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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-MCS-IRES2-hKO1

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.

Catalog number RDB07915
Resource name CSII-EF-MCS-IRES2-hKO1
Clone info. Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS ()
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering Please visit Ordering instruction.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07915 CSII-EF-MCS-IRES2-hKO1 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB07915z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB07915.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16940_A9Cip1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB16940_A9Cia.seq check
>07915_16940_A9Ci_3_EF1a-C_F_A03_03_ABI24.ab1
    1 CCCGGTTTTT TTGGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG
   61 TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT TCTGCAGCGG
  121 CCGCGGATCC GCCCCTCTCC CTCCCCCCCC CCTAACGTTA CTGGCCGAAG CCGCTTGGAA
  181 TAAGGCCGGT GTGCGTTTGT CTATATGTTA TTTTCCACCA TATTGCCGTC TTTTGGCAAT
  241 GTGAGGGCCC GGAAACCTGG CCCTGTCTTC TTGACGAGCA TTCCTAGGGG TCTTTCCCCT
  301 CTCGCCAAAG GAATGCAAGG TCTGTTGAAT GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT
  361 TCTTGAAGAC AAACAACGTC TGTAGCGACC CTTTGCAGGC AGCGGAACCC CCCACCTGGC
  421 GACAGGTGCC TCTGCGGCCA AAAGCCACGT GTATAAGATA CACCTGCAAA GGCGGCACAA
  481 CCCCAGTGCC ACGTTGTGAG TTGGATAGTT GTGGAAAGAG TCAAATGGCT CTCCTCAAGC
  541 GTATTCAACA AGGGGCTGAA GGATGCCCAG AAGGTACCCC ATTGTATGGG ATCTGATCTG
  601 GGGCCTCGGT ACACATGCTT TACATGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC
  661 CCGAACCACG GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACGGGGAT
  721 CCCCACCATG GTGAGCGTGA TCAAGCCCGA GATGAAGATG AAGTACTTCA TGGACGGCAG
  781 CGTGAACGGC CACGAGTTCA CCGTGGAGGG CGAGGGCACC GGGCAAGCCC TACGAGGGCC
  841 ACCAGGAGAT GACCCTGAGG GTGACAATGG GCCAAGGGCG GGCCCCATGC CCTTCAGCTT
  901 CGACCTGTGA GCCACACCTT CTGCTACGGC CACAGCCTTC ACCAAGTACC CCGAGGAGAT
  961 CCCCGGACTA CTTCAAGCAG TCAACCCGAT GCTGAGCTGG GAGAGGAGCC TCCAGTTCGA
1021 GACGGGCGGC CTTTC
//
Primer: IRES-F
Sequence file: RDB16940_A9Cib.seq check
>07915_16940_A9Ci_3_IRES-F_F10_16_ABI24.ab1
    1 GCGGCCTTTC ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA
   61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC GGGGATCCCC ACCATGGTGA
  121 GCGTGATCAA GCCCGAGATG AAGATGAAGT ACTTCATGGA CGGCAGCGTG AACGGCCACG
  181 AGTTCACCGT GGAGGGCGAG GGCACCGGCA AGCCCTACGA GGGCCACCAG GAGATGACCC
  241 TGAGGGTGAC AATGGCCAAG GGCGGCCCCA TGCCCTTCAG CTTCGACCTG GTGAGCCACA
  301 CCTTCTGCTA CGGCCACAGG CCCTTCACCA AGTACCCCGA GGAGATCCCC GACTACTTCA
  361 AGCAGGCCTT CCCCGAGGGC CTGAGCTGGG AGAGGAGCCT CCAGTTCGAG GACGGCGGCT
  421 TCGCCGCCGT GAGCGCCCAC ATCAGCCTGA GGGGCAACTG CTTCGAGCAC AAGAGCAAGT
  481 TCGTGGGCGT GAACTTCCCC GCCGACGGCC CCGTGATGCA GAACCAGAGC AGCGACTGGG
  541 AGCCCAGCAC CGAGAAGATC ACCACCTGCG ACGGCGTGCT GAAGGGCGAC GTGACCATGT
  601 ACCTGAAGCT GGCCGGCGGC GGCAACCACA AGTGCCAGTT CAAGACCACC TACAAGGCCG
  661 CCAAGAAGAT CCTGAAGATG CCCCAGAGCC ACTTCATCGG CCACAGGCTG GTGAGGAAGA
  721 CCGAGGGCAA CATCACCGAG CTGGTGGAGG ACGCCGTGGC CCACTGCTGA CTGCAGGCTA
  781 GAGTTAACAT CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA
  841 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT
  901 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTTCTCC TCCTTTGTAT
  961 AATCCCTGGT TGCTGTCTCT TTATGAAGGA GTTTGTGGCC CGTTGTCAGG CAACGTGGCG
 1021 TGGGTGTTGC AACTGTGTTT GCTGACGCAA ACCCCCCCAC TTGATGGGGG CCATGCCACC
 1081 AACCCTGGTC AGCTTCATTT CAGGAACCTT TCGCTTTCCC CCCGTCCCCT AATTTGCTAC
//
Primer: BGH_rev2
Sequence file: RDB16940_A9Cic.seq check
>07915_16940_A9Ci_3_BGH_rev2_D10_10_ABI24.ab1
    1 CTTTCGGTCA GCATGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
  121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
  181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
  241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC
  301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC
  361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC
  421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA
  481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA
  541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC
  601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG
  661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA
  721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG
  781 ACGGTATCGA TGCGGGGAGG CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG
  841 AAGACGCGGA AGAGGCCGCA GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT
  901 GAGGGCCGAA AGGGACGTAG CAGAAGGACG TCCCGCGCAG AAATCCAGGT GGGCAACACA
  961 GGCGAGCAGC CCATGGGAAA GGACGTCAGC TTTCCCCGAA CAACACCACG AATTGTCAGT
 1021 GCCCACAGCC CGAGCCCCTG TCAGCAGCGG GGCAAAGGCA GGCGGCGAAT GAAGTCGTCG
 1081 TGTCATTAGG AGGGGGAAAG CGAAGGTCCT GGAAGAAGCT GAACAAGGTG GGGTGGCCAA
1141 ATGGC
//
Primer: CMV-Forward
Sequence file: RDB16940_A9Cid.seq check
>07915_16940_A9Ci_3_CMV-Forward_C10_07_ABI24.ab1
    1 CGTCACTCGC CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
  121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
  181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
  241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
  301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
  361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
  421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
  481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
  541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
  601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
  661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
  721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG GGCCGCTGAT
  781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA
  841 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGGTGC
  901 AGAGAGAAAA AAAGAGCAGT GGGGAATAGG GAGCTTTGTT TCCTTGGGGT TTCTTTGGGA
  961 GCAGCAGAAA GCACTATGGG GCGCAGCGTC AATGACGCTG ACGGTACAGC CCAGACATTA
 1021 TTGTCTTGGG TATAAGGTGC AGCCAGCAGA TCAATTTGGC TGAGGGCTTT GAGCGCACAG
1081 CATCTGTTGC CACCTTCACG ACAGATTCTT TG
//
Primer: EF1a-N_R
Sequence file: RDB16940_A9Cie.seq check
>07915_16940_A9Ci_3_EF1a-N_R_B03_06_ABI24.ab1
    1 TGGGGGGGTA TGTGGGCGAT GTGCGCTCTG CCCACTGACG GGCACCGGAG CCTCACGAAG
   61 CTTGAATTAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT
  121 TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT
  181 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT
  241 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC
  301 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA
  361 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT
  421 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT
  481 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT
  541 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT
  601 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT
  661 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA
  721 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG
  781 CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG
  841 CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA CCGTCAGCGT
  901 CATTGACGCT GCGCCCATAG TGCTTCCTGC TGCTCCCAAG AACCCAAGGG AACAAAAGCT
  961 CCTAATTCCC ACTGCTTCTT TTTCTCCTCT GCACCACTCT TCTCTTTGCC CTTTGGTGGG
 1021 GGTGCTAACT CCTAATTGGG TTCAATTTTT ACTTACCTTA TAATTATAAT AATTTCACGT
1081 TCCTCAATGG TCCTTCAGTA TCTCCTTCTC ACAGGTCCTG AAAGAAATCA AGCGCG
//
Primer: SV40pro_F
Sequence file: RDB16940_A9Cif.seq check
>07915_16940_A9Ci_3_SV40pro_F_G10_19_ABI24.ab1
    1 CACTGTCTGT ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
  121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
  181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
  241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
  301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
  361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
  421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
  481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
  541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
  661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
  841 ATCATGGTCA TAGCTGTTTT CCTGTGTGAA ATTGTTATCC CGCTCACAAT TCCACACAAA
  901 CATACGAGCC GGGAAGCATA AAGTGTAAAG CCTGGGGGTG CCTAATGAGT GAGCTAACTC
  961 ACATTAATTT GCGTTGCGCT CACTGCCCGC TTTTCCAGGT CGGGAACCTT GTCGTGCCCA
 1021 GCTTGCATTT AATGAATCGG CCAACCGCCG CGGGAGAGGG CGGATTTGCG TATTGGGCGC
1081 TCTTCCGCTC CTCGCTTCAA CTGAACTCGC TTGCGCCTCG GTCGAATCGG GCCTGTCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Morita, K., Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Cell 181 (3): 702-715.e20 (2020). PMID 32315619.
user report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
user report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
user report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Advances 1: 1440-1451 (2017). PMID 29296785.
user report Lee, J., Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation. Cell Stem. Cell, 5, 76-86 (2009). PMID 19570516.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl