Resource data sheet
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CSII-EF-MCS-IRES2-hKO1

Backbone vector of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.

Catalog number RDB07915
Resource name CSII-EF-MCS-IRES2-hKO1
Clone info. Backbone vector of lentivirus construct driven by EF-1 alpha promoter with hKO1 fluorescent protein marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS ()
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding hKO1.
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Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
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RDB07915 CSII-EF-MCS-IRES2-hKO1 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07915z.seq from Depositor
Electronic file PDF RDB07915.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12260_A3Kep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-F RDB12260_A3Kea.seq
>07915_12260_A3Ke_IRES-F_G01_19.ab1
    1 CCGTCCTTTA TGTGTTTAGT CGAGGTTAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
   61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACGG GGATCCCCAC CATGGTGAGC
  121 GTGATCAAGC CCGAGATGAA GATGAAGTAC TTCATGGACG GCAGCGTGAA CGGCCACGAG
  181 TTCACCGTGG AGGGCGAGGG CACCGGCAAG CCCTACGAGG GCCACCAGGA GATGACCCTG
  241 AGGGTGACAA TGGCCAAGGG CGGCCCCATG CCCTTCAGCT TCGACCTGGT GAGCCACACC
  301 TTCTGCTACG GCCACAGGCC CTTCACCAAG TACCCCGAGG AGATCCCCGA CTACTTCAAG
  361 CAGGCCTTCC CCGAGGGCCT GAGCTGGGAG AGGAGCCTCC AGTTCGAGGA CGGCGGCTTC
  421 GCCGCCGTGA GCGCCCACAT CAGCCTGAGG GGCAACTGCT TCGAGCACAA GAGCAAGTTC
  481 GTGGGCGTGA ACTTCCCCGC CGACGGCCCC GTGATGCAGA ACCAGAGCAG CGACTGGGAG
  541 CCCAGCACCG AGAAGATCAC CACCTGCGAC GGCGTGCTGA AGGGCGACGT GACCATGTAC
  601 CTGAAGCTGG CCGGCGGCGG CAACCACAAG TGCCAGTTCA AGACCACCTA CAAGGCCGCC
  661 AAGAAGATCC TGAAGATGCC CCAGAGCCAC TTCATCGGCC ACAGGCTGGT GAGGAAGACC
  721 GAGGGCAACA TCACCGAGCT GGTGGAGGAC GCCGTGGCCC ACTGCTGACT GCAGGCTAGA
  781 GTTAACATCG AGGGATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA
  841 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA
  901 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT
  961 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT
 1021 GCACTGTGTT TGCTGACGCA ACCCCCCCAC TGGGTTGGGG GCATTGCCAC CACCTGTCAG
 1081 CTCCTTTTCT GGGACTTTCG CTTTCCCCCT CCTAATGGCA CGCCGACTCA ATCGACGCCT
1141 ACTTGCCGCT GCTGGAACAG GGGCCTTCG
//
Primer: CMV-Forward RDB12260_A3Keb.seq
>07915_12260_A3Ke_CMV-Foward_D07_10.ab1
    1 CGCTAACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA
  781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA
  841 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC
  901 AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG
  961 GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG
 1021 GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCCAACA GCCATCTGAT
 1081 TGCCACTTCA CAGGTCTGGG GGCCATCCAG CCAGCTTCCA GGCAAGAATT CCTTGGGGCC
1141 TGTTTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
user report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
user report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Advances 1: 1440-1451 (2017). PMID 29296785.
user report Lee, J., Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation. Cell Stem. Cell, 5, 76-86 (2009). PMID 19570516.

2018.10.22

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