Diabetic Wound Healing by Inducing Endothelial-to-Mesenchymal Transition via the Hippo pathway. Int J Biol Sci. 2023 Jan 1;19(1):347-361 (2023). PMID 36594092. [PubMed] [Article] [RRC of NBRP]
user_report Guhathakurta, S., COX17 acetylation via MOF-KANSL complex promotes mitochondrial integrity and function. Nat. Metab. 5 (11): 1931-1952 (2023). PMID 37813994. [PubMed] [Article] [RRC of NBRP]
user_report Matsui, Y., Chlorambucil-conjugated PI-polyamides (Chb-M'), a transcription inhibitor of RUNX family, has an anti-tumor activity against SHH-type medulloblastoma with p53 mutation. Biochem. Biophys. Res. Commun. 620: 150-157 (2022). PMID 35792512. [PubMed] [Article] [RRC of NBRP]
user_report Hattori, E.Y., A RUNX-targeted gene switch-off approach modulates the BIRC5/PIF1-p21 pathway and reduces glioblastoma growth in mice. Commun. Biol. 5 (1): 939 (2022). PMID 36085167. [PubMed] [Article] [RRC of NBRP]
user_report Yamashita, A., Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells. Stem Cells Transl. Med. 10 (1): 115-127 (2021). PMID 32822104. [PubMed] [Article] [RRC of NBRP]
user_report Suzuk,i N., TP53/p53-FBXO22-TFEB controls basal autophagy to govern hormesis. Autophagy 17 (11): 3776-3793 (2021). PMID 33706682. [PubMed] [Article] [RRC of NBRP]
user_report Hanaki, S., UV-induced activation of ATR is mediated by UHRF2. Genes Cells 26 (6): 447-454 (2021). PMID 33848395. [PubMed] [Article] [RRC of NBRP]
user_report Hanaki, S., PP1 regulatory subunit NIPP1 regulates transcription of E2F1 target genes following DNA damage. Cancer Sci. 112 (7): 2739-2752 (2021). PMID 33939241. [PubMed] [Article] [RRC of NBRP]
user_report Masaki, T., Calcineurin regulates the stability and activity of estrogen receptor alpha. Proc. Natl. Acad. Sci. U.S.A. 118 (44): e2114258118 (2021). PMID 34711683. [PubMed] [Article] [RRC of NBRP]
user_report Morita, K., Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Cell 181 (3): 702-715.e20 (2020). PMID 32315619. [PubMed] [Article] [RRC of NBRP]
user_report Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219. [PubMed] [Article] [RRC of NBRP]
user_report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640. [PubMed] [Article] [RRC of NBRP]
user_report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243. [PubMed] [Article] [RRC of NBRP]
user_report Hikasa, H., Merlin/NF2-Lin28B-let-7 Is a Tumor-Suppressive Pathway that Is Cell-Density Dependent and Hippo Independent. Cell Rep. 14 (12): 2950-2961 (2016). PMID 26997273. [PubMed] [Article] [RRC of NBRP]
user_report Johmura, Y., Defective DNA repair increases susceptibility to senescence through extension of Chk1-mediated G2 checkpoint activation. Sci. Rep. 6: 31194 (2016). PMID 27507734. [PubMed] [Article] [RRC of NBRP]
user_report Hirokawa, T., CBP-93872 inhibits NBS1-mediated ATR activation, abrogating maintenance of the DNA double-strand break-specific G2 checkpoint. Cancer Res. 74 (14): 3880-3889 (2014). PMID 24876101. [PubMed] [Article] [RRC of NBRP]