Sequencing and PCR primers for primer information.
Backbone vector of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.
| Catalog number | RDB07915 |
|---|---|
| Resource name | CSII-EF-MCS-IRES2-hKO1 |
| Clone info. | Backbone vector of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
| Vector backbone | CSII-EF-MCS () |
| Selectable markers | Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells). |
| Originator | Miyoshi, Hiroyuki | |
| Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE. |
|---|---|
| Additional terms and conditions for distribution | RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding hKO1. |
| Ordering | Please visit Ordering instruction. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA [Word] Approval form [Word] by Dr. Atsushi Miyawaki |
| Remarks | ((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB07915 | CSII-EF-MCS-IRES2-hKO1 | DNA solution |
Electronic file
| Electronic file | Sequence RDB07915z.seq from Depositor |
|---|---|
| Electronic file | PDF (map, protocol and/or remarks) RDB07915.pdf from Depositor |
Featured content
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB12260_A3Kep1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: IRES-F | RDB12260_A3Kea.seq |
|---|---|
|
>07915_12260_A3Ke_IRES-F_G01_19.ab1 1 CCGTCCTTTA TGTGTTTAGT CGAGGTTAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG 61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACGG GGATCCCCAC CATGGTGAGC 121 GTGATCAAGC CCGAGATGAA GATGAAGTAC TTCATGGACG GCAGCGTGAA CGGCCACGAG 181 TTCACCGTGG AGGGCGAGGG CACCGGCAAG CCCTACGAGG GCCACCAGGA GATGACCCTG 241 AGGGTGACAA TGGCCAAGGG CGGCCCCATG CCCTTCAGCT TCGACCTGGT GAGCCACACC 301 TTCTGCTACG GCCACAGGCC CTTCACCAAG TACCCCGAGG AGATCCCCGA CTACTTCAAG 361 CAGGCCTTCC CCGAGGGCCT GAGCTGGGAG AGGAGCCTCC AGTTCGAGGA CGGCGGCTTC 421 GCCGCCGTGA GCGCCCACAT CAGCCTGAGG GGCAACTGCT TCGAGCACAA GAGCAAGTTC 481 GTGGGCGTGA ACTTCCCCGC CGACGGCCCC GTGATGCAGA ACCAGAGCAG CGACTGGGAG 541 CCCAGCACCG AGAAGATCAC CACCTGCGAC GGCGTGCTGA AGGGCGACGT GACCATGTAC 601 CTGAAGCTGG CCGGCGGCGG CAACCACAAG TGCCAGTTCA AGACCACCTA CAAGGCCGCC 661 AAGAAGATCC TGAAGATGCC CCAGAGCCAC TTCATCGGCC ACAGGCTGGT GAGGAAGACC 721 GAGGGCAACA TCACCGAGCT GGTGGAGGAC GCCGTGGCCC ACTGCTGACT GCAGGCTAGA 781 GTTAACATCG AGGGATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA 841 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA 901 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT 961 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT 1021 GCACTGTGTT TGCTGACGCA ACCCCCCCAC TGGGTTGGGG GCATTGCCAC CACCTGTCAG 1081 CTCCTTTTCT GGGACTTTCG CTTTCCCCCT CCTAATGGCA CGCCGACTCA ATCGACGCCT 1141 ACTTGCCGCT GCTGGAACAG GGGCCTTCG // |
|
| Primer: CMV-Forward | RDB12260_A3Keb.seq |
|
>07915_12260_A3Ke_CMV-Foward_D07_10.ab1 1 CGCTAACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC 121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA 181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG 241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC 301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG 361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG 421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT 481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC 541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC 601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA 661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT 721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA 781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA 841 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC 901 AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG 961 GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG 1021 GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCCAACA GCCATCTGAT 1081 TGCCACTTCA CAGGTCTGGG GGCCATCCAG CCAGCTTCCA GGCAAGAATT CCTTGGGGCC 1141 TGTTTG // |
|
Please visit Sequencing and PCR primers for primer information.
References
2019.04.18
GNP_filter3_RDBDEP_html_190418.pl