Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-MCS-IRES2-hKO1

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution	RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的提供条件	利用者は、hKO1蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書 [Word] 宮脇敦史博士による提供承諾書 [Word]
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16940_A9Cip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Sequence file: RDB16940_A9Cia.seq
>07915_16940_A9Ci_3_EF1a-C_F_A03_03_ABI24.ab1     1 CCCGGTTTTT TTGGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG    61 TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT TCTGCAGCGG   121 CCGCGGATCC GCCCCTCTCC CTCCCCCCCC CCTAACGTTA CTGGCCGAAG CCGCTTGGAA   181 TAAGGCCGGT GTGCGTTTGT CTATATGTTA TTTTCCACCA TATTGCCGTC TTTTGGCAAT   241 GTGAGGGCCC GGAAACCTGG CCCTGTCTTC TTGACGAGCA TTCCTAGGGG TCTTTCCCCT   301 CTCGCCAAAG GAATGCAAGG TCTGTTGAAT GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT   361 TCTTGAAGAC AAACAACGTC TGTAGCGACC CTTTGCAGGC AGCGGAACCC CCCACCTGGC   421 GACAGGTGCC TCTGCGGCCA AAAGCCACGT GTATAAGATA CACCTGCAAA GGCGGCACAA   481 CCCCAGTGCC ACGTTGTGAG TTGGATAGTT GTGGAAAGAG TCAAATGGCT CTCCTCAAGC   541 GTATTCAACA AGGGGCTGAA GGATGCCCAG AAGGTACCCC ATTGTATGGG ATCTGATCTG   601 GGGCCTCGGT ACACATGCTT TACATGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC   661 CCGAACCACG GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACGGGGAT   721 CCCCACCATG GTGAGCGTGA TCAAGCCCGA GATGAAGATG AAGTACTTCA TGGACGGCAG   781 CGTGAACGGC CACGAGTTCA CCGTGGAGGG CGAGGGCACC GGGCAAGCCC TACGAGGGCC   841 ACCAGGAGAT GACCCTGAGG GTGACAATGG GCCAAGGGCG GGCCCCATGC CCTTCAGCTT   901 CGACCTGTGA GCCACACCTT CTGCTACGGC CACAGCCTTC ACCAAGTACC CCGAGGAGAT   961 CCCCGGACTA CTTCAAGCAG TCAACCCGAT GCTGAGCTGG GAGAGGAGCC TCCAGTTCGA 1021 GACGGGCGGC CTTTC //
Primer: IRES-F Sequence file: RDB16940_A9Cib.seq
>07915_16940_A9Ci_3_IRES-F_F10_16_ABI24.ab1     1 GCGGCCTTTC ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA    61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC GGGGATCCCC ACCATGGTGA   121 GCGTGATCAA GCCCGAGATG AAGATGAAGT ACTTCATGGA CGGCAGCGTG AACGGCCACG   181 AGTTCACCGT GGAGGGCGAG GGCACCGGCA AGCCCTACGA GGGCCACCAG GAGATGACCC   241 TGAGGGTGAC AATGGCCAAG GGCGGCCCCA TGCCCTTCAG CTTCGACCTG GTGAGCCACA   301 CCTTCTGCTA CGGCCACAGG CCCTTCACCA AGTACCCCGA GGAGATCCCC GACTACTTCA   361 AGCAGGCCTT CCCCGAGGGC CTGAGCTGGG AGAGGAGCCT CCAGTTCGAG GACGGCGGCT   421 TCGCCGCCGT GAGCGCCCAC ATCAGCCTGA GGGGCAACTG CTTCGAGCAC AAGAGCAAGT   481 TCGTGGGCGT GAACTTCCCC GCCGACGGCC CCGTGATGCA GAACCAGAGC AGCGACTGGG   541 AGCCCAGCAC CGAGAAGATC ACCACCTGCG ACGGCGTGCT GAAGGGCGAC GTGACCATGT   601 ACCTGAAGCT GGCCGGCGGC GGCAACCACA AGTGCCAGTT CAAGACCACC TACAAGGCCG   661 CCAAGAAGAT CCTGAAGATG CCCCAGAGCC ACTTCATCGG CCACAGGCTG GTGAGGAAGA   721 CCGAGGGCAA CATCACCGAG CTGGTGGAGG ACGCCGTGGC CCACTGCTGA CTGCAGGCTA   781 GAGTTAACAT CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA   841 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT   901 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTTCTCC TCCTTTGTAT   961 AATCCCTGGT TGCTGTCTCT TTATGAAGGA GTTTGTGGCC CGTTGTCAGG CAACGTGGCG  1021 TGGGTGTTGC AACTGTGTTT GCTGACGCAA ACCCCCCCAC TTGATGGGGG CCATGCCACC  1081 AACCCTGGTC AGCTTCATTT CAGGAACCTT TCGCTTTCCC CCCGTCCCCT AATTTGCTAC //
Primer: BGH_rev2 Sequence file: RDB16940_A9Cic.seq
>07915_16940_A9Ci_3_BGH_rev2_D10_10_ABI24.ab1     1 CTTTCGGTCA GCATGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA   121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG   181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC   241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC   301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC   361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC   421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA   481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA   541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC   601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG   661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA   721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG   781 ACGGTATCGA TGCGGGGAGG CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG   841 AAGACGCGGA AGAGGCCGCA GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT   901 GAGGGCCGAA AGGGACGTAG CAGAAGGACG TCCCGCGCAG AAATCCAGGT GGGCAACACA   961 GGCGAGCAGC CCATGGGAAA GGACGTCAGC TTTCCCCGAA CAACACCACG AATTGTCAGT  1021 GCCCACAGCC CGAGCCCCTG TCAGCAGCGG GGCAAAGGCA GGCGGCGAAT GAAGTCGTCG  1081 TGTCATTAGG AGGGGGAAAG CGAAGGTCCT GGAAGAAGCT GAACAAGGTG GGGTGGCCAA 1141 ATGGC //
