Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Bsd marker driven by EF-1 alpha promoter.
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Other clones in our bank
External Database Reference sequence |
Sequence | RDB07917z.seq |
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Remarks, protocol and/or map (pdf) | RDB07917.pdf |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
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Ordering | Please visit Ordering instruction.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word ![]() |
Remarks | Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
提供条件 | 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 |
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提供依頼 | 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (非営利機関による非営利学術研究用)[Word ![]() 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB07917 | CS-RfA-ETBsd | DNA solution |
Materials & Methods section:
The CS-RfA-ETBsd was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07917). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Lentivirus Vector (English text) |
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Featured content | Lentivirus Vector (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB12129_A2H6p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: Cm_M_R (Pr0012) Region: CmR,attR1,cPPT_CTS Sequence file: RDB12129_A2H6a.seq ![]() |
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>07917_12129_A9JoF_Cm_M_R_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCNN NTATNNCCNG CTNNNCGTCT TTCNTTGCCN TACGGAATTC 61 CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT 121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT 181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC 241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT 301 CGACGGATCC TAACTCAAAA TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC 361 TGGGGTGCCT AATGCGGCCG CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA 421 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT 481 CGTTCAGCTT TTTTGTACAA ACTTGTGATA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA 541 TAAACCCGAA AATTTTGAAT TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT 601 GTTGCTATTA TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT 661 TTAAAATTGT GGATGAATAC TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC 721 TCTGTCTCTC TCTCCACCTT CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT 781 TGGGANGTGG GTCTGAAACG ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA 841 AAGTACAGCA AAAACTATTC TTAAACCTAN NNAGCCTCCN ANTATCATTA TGAATAATTT 901 TATATACCAN AGCCNATTTG TTATGTTAAA CNNATTNNNC NAACTTGCCN ATTTATCTAA 961 NNNCNNNANT TCNNNNNCNT TCNTTTTNNN NNNNNNTTNN NNANNNNTNN NNNTNNANNN 1021 NNNNNNN // |
Primer: EF1a-N_R (Pr0024) Region: EF1alpha pro,attR2,ccdB Sequence file: RDB12129_A2H6b.seq ![]() |
>07917_12129_A9JoF_EF1a-N_R_A01_01_ABI24.ab1 1 NNNNNNNNNN NNGNNNNNNN GGCGNNGTGC GCTCTGCCCN CTGNCGGGCA CCGGNNCCTC 61 ACGAAGCTTG AATTATCACC ACTTTGTACA AGAAAGCTGA ACGAGAAACG TAAAATGATA 121 TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA 181 CACAACATAT CCAGTCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC 241 ATTTATATTC CCCAGAACAT CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG 301 AGATCAGCCA CTTCTTCCCC GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC 361 ATGCGCCAGC TTTCATCCCC GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT 421 GACAGCAGAC GTGCACTGGC CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA 481 TCACTCTGTA CATCCACAAA CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA 541 AACTGCATTT CACCAGCCCC TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG 601 GGCGACCTCA GCCATCCCTT CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG 661 GGCTTCATTC TGCATGGTTG TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG 721 AGCAACTGAT AGCTGTCGCT GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC 781 TTTTTGACAT ACTTCGGGTA TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC 841 GCAAATACGC ATACTGTTAT CTGGCTTTTA NTAAGCCGGA TCCACGCGTT TACGCCCCGC 901 CCNGCCANTC ATCGCANNAN TGTTGTAATT CATTAAGCNT TCTGCCGANN NGGNANGCCN 961 TCACNAANGG NNNGANGNNN CNNGNNNCGN CNNCNNNNNN NNNN // |
Primer: PRE_rev (Pr0250) Region: PRE,BSD,T2A,TetR Sequence file: RDB12129_A2H6c.seq ![]() |
>07917_12129_A9JoF_PRE_rev_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGGNNCANC ATAGTTAAGA ATACCAGTCA ATCTTTCACA 61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGTGC GGCCTTAGCC 121 CTCCCACACA TAACCAGAGG GCAGCAATTC ACGAATCCCA ACTGCCGTCG GCTGTCCATC 181 ACTGTCCTTC ACTATGGCTT TGATCCCAGG ATGCAGATCG AGAAGCACCT GTCGGCACCG 241 TCCGCAGGGG CTCAAGATGC CCCTGTTCTC ATTTCCGATC GCGACGATAC AAGTCAGGTT 301 GCCAGCTGCC GCAGCAGCAG CAGTGCCCAG CACCACGAGT TCTGCACAAG GTCCCCCAGT 361 AAAATGATAT ACATTGACAC CAGTGAAGAT GCGGCCGTCG CTAGAGAGAG CTGCGCTGGC 421 GACGCTGTAG TCTTCAGAGA TGGGGATGCT GTTGATTGTA GCCGTTGCTC TTTCAATGAG 481 GGTGGATTCT TCTTGAGACA AAGGCTTGGC CATGGGCCCG GGATTTTCCT CCACGTCACC 541 GCATGTTAGA AGACTTCCTC TGCCCTCGCC GGATCCGCTG TACGCGGACC CACTTTCACA 601 TTTAAGTTGT TTTTCTAATC CGCATATGAT CAATTCAAGG CCGAATAAGA AGGCTGGCTC 661 TGCACCTTGG TGATCAAATA ATTCGATAGC TTGTCGTAAT AATGGCGGCA TACTATCAGT 721 AGTAGGTGTT TCCCTTTCTT CTTTAGCGAC TTGATGCTCT TGATCTTCCA ATACGCAACC 781 TAAAGTAAAA TGCCCCACAG CGCTGANTGC ATATAATGCA TTCTCTANTG AAAAACCTTG 841 TTGGCATAAA AAGGCTAATT GATTTTCGAG ANTTTCATAC NGTTTTTCTG TANNCCGNGT 901 ANCNNAANGT ACTTTTNGCT NCATCGCGAT NNNNTANTAA AGCACATCTA AAACTTTTNA 961 NCNNTANTAC NNNAAAAANT CNTNGNCNNN NNTNNNNNNN TNNNNANNGN NNAANNNNNN 1021 NNNN // |
Primer: CMV_Forward (Pr0016) Region: CMV pro,5'LTR,HIV-1 psi Sequence file: RDB12129_A2H6d.seq ![]() |
>07917_12129_A9JoF_CMV_Forward_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC 121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA 181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG 241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC 301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG 361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG 421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT 481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC 541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC 601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA 661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT 721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGANCAC CGCACAGCAA GCGGCCGCTG 781 ATCTTCAGAN CTGGANGANG AGATATGANG GANAATTGGA GAAGTGAATT ATATAAATAT 841 AAAGTAGTAA AAATTGAANN NTTANGNNTA GCACCNACCA AGGNAAANAN AANANNGGTG 901 CNNANAGAAA AAAAGAGCAG NGGGAATAGG ANCTTNGTNN CTNNGGTNNT NNGGNNCANC 961 NNNAANCNCT NNNGNNNNNN NNNN // |
Primer: RRE-M_R (Pr0651) Region: RRE Sequence file: RDB12129_A2H6e.seq ![]() |
>07917_12129_A9JoF_RRE-M_R_B05_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT AGCCNNNNNN NNNNNGTTCT 61 GCTGCTGCAC TATACCAGAC AATAATTGTC TGGCCTGTAC CGTCAGCGTC ATTGACGCTG 121 CGCCCATAGT GCTTCCTGCT GCTCCCAAGA ACCCAAGGAA CAAAGCTCCT ATTCCCACTG 181 CTCTTTTTTC TCTCTGCACC ACTCTTCTCT TTGCCTTGGT GGGTGCTACT CCTAATGGTT 241 CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA ATTGTCCCTC ATATCTCCTC 301 CTCCAGGTCT GAAGATCAGC GGCCGCTTGC TGTGCGGTGG TCTTACTTTT GTTTTGCTCT 361 TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT CTATCCTTTG ATGCACACAA 421 TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT CTGATCCTGT CTGAAGGGAT 481 GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG TTTCTAACAG GCCAGGATTA 541 ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT GTTTTAATTT ATATTTTTTC 601 TTTCCCNNNN NNCTTAACCG AATTTTTTCC CATCGCGATC TAATTCTCCC CCGCTTAATA 661 NNNNNNCTCT CGCACNNNNN NCTCTCCTTC TAGCCTCCGC TANTCNAAAT TTTTGGCGTA 721 CTCNNNNNNC NCCGCCCCNT CNNNNCTNGN CGTGCGCGCT TCNNNNNNCN AGTCCTGCNT 781 CNANAGAGCT NCTCTGGTTT NCCNTNTCNN TTTCAAGTCC CTGTTCNNGC NCCNNTGCTA 841 NANANTTTNN ACNCTGACNN AANNGNNNTG ANGGANCTCT ANTTNNCNNN NNNNNNCNNC 901 NNACNGNNNC NNACNNCNTN NAAGCNNTNN NNNNNNNN // |
Primer: BGH_rev2 (Pr0606) Region: 3'LTR Sequence file: RDB12129_A2H6f.seq ![]() |
>07917_12129_A9JoF_BGH_rev2_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGGGGNNGG GNNNNNNNCA GATGGCTGGC AACTAGAAGG 61 CACAGTCGAG GCTGNTNNGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT 121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT 181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA 241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC 301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG 361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG 421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG 481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCNNTN NNGGAAGTAG CCTTGTGTGT 541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC 601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA 661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG 721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT 781 CTCGACGGTA TCGATGCGGG GANGCGGCCC AAAGGGANAT CCGANTCGTC TGANGGCGAA 841 NGCGAAGANG CGNAANANGC CGCANANCCG GCAGCANGCC GCGGGAANGA AGGTCCGCTN 901 GATTGANGGC CGAANNGNNN NNANCAGAAN GGNCNTCCNN CNCANNNTCC NNGNGGNNNC 961 ACNNGNNANC NNNNNNNGNN ANNGNNNNNN NNNN // |
Primer: SV40pro_F (Pr0110) Region: Zeo Sequence file: RDB12129_A2H6g.seq ![]() |
>07917_12129_A9JoF_SV40pro-F_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNANNNNN NNGNNGGNNG CTTTTTTGGA GGCCTAGGCT 61 TTTGCANANN GCTCCCGGGA GCTTNNNTAT CCATTTTCGG ATCTGATCAG CACGTGTTGA 121 CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC 181 CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT 241 CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG 301 TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA 361 CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA 421 GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA 481 GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC 541 CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG 601 GTTGGGCTTC GGAATCGTTT TCCGGGACGC NNNNNGGATG ATCCTCCAGC GCGGGGATCT 661 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA 721 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCNNTGCATT CTAGTTGTGG 781 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGANCT CTAGCTAGAG 841 CTTGNCGTAA TCATGGTCAT AGCTGTTTTC CTGTGTGAAA TTGTTATCCG CNCACNATTC 901 CACACAACAT ACGANCCGGA ANCATAAAGT GNNAANGNCN GGGGGTGCCT AATGAGNGAN 961 CNNNNTCACA TTANNNGCGT TGCGNNNNNT GNCCNCTTTN NNGNNNNGNN AANNNNNNNN 1021 NN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2023.05.22
GNP_filter3_RDBDEP_html_230512.pl