Resource data sheet
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CS-RfA-ETBsd

Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Bsd marker driven by EF-1 alpha promoter.

Catalog number RDB07917
Resource name CS-RfA-ETBsd
Clone info. Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Bsd marker driven by EF-1 alpha promoter.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-RfA-ETBsd ()
Selectable markers Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells); Bsd^r (lentivirus transfection).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
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Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07917 CS-RfA-ETBsd DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07917z.seq from Depositor
Electronic file PDF RDB07917.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12129_A4Lmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R RDB12129_A4Lma_2.seq
>07917_12129_A4Lm_Cm_M_R_D07_10.ab1
    1 GGCTGAAGGG ACCTATCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG
   61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
  121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
  181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
  241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
  301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
  361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
  421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG
  481 TTCAGCTTTT TTGTACAAAC TTGTGATAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA
  541 AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT
  601 TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT
  661 AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC
  721 TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG
  781 GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA
  841 GTACAGCAAA AACTATTCTT AAACCTACCA AGCCTCCTAC TATCATTATG AATAATTTTA
  901 TATACCACAG CCAATTTGTT ATGTTAAACC AATTCCACAA ACTTGCCCAT TTATCTAATT
  961 CCAATAATTC TTGTTCATTC TTTTCTTGCT GGTTTTGCGA TTCTTCAATT AAGGAGTGTA
 1021 TTAAGCTTTG TGTAATTTGT TAAATTTCTC TGTCCCACTC CATCCAGGGT CGTGTGATTT
 1081 CCAAATCTGT TTCCAGAGAT TTATTACTCC AAACCTAGCA TTTCCAAAGG GCACAGCAGT
1141 GCTGCAAATG AGTTTCCAGA GCACCTCCAA TTCCCCTAGG GGAGCCTGTT TTGAAATTC
//
Primer: EF1a-N_R RDB12129_A4Lmb_2.seq
>07917_12129_A4Lm_EF1a-N_R_C07_07.ab1
    1 GAGGTTGAAT CATGGGGGAT GTGGGCGATG TGCGCTCTGC CCACTGACGG GCACCGGAGC
   61 CTCACGAAGC TTGAATTATC ACCACTTTGT ACAAGAAAGC TGAACGAGAA ACGTAAAATG
  121 ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA
  181 AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT AAGGGAGCCT
  241 GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT TTCGCGGTGG
  301 CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT ATCGGTGGTC
  361 ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG GGAGACTTTA
  421 TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG CGTGTCAATA
  481 ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA GGTGTAAACC
  541 TTAAACTGCA TTTCACCAGC CCCTGTTCTC GTCAGCAAAA GAGCCGTTCA TTTCAATAAA
  601 CCGGGCGACC TCAGCCATCC CTTCCTGATT TTCCGCTTTC CAGCGTTCGG CACGCAGACG
  661 ACGGGCTTCA TTCTGCATGG TTGTGCTTAC CAGACCGGAG ATATTGACAT CATATATGCC
  721 TTGAGCAACT GATAGCTGTC GCTGTCAACT GTCACTGTAA TACGCTGCTT CATAGCATAC
