Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-MCS-IRES2-hKO1

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.

Sequence (full)	RDB07915zzk02.seq provided by the depositor
Remarks, protocol and/or map (pdf)	RDB07915.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 付加的提供条件： 利用者は、hKO1蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 宮脇敦史博士による提供承諾書 [Word]
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16940_A9Cip1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro, IRES2 Sequence file: RDB16940_A9Cig.seq
>07915_16940_A9Ci_3_EF1a-C_F_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNTTTTGAN TTTGGNNCTT GGTTCNTTCT CNAGCCTCAG ACAGTGGTTC    61 AAAGTTTTTT TCTTCCNTTT CAGGTGTCGT GAACACGCTA CCGGTCTCGA GAATTCTGCA   121 GCGGCCGCGG ATCCGCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT   181 GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG   241 CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC   301 CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA   361 AGCTTCTTGA AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC   421 TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC   481 ACAACCCCAG TGCCACGTTG TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC   541 AAGCGTATTC AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA   601 TCTGGGGCCT CGGTNCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG   661 CCCCCCGAAC CACNGGGANG TGGTTTTCCT TTNAAAAACA CGATGNTAAT ATGGCCACGG   721 GGNTNCCCAC CATGGTGAGC GTGNTCAAGC CCGANATGAA GATGAAGTAC TTCNTNGNNG   781 GNANCGTNNN CGGNNNCNAN TTNNNCGTGG ANGNNGAGGG CACCNGNNNA NCCCTNCNAN   841 GGNNNCCNNN ANNTGANCCT NANGNNNANA NTNGNNNNAG GGCNGNCCCN NNCCNTTNNN 901 CNNNNNNNTN NNNNNNCNCN NNTTNNNNTN NNNNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE, hKO, IRES2 Sequence file: RDB16940_A9Cih.seq
>07915_16940_A9Ci_3_PRE_rev_B10_04_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNNN NNNNCNNCAT AGTTAAGAAN ACCAGTCAAT CTTTCNCAAA    61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGCCTG   121 CAGTCAGCAG TGGGCCACGG CGTCCTCCAC CAGCTCGGTG ATGTTGCCCT CGGTCTTCCT   181 CACCAGCCTG TGGCCGATGA AGTGGCTCTG GGGCATCTTC AGGATCTTCT TGGCGGCCTT   241 GTAGGTGGTC TTGAACTGGC ACTTGTGGTT GCCGCCGCCG GCCAGCTTCA GGTACATGGT   301 CACGTCGCCC TTCAGCACGC CGTCGCAGGT GGTGATCTTC TCGGTGCTGG GCTCCCAGTC   361 GCTGCTCTGG TTCTGCATCA CGGGGCCGTC GGCGGGGAAG TTCACGCCCA CGAACTTGCT   421 CTTGTGCTCG AAGCAGTTGC CCCTCAGGCT GATGTGGGCG CTCACGGCGG CGAAGCCGCC   481 GTCCTCGAAC TGGAGGCTCC TCTCCCAGCT CAGGCCCTCG GGGAAGGCCT GCTTGAAGTA   541 GTCGGGGATC TCCTCGGGGT ACTTGGTGAA GGGCCTGTGG CCGTAGCAGA AGGTGTGGCT   601 CACCAGGTCG AAGCTGAAGG GCATGGGGCC GCCCTTGGCC ATTGTCACCC TCAGGGTCAT   661 CTCCTGGTGG CCCTCGTAGG GCTTGCCGGT GCCCTCGCCC TCCACGGTGA ACTCGTGGCC   721 GTTCACGCTG CCGTCCATGA AGTACTTCAT CTTCATCTCG GGCTTGATCA CGCTCACCAT   781 GGTGGGGATC CCCGTGGCCA TATTATCATC GTGTTTTTCA AANGAAAACC ACGTCCCCNN   841 GGTTCGGGGG GGNCTANANG TTTTTTTAAC CTCNANTAAN CACATGTAAA GCATGTGTAN   901 CNANGCCCCA NNTCANATNC NNTACAANGG GGGTNNNNTC TGGNNATCCT TCANCCCNNT 961 NNNTGAATAN NCNTNNANNA NNNCNNTTTG NNNNTTNNNN NNNNNN //
Primer: IRES-F (Pr0036) Region: IRES2, hKO 5' Sequence file: RDB16940_A9Cii.seq
>07915_16940_A9Ci_3_IRES-F_F10_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNTNNG TCGANGTTNN NAAACGTCTA GGCCCCCCGA ACCACGGGGN    61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC GGGGATCCCC ACCATGGTGA   121 GCGTGATCAA GCCCGAGATG AAGATGAAGT ACTTCATGGA CGGCAGCGTG AACGGCCACG   181 AGTTCACCGT GGAGGGCGAG GGCACCGGCA AGCCCTACGA GGGCCACCAG GAGATGACCC   241 TGAGGGTGAC AATGGCCAAG GGCGGCCCCA TGCCCTTCAG CTTCGACCTG GTGAGCCACA   301 CCTTCTGCTA CGGCCACAGG CCCTTCACCA AGTACCCCGA GGAGATCCCC GACTACTTCA   361 AGCAGGCCTT CCCCGAGGGC CTGAGCTGGG AGAGGAGCCT CCAGTTCGAG GACGGCGGCT   421 TCGCCGCCGT GAGCGCCCAC ATCAGCCTGA GGGGCAACTG CTTCGAGCAC AAGAGCAAGT   481 TCGTGGGCGT GAACTTCCCC GCCGACGGCC CCGTGATGCA GAACCAGAGC AGCGACTGGG   541 AGCCCAGCAC CGAGAAGATC ACCACCTGCG ACGGCGTGCT GAAGGGCGAC GTGACCATGT   601 ACCTGAAGCT GGCCGGCGGC GGCAACCACA AGTGCCAGTT CAAGACCACC TACAAGGCCG   661 CCAAGAAGAT CCTGAAGANG CCCCAGAGCC ACTTCATCGG CCACAGGCTG GTGAGGAAGA   721 NCGANGGCAA CATCACCGAN CTGGTGGAGG ACGCCGTGGC CCACTGCTGA CTGCAGGCTA   781 GAGTTAACAT CGAGGGATCN AGCTTATCGA TNATCAACCT CTGGNNTACN AAATTTGTGA   841 AAGANTGACT GGNATTCTNA ACTATGTTGC TCCNTTTACG CTATGNNGNN ACGCTGCTTN   901 NANGNCTTTG NATCATGCTA TNGNNNCCCG TANNNNTTNN ATTTTNNNNN NNCNTNNNAN 961 ANNNCNNNNN NNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5' LTR, HIV-1 psi Sequence file: RDB16940_A9Cij.seq
>07915_16940_A9Ci_3_CMV-Forward_C10_07_ABI24.ab1     1 NNNNNNNNNN NNNNTTGNNG CNNTGGGCGG TAGGCGTGTN CGGTGGGAGG TCTATATAAG    61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT   121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG   181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT   241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC   301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG   361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG   421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT   481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT   541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT   601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC   661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT   721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG GGCCGCTGAT   781 CTTCAGANCT GGNNGANGAN ATATGAGGGA NAATTGGAGA AGTGAATTAT ATAAATATNA   841 AGTAGTAAAA ATTGAACCNT TANGANNAGC NCCCACNNNG GNNAAGANAA NNNNNGNNGN   901 ANANNNNAAA AAAGANCANN NGGNANTNNG NNNNNTNNNT NNNNNNGGNN TNNNTNNGNN 961 NNNNNNNN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro, cPPT_CTS Sequence file: RDB16940_A9Cik.seq
>07915_16940_A9Ci_3_EF1a-N_R_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNGGNGNN GTGCGCTCTG CCCNCTGNCG GGCACCGGAN CCTCACGAAG    61 CTTGAATTAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT   121 TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT   181 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT   241 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC   301 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA   361 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT   421 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT   481 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT   541 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT   601 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT   661 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCNCN   721 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG   781 CTGCTTGATG CCCCAGACTG TGANTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAN   841 NAANNNGNTC TGCTGCTGCA CTATACCAGA NAATAATTGT CTGGNCTGTA CCNNCAGCGN   901 CNTTNACNCN NCGCCCATAN NGCTTNCNGN NNCTNNNNNN ANCCCAANNN NNNAAANNTN 961 NNNNTNCCCN CTGCNNNTTT NNNNNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA, 3' LTR Sequence file: RDB16940_A9Cil.seq
>07915_16940_A9Ci_3_BGH_rev2_D10_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG GGGNNGGNNN NNNACNGATG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA   121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG   181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC   241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC   301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC   361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC   421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA   481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA   541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC   601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG   661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA   721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG   781 ACNGTATCGA TGCGGGGANG CGGCCCNAAG GGAGATCCGA CTCGTCTGAN GGCGANNNNG   841 AAGANGCGGA AGANGNNGCA NANCCNNNNN CNNNNCNCNG GAAGGAAGGN CCGCTGGATT   901 GANGGNCNNA NNGNANNNNN CNNAANGNNN TCCCGCNCAN AANNNNNNGN NGNNNNNNCN 961 NNNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori, EM7 pro, Zeo, SV40 pA Sequence file: RDB16940_A9Cim.seq
>07915_16940_A9Ci_3_SV40pro_F_G10_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAN NNGTGNNGNN GNTTTTTTGG ANGCCTAGGC TTTTGCAAAA    61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC   121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA   181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG   241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG   301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT   361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC   421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG   481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA   541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT   601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA   661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG   721 CATCACAAAT TTCACNAATA AAGCATTTTT TTCACTGCAT TCTANNNGTG GTTTGTCCNA   781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCNACC TCTAGCTAGA GCTTGNCGTA   841 NTCATGGTCA TAGCNGNTTN CNTGTGTGAA ANTGTTATNC NNCTCACAAT TNCNCACNAN   901 CATACGANCC NGGANNCATA AAGTGTAAAN CCNGGGGGTG NCNNANNNNT GNNCTANNNN 961 NCNTTNANTN GNNTTGCNCN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl