Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-RfA-ETBsd

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Bsd marker driven by EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12129_A2H6p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R (Pr0012) Region: CmR,attR1,cPPT_CTS Sequence file: RDB12129_A2H6a.seq
>07917_12129_A9JoF_Cm_M_R_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCNN NTATNNCCNG CTNNNCGTCT TTCNTTGCCN TACGGAATTC    61 CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT   121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT   181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC   241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT   301 CGACGGATCC TAACTCAAAA TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC   361 TGGGGTGCCT AATGCGGCCG CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA   421 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT   481 CGTTCAGCTT TTTTGTACAA ACTTGTGATA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA   541 TAAACCCGAA AATTTTGAAT TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT   601 GTTGCTATTA TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT   661 TTAAAATTGT GGATGAATAC TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC   721 TCTGTCTCTC TCTCCACCTT CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT   781 TGGGANGTGG GTCTGAAACG ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA   841 AAGTACAGCA AAAACTATTC TTAAACCTAN NNAGCCTCCN ANTATCATTA TGAATAATTT   901 TATATACCAN AGCCNATTTG TTATGTTAAA CNNATTNNNC NAACTTGCCN ATTTATCTAA   961 NNNCNNNANT TCNNNNNCNT TCNTTTTNNN NNNNNNTTNN NNANNNNTNN NNNTNNANNN 1021 NNNNNNN //
Primer: EF1a-N_R (Pr0024) Region: EF1alpha pro,attR2,ccdB Sequence file: RDB12129_A2H6b.seq
>07917_12129_A9JoF_EF1a-N_R_A01_01_ABI24.ab1     1 NNNNNNNNNN NNGNNNNNNN GGCGNNGTGC GCTCTGCCCN CTGNCGGGCA CCGGNNCCTC    61 ACGAAGCTTG AATTATCACC ACTTTGTACA AGAAAGCTGA ACGAGAAACG TAAAATGATA   121 TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA   181 CACAACATAT CCAGTCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC   241 ATTTATATTC CCCAGAACAT CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG   301 AGATCAGCCA CTTCTTCCCC GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC   361 ATGCGCCAGC TTTCATCCCC GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT   421 GACAGCAGAC GTGCACTGGC CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA   481 TCACTCTGTA CATCCACAAA CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA   541 AACTGCATTT CACCAGCCCC TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG   601 GGCGACCTCA GCCATCCCTT CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG   661 GGCTTCATTC TGCATGGTTG TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG   721 AGCAACTGAT AGCTGTCGCT GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC   781 TTTTTGACAT ACTTCGGGTA TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC   841 GCAAATACGC ATACTGTTAT CTGGCTTTTA NTAAGCCGGA TCCACGCGTT TACGCCCCGC   901 CCNGCCANTC ATCGCANNAN TGTTGTAATT CATTAAGCNT TCTGCCGANN NGGNANGCCN 961 TCACNAANGG NNNGANGNNN CNNGNNNCGN CNNCNNNNNN NNNN //
Primer: PRE_rev (Pr0250) Region: PRE,BSD,T2A,TetR Sequence file: RDB12129_A2H6c.seq
>07917_12129_A9JoF_PRE_rev_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGNNCANC ATAGTTAAGA ATACCAGTCA ATCTTTCACA    61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGTGC GGCCTTAGCC   121 CTCCCACACA TAACCAGAGG GCAGCAATTC ACGAATCCCA ACTGCCGTCG GCTGTCCATC   181 ACTGTCCTTC ACTATGGCTT TGATCCCAGG ATGCAGATCG AGAAGCACCT GTCGGCACCG   241 TCCGCAGGGG CTCAAGATGC CCCTGTTCTC ATTTCCGATC GCGACGATAC AAGTCAGGTT   301 GCCAGCTGCC GCAGCAGCAG CAGTGCCCAG CACCACGAGT TCTGCACAAG GTCCCCCAGT   361 AAAATGATAT ACATTGACAC CAGTGAAGAT GCGGCCGTCG CTAGAGAGAG CTGCGCTGGC   421 GACGCTGTAG TCTTCAGAGA TGGGGATGCT GTTGATTGTA GCCGTTGCTC TTTCAATGAG   481 GGTGGATTCT TCTTGAGACA AAGGCTTGGC CATGGGCCCG GGATTTTCCT CCACGTCACC   541 GCATGTTAGA AGACTTCCTC TGCCCTCGCC GGATCCGCTG TACGCGGACC CACTTTCACA   601 TTTAAGTTGT TTTTCTAATC CGCATATGAT CAATTCAAGG CCGAATAAGA AGGCTGGCTC   661 TGCACCTTGG TGATCAAATA ATTCGATAGC TTGTCGTAAT AATGGCGGCA TACTATCAGT   721 AGTAGGTGTT TCCCTTTCTT CTTTAGCGAC TTGATGCTCT TGATCTTCCA ATACGCAACC   781 TAAAGTAAAA TGCCCCACAG CGCTGANTGC ATATAATGCA TTCTCTANTG AAAAACCTTG   841 TTGGCATAAA AAGGCTAATT GATTTTCGAG ANTTTCATAC NGTTTTTCTG TANNCCGNGT   901 ANCNNAANGT ACTTTTNGCT NCATCGCGAT NNNNTANTAA AGCACATCTA AAACTTTTNA   961 NCNNTANTAC NNNAAAAANT CNTNGNCNNN NNTNNNNNNN TNNNNANNGN NNAANNNNNN 1021 NNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5'LTR,HIV-1 psi Sequence file: RDB12129_A2H6d.seq