  781 CTCTTTTTGA CATACTTCGG GTATACATAT CAGTATATAT TCTTATACCG CAAAAATCAG
  841 CGCGCAAATA CGCATACTGT TATCTGGCTT TTAGTAAGCC GGATCCACGC GTTTACGCCC
  901 CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAAG
  961 CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC
 1021 GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GCCACGTTTT
 1081 AAATCAAAAC TGGTGAACTC ACCCAGGGAT TGGCTGAGAC GAAAACCATA TCTCAATAAC
1141 CCGTTAGAAT TAGCCCAAGT TCACGTAGAC CGCTACAATC CTTTTGC
//
Primer: pAxEF_F1 RDB12129_A4Lmc_2.seq
>07917_12129_A4Lm_pAxEF_F1_A07_01.ab1
    1 CAATTTTTTT TCTTCATTTT AGGGTGTCGG TGAATTAATT AACCGGCCGC ATACCGTGGC
   61 GGTGGCAGCT AGACTAGCGC TTTGGTAGCC ACCATGTCTA GATTAGATAA AAGTAAAGTG
  121 ATTAACAGCG CATTAGAGCT GCTTAATGAG GTCGGAATCG AAGGTTTAAC AACCCGTAAA
  181 CTCGCCCAGA AGCTAGGTGT AGAGCAGCCT ACATTGTATT GGCATGTAAA AAATAAGCGG
  241 GCTTTGCTCG ACGCCTTAGC CATTGAGATG TTAGATAGGC ACCATACTCA CTTTTGCCCT
  301 TTAGAAGGGG AAAGCTGGCA AGATTTTTTA CGTAATAACG CTAAAAGTTT TAGATGTGCT
  361 TTACTAAGTC ATCGCGATGG AGCAAAAGTA CATTTAGGTA CACGGCCTAC AGAAAAACAG
  421 TATGAAACTC TCGAAAATCA ATTAGCCTTT TTATGCCAAC AAGGTTTTTC ACTAGAGAAT
  481 GCATTATATG CACTCAGCGC TGTGGGGCAT TTTACTTTAG GTTGCGTATT GGAAGATCAA
  541 GAGCATCAAG TCGCTAAAGA AGAAAGGGAA ACACCTACTA CTGATAGTAT GCCGCCATTA
  601 TTACGACAAG CTATCGAATT ATTTGATCAC CAAGGTGCAG AGCCAGCCTT CTTATTCGGC
  661 CTTGAATTGA TCATATGCGG ATTAGAAAAA CAACTTAAAT GTGAAAGTGG GTCCGCGTAC
  721 AGCGGATCCG GCGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTGGA GGAAAATCCC
  781 GGGCCCATGG CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT
  841 ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG CCAGCGCAGC TCTCTCTAGC
  901 GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA CTGGGGGGAC CTTGTGCAGA
  961 ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC CACCCTGACT TTGTATCGTC
 1021 GCGATCCGAA ATGGAGAACA GGGGGCCATC TTTGAAGCCC CTTGCGGACG GTGCCCGACA
 1081 GGGTGGCTTC CTCGATTCTT GCATTCCTGG GATCAAGCCC ATTAGTGAAA GGGACCAAGT
 1141 GATGGGACAG CCGACGGCAG CTTTTGGGAT TCTGATTGCC TGTGCCTCTA GTATGTGTGT
1201 GGAAGGGCCC TAAAGTAGGG ATGCTCG
//
Primer: PRE_rev RDB12129_A4Lmd_2.seq
>07917_12129_A4Lm_PRE_rev_E05_14.ab1
    1 ACGATCAAAT AGCGTAAAGG AGCACATAGT TAAGAATACC AGTCAATCTT TCACAAATTT
   61 TGTAATCCAG AGGTTGATTA TCGATAAGCT TGATCCCTCG AGTGCGGCCT TAGCCCTCCC
  121 ACACATAACC AGAGGGCAGC AATTCACGAA TCCCAACTGC CGTCGGCTGT CCATCACTGT
  181 CCTTCACTAT GGCTTTGATC CCAGGATGCA GATCGAGAAG CACCTGTCGG CACCGTCCGC
  241 AGGGGCTCAA GATGCCCCTG TTCTCATTTC CGATCGCGAC GATACAAGTC AGGTTGCCAG
  301 CTGCCGCAGC AGCAGCAGTG CCCAGCACCA CGAGTTCTGC ACAAGGTCCC CCAGTAAAAT
  361 GATATACATT GACACCAGTG AAGATGCGGC CGTCGCTAGA GAGAGCTGCG CTGGCGACGC
  421 TGTAGTCTTC AGAGATGGGG ATGCTGTTGA TTGTAGCCGT TGCTCTTTCA ATGAGGGTGG
  481 ATTCTTCTTG AGACAAAGGC TTGGCCATGG GCCCGGGATT TTCCTCCACG TCACCGCATG
  541 TTAGAAGACT TCCTCTGCCC TCGCCGGATC CGCTGTACGC GGACCCACTT TCACATTTAA
  601 GTTGTTTTTC TAATCCGCAT ATGATCAATT CAAGGCCGAA TAAGAAGGCT GGCTCTGCAC
  661 CTTGGTGATC AAATAATTCG ATAGCTTGTC GTAATAATGG CGGCATACTA TCAGTAGTAG
  721 GTGTTTCCCT TTCTTCTTTA GCGACTTGAT GCTCTTGATC TTCCAATACG CAACCTAAAG
  781 TAAAATGCCC CACAGCGCTG AGTGCATATA ATGCATTCTC TAGTGAAAAA CCTTGTTGGC
  841 ATAAAAAAGG CTAATTGATT TTCGAGAGTT TCATACTGTT TTTTCTGTAG GCCGTGTACC
  901 TAAATGTACT TTTTGCTCCA TCGCGATGAC TTAGTAAAGC ACATCTAAAA CTTTTTAGCG
  961 TTTATTTACG TAAAAAAAAT CTTGCCAGCT TTTCCCCCTT CTAAAGGTCA AAAGTTGAGT
