Resource data sheet
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CS-CA-RfA-IRES2-Venus

Shuttle vector to generate recombinant Lentiviral vector with Venus as a marker.

Catalog number RDB07894
Resource name CS-CA-RfA-IRES2-Venus
Clone info. Shuttle vector to generate recombinant Lentiviral vector. Gateway(R) destination vector with Venus as a marker.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Amp^r
Gene/insert name Aequorea victoria cDNA
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
(Japanese text [open/close])
Remarks ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08996_A3Egp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB08996_A3Ega.seq
>07894_08996_A3Eg_pAxCA_F1_C01_07.ab1
    1 ACTTGACCTA GGGGGACCAA CTGCTCATGT TATGCCTTCT TCTTTTTCCT ACAGCTCCTG
   61 GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGAT TTGATACGTA
  121 CGGTTGGGCT GCAGGAATTC TGCAGATATC CAGCACAGTG GCGGCCGCTC GAGAATTCTG
  181 CAGCGGCCAT CACAAGTTTG TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA
  241 TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC
  301 ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
  361 CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC
  421 TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA
  481 AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT
  541 GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT
  601 TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA
  661 CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC
  721 TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT
  781 ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT
  841 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTTGATTTAA
  901 ACGTGGCCAA TATGGACAAC TTCTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC
  961 AAGGCGACAA GGTGCTGATG CCCGCTGGCG ATTTCAAGGT TCATCATGCC GTTTGTGATG
 1021 GGCTTCCCAT GTCGGGCAGA ATGCTTTAAT GGAATTAACA ACCAGTACCT GCGATGAAGT
 1081 GGCAGGCGGG GACGTAAACG GCGATGGAAT CCCGGGCTTA ACTTAAAAAG GCCCAGAATT
1141 TAAACAG
//
Primer: IRES-R RDB08996_A3Egb.seq
>07894_08996_A3Eg_IRES-R_D01_10.ab1
    1 GCCAGGGCGG GCTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGCGG ATCCGCGGCC
   61 ATCACCACTT TGTACAAGAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA
  121 TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG
  181 TCACTATGGT CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT ATATTCCCCA
  241 GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT CAGCCACTTC
  301 TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC GCCAGCTTTC
  361 ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA GCAGACGTGC
  421 ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC TCTGTACATC
  481 CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT GCATTTCACC
  541 AGCCCCTGTT CTCGTCAGCA AAAGAGCCGT TCATTTCAAT AAACCGGGCG ACCTCAGCCA
  601 TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT CGGCACGCAG ACGACGGGCT TCATTCTGCA
  661 TGGTTGTGCT TACCAGACCG GAGATATTGA CATCATATAT GCCTTGAGCA ACTGATAGCT
  721 GTCGCTGTCA ACTGTCACTG TAATACGCTG CTTCATAGCA TACCTCTTTT TGACATACTT
  781 CGGGTATACA TATCAGTATA TATTCTTATA CCGCAAAAAT CAGCGCGCAA ATACGCATAC
  841 TGTTATCTGG CTTTTAGTAA GCCGGATCCA CGCGTTTACG CCCCGCCCTG CCACTCATCG
  901 CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGG AAGCCATCAC AAACGGCATG
  961 ATGAAACCTG AAATCGCCCA GCGGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC
 1021 CCATGGTGAA AAACGGGGGG CGAAGAAGTT TGTCCATATT GCCACGGTTT TAATCAAACT
1081 GATGAACTCC ACCCAAGGGA TTTGGACTGA GACCGAAAAA AACCATTAAT
//
Primer: IRES-F RDB08996_A3Egc.seq
>07894_08996_A3Eg_IRES-F_C10_07.ab1
    1 GGGAGCGTCA TCTTTATGTG TTTAGTCGAG GTTAAAAAAC GTCTAGGCCC CCCGAACCAC
   61 GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG GCCACAACCA TGGTGAGCAA
  121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
  181 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
  241 CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
  301 CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT
  361 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
  421 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
  481 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
  541 CAACTACAAC AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC
  601 CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA
  661 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA
  721 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
  781 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAG AATTCAAGGC
  841 CTCTCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT
  901 GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC
  961 TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTTCATTTT TCTCCCTCCC TTGTATAAAT
 1021 CCTGGATTGC TGTCTCTTTT ATGAGGAGTT GTGGGCCCCG TTTGTCAGGC AACGTGGCGT
1081 GGCTGTGCAC TGAGATTTGC TGGACGCAAC CCCCCCCACT TGGAATTTG
//
Primer: CMV-Forward RDB08996_A3Egd.seq
>07894_08996_A3Eg_CMV-Forward_D10_10.ab1
    1 CGTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GCGGCCGCTG
  781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AAGGCAAAGA GAAGAGTGGT
  901 GCAGAGAGAA AAAAAGAGCA GTGGGGAATA GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA
  961 AGCAGGAAGC ACTATGGGGC GCAGCGTCAA TGACGCTGAC GGGTACAGGC CCAGACATTT
 1021 ATTGTCTGGG TATAGTGCAG CAGCAGGACC ACTTTGCCTG AGGGCTTATT GGAAGCGCAT
1081 CAAGCAATCT GATGCTAGCT CACAGGTCTG AGCAAATCAA GCAGGTCCAT TTTCA
//
Primer: BGH_rev RDB08996_A3Ege.seq
>07894_08996_A3Eg_BGHrev_E10_13.ab1
    1 GATCGGGGTC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA
   61 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA
  121 GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC
  181 CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA TGTGATGAAA TGCTAGGCGG
  241 CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT CCATCCCATG CAGGCTCACA
  301 GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT CTACCTTCTC TTGCTCAACT
  361 GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC TGATCCCTGG CCCTGGTGTG
  421 TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA TCCACAGATC AAGGATATCT
  481 TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA
  541 AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA CCTGAGGTGT GACTGGAAAA
  601 CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT CAGCATTGGT AGCTGCTGTA
  661 TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC GGTATCGATG CGGGGAGGCG
  721 GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAAGCGAA GACGCGGAAG AGGCCGCAGA
  781 GCCGGCAGCA GGCCGCGGGA AAGGAAGGTC CGCTGGATTG AGGGCCGAAG GGACGTAGCA
  841 GAATGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG AGCAGCCATG GAAAGGACGT
  901 CAGCTTCCCC GACACACCCA CGGAATGTCA GTGCTCAACA GCCGAGCTCC CTGTCAGCAG
  961 CCGGGCAGCA GCGCGATGAG TCGTCGTGCA ATAGGGAGGG GGAAGCGAAG GTCCTGAAAG
 1021 AGCTGACAGG TGTGCATGCC TATCATGGGG ATGCTCAGCA AACCATGCAC TAGTCAGTGC
1081 TGACACTGTC AAATCTCTAA GAAGACGC
//
Primer: SV40pro_F_V2 RDB08996_A3Egf.seq
>07894_08996_A3Eg_SV40pro_F_V2_E01_13.ab1
    1 TATGGCTTTT TTGGAGGCGT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGT ATATCCATTT
   61 TCGGATCTGA TCAGGGGGCT GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT
  121 ACGACAAGGT GAGGAACTAA ACCATGGCCA AGTTGACCAG TGCCGTTCCG GTGCTCACCG
  181 CGCGCGACGT CGCCGGAGCG GTCGAGTTCT GGACCGACCG GCTCGGGTTC TCCCGGGACT
  241 TCGTGGAGGA CGACTTCGCC GGTGTGGTCC GGGACGACGT GACCCTGTTC ATCAGCGCGG
  301 TCCAGGACCA GGTGGTGCCG GACAACACCC TGGCCTGGGT GTGGGTGCGC GGCCTGGACG
  361 AGCTGTACGC CGAGTGGTCG GAGGTCGTGT CCACGAACTT CCGGGACGCC TCCGGGCCGG
  421 CCATGACCGA GATCGGCGAG CAGCCGTGGG GGCGGGAGTT CGCCCTGCGC GACCCGGCCG
  481 GCAACTGCGT GCACTTCGTG GCCGAGGAGC AGGACTGACA CGTGCTACGA GATTTCGATT
  541 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA
  601 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG
  661 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
  721 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA
  781 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA
  841 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAA GTGTAAAGCC
  901 TGGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
  961 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGGGAAG
 1021 AGGCGGTTTT GCGTATTGGG GCGGCTTCTT TCCCGCTTCC TCGGCTCAAC TGGAACTCGC
1081 CTGCGCTCGG ATCGATTCGG CTGCCGGCGA AGCCGGTTAA TTCAGGCCTT CCAAC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References


2017.10.23

GNP_filter3_RDBDEP_171017_html.pl