>07917_12129_A9JoF_CMV_Forward_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC   121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA   181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG   241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC   301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG   361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG   421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT   481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC   541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC   601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA   661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT   721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGANCAC CGCACAGCAA GCGGCCGCTG   781 ATCTTCAGAN CTGGANGANG AGATATGANG GANAATTGGA GAAGTGAATT ATATAAATAT   841 AAAGTAGTAA AAATTGAANN NTTANGNNTA GCACCNACCA AGGNAAANAN AANANNGGTG   901 CNNANAGAAA AAAAGAGCAG NGGGAATAGG ANCTTNGTNN CTNNGGTNNT NNGGNNCANC 961 NNNAANCNCT NNNGNNNNNN NNNN //
Primer: RRE-M_R (Pr0651) Region: RRE Sequence file: RDB12129_A2H6e.seq
>07917_12129_A9JoF_RRE-M_R_B05_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT AGCCNNNNNN NNNNNGTTCT    61 GCTGCTGCAC TATACCAGAC AATAATTGTC TGGCCTGTAC CGTCAGCGTC ATTGACGCTG   121 CGCCCATAGT GCTTCCTGCT GCTCCCAAGA ACCCAAGGAA CAAAGCTCCT ATTCCCACTG   181 CTCTTTTTTC TCTCTGCACC ACTCTTCTCT TTGCCTTGGT GGGTGCTACT CCTAATGGTT   241 CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA ATTGTCCCTC ATATCTCCTC   301 CTCCAGGTCT GAAGATCAGC GGCCGCTTGC TGTGCGGTGG TCTTACTTTT GTTTTGCTCT   361 TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT CTATCCTTTG ATGCACACAA   421 TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT CTGATCCTGT CTGAAGGGAT   481 GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG TTTCTAACAG GCCAGGATTA   541 ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT GTTTTAATTT ATATTTTTTC   601 TTTCCCNNNN NNCTTAACCG AATTTTTTCC CATCGCGATC TAATTCTCCC CCGCTTAATA   661 NNNNNNCTCT CGCACNNNNN NCTCTCCTTC TAGCCTCCGC TANTCNAAAT TTTTGGCGTA   721 CTCNNNNNNC NCCGCCCCNT CNNNNCTNGN CGTGCGCGCT TCNNNNNNCN AGTCCTGCNT   781 CNANAGAGCT NCTCTGGTTT NCCNTNTCNN TTTCAAGTCC CTGTTCNNGC NCCNNTGCTA   841 NANANTTTNN ACNCTGACNN AANNGNNNTG ANGGANCTCT ANTTNNCNNN NNNNNNCNNC 901 NNACNGNNNC NNACNNCNTN NAAGCNNTNN NNNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: 3'LTR Sequence file: RDB12129_A2H6f.seq
>07917_12129_A9JoF_BGH_rev2_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGGGNNGG GNNNNNNNCA GATGGCTGGC AACTAGAAGG    61 CACAGTCGAG GCTGNTNNGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT   121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT   181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA   241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC   301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG   361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG   421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG   481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCNNTN NNGGAAGTAG CCTTGTGTGT   541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC   601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA   661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG   721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT   781 CTCGACGGTA TCGATGCGGG GANGCGGCCC AAAGGGANAT CCGANTCGTC TGANGGCGAA   841 NGCGAAGANG CGNAANANGC CGCANANCCG GCAGCANGCC GCGGGAANGA AGGTCCGCTN   901 GATTGANGGC CGAANNGNNN NNANCAGAAN GGNCNTCCNN CNCANNNTCC NNGNGGNNNC 961 ACNNGNNANC NNNNNNNGNN ANNGNNNNNN NNNN //
Primer: SV40pro_F (Pr0110) Region: Zeo Sequence file: RDB12129_A2H6g.seq
>07917_12129_A9JoF_SV40pro-F_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNANNNNN NNGNNGGNNG CTTTTTTGGA GGCCTAGGCT    61 TTTGCANANN GCTCCCGGGA GCTTNNNTAT CCATTTTCGG ATCTGATCAG CACGTGTTGA   121 CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC   181 CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT   241 CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG   301 TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA   361 CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA   421 GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA   481 GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC   541 CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG   601 GTTGGGCTTC GGAATCGTTT TCCGGGACGC NNNNNGGATG ATCCTCCAGC GCGGGGATCT   661 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA   721 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCNNTGCATT CTAGTTGTGG   781 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGANCT CTAGCTAGAG   841 CTTGNCGTAA TCATGGTCAT AGCTGTTTTC CTGTGTGAAA TTGTTATCCG CNCACNATTC   901 CACACAACAT ACGANCCGGA ANCATAAAGT GNNAANGNCN GGGGGTGCCT AATGAGNGAN   961 CNNNNTCACA TTANNNGCGT TGCGNNNNNT GNCCNCTTTN NNGNNNNGNN AANNNNNNNN 1021 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl