Resource data sheet
Prev. |  Lentiviral vector plasmid > 

CS-RfA-ETBsd

Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Bsd marker driven by EF-1 alpha promoter.

Catalog number RDB07917
Resource name CS-RfA-ETBsd
Clone info. Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with TetR-2A-Bsd marker driven by EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CS-RfA-ETBsd ()
Selectable markers Lentivirus transfection: Bsd^r. E. coli: Amp^r, Cm^r, ccdB. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07917 CS-RfA-ETBsd DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07917z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB07917.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12129_A2H6p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: CmR,attR1,cPPT_CTS | Primer: Cm_M_R | Sequence: RDB12129_A2H6a.seq
>07917_12129_A9JoF_Cm_M_R_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NTATNNCCNG CTNNNCGTCT TTCNTTGCCN TACGGAATTC
   61 CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT
  121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT
  181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC
  241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT
  301 CGACGGATCC TAACTCAAAA TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC
  361 TGGGGTGCCT AATGCGGCCG CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA
  421 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT
  481 CGTTCAGCTT TTTTGTACAA ACTTGTGATA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA
  541 TAAACCCGAA AATTTTGAAT TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT
  601 GTTGCTATTA TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT
  661 TTAAAATTGT GGATGAATAC TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC
  721 TCTGTCTCTC TCTCCACCTT CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT
  781 TGGGANGTGG GTCTGAAACG ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA
  841 AAGTACAGCA AAAACTATTC TTAAACCTAN NNAGCCTCCN ANTATCATTA TGAATAATTT
  901 TATATACCAN AGCCNATTTG TTATGTTAAA CNNATTNNNC NAACTTGCCN ATTTATCTAA
  961 NNNCNNNANT TCNNNNNCNT TCNTTTTNNN NNNNNNTTNN NNANNNNTNN NNNTNNANNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NN
//
Region: EF1alpha pro,attR2,ccdB | Primer: EF1a-N_R | Sequence: RDB12129_A2H6b.seq
>07917_12129_A9JoF_EF1a-N_R_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNGNNNNNNN GGCGNNGTGC GCTCTGCCCN CTGNCGGGCA CCGGNNCCTC
   61 ACGAAGCTTG AATTATCACC ACTTTGTACA AGAAAGCTGA ACGAGAAACG TAAAATGATA
  121 TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA
  181 CACAACATAT CCAGTCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC
  241 ATTTATATTC CCCAGAACAT CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG
  301 AGATCAGCCA CTTCTTCCCC GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC
  361 ATGCGCCAGC TTTCATCCCC GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT
  421 GACAGCAGAC GTGCACTGGC CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA
  481 TCACTCTGTA CATCCACAAA CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA
  541 AACTGCATTT CACCAGCCCC TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG
  601 GGCGACCTCA GCCATCCCTT CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG
  661 GGCTTCATTC TGCATGGTTG TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG
  721 AGCAACTGAT AGCTGTCGCT GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC
  781 TTTTTGACAT ACTTCGGGTA TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC
  841 GCAAATACGC ATACTGTTAT CTGGCTTTTA NTAAGCCGGA TCCACGCGTT TACGCCCCGC
  901 CCNGCCANTC ATCGCANNAN TGTTGTAATT CATTAAGCNT TCTGCCGANN NGGNANGCCN
  961 TCACNAANGG NNNGANGNNN CNNGNNNCGN CNNCNNNNNN NNNNNCNNNN NNNNCNTNNN
 1021 NNNNNNNNNT NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN NNNNNNNNNN NNNNNNNNNN NNNN
//
Region: PRE,BSD,T2A,TetR | Primer: PRE_rev | Sequence: RDB12129_A2H6c.seq
>07917_12129_A9JoF_PRE_rev_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGNNCANC ATAGTTAAGA ATACCAGTCA ATCTTTCACA
   61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGTGC GGCCTTAGCC
  121 CTCCCACACA TAACCAGAGG GCAGCAATTC ACGAATCCCA ACTGCCGTCG GCTGTCCATC
  181 ACTGTCCTTC ACTATGGCTT TGATCCCAGG ATGCAGATCG AGAAGCACCT GTCGGCACCG
  241 TCCGCAGGGG CTCAAGATGC CCCTGTTCTC ATTTCCGATC GCGACGATAC AAGTCAGGTT
  301 GCCAGCTGCC GCAGCAGCAG CAGTGCCCAG CACCACGAGT TCTGCACAAG GTCCCCCAGT
  361 AAAATGATAT ACATTGACAC CAGTGAAGAT GCGGCCGTCG CTAGAGAGAG CTGCGCTGGC
  421 GACGCTGTAG TCTTCAGAGA TGGGGATGCT GTTGATTGTA GCCGTTGCTC TTTCAATGAG
  481 GGTGGATTCT TCTTGAGACA AAGGCTTGGC CATGGGCCCG GGATTTTCCT CCACGTCACC
  541 GCATGTTAGA AGACTTCCTC TGCCCTCGCC GGATCCGCTG TACGCGGACC CACTTTCACA
  601 TTTAAGTTGT TTTTCTAATC CGCATATGAT CAATTCAAGG CCGAATAAGA AGGCTGGCTC
  661 TGCACCTTGG TGATCAAATA ATTCGATAGC TTGTCGTAAT AATGGCGGCA TACTATCAGT
  721 AGTAGGTGTT TCCCTTTCTT CTTTAGCGAC TTGATGCTCT TGATCTTCCA ATACGCAACC
  781 TAAAGTAAAA TGCCCCACAG CGCTGANTGC ATATAATGCA TTCTCTANTG AAAAACCTTG
  841 TTGGCATAAA AAGGCTAATT GATTTTCGAG ANTTTCATAC NGTTTTTCTG TANNCCGNGT
  901 ANCNNAANGT ACTTTTNGCT NCATCGCGAT NNNNTANTAA AGCACATCTA AAACTTTTNA
  961 NCNNTANTAC NNNAAAAANT CNTNGNCNNN NNTNNNNNNN TNNNNANNGN NNAANNNNNN
 1021 NNNNGNNNNC NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNN
//
Region: CMV pro,5'LTR,HIV-1 psi | Primer: CMV_Forward | Sequence: RDB12129_A2H6d.seq
>07917_12129_A9JoF_CMV_Forward_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGANCAC CGCACAGCAA GCGGCCGCTG
  781 ATCTTCAGAN CTGGANGANG AGATATGANG GANAATTGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAATTGAANN NTTANGNNTA GCACCNACCA AGGNAAANAN AANANNGGTG
  901 CNNANAGAAA AAAAGAGCAG NGGGAATAGG ANCTTNGTNN CTNNGGTNNT NNGGNNCANC
  961 NNNAANCNCT NNNGNNNNNN NNNNNNNNNN NNCNNNNNNN NNNNNNCNNN NNNNNNNNNT
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNN
//
Region: RRE | Primer: RRE-M_R | Sequence: RDB12129_A2H6e.seq
>07917_12129_A9JoF_RRE-M_R_B05_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT AGCCNNNNNN NNNNNGTTCT
   61 GCTGCTGCAC TATACCAGAC AATAATTGTC TGGCCTGTAC CGTCAGCGTC ATTGACGCTG
  121 CGCCCATAGT GCTTCCTGCT GCTCCCAAGA ACCCAAGGAA CAAAGCTCCT ATTCCCACTG
  181 CTCTTTTTTC TCTCTGCACC ACTCTTCTCT TTGCCTTGGT GGGTGCTACT CCTAATGGTT
  241 CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA ATTGTCCCTC ATATCTCCTC
  301 CTCCAGGTCT GAAGATCAGC GGCCGCTTGC TGTGCGGTGG TCTTACTTTT GTTTTGCTCT
  361 TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT CTATCCTTTG ATGCACACAA
  421 TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT CTGATCCTGT CTGAAGGGAT
  481 GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG TTTCTAACAG GCCAGGATTA
  541 ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT GTTTTAATTT ATATTTTTTC
  601 TTTCCCNNNN NNCTTAACCG AATTTTTTCC CATCGCGATC TAATTCTCCC CCGCTTAATA
  661 NNNNNNCTCT CGCACNNNNN NCTCTCCTTC TAGCCTCCGC TANTCNAAAT TTTTGGCGTA
  721 CTCNNNNNNC NCCGCCCCNT CNNNNCTNGN CGTGCGCGCT TCNNNNNNCN AGTCCTGCNT
  781 CNANAGAGCT NCTCTGGTTT NCCNTNTCNN TTTCAAGTCC CTGTTCNNGC NCCNNTGCTA
  841 NANANTTTNN ACNCTGACNN AANNGNNNTG ANGGANCTCT ANTTNNCNNN NNNNNNCNNC
  901 NNACNGNNNC NNACNNCNTN NAAGCNNTNN NNNNNNNNTT TATNNNNNNT NNNNCANNGN
961 GTNCNNTNGT NNNNNNNNNN NNNN
//
Region: 3'LTR | Primer: BGH_rev2 | Sequence: RDB12129_A2H6f.seq
>07917_12129_A9JoF_BGH_rev2_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGGGNNGG GNNNNNNNCA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGNTNNGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
  121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
  301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
  361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
  421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
  481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCNNTN NNGGAAGTAG CCTTGTGTGT
  541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
  601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
  661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
  721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
  781 CTCGACGGTA TCGATGCGGG GANGCGGCCC AAAGGGANAT CCGANTCGTC TGANGGCGAA
  841 NGCGAAGANG CGNAANANGC CGCANANCCG GCAGCANGCC GCGGGAANGA AGGTCCGCTN
  901 GATTGANGGC CGAANNGNNN NNANCAGAAN GGNCNTCCNN CNCANNNTCC NNGNGGNNNC
  961 ACNNGNNANC NNNNNNNGNN ANNGNNNNNN NNNNNNCNNN NNNNNNNNNN NNNNNNNNNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNNNNNNNN
//
Region: Zeo | Primer: SV40pro_F | Sequence: RDB12129_A2H6g.seq
>07917_12129_A9JoF_SV40pro-F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNANNNNN NNGNNGGNNG CTTTTTTGGA GGCCTAGGCT
   61 TTTGCANANN GCTCCCGGGA GCTTNNNTAT CCATTTTCGG ATCTGATCAG CACGTGTTGA
  121 CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC
  181 CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT
  241 CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG
  301 TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA
  361 CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA
  421 GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA
  481 GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC
  541 CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG
  601 GTTGGGCTTC GGAATCGTTT TCCGGGACGC NNNNNGGATG ATCCTCCAGC GCGGGGATCT
  661 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA
  721 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCNNTGCATT CTAGTTGTGG
  781 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGANCT CTAGCTAGAG
  841 CTTGNCGTAA TCATGGTCAT AGCTGTTTTC CTGTGTGAAA TTGTTATCCG CNCACNATTC
  901 CACACAACAT ACGANCCGGA ANCATAAAGT GNNAANGNCN GGGGGTGCCT AATGAGNGAN
  961 CNNNNTCACA TTANNNGCGT TGCGNNNNNT GNCCNCTTTN NNGNNNNGNN AANNNNNNNN
 1021 NNNNNCNNNN NNNNANNNNA NNNNNNNNNN NNNNNNGNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

reference Isaji, T., An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J. Biol. Chem. 289 (30): 20694-20705 (2014). PMID 24895123.
user report Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219.
user report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
user report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
user report Hikasa, H., Merlin/NF2-Lin28B-let-7 Is a Tumor-Suppressive Pathway that Is Cell-Density Dependent and Hippo Independent. Cell Rep. 14 (12): 2950-2961 (2016). PMID 26997273.
user report Hirokawa, T., CBP-93872 inhibits NBS1-mediated ATR activation, abrogating maintenance of the DNA double-strand break-specific G2 checkpoint. Cancer Res. 74 (14): 3880-3889 (2014). PMID 24876101.

2019.11.15

GNP_filter3_RDBDEP_html_191106.pl