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pENTR4-H1tetOx1

Expression vector of RNAi driven by human H1 promoter.

Catalog number RDB07916
Resource name pENTR4-H1tetOx1
Clone info. Expression vector of RNAi driven by human H1 promoter. Frequently requested along with Gateway(R) destination vectors for constructing short hairpin RNA (shRNA) expressing vectors.
Vector backbone pENTR4 (Plasmid)
Selectable markers Kan^r
Gene/insert name Human RPPH1 genomic DNA
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank
External Database
Human RPPH1
          Reference sequence
            
           
          Sequence (full) RDB07916zzk02.seq provided by the depositor
          Remarks, protocol and/or map (pdf) RDB07916.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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          提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
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          RDB07916 pENTR4-H1tetOx1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pENTR4-H1tetOx1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07916).

          Reference section:

          Suga, H., Kadoshima, T., Minaguchi, M., Ohgushi, M., Soen, M., Nakano, T., Takata, N., Wataya, T., Muguruma, K., Miyoshi, H., Yonemura, S., Oiso, Y., Sasai, Y., Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480 (7375): 57-62 (2011). PMID 22080957.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Lentivirus Vector (English text)
          Featured content Lentivirus Vector (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12397_A4L8p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: H1_pro-C_R (Pr1210)
          Region: H1 pro, attL1, rrnB T2_T1 ter, ori
          Sequence file: RDB12397_A4L8b.seq check
          >7916_12397_B2E9F_H1_pro-C_R_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNCN NTCNNNGCNN TCTTCCTGCC NGGGCGCACG CGCGCTGGGT
             61 GTTCCCGCCT AGTGACACTG GGCCCGCGAT TCCTTGGAGC GGGTTGATGA CGTCAGCGTT
            121 CGAATTCGGT ACCGGATCCA GTCGACTGAA TTGGTTCCCA TGGTGGAGCC TGCTTTTTTG
            181 TACAAAGTTG GCATTATAAA AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT
            241 ATCAGTCAAA ATAAAATCAT TATTTGGGGC CCGAGCTTAA GTAACTAACT AACAGGAAGA
            301 GTTTGTAGAA ACGCAAAAAG GCCATCCGTC AGGATGGCCT TCTGCTTAGT TTGATGCCTG
            361 GCAGTTTATG GCGGGCGTCC TGCCCGCCAC CCTCCGGGCC GTTGCTTCAC AACGTTCAAA
            421 TCCGCTCCCG GCGGATTTGT CCTACTCAGG AGAGCGTTCA CCGACAAACA ACAGATAAAA
            481 CGAAAGGCCC AGTCTTCCGA CTGAGCCTTT CGTTTTATTT GATGCCTGGC AGTTCCCTAC
            541 TCTCGCTTAG TAGTTAGACG TCCCCGAGAT CCATGCTAGC GGTAATACGG TTATCCACAG
            601 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC
            661 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
            721 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCANGCGT
            781 TTCCCCCTGN AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
            841 TGTCCGCCTT TCTCCCTTCN GGAAGCGNGG CGCTTTCTCA TAGCTCANGC TGTNNNATCT
            901 CAGTTCGGNG TANGTCGTTC GCTCCNAGCT GGGCTGTGNG CACNANNCCC CCGTTCANCC
          961 NNANCGCTGC NNCTNATCCG GTANNTATCN TCTNGANNNN NNNNNN
          //
          Primer: KanR-N_R (Pr0530)
          Region: KanR, attL2, tetO, H1 pro
          Sequence file: RDB12397_A4L8c.seq check
          >7916_12397_B2E9F_KanR-N_R_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNCNN NATAAATNNG CATCNNTGTT GGAATTTAAT CGCGGCCTCG
             61 ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA
            121 GTTTTATTGT TCATGATGAT ATATTTTTAT CTTGTGCAAT GTAACATCAG AGATTTTGAG
            181 ACACGGGCCA GAGCTGCAGC TGGATGGCAA ATAATGATTT TATTTTGACT GATAGTGACC
            241 TGTTCGTTGC AACAAATTGA TAAGCAATGC TTTCTTATAA TGCCAACTTT GTACAAGAAA
            301 GCTGGGTCTA GATATCTCGA CTCTAGAGGA TCCCCGGCAG ATCTCTATCA CTGATAGGGA
            361 CTTATAAGAT TCCCAAATCC AAAGACATTT CACGTTTATG GTGATTTCCC AGAACACATA
            421 GCGACATGCA AATATTGCAG GGCGCCACTC CCCTGTCCCT CACAGCCATC TTCCTGCCAG
            481 GGCGCACGCG CGCTGGGTGT TCCCGCCTAG TGACACTGGG CCCGCGATTC CTTGGAGCGG
            541 GTTGANGACN TCAGCGTTCG AATTCGGTAC NNGATCCNGT CNACTGANTT GNNTNCNNNT
          601 GGTGNAGCCT GCTTTTTNGN ANNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Suga, H., Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480 (7375): 57-62 (2011). PMID 22080957.
          reference Takata, N., Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9. Hum. Gene Ther. 27 (6): 436-450 (2016). PMID 26839115.
          user_report Hada, M., Highly rigid H3.1/H3.2-H3K9me3 domains set a barrier for cell fate reprogramming in trophoblast stem cells. Genes Dev. 36 (1-2): 84-102 (2022). PMID 34992147.
          user_report Matsui, Y., Chlorambucil-conjugated PI-polyamides (Chb-M'), a transcription inhibitor of RUNX family, has an anti-tumor activity against SHH-type medulloblastoma with p53 mutation. Biochem. Biophys. Res. Commun. 620: 150-157 (2022). PMID 35792512.
          user_report Hattori, E.Y., A RUNX-targeted gene switch-off approach modulates the BIRC5/PIF1-p21 pathway and reduces glioblastoma growth in mice. Commun. Biol. 5 (1): 939 (2022). PMID 36085167.
          user_report Nakatani, K., Inhibition of CDK4/6 and autophagy synergistically induces apoptosis in t(8;21) acute myeloid leukemia cells. Int. J. Hematol. 113 (2): 243-253 (2021). PMID 33068248.
          user_report Kohrogi, K., LSD1 defines erythroleukemia metabolism by controlling the lineage-specific transcription factors GATA1 and C/EBPalpha. Blood Adv. 5 (9): 2305-2318 (2021). PMID 33929501.
          user_report Morita, K., Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Cell 181 (3): 702-715.e20 (2020). PMID 32315619.
          user_report Noura, M., Pivotal role of DPYSL2A in KLF4-mediated monocytic differentiation of acute myeloid leukemia cells. Sci. Rep. 10 (1): 20245 (2020). PMID 33219287.
          user_report Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219.
          user_report Mitsuda, Y., RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci. Rep. 8 (1): 6423 (2018). PMID 29686309.
          user_report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
          user_report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
          user_report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Adv. 1: 1440-1451 (2017). PMID 29296785.
          user_report Hashizume, O., Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects. Sci. Rep. 5: 10434 (2015). PMID 26000717.
          user_report Hirokawa, T., CBP-93872 inhibits NBS1-mediated ATR activation, abrogating maintenance of the DNA double-strand break-specific G2 checkpoint. Cancer Res. 74 (14): 3880-3889 (2014). PMID 24876101.

          2023.05.22

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