Resource data sheet
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pENTR4-H1tetOx1

Expression vector of RNAi driven by human H1 promoter.

Catalog number RDB07916
Resource name pENTR4-H1tetOx1
Clone info. Expression vector of RNAi driven by human H1 promoter. Frequently requested along with Gateway(R) destination vectors for constructing short hairpin RNA (shRNA) expressing vectors.
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12397_A4L8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pDONR_R RDB12397_A4L8a.seq
>07916_12397_A4L8_pDONR_R_D10_10.ab1
    1 TGGGCCGAAG GCGCTGGATG GCAATAATGA TTTTATTTTG ACTGATAGCT GCACCTGTTC
   61 GTTGCAACAA ATTGATAAGC AATGCTTTCT TATAATGCCA ACTTTGTACA AGAAAGCTGG
  121 GTCTAGATAT CTCGACTCTA GAGGATCCCC GGCAGATCTC TATCACTGAT AGGGACTTAT
  181 AAGATTCCCA AATCCAAAGA CATTTCACGT TTATGGTGAT TTCCCAGAAC ACATAGCGAC
  241 ATGCAAATAT TGCAGGGCGC CACTCCCCTG TCCCTCACAG CCATCTTCCT GCCAGGGCGC
  301 ACGCGCGCTG GGTGTTCCCG CCTAGTGACA CTGGGCCCGC GATTCCTTGG AGCGGGTTGA
  361 TGACGTCAGC GTTCGAATTC GGTACCGGAT CCAGTCGACT GAATTGGTTC CCATGGTGGA
  421 GCCTGCTTTT TTGTACAAAG TTGGCATTAT AAAAAAGCAT TGCTTATCAA TTTGTTGCAA
  481 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGG GGCCCGAGCT TAAGTAACTA
  541 ACTAACAGGA AGAGTTTGTA GAAACGCAAA AAGGCCATCC GTCAGGATGG CCTTCTGCTT
  601 AGTTTGATGC CTGGCAGTTT ATGGCGGGCG TCCTGCCCGC CACCCTCCGG GCCGTTGCTT
  661 CACAACGTTC AAATCCGCTC CCGGCGGATT TGTCCTACTC AGGAGAGCGT TCACCGACAA
  721 AACAACAGAT AAAACGAAAG GCCCAGTCTT CCGACTGAGC CTTTCGTTTT ATTTGATGCC
  781 TGGCAGTTCC CTACTCTCGC TTAGTAGTTA GACGTCCCCG AGATCCATGC TAGCGGTAAT
  841 ACGGTTATCC ACAGAATTCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC
  901 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC
  961 CTGACGAGCA TCACAAAAAT TCGACGCTCA AGTCAGAGGT GGCGAAACCC CGACAGGACT
 1021 ATAAAGAATA CCAAGGCGTT CCCCCCTGGA AGCTCCCTTC GTGCGCTCTC CTGTTCCGGA
 1081 CCCTGCCGGC TTACCGGGAT TACCTGTTCC GGCCCTTTCT CCCATACGGG AAGCGGTGGG
 1141 CGCTTTACTT CAATAGGCAT TCACACGCCC TGTGATTAGA GAGTATATAT CCTTCATCAC
1201 AAGAT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

original Suga, H., Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480 (7375): 57-62 (2011). PMID 22080957.
reference Takata, N., Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9. Hum. Gene Ther. 27 (6): 436-450 (2016). PMID 26839115.
user report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Advances 1: 1440-1451 (2017).
user report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
user report Hashizume, O., Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects. Sci. Rep. 5: 10434 (2015). PMID 26000717.
user report Hirokawa, T., CBP-93872 inhibits NBS1-mediated ATR activation, abrogating maintenance of the DNA double-strand break-specific G2 checkpoint. Cancer Res. 74 (14): 3880-3889 (2014). PMID 24876101.

2017.10.23

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