Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.
Catalog number | RDB07915 |
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Resource name | CSII-EF-MCS-IRES2-hKO1 |
Clone info. | Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
Vector backbone | CSII-EF-MCS () |
Selectable markers | Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r. |
Depositor|Developer | Miyoshi, Hiroyuki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
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Additional terms and conditions for distribution | RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein. |
Ordering | Please visit Ordering instruction.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word ![]() Approval form [Word] ![]() |
Remarks | ((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Please obtain written approval of Dr. Atsushi Miyawaki. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB07915 | CSII-EF-MCS-IRES2-hKO1 | DNA solution |
Electronic file
Electronic file | Sequence RDB07915z.seq |
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Electronic file | Remarks, protocol and/or map (pdf) RDB07915.pdf |
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16940_A9Cip1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EF1a-C_F Sequence file: RDB16940_A9Cia.seq ![]() |
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>07915_16940_A9Ci_3_EF1a-C_F_A03_03_ABI24.ab1 1 CCCGGTTTTT TTGGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG 61 TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT TCTGCAGCGG 121 CCGCGGATCC GCCCCTCTCC CTCCCCCCCC CCTAACGTTA CTGGCCGAAG CCGCTTGGAA 181 TAAGGCCGGT GTGCGTTTGT CTATATGTTA TTTTCCACCA TATTGCCGTC TTTTGGCAAT 241 GTGAGGGCCC GGAAACCTGG CCCTGTCTTC TTGACGAGCA TTCCTAGGGG TCTTTCCCCT 301 CTCGCCAAAG GAATGCAAGG TCTGTTGAAT GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT 361 TCTTGAAGAC AAACAACGTC TGTAGCGACC CTTTGCAGGC AGCGGAACCC CCCACCTGGC 421 GACAGGTGCC TCTGCGGCCA AAAGCCACGT GTATAAGATA CACCTGCAAA GGCGGCACAA 481 CCCCAGTGCC ACGTTGTGAG TTGGATAGTT GTGGAAAGAG TCAAATGGCT CTCCTCAAGC 541 GTATTCAACA AGGGGCTGAA GGATGCCCAG AAGGTACCCC ATTGTATGGG ATCTGATCTG 601 GGGCCTCGGT ACACATGCTT TACATGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC 661 CCGAACCACG GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACGGGGAT 721 CCCCACCATG GTGAGCGTGA TCAAGCCCGA GATGAAGATG AAGTACTTCA TGGACGGCAG 781 CGTGAACGGC CACGAGTTCA CCGTGGAGGG CGAGGGCACC GGGCAAGCCC TACGAGGGCC 841 ACCAGGAGAT GACCCTGAGG GTGACAATGG GCCAAGGGCG GGCCCCATGC CCTTCAGCTT 901 CGACCTGTGA GCCACACCTT CTGCTACGGC CACAGCCTTC ACCAAGTACC CCGAGGAGAT 961 CCCCGGACTA CTTCAAGCAG TCAACCCGAT GCTGAGCTGG GAGAGGAGCC TCCAGTTCGA 1021 GACGGGCGGC CTTTC // |
Primer: IRES-F Sequence file: RDB16940_A9Cib.seq ![]() |
>07915_16940_A9Ci_3_IRES-F_F10_16_ABI24.ab1 1 GCGGCCTTTC ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA 61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC GGGGATCCCC ACCATGGTGA 121 GCGTGATCAA GCCCGAGATG AAGATGAAGT ACTTCATGGA CGGCAGCGTG AACGGCCACG 181 AGTTCACCGT GGAGGGCGAG GGCACCGGCA AGCCCTACGA GGGCCACCAG GAGATGACCC 241 TGAGGGTGAC AATGGCCAAG GGCGGCCCCA TGCCCTTCAG CTTCGACCTG GTGAGCCACA 301 CCTTCTGCTA CGGCCACAGG CCCTTCACCA AGTACCCCGA GGAGATCCCC GACTACTTCA 361 AGCAGGCCTT CCCCGAGGGC CTGAGCTGGG AGAGGAGCCT CCAGTTCGAG GACGGCGGCT 421 TCGCCGCCGT GAGCGCCCAC ATCAGCCTGA GGGGCAACTG CTTCGAGCAC AAGAGCAAGT 481 TCGTGGGCGT GAACTTCCCC GCCGACGGCC CCGTGATGCA GAACCAGAGC AGCGACTGGG 541 AGCCCAGCAC CGAGAAGATC ACCACCTGCG ACGGCGTGCT GAAGGGCGAC GTGACCATGT 601 ACCTGAAGCT GGCCGGCGGC GGCAACCACA AGTGCCAGTT CAAGACCACC TACAAGGCCG 661 CCAAGAAGAT CCTGAAGATG CCCCAGAGCC ACTTCATCGG CCACAGGCTG GTGAGGAAGA 721 CCGAGGGCAA CATCACCGAG CTGGTGGAGG ACGCCGTGGC CCACTGCTGA CTGCAGGCTA 781 GAGTTAACAT CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA 841 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 901 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTTCTCC TCCTTTGTAT 961 AATCCCTGGT TGCTGTCTCT TTATGAAGGA GTTTGTGGCC CGTTGTCAGG CAACGTGGCG 1021 TGGGTGTTGC AACTGTGTTT GCTGACGCAA ACCCCCCCAC TTGATGGGGG CCATGCCACC 1081 AACCCTGGTC AGCTTCATTT CAGGAACCTT TCGCTTTCCC CCCGTCCCCT AATTTGCTAC // |
Primer: BGH_rev2 Sequence file: RDB16940_A9Cic.seq ![]() |