 1021 ATGGTTGCCT ATTCTACCAT CTCAATTGAC TTAGCGTCGA GCTAAGGCCC GCTATTTTTT
1081 ATAACAATGC
//
Primer: CMV-Forward RDB12129_A4Lme_2.seq
>07917_12129_A4Lm_CMV-Forward_B07_04.ab1
    1 AGTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GCGGCCGCTG
  781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG
  901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA
  961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTTATTGTC
 1021 TGGGTATAGT GCAGCAGCAG AAACAAATTT GCTGAGGGCT ATTGAGGCGC AACAGCATCC
 1081 TGTTGCAACC TCACAGTCTG GGGCATCCAA GGCCAGCTTC CAGGCCAGAA TCCTGGCCTG
1141 GTGGAAAAAG GAATATACTT AAAACGA
//
Primer: BGH_rev RDB12129_A4Lmf_2.seq
>07917_12129_A4Lm_BGHrev_F05_17.ab1
    1 GGCATCGGGG CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT
   61 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG
  121 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA
  181 CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC ATGTGATGAA ATGCTAGGCG
  241 GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA TCCATCCCAT GCAGGCTCAC
  301 AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT TCTACCTTCT CTTGCTCAAC
  361 TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT CTGATCCCTG GCCCTGGTGT
  421 GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG ATCCACAGAT CAAGGATATC
  481 TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT
  541 AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT ACCTGAGGTG TGACTGGAAA
  601 ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA TCAGCATTGG TAGCTGCTGT
  661 ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA CGGTATCGAT GCGGGGAGGC
  721 GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGCGAA GACGCGGAAG AGGCCGCAGA
  781 GCCCGGCAGC AGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG ACGTAGCAGA
  841 ATGACGTCCC GCGCAGATCC AGGTGGCAAC ACAGCGAGCA GCATGGAAGA CGTCAGCTTC
  901 CCCGACACAC ACGGATGTCA GTGCCCACAG CGAGCCCTGT CAGCACGGCC AGCAGCGGCG
  961 ATGATTCGGC AGGCAATAGG AGGGAGCGAG TTCCGAGGAC TGACGTGTGC CATTGACTAC
1021 ATGGTATGGC TCAGCAAACC ATGGC
//
Primer: SV40pro_F RDB12129_A4Lmg_2.seq
>07917_12129_A4Lm_SV40proF_E07_13.ab1
    1 CTTTGGTCCT GTGGCCATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG
  841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
  901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
  961 CATTAATTTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG
 1021 CATTAATGAA TCGGGCCAAC GCGCGGGGAG AGGCGGGTTT GCGTATTGAG CGCTCTTCCG
 1081 CTTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGGTTCGG CTGCGGCGAG CGTATCAGCC
 1141 TCCACCTCCA AAGCCGTAAT TACCGGTTAA TCACAGATCC AGGGCATAAC CGCCCCAGCG
1201 GGCAA
//

Please visit Sequencing and PCR primers for primer information.


References

References

reference Isaji, T., An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J. Biol. Chem. 289 (30): 20694-20705 (2014). PMID 24895123.
user report Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219.
user report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
user report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
user report Hikasa, H., Merlin/NF2-Lin28B-let-7 Is a Tumor-Suppressive Pathway that Is Cell-Density Dependent and Hippo Independent. Cell Rep. 14 (12): 2950-2961 (2016). PMID 26997273.

2018.10.22

GNP_filter3_RDBDEP_html_181012.pl