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pGL4-phGLI1 (#RDB07902)

Promoter collection, Human GLI1 promoter

Alternative name U3030-3
Clone info. PCR amplified human GLI1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 57458742 to 57460211(NC_000012.12); relatively -1043 to +427, where +1 corresponds to 1 nt of NM_005269.3; corresponding to 1 to 51nt of NM_005269.2, 10 bp of intron and 1.4 kb up-stream; relatively -1396 to +74, where +1 corresponds to 1 nt of XM_011538190.2.
Comment PCR cloning, forward primer: tgcccattagtctggtctga; reverse primer: cccttctcacctctgtctgg. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human GLI family zinc finger 1 genomic DNA
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RDB07902 pGL4-phGLI1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phGLI1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07902).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07902_A7Jvp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F (Pr0081)
Region: insert 5'
Sequence file: RDB07902_A7Jvd.seq check
>07902_07902_A7Jv_pGL4-4174F_A03_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG GNCNNNTGGC CGGTNCCTGN NCTCGCTAGC CTCGAGGANT
   61 GCCCATTAGT CTGGTCTGAC TGAGCTGGGT CTCTGACCCT CTGGGGATAA CTAGCCTGGG
  121 TCAAAGTCCC AGATCTCCCC CTACCTTCAC CTTTTCTTTT CCCCCTTGAC CCTCAGACTG
  181 AACAGTTAAC CCACTGACCT TCCACACCCA AGGGGGTGGT TCTTGGAAGC AGAGCTAGGA
  241 TGTGGGAGGT CTGCCTGTGG GGTTGAAAAA AAGGGGAGAG GGTGCCCTTT TTCCTTGTCA
  301 TGCTTCCTCC TCTTTCTCAT AAAAATCAGA GACTGAAATG CTGCCCCTCC CTTATATCCG
  361 GTCACGATGG CAATGCAAAT CTAAAGAGGC AGGGCACTTC CCTGTCAGGC AGTACCGCTG
  421 GGCATAGCAA CCTCTGCCTC TCCGTTTCTC AGAGCTCACA TATCCACCTC CTGGGCTTTT
  481 AAGTGGGCTT TAGTGAGGGG CTCCTCCTTC AACTGGGCTC CTCCTTCAGT TCCCCAGCTC
  541 TTCTGCTTCG ACTCCGAGCG GGTGTCATGT GTGAGAACGG CCAGCAGAGG GAGCAGAAAG
  601 CCTGGAAGAG CAGCTAGAGC CTGCAGTGAC GTGGTGCGGA GGGGCGGCAC CCTCCAGAAC
  661 TTCGAGACGT AGAGCCGGGG TTCTAGGGAA AGGGGCTTCA GTCCCAGGGC TCCTTGGTGA
  721 CCTCGTGAAC CACACCCTGC ACCCAGAGCC TCAGCCGCTG CTCCTTGCTT TTATGCTCCA
  781 TAGACTCCTC ACCTTCTTCC AGANCCCCCN ACCCAACTTN ATTTGCCCCA AACCGCAACT
  841 CTGTCCCGGC CGCNGCAAGT TCCATCCAAA NGGNGANGCC NGCAGATAAA CCNCAGGATG
  901 GNANAANGCT CANNTAGCAC CNNNNNNNNG CGANNANCNN ACCTCCNCNN TTANNNNNNN
961 NNN
//
Primer: pGL4ins_R (Pr0666)
Region: Luc 5',insert 3'
Sequence file: RDB07902_A7Jve.seq check
>07902_07902_A7JvpGL4ins_R_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGGNANNANG GCGCTGGGCC CTTCTTAATG TTTTTGGCAT
   61 CTTCCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC TTGGCCGCCG AGGCCAGATC
  121 TTGATCCCTT CTCACCTCTG TCTGGGCGCT GGGCTCGGGA TGCGCGGTCC AGGGCTGGAG
181 TCTGGGCTGG GGGGTGTGCG GTGGGGAGGN N
//
Primer: pAxCALNLF1 (Pr0166)
Region: Luc 3'
Sequence file: RDB07902_A7Jvf.seq check
>07902_07902_A7Jv_pAxCALNL_F1_C03_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGNTCNAN CGGCCGGCCG CCCCGNCTCT AGAATTATTA
   61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC
  121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT
  181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT
  241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC
  301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG
  361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG
  421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG
  481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA
  541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC
  601 CTCGAANAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT
  661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGANGCCTG GTAGGTGGAA
  721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC
  781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GANNNTGCTC TTANCGAANA NNCTAAATAN
  841 TGTGGGCACC AGCNNGGGCA NANTGAATCT TATAGTCTTG CANGCTGCGC NAGAANANCT
  901 CCNCCTCGAA NCGGNNCATG ANCNCNNNCC NNNANCNNNN NNTCANGTAG CCNNNNNNNG
961 NNNNNNTGCN NNANNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Hamada, T., MALAT1 functions as a transcriptional promoter of MALAT1::GLI1 fusion for truncated GLI1 protein expression in cancer. BMC Cancer 23 (1): 424 (2023). PMID 37165307. [link to RRC of NBRP]
user_report Patel, R., Novel GLI3 pathogenic variants in complex pre- and postaxial polysyndactyly and Greig cephalopolysyndactyly syndrome Am. J. Med. Genet. A 185 (1): 97-104 (2021). PMID 33058447. [link to RRC of NBRP]