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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phGLI1

Promoter collection, Human GLI1 promoter

Catalog number RDB07902
Resource name pGL4-phGLI1
Alternative name U3030-3
Clone info. PCR amplified human GLI1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 57458742 to 57460211(NC_000012.12); relatively -1043 to +427, where +1 corresponds to 1 nt of NM_005269.3; corresponding to 1 to 51nt of NM_005269.2, 10 bp of intron and 1.4 kb up-stream; relatively -1396 to +74, where +1 corresponds to 1 nt of XM_011538190.2.
Comment PCR cloning, forward primer: tgcccattagtctggtctga; reverse primer: cccttctcacctctgtctgg. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human GLI family zinc finger 1 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human GLI family zinc finger 1

          Reference sequence
            

          Distribution information

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          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.  2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07902 pGL4-phGLI1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07902_A7Jvp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Region: insert 5'
          Sequence file: RDB07902_A7Jvd.seq check
          >07902_07902_A7Jv_pGL4-4174F_A03_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNG GNCNNNTGGC CGGTNCCTGN NCTCGCTAGC CTCGAGGANT
             61 GCCCATTAGT CTGGTCTGAC TGAGCTGGGT CTCTGACCCT CTGGGGATAA CTAGCCTGGG
            121 TCAAAGTCCC AGATCTCCCC CTACCTTCAC CTTTTCTTTT CCCCCTTGAC CCTCAGACTG
            181 AACAGTTAAC CCACTGACCT TCCACACCCA AGGGGGTGGT TCTTGGAAGC AGAGCTAGGA
            241 TGTGGGAGGT CTGCCTGTGG GGTTGAAAAA AAGGGGAGAG GGTGCCCTTT TTCCTTGTCA
            301 TGCTTCCTCC TCTTTCTCAT AAAAATCAGA GACTGAAATG CTGCCCCTCC CTTATATCCG
            361 GTCACGATGG CAATGCAAAT CTAAAGAGGC AGGGCACTTC CCTGTCAGGC AGTACCGCTG
            421 GGCATAGCAA CCTCTGCCTC TCCGTTTCTC AGAGCTCACA TATCCACCTC CTGGGCTTTT
            481 AAGTGGGCTT TAGTGAGGGG CTCCTCCTTC AACTGGGCTC CTCCTTCAGT TCCCCAGCTC
            541 TTCTGCTTCG ACTCCGAGCG GGTGTCATGT GTGAGAACGG CCAGCAGAGG GAGCAGAAAG
            601 CCTGGAAGAG CAGCTAGAGC CTGCAGTGAC GTGGTGCGGA GGGGCGGCAC CCTCCAGAAC
            661 TTCGAGACGT AGAGCCGGGG TTCTAGGGAA AGGGGCTTCA GTCCCAGGGC TCCTTGGTGA
            721 CCTCGTGAAC CACACCCTGC ACCCAGAGCC TCAGCCGCTG CTCCTTGCTT TTATGCTCCA
            781 TAGACTCCTC ACCTTCTTCC AGANCCCCCN ACCCAACTTN ATTTGCCCCA AACCGCAACT
            841 CTGTCCCGGC CGCNGCAAGT TCCATCCAAA NGGNGANGCC NGCAGATAAA CCNCAGGATG
            901 GNANAANGCT CANNTAGCAC CNNNNNNNNG CGANNANCNN ACCTCCNCNN TTANNNNNNN
          961 NNN
          //
          Primer: pGL4ins_R
          Region: Luc 5',insert 3'
          Sequence file: RDB07902_A7Jve.seq check
          >07902_07902_A7JvpGL4ins_R_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN GGGNANNANG GCGCTGGGCC CTTCTTAATG TTTTTGGCAT
             61 CTTCCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC TTGGCCGCCG AGGCCAGATC
            121 TTGATCCCTT CTCACCTCTG TCTGGGCGCT GGGCTCGGGA TGCGCGGTCC AGGGCTGGAG
          181 TCTGGGCTGG GGGGTGTGCG GTGGGGAGGN N
          //
          Primer: pAxCALNLF1
          Region: Luc 3'
          Sequence file: RDB07902_A7Jvf.seq check
          >07902_07902_A7Jv_pAxCALNL_F1_C03_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGNTCNAN CGGCCGGCCG CCCCGNCTCT AGAATTATTA
             61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC
            121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT
            181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT
            241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC
            301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG
            361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG
            421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG
            481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA
            541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC
            601 CTCGAANAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT
            661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGANGCCTG GTAGGTGGAA
            721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC
            781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GANNNTGCTC TTANCGAANA NNCTAAATAN
            841 TGTGGGCACC AGCNNGGGCA NANTGAATCT TATAGTCTTG CANGCTGCGC NAGAANANCT
            901 CCNCCTCGAA NCGGNNCATG ANCNCNNNCC NNNANCNNNN NNTCANGTAG CCNNNNNNNG
          961 NNNNNNTGCN NNANNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Patel, R., Novel GLI3 pathogenic variants in complex pre- and postaxial polysyndactyly and Greig cephalopolysyndactyly syndrome Am. J. Med. Genet. A 185 (1): 97-104 (2021). PMID 33058447.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl