Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-CA-RfA-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by CAG promoter by Gateway(R) cloning with Venus fluorescent protein marker.

Sequence (full)	RDB07894zzk02.seq provided by the depositor
Remarks, protocol and/or map (pdf)	RDB07894.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 付加的提供条件： 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 宮脇敦史博士による提供承諾書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08996_A5Kip1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: attR1,CmR 5' Sequence file: RDB08996_A5Kid.seq
>07894_08996_A5Ki_pAxCA_F1_D05_11.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCCTTCTT CTTTTTNCNN ANNNNNNNNG    61 GGNNACGTGC TGGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTGA TTTGATACGT   121 ACGGTTGGGC TGCAGGAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCT CGAGAATTCT   181 GCAGCGGCCA TCACAAGTTT GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT   241 ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA   301 CATATCCAGT CACTATGGCG GCCGCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG   361 GCTCGTATAA TGTGTGGATT TTGAGTTAGG ATCCGTCGAG ATTTTCAGGA GCTAAGGAAG   421 CTAAAATGGA GAAAAAAATC ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA   481 AAGAACATTT TGAGGCATTT CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCAGC   541 TGGATATTAC GGCCTTTTTA AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT   601 TTATTCACAT TCTTGCCCGC CTGATGAATG CTCATCCGGA ATTCCGTATG GCAATGAAAG   661 ACGGTGAGCT GGTGATATGG GATAGTGTTC ACCCTTGTTA CNCCGTTTTC CNTGAGCAAA   721 CTGAAACGTT TTCATCGCTC TGGAGTGAAT ACCACGACGA TTTCCGGCAG TTTCTACNCA   781 TATATTCNNA AGATGTGGCG TGTTACGGTG AAAANNNGGC CTANTTCCCT AAANGGTTTA   841 TTGAGAATAT GNTTTTCGTC TCANCCNANT CCCTGGGGTG AGTTNCNNCA GTTTTGANTT   901 AANNGTNGCC NNNATGGACA ACTTCTTCGC CCCCNNTTTT CACNATNNNN ANNATTATAC   961 GCNANNGCGA NNANGNGCTG ATGCCGCNGG CGANTNNNNT CATCATGCCN NNNGTGANGN 1021 NNTNCNNTNN NNNNANNNNN NNTNANNGNA NNNNNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,Venus 3' Sequence file: RDB08996_A5Kie.seq
>07894_08996_B3AcS_PRE_rev_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNCGNNNNG GAGCNNCNTA GTTAAGAATA CCNGTCAATC TTTCNCAAAT    61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGAGAGGCCT TGAATTCTTA   121 CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC   181 CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG GACTGGTAGC TCAGGTAGTG   241 GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC   301 GAGCTGCACG CCGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTGGCCT TGATGCCGTT   361 CTTCTGCTTG TCGGCGGTGA TATAGACGTT GTGGCTGTTG TAGTTGTACT CCAGCTTGTG   421 CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC GGTTCACCAG   481 GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA   541 GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT   601 CATGTGGTCG GGGTAGCGGG CGAAGCACTG CAGGCCGTAG CCCAGGGTGG TCACGAGGGT   661 GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATCAGC TTCAGGGTCA GCTTGCCGTA   721 NGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAACTTG TGGCCGTTTA CGTCGCCGTC   781 CAGCTCGACC AGGATGGGCA CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCNTGGT   841 TGTGGNNATA TTATCATCGT GTTTTTCAAA GGAAAANNNA CGTCCCCGTG GTTCNGGGGG   901 NCTANACGTT TTTTTNNNCT CGANTAANNN NNTGTNAANC NNGTGTNNCN GANGNCCCNN   961 ATCNNATCCC NTACANNGGG GGTANCTTCN GGNCANCNNT CNNNCCCTTG NNNAANANNC 1021 TTGNNGNNNN CCNTTNNANN CNTTNCNNNN NNNTCNANTN NNNNNNNNGN NNNNNNNNN //
Primer: IRES-R (Pr0037) Region: attR2,ccdB Sequence file: RDB08996_A5Kif.seq
>07894_08996_A5Ki_IRES-R_E05_14.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTNN GGGGGGGGGG NNGGNNAGGG GCGGNTCCGC    61 GGCCNTCACC ACTTTGTACN AGAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA   121 TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT   181 CCAGTCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC ATTTATATTC   241 CCCAGAACAT CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG AGATCAGCCA   301 CTTCTTCCCC GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC ATGCGCCAGC   361 TTTCATCCCC GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT GACAGCAGAC   421 GTGCACTGGC CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA TCACTCTGTA   481 CATCCACAAA CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA AACTGCATTT   541 CACCAGCCCC TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG GGCGACCTCA   601 GCCATCCCTT CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG GGCTTCATTC   661 TGCATGGTTG TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG AGCAACTGAT   721 AGCTGTCGCT GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC TTTTTGACAT   781 ACTTCGGGTA TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC GCAAATACGC   841 ATACTGTTAT CTGGCTTTTA GNNAGCCGGA NCCACGCGTT TACGCCCCGC CCTGCCACTC   901 NTCGCAGNAC TGTTGTANNT CANTAANCAT TCTGNCGACN TGGNANNCNN TCANAANCGG   961 NATGATGAAC CTGANNCGNN NGCGNCNTCN NCNNCNNNNN NNNCNNNGCG NNTNANANTT 1021 GNCCNNNGNN GAAAACNGGG GNNNNNNAAN NNNGNNNNNN NNNN //
Primer: IRES-F (Pr0036) Region: IRES2,Venus 5' Sequence file: RDB08996_A5Kig.seq
>07894_08996_A5Ki_IRES-F_F05_17.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCGAGG TNNNNNNNNN TCTAGGCCCC CCGAACCNCG    61 GGGANGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG   121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC   181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC   241 CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC   301 CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC   361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC   421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC   481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC   541 AACTACAACA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC   601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG   661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC   721 CAGTCCGCCC TGANCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC   781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGANCTGT ACAAGTAAGA ATTCAAGGCC   841 TCTCGANGGA TCAAGCTTAT CGATAATCNA NNCTCTGGAT TACNAAATTT GTGAAANATT   901 GACTGGTATT CTTAACTATG TTGCTCCTTT NACGCTATGT GGATACNCTG CTTTNNNGNC   961 TTTGNATCAT GCTATTGCTN NCCGNATGGN TTTCATTTTC TNNNCCNTNG NNNAANNNCN 1021 NNNNNNGCTG NNNNNTTNAT GNNNANNNNN NGNNNNNNNN NN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 psi Sequence file: RDB08996_A5Kih.seq
>07894_08996_B3AcS_CMV-Forward_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNTGNNN NNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT   121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG   181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT   241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC   301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG   361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG   421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT   481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT   541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT   601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC   661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT   721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCTGA   781 TCTTCAGACC TGGAGGAGGA GATATGANGG ACAATTGGAG AAGTGAATTA TATAAATATA   841 AAGTAGTAAA AATTGAACCN TTAGGAGTAG CACCCACCNA GGCAAAGAGA AGANTGGTGC   901 AGANAGAAAA AAGANCANNG GGAATANGAN CNTTGTNNNN NGGGTNCNNG GGAGCNNCNN 961 NAAGCNCTAT GGGCGCANNN NNNNTGNNNC NNNNNGNNNN NNNNNN //
Primer: cPPT/CTS-E_R (Pr0725) Region: cPPT_CTS,RRE Sequence file: RDB08996_A5Kii.seq
>07894_08996_B3AcS_cPPT_CTS-E_R_C02_08_ABI24.ab1     1 NNNNNNNNNN NNGNTTTTNN NNNCTTNAGT TTNTATGTCT GTTGCTATTA TGTCTACTAT    61 TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC   121 TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT   181 CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG   241 ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC   301 TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG   361 TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCAATAAT TCTTGTTCAT   421 TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG TATTAAGCTT GTGTAATTGT   481 TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA AATCTGTTCC AGAGATTTAT   541 TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT GAGTTTTCCA GAGCAACCCC   601 AAATCCCCAG GAGCTGTTGA TCCTTTAGGT ATCTTTCCAC AGCCAGGATT CTTGCCTGGA   661 GCTGCTTGAT GCCCCAGACT GTGAGTTGCA ACAGATGCTG TTGCGCCTCA ATAGCCCTCA   721 GCAAATTGTT CTGCTGCTGC ACTATACCAG ACAANAATTG TCTGGCCTGT ACCGTCAGCG   781 TCATTGACGC TGCGCCNATA NTGCTTCCTG CTGCTCCNNN NAANNNNNNG AACAAANCTC   841 CTATTCCCAC TGNTCNNNNN NNNCTCTNNA CNNNTCNTCT CTTTNNCCTT GNNGGGNGCT 901 NNNNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA,3' LTR Sequence file: RDB08996_A5Kij.seq
>07894_08996_B3AcS_bGH_rev3_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNGNNNGGN NGNNGGGCNN ACNACAGATG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA   121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG   181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC   241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC   301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC   361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC   421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA   481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA   541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC   601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG   661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA   721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG   781 ACGGTATCGA TGCGGGGAGG CGGCCCAAAG GGAGATCCGA CTCGTCTGAN GGCGAANGCG   841 AAGACGCGGA AGANