Expression vector of RNAi driven by human H1 promoter.
|
Other clones in our bank
External Database Human RPPH1 Reference sequence |
Sequence (full) ![]() |
RDB07916zzk02.seq provided by the depositor |
---|---|
Remarks, protocol and/or map (pdf) | RDB07916.pdf |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
---|---|
Ordering | Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Please visit Information of Request for Distribution.[link] |
提供条件 | 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 |
---|---|
提供依頼 | 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB07916 | pENTR4-H1tetOx1 | DNA solution |
Materials & Methods section:
The pENTR4-H1tetOx1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07916). |
Reference section:
Suga, H., Kadoshima, T., Minaguchi, M., Ohgushi, M., Soen, M., Nakano, T., Takata, N., Wataya, T., Muguruma, K., Miyoshi, H., Yonemura, S., Oiso, Y., Sasai, Y., Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480 (7375): 57-62 (2011). PMID 22080957. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Lentivirus Vector (English text) |
---|---|
Featured content | Lentivirus Vector (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB12397_A4L8p1-3.pdf ![]() |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: H1_pro-C_R (Pr1210) Region: H1 pro, attL1, rrnB T2_T1 ter, ori Sequence file: RDB12397_A4L8b.seq ![]() |
---|
>7916_12397_B2E9F_H1_pro-C_R_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNCN NTCNNNGCNN TCTTCCTGCC NGGGCGCACG CGCGCTGGGT 61 GTTCCCGCCT AGTGACACTG GGCCCGCGAT TCCTTGGAGC GGGTTGATGA CGTCAGCGTT 121 CGAATTCGGT ACCGGATCCA GTCGACTGAA TTGGTTCCCA TGGTGGAGCC TGCTTTTTTG 181 TACAAAGTTG GCATTATAAA AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT 241 ATCAGTCAAA ATAAAATCAT TATTTGGGGC CCGAGCTTAA GTAACTAACT AACAGGAAGA 301 GTTTGTAGAA ACGCAAAAAG GCCATCCGTC AGGATGGCCT TCTGCTTAGT TTGATGCCTG 361 GCAGTTTATG GCGGGCGTCC TGCCCGCCAC CCTCCGGGCC GTTGCTTCAC AACGTTCAAA 421 TCCGCTCCCG GCGGATTTGT CCTACTCAGG AGAGCGTTCA CCGACAAACA ACAGATAAAA 481 CGAAAGGCCC AGTCTTCCGA CTGAGCCTTT CGTTTTATTT GATGCCTGGC AGTTCCCTAC 541 TCTCGCTTAG TAGTTAGACG TCCCCGAGAT CCATGCTAGC GGTAATACGG TTATCCACAG 601 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 661 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 721 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCANGCGT 781 TTCCCCCTGN AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 841 TGTCCGCCTT TCTCCCTTCN GGAAGCGNGG CGCTTTCTCA TAGCTCANGC TGTNNNATCT 901 CAGTTCGGNG TANGTCGTTC GCTCCNAGCT GGGCTGTGNG CACNANNCCC CCGTTCANCC 961 NNANCGCTGC NNCTNATCCG GTANNTATCN TCTNGANNNN NNNNNN // |
Primer: KanR-N_R (Pr0530) Region: KanR, attL2, tetO, H1 pro Sequence file: RDB12397_A4L8c.seq ![]() |
>7916_12397_B2E9F_KanR-N_R_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNCNN NATAAATNNG CATCNNTGTT GGAATTTAAT CGCGGCCTCG 61 ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA 121 GTTTTATTGT TCATGATGAT ATATTTTTAT CTTGTGCAAT GTAACATCAG AGATTTTGAG 181 ACACGGGCCA GAGCTGCAGC TGGATGGCAA ATAATGATTT TATTTTGACT GATAGTGACC 241 TGTTCGTTGC AACAAATTGA TAAGCAATGC TTTCTTATAA TGCCAACTTT GTACAAGAAA 301 GCTGGGTCTA GATATCTCGA CTCTAGAGGA TCCCCGGCAG ATCTCTATCA CTGATAGGGA 361 CTTATAAGAT TCCCAAATCC AAAGACATTT CACGTTTATG GTGATTTCCC AGAACACATA 421 GCGACATGCA AATATTGCAG GGCGCCACTC CCCTGTCCCT CACAGCCATC TTCCTGCCAG 481 GGCGCACGCG CGCTGGGTGT TCCCGCCTAG TGACACTGGG CCCGCGATTC CTTGGAGCGG 541 GTTGANGACN TCAGCGTTCG AATTCGGTAC NNGATCCNGT CNACTGANTT GNNTNCNNNT 601 GGTGNAGCCT GCTTTTTNGN ANNNNNNNNN N // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Suga, H., Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480 (7375): 57-62 (2011). PMID 22080957. [link to RRC of NBRP] |
---|---|
reference | Noura, M., Suppression of super-enhancer-driven TAL1 expression by KLF4 in T-cell acute lymphoblastic leukemia. Research Square https://doi.org/10.21203/rs.3.rs-3069900/v1 |
reference | Takata, N., Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9. Hum. Gene Ther. 27 (6): 436-450 (2016). PMID 26839115. |
user_report | Hada, M., Highly rigid H3.1/H3.2-H3K9me3 domains set a barrier for cell fate reprogramming in trophoblast stem cells. Genes Dev. 36 (1-2): 84-102 (2022). PMID 34992147. [link to RRC of NBRP] |
user_report | Matsui, Y., Chlorambucil-conjugated PI-polyamides (Chb-M'), a transcription inhibitor of RUNX family, has an anti-tumor activity against SHH-type medulloblastoma with p53 mutation. Biochem. Biophys. Res. Commun. 620: 150-157 (2022). PMID 35792512. [link to RRC of NBRP] |
user_report | Hattori, E.Y., A RUNX-targeted gene switch-off approach modulates the BIRC5/PIF1-p21 pathway and reduces glioblastoma growth in mice. Commun. Biol. 5 (1): 939 (2022). PMID 36085167. [link to RRC of NBRP] |
user_report | Nakatani, K., Inhibition of CDK4/6 and autophagy synergistically induces apoptosis in t(8;21) acute myeloid leukemia cells. Int. J. Hematol. 113 (2): 243-253 (2021). PMID 33068248. [link to RRC of NBRP] |
user_report | Kohrogi, K., LSD1 defines erythroleukemia metabolism by controlling the lineage-specific transcription factors GATA1 and C/EBPalpha. Blood Adv. 5 (9): 2305-2318 (2021). PMID 33929501. [link to RRC of NBRP] |
user_report | Morita, K., Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Cell 181 (3): 702-715.e20 (2020). PMID 32315619. [link to RRC of NBRP] |
user_report | Noura, M., Pivotal role of DPYSL2A in KLF4-mediated monocytic differentiation of acute myeloid leukemia cells. Sci. Rep. 10 (1): 20245 (2020). PMID 33219287. [link to RRC of NBRP] |
user_report | Morita, K., RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2 (5): 509-515 (2018). PMID 29500219. [link to RRC of NBRP] |
user_report | Mitsuda, Y., RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci. Rep. 8 (1): 6423 (2018). PMID 29686309. [link to RRC of NBRP] |
user_report | Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640. [link to RRC of NBRP] |
user_report | Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243. [link to RRC of NBRP] |
user_report | Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Adv. 1: 1440-1451 (2017). PMID 29296785. [link to RRC of NBRP] |
user_report | Hashizume, O., Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects. Sci. Rep. 5: 10434 (2015). PMID 26000717. [link to RRC of NBRP] |
user_report | Hirokawa, T., CBP-93872 inhibits NBS1-mediated ATR activation, abrogating maintenance of the DNA double-strand break-specific G2 checkpoint. Cancer Res. 74 (14): 3880-3889 (2014). PMID 24876101. [link to RRC of NBRP] |
2023.09.13
GNP_filter3_RDBDEP_html_230702.pl