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pENTR4-H1tetOx1 (#RDB07916)

Expression vector of RNAi driven by human H1 promoter.


Drawn by SnapGene® software
Sequence (full) RDB07916hts01.seq
Remarks, protocol and/or map (pdf) RDB07916.pdf
Publication Suga, H., Nature 480 (7375): 57-62 (2011). [link to RRC of NBRP]
Test sheet RDB12397_B3Ftp1-3.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of RNAi driven by human H1 promoter. Frequently requested along with Gateway(R) destination vectors for constructing short hairpin RNA (shRNA) expressing vectors.
Vector backbone pENTR4 (Plasmid)
Selectable markers Kan^r
Gene/insert name Human RPPH1 genomic DNA
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07916 pENTR4-H1tetOx1 DNA solution

How to cite this biological resource

Materials & Methods section:

The pENTR4-H1tetOx1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07916).

Reference section:

Suga, H., Kadoshima, T., Minaguchi, M., Ohgushi, M., Soen, M., Nakano, T., Takata, N., Wataya, T., Muguruma, K., Miyoshi, H., Yonemura, S., Oiso, Y., Sasai, Y., Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480 (7375): 57-62 (2011). PMID 22080957. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Suga, H., Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480 (7375): 57-62 (2011). PMID 22080957. [link to RRC of NBRP]
reference Noura, M., Suppression of super-enhancer-driven TAL1 expression by KLF4 in T-cell acute lymphoblastic leukemia.
29 June 2023, PREPRINT (Version 1) available at Research Square https://doi.org/10.21203/rs.3.rs-3069900/v1
reference Takata, N., Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9. Hum. Gene Ther. 27 (6): 436-450 (2016). PMID 26839115.
user_report Watanabe, K., Inhibition of the galactosyltransferase C1GALT1 reduces osteosarcoma cell proliferation by interfering with ERK signaling and cell cycle progression. Cancer Gene Ther. 2024 Apr 15. doi: 10.1038/s41417-024-00773-9. Epub ahead of print. PMID 38622340. [link to RRC of NBRP]
user_report Noura, M., NUP98-BPTF promotes oncogenic transformation through PIM1 upregulation. 29 Oct 2023, PREPRINT (Version 1) available at Research Square https://doi.org/10.21203/rs.3.rs-3498291/v1
user_report Hada, M., Highly rigid H3.1/H3.2-H3K9me3 domains set a barrier for cell fate reprogramming in trophoblast stem cells. Genes Dev. 36 (1-2): 84-102 (2022). PMID 34992147. [link to RRC of NBRP]
user_report Matsui, Y., Chlorambucil-conjugated PI-polyamides (Chb-M'), a transcription inhibitor of RUNX family, has an anti-tumor activity against SHH-type medulloblastoma with p53 mutation. Biochem. Biophys. Res. Commun. 620: 150-157 (2022). PMID 35792512. [link to RRC of NBRP]
user_report Hattori, E.Y., A RUNX-targeted gene switch-off approach modulates the BIRC5/PIF1-p21 pathway and reduces glioblastoma growth in mice. Commun. Biol. 5 (1): 939 (2022). PMID 36085167. [link to RRC of NBRP]
user_report Nakatani, K., Inhibition of CDK4/6 and autophagy synergistically induces apoptosis in t(8;21) acute myeloid leukemia cells. Int. J. Hematol. 113 (2): 243-253 (2021). PMID 33068248. [link to RRC of NBRP]
user_report Kohrogi, K., LSD1 defines erythroleukemia metabolism by controlling the lineage-specific transcription factors GATA1 and C/EBPalpha. Blood Adv. 5 (9): 2305-2318 (2021). PMID 33929501. [link to RRC of NBRP]
user_report Morita, K., Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Cell 181 (3): 702-715.e20 (2020). PMID 32315619. [link to RRC of NBRP]
user_report Noura, M., Pivotal role of DPYSL2A in KLF4-mediated monocytic differentiation of acute myeloid leukemia cells. Sci. Rep. 10 (1): 20245 (2020). PMID 33219287. [link to RRC of NBRP]
user_report Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219. [link to RRC of NBRP]
user_report Mitsuda, Y., RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci. Rep. 8 (1): 6423 (2018). PMID 29686309. [link to RRC of NBRP]
user_report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640. [link to RRC of NBRP]
user_report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243. [link to RRC of NBRP]
user_report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Adv. 1: 1440-1451 (2017). PMID 29296785. [link to RRC of NBRP]
user_report Hashizume, O., Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects. Sci. Rep. 5: 10434 (2015). PMID 26000717. [link to RRC of NBRP]
user_report Hirokawa, T., CBP-93872 inhibits NBS1-mediated ATR activation, abrogating maintenance of the DNA double-strand break-specific G2 checkpoint. Cancer Res. 74 (14): 3880-3889 (2014). PMID 24876101. [link to RRC of NBRP]