>07915_16940_A9Ci_3_BGH_rev2_D10_10_ABI24.ab1 1 CTTTCGGTCA GCATGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA 121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG 181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC 241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC 301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC 361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC 421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA 481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA 541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC 601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG 661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA 721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG 781 ACGGTATCGA TGCGGGGAGG CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG 841 AAGACGCGGA AGAGGCCGCA GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT 901 GAGGGCCGAA AGGGACGTAG CAGAAGGACG TCCCGCGCAG AAATCCAGGT GGGCAACACA 961 GGCGAGCAGC CCATGGGAAA GGACGTCAGC TTTCCCCGAA CAACACCACG AATTGTCAGT 1021 GCCCACAGCC CGAGCCCCTG TCAGCAGCGG GGCAAAGGCA GGCGGCGAAT GAAGTCGTCG 1081 TGTCATTAGG AGGGGGAAAG CGAAGGTCCT GGAAGAAGCT GAACAAGGTG GGGTGGCCAA 1141 ATGGC // |
Primer: CMV-Forward Sequence file: RDB16940_A9Cid.seq ![]() |
>07915_16940_A9Ci_3_CMV-Forward_C10_07_ABI24.ab1 1 CGTCACTCGC CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT 121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG 181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT 241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC 301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG 361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG 421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT 481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT 541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT 601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC 661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT 721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG GGCCGCTGAT 781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA 841 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGGTGC 901 AGAGAGAAAA AAAGAGCAGT GGGGAATAGG GAGCTTTGTT TCCTTGGGGT TTCTTTGGGA 961 GCAGCAGAAA GCACTATGGG GCGCAGCGTC AATGACGCTG ACGGTACAGC CCAGACATTA 1021 TTGTCTTGGG TATAAGGTGC AGCCAGCAGA TCAATTTGGC TGAGGGCTTT GAGCGCACAG 1081 CATCTGTTGC CACCTTCACG ACAGATTCTT TG // |
Primer: EF1a-N_R Sequence file: RDB16940_A9Cie.seq ![]() |
>07915_16940_A9Ci_3_EF1a-N_R_B03_06_ABI24.ab1 1 TGGGGGGGTA TGTGGGCGAT GTGCGCTCTG CCCACTGACG GGCACCGGAG CCTCACGAAG 61 CTTGAATTAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT 121 TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT 181 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT 241 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC 301 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA 361 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT 421 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT 481 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT 541 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT 601 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT 661 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA 721 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG 781 CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG 841 CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA CCGTCAGCGT 901 CATTGACGCT GCGCCCATAG TGCTTCCTGC TGCTCCCAAG AACCCAAGGG AACAAAAGCT 961 CCTAATTCCC ACTGCTTCTT TTTCTCCTCT GCACCACTCT TCTCTTTGCC CTTTGGTGGG 1021 GGTGCTAACT CCTAATTGGG TTCAATTTTT ACTTACCTTA TAATTATAAT AATTTCACGT 1081 TCCTCAATGG TCCTTCAGTA TCTCCTTCTC ACAGGTCCTG AAAGAAATCA AGCGCG // |
Primer: SV40pro_F Sequence file: RDB16940_A9Cif.seq ![]() |
>07915_16940_A9Ci_3_SV40pro_F_G10_19_ABI24.ab1 1 CACTGTCTGT ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC 121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA 181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG 241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG 301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT 361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC 421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG 481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA 541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT 601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA 661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG 721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA 781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA 841 ATCATGGTCA TAGCTGTTTT CCTGTGTGAA ATTGTTATCC CGCTCACAAT TCCACACAAA 901 CATACGAGCC GGGAAGCATA AAGTGTAAAG CCTGGGGGTG CCTAATGAGT GAGCTAACTC 961 ACATTAATTT GCGTTGCGCT CACTGCCCGC TTTTCCAGGT CGGGAACCTT GTCGTGCCCA 1021 GCTTGCATTT AATGAATCGG CCAACCGCCG CGGGAGAGGG CGGATTTGCG TATTGGGCGC 1081 TCTTCCGCTC CTCGCTTCAA CTGAACTCGC TTGCGCCTCG GTCGAATCGG GCCTGTCG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2020.12.24
GNP_filter3_RDBDEP_html_201222.pl