Primer: CMV-Forward Sequence file: RDB16940_A9Cid.seq
>07915_16940_A9Ci_3_CMV-Forward_C10_07_ABI24.ab1     1 CGTCACTCGC CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT   121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG   181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT   241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC   301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG   361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG   421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT   481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT   541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT   601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC   661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT   721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG GGCCGCTGAT   781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA   841 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGGTGC   901 AGAGAGAAAA AAAGAGCAGT GGGGAATAGG GAGCTTTGTT TCCTTGGGGT TTCTTTGGGA   961 GCAGCAGAAA GCACTATGGG GCGCAGCGTC AATGACGCTG ACGGTACAGC CCAGACATTA  1021 TTGTCTTGGG TATAAGGTGC AGCCAGCAGA TCAATTTGGC TGAGGGCTTT GAGCGCACAG 1081 CATCTGTTGC CACCTTCACG ACAGATTCTT TG //
Primer: EF1a-N_R Sequence file: RDB16940_A9Cie.seq
>07915_16940_A9Ci_3_EF1a-N_R_B03_06_ABI24.ab1     1 TGGGGGGGTA TGTGGGCGAT GTGCGCTCTG CCCACTGACG GGCACCGGAG CCTCACGAAG    61 CTTGAATTAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT   121 TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT   181 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT   241 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC   301 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA   361 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT   421 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT   481 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT   541 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT   601 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT   661 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA   721 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG   781 CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG   841 CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA CCGTCAGCGT   901 CATTGACGCT GCGCCCATAG TGCTTCCTGC TGCTCCCAAG AACCCAAGGG AACAAAAGCT   961 CCTAATTCCC ACTGCTTCTT TTTCTCCTCT GCACCACTCT TCTCTTTGCC CTTTGGTGGG  1021 GGTGCTAACT CCTAATTGGG TTCAATTTTT ACTTACCTTA TAATTATAAT AATTTCACGT 1081 TCCTCAATGG TCCTTCAGTA TCTCCTTCTC ACAGGTCCTG AAAGAAATCA AGCGCG //
Primer: SV40pro_F Sequence file: RDB16940_A9Cif.seq
>07915_16940_A9Ci_3_SV40pro_F_G10_19_ABI24.ab1     1 CACTGTCTGT ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA    61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC   121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA   181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG   241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG   301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT   361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC   421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG   481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA   541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT   601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA   661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG   721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA   781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA   841 ATCATGGTCA TAGCTGTTTT CCTGTGTGAA ATTGTTATCC CGCTCACAAT TCCACACAAA   901 CATACGAGCC GGGAAGCATA AAGTGTAAAG CCTGGGGGTG CCTAATGAGT GAGCTAACTC   961 ACATTAATTT GCGTTGCGCT CACTGCCCGC TTTTCCAGGT CGGGAACCTT GTCGTGCCCA  1021 GCTTGCATTT AATGAATCGG CCAACCGCCG CGGGAGAGGG CGGATTTGCG TATTGGGCGC 1081 TCTTCCGCTC CTCGCTTCAA CTGAACTCGC TTGCGCCTCG GTCGAATCGG GCCTGTCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl