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CSII-EF-MCS-IRES2-hKO1 (#RDB07915)

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.

Clone info. Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-EF-MCS (RDB04378) ()
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence (full) RDB07915zzk02.seq provided by the depositor
Remarks, protocol and/or map (pdf) RDB07915.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07915 CSII-EF-MCS-IRES2-hKO1 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-EF-MCS-IRES2-hKO1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07915).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16940_A9Cip1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro, IRES2
Sequence file: RDB16940_A9Cig.seq check
>07915_16940_A9Ci_3_EF1a-C_F_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNTTTTGAN TTTGGNNCTT GGTTCNTTCT CNAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCNTTT CAGGTGTCGT GAACACGCTA CCGGTCTCGA GAATTCTGCA
  121 GCGGCCGCGG ATCCGCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT
  181 GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG
  241 CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC
  301 CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA
  361 AGCTTCTTGA AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC
  421 TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC
  481 ACAACCCCAG TGCCACGTTG TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC
  541 AAGCGTATTC AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA
  601 TCTGGGGCCT CGGTNCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG
  661 CCCCCCGAAC CACNGGGANG TGGTTTTCCT TTNAAAAACA CGATGNTAAT ATGGCCACGG
  721 GGNTNCCCAC CATGGTGAGC GTGNTCAAGC CCGANATGAA GATGAAGTAC TTCNTNGNNG
  781 GNANCGTNNN CGGNNNCNAN TTNNNCGTGG ANGNNGAGGG CACCNGNNNA NCCCTNCNAN
  841 GGNNNCCNNN ANNTGANCCT NANGNNNANA NTNGNNNNAG GGCNGNCCCN NNCCNTTNNN
901 CNNNNNNNTN NNNNNNCNCN NNTTNNNNTN NNNNNNNNN
//
Primer: PRE_rev (Pr0250)
Region: PRE, hKO, IRES2
Sequence file: RDB16940_A9Cih.seq check
>07915_16940_A9Ci_3_PRE_rev_B10_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNNN NNNNCNNCAT AGTTAAGAAN ACCAGTCAAT CTTTCNCAAA
   61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGCCTG
  121 CAGTCAGCAG TGGGCCACGG CGTCCTCCAC CAGCTCGGTG ATGTTGCCCT CGGTCTTCCT
  181 CACCAGCCTG TGGCCGATGA AGTGGCTCTG GGGCATCTTC AGGATCTTCT TGGCGGCCTT
  241 GTAGGTGGTC TTGAACTGGC ACTTGTGGTT GCCGCCGCCG GCCAGCTTCA GGTACATGGT
  301 CACGTCGCCC TTCAGCACGC CGTCGCAGGT GGTGATCTTC TCGGTGCTGG GCTCCCAGTC
  361 GCTGCTCTGG TTCTGCATCA CGGGGCCGTC GGCGGGGAAG TTCACGCCCA CGAACTTGCT
  421 CTTGTGCTCG AAGCAGTTGC CCCTCAGGCT GATGTGGGCG CTCACGGCGG CGAAGCCGCC
  481 GTCCTCGAAC TGGAGGCTCC TCTCCCAGCT CAGGCCCTCG GGGAAGGCCT GCTTGAAGTA
  541 GTCGGGGATC TCCTCGGGGT ACTTGGTGAA GGGCCTGTGG CCGTAGCAGA AGGTGTGGCT
  601 CACCAGGTCG AAGCTGAAGG GCATGGGGCC GCCCTTGGCC ATTGTCACCC TCAGGGTCAT
  661 CTCCTGGTGG CCCTCGTAGG GCTTGCCGGT GCCCTCGCCC TCCACGGTGA ACTCGTGGCC
  721 GTTCACGCTG CCGTCCATGA AGTACTTCAT CTTCATCTCG GGCTTGATCA CGCTCACCAT
  781 GGTGGGGATC CCCGTGGCCA TATTATCATC GTGTTTTTCA AANGAAAACC ACGTCCCCNN
  841 GGTTCGGGGG GGNCTANANG TTTTTTTAAC CTCNANTAAN CACATGTAAA GCATGTGTAN
  901 CNANGCCCCA NNTCANATNC NNTACAANGG GGGTNNNNTC TGGNNATCCT TCANCCCNNT
961 NNNTGAATAN NCNTNNANNA NNNCNNTTTG NNNNTTNNNN NNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES2, hKO 5'
Sequence file: RDB16940_A9Cii.seq check
>07915_16940_A9Ci_3_IRES-F_F10_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNNG TCGANGTTNN NAAACGTCTA GGCCCCCCGA ACCACGGGGN
   61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC GGGGATCCCC ACCATGGTGA
  121 GCGTGATCAA GCCCGAGATG AAGATGAAGT ACTTCATGGA CGGCAGCGTG AACGGCCACG
  181 AGTTCACCGT GGAGGGCGAG GGCACCGGCA AGCCCTACGA GGGCCACCAG GAGATGACCC
  241 TGAGGGTGAC AATGGCCAAG GGCGGCCCCA TGCCCTTCAG CTTCGACCTG GTGAGCCACA
  301 CCTTCTGCTA CGGCCACAGG CCCTTCACCA AGTACCCCGA GGAGATCCCC GACTACTTCA
  361 AGCAGGCCTT CCCCGAGGGC CTGAGCTGGG AGAGGAGCCT CCAGTTCGAG GACGGCGGCT
  421 TCGCCGCCGT GAGCGCCCAC ATCAGCCTGA GGGGCAACTG CTTCGAGCAC AAGAGCAAGT
  481 TCGTGGGCGT GAACTTCCCC GCCGACGGCC CCGTGATGCA GAACCAGAGC AGCGACTGGG
  541 AGCCCAGCAC CGAGAAGATC ACCACCTGCG ACGGCGTGCT GAAGGGCGAC GTGACCATGT
  601 ACCTGAAGCT GGCCGGCGGC GGCAACCACA AGTGCCAGTT CAAGACCACC TACAAGGCCG
  661 CCAAGAAGAT CCTGAAGANG CCCCAGAGCC ACTTCATCGG CCACAGGCTG GTGAGGAAGA
  721 NCGANGGCAA CATCACCGAN CTGGTGGAGG ACGCCGTGGC CCACTGCTGA CTGCAGGCTA
  781 GAGTTAACAT CGAGGGATCN AGCTTATCGA TNATCAACCT CTGGNNTACN AAATTTGTGA
  841 AAGANTGACT GGNATTCTNA ACTATGTTGC TCCNTTTACG CTATGNNGNN ACGCTGCTTN
  901 NANGNCTTTG NATCATGCTA TNGNNNCCCG TANNNNTTNN ATTTTNNNNN NNCNTNNNAN
961 ANNNCNNNNN NNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, 5' LTR, HIV-1 psi
Sequence file: RDB16940_A9Cij.seq check
>07915_16940_A9Ci_3_CMV-Forward_C10_07_ABI24.ab1
    1 NNNNNNNNNN NNNNTTGNNG CNNTGGGCGG TAGGCGTGTN CGGTGGGAGG TCTATATAAG
   61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
  121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
  181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
  241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
  301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
  361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
  421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
  481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
  541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
  601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
  661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
  721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG GGCCGCTGAT
  781 CTTCAGANCT GGNNGANGAN ATATGAGGGA NAATTGGAGA AGTGAATTAT ATAAATATNA
  841 AGTAGTAAAA ATTGAACCNT TANGANNAGC NCCCACNNNG GNNAAGANAA NNNNNGNNGN
  901 ANANNNNAAA AAAGANCANN NGGNANTNNG NNNNNTNNNT NNNNNNGGNN TNNNTNNGNN
961 NNNNNNNN
//
Primer: EF1a-N_R (Pr0024)
Region: EF-1 alpha pro, cPPT_CTS
Sequence file: RDB16940_A9Cik.seq check
>07915_16940_A9Ci_3_EF1a-N_R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNGGNGNN GTGCGCTCTG CCCNCTGNCG GGCACCGGAN CCTCACGAAG
   61 CTTGAATTAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT
  121 TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT
  181 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT
  241 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC
  301 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA
  361 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT
  421 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT
  481 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT
  541 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT
  601 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT
  661 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCNCN
  721 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG
  781 CTGCTTGATG CCCCAGACTG TGANTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAN
  841 NAANNNGNTC TGCTGCTGCA CTATACCAGA NAATAATTGT CTGGNCTGTA CCNNCAGCGN
  901 CNTTNACNCN NCGCCCATAN NGCTTNCNGN NNCTNNNNNN ANCCCAANNN NNNAAANNTN
961 NNNNTNCCCN CTGCNNNTTT NNNNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA, 3' LTR
Sequence file: RDB16940_A9Cil.seq check
>07915_16940_A9Ci_3_BGH_rev2_D10_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG GGGNNGGNNN NNNACNGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
  121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
  181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
  241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC
  301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC
  361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC
  421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA
  481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA
  541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC
  601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG
  661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA
  721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG
  781 ACNGTATCGA TGCGGGGANG CGGCCCNAAG GGAGATCCGA CTCGTCTGAN GGCGANNNNG
  841 AAGANGCGGA AGANGNNGCA NANCCNNNNN CNNNNCNCNG GAAGGAAGGN CCGCTGGATT
  901 GANGGNCNNA NNGNANNNNN CNNAANGNNN TCCCGCNCAN AANNNNNNGN NGNNNNNNCN
961 NNNNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori, EM7 pro, Zeo, SV40 pA
Sequence file: RDB16940_A9Cim.seq check
>07915_16940_A9Ci_3_SV40pro_F_G10_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN NNGTGNNGNN GNTTTTTTGG ANGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
  121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
  181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
  241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
  301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
  361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
  421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
  481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
  541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
  661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  721 CATCACAAAT TTCACNAATA AAGCATTTTT TTCACTGCAT TCTANNNGTG GTTTGTCCNA
  781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCNACC TCTAGCTAGA GCTTGNCGTA
  841 NTCATGGTCA TAGCNGNTTN CNTGTGTGAA ANTGTTATNC NNCTCACAAT TNCNCACNAN
  901 CATACGANCC NGGANNCATA AAGTGTAAAN CCNGGGGGTG NCNNANNNNT GNNCTANNNN
961 NCNTTNANTN GNNTTGCNCN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Nakao, F., Targeting a mitochondrial E3 ubiquitin ligase complex to overcome AML cell-intrinsic Venetoclax resistance. Leukemia 37 (5): 1028-1038 (2023). PMID 36973350. [link to RRC of NBRP]
user_report Takeuchi, K., Incorporation of human iPSC-derived stromal cells creates a pancreatic cancer organoid with heterogeneous cancer-associated fibroblasts. Cell Rep. 42 (11): 113420 (2023). PMID 37955987. [link to RRC of NBRP]
user_report Morita, K., Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Cell 181 (3): 702-715.e20 (2020). PMID 32315619. [link to RRC of NBRP]
user_report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640. [link to RRC of NBRP]
user_report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243. [link to RRC of NBRP]
user_report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Adv. 1: 1440-1451 (2017). PMID 29296785. [link to RRC of NBRP]
user_report Lee, J., Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation. Cell Stem. Cell, 5, 76-86 (2009). PMID 19570516. [link to RRC of NBRP]