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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pENTR4-H1tetOx1

Expression vector of RNAi driven by human H1 promoter.

Catalog number RDB07916
Resource name pENTR4-H1tetOx1
Clone info. Expression vector of RNAi driven by human H1 promoter. Frequently requested along with Gateway(R) destination vectors for constructing short hairpin RNA (shRNA) expressing vectors.
Vector backbone pENTR4 (Plasmid)
Selectable markers Kan^r
Gene/insert name Human RPPH1 genomic DNA
Depositor|Developer Miyoshi, Hiroyuki |
 
Other clones in our bank human RPPH1 (NCBI Gene 85495) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07916 pENTR4-H1tetOx1 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB07916z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB07916.pdf

Original reference

original Suga, H., Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480 (7375): 57-62 (2011). PMID 22080957.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12397_A4L8p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pDONR_R
Sequence file: RDB12397_A4L8a.seq check
>07916_12397_A4L8_pDONR_R_D10_10.ab1
    1 TGGGCCGAAG GCGCTGGATG GCAATAATGA TTTTATTTTG ACTGATAGCT GCACCTGTTC
   61 GTTGCAACAA ATTGATAAGC AATGCTTTCT TATAATGCCA ACTTTGTACA AGAAAGCTGG
  121 GTCTAGATAT CTCGACTCTA GAGGATCCCC GGCAGATCTC TATCACTGAT AGGGACTTAT
  181 AAGATTCCCA AATCCAAAGA CATTTCACGT TTATGGTGAT TTCCCAGAAC ACATAGCGAC
  241 ATGCAAATAT TGCAGGGCGC CACTCCCCTG TCCCTCACAG CCATCTTCCT GCCAGGGCGC
  301 ACGCGCGCTG GGTGTTCCCG CCTAGTGACA CTGGGCCCGC GATTCCTTGG AGCGGGTTGA
  361 TGACGTCAGC GTTCGAATTC GGTACCGGAT CCAGTCGACT GAATTGGTTC CCATGGTGGA
  421 GCCTGCTTTT TTGTACAAAG TTGGCATTAT AAAAAAGCAT TGCTTATCAA TTTGTTGCAA
  481 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGG GGCCCGAGCT TAAGTAACTA
  541 ACTAACAGGA AGAGTTTGTA GAAACGCAAA AAGGCCATCC GTCAGGATGG CCTTCTGCTT
  601 AGTTTGATGC CTGGCAGTTT ATGGCGGGCG TCCTGCCCGC CACCCTCCGG GCCGTTGCTT
  661 CACAACGTTC AAATCCGCTC CCGGCGGATT TGTCCTACTC AGGAGAGCGT TCACCGACAA
  721 AACAACAGAT AAAACGAAAG GCCCAGTCTT CCGACTGAGC CTTTCGTTTT ATTTGATGCC
  781 TGGCAGTTCC CTACTCTCGC TTAGTAGTTA GACGTCCCCG AGATCCATGC TAGCGGTAAT
  841 ACGGTTATCC ACAGAATTCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC
  901 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC
  961 CTGACGAGCA TCACAAAAAT TCGACGCTCA AGTCAGAGGT GGCGAAACCC CGACAGGACT
 1021 ATAAAGAATA CCAAGGCGTT CCCCCCTGGA AGCTCCCTTC GTGCGCTCTC CTGTTCCGGA
 1081 CCCTGCCGGC TTACCGGGAT TACCTGTTCC GGCCCTTTCT CCCATACGGG AAGCGGTGGG
 1141 CGCTTTACTT CAATAGGCAT TCACACGCCC TGTGATTAGA GAGTATATAT CCTTCATCAC
1201 AAGAT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Suga, H., Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480 (7375): 57-62 (2011). PMID 22080957.
reference Takata, N., Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9. Hum. Gene Ther. 27 (6): 436-450 (2016). PMID 26839115.
user report Morita, K., Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Cell 181 (3): 702-715.e20 (2020). PMID 32315619.
user report Nakatani, K., Inhibition of CDK4/6 and autophagy synergistically induces apoptosis in t(8;21) acute myeloid leukemia cells. Int. J. Hematol.
. 2020 Oct 17. doi: 10.1007/s12185-020-03015-4. Online ahead of print. PMID 33068248.
user report Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219.
user report Mitsuda, Y., RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci. Rep. 8 (1): 6423 (2018). PMID 29686309.
user report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
user report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
user report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Adv. 1: 1440-1451 (2017). PMID 29296785.
user report Hashizume, O., Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects. Sci. Rep. 5: 10434 (2015). PMID 26000717.
user report Hirokawa, T., CBP-93872 inhibits NBS1-mediated ATR activation, abrogating maintenance of the DNA double-strand break-specific G2 checkpoint. Cancer Res. 74 (14): 3880-3889 (2014). PMID 24876101.

2020.12.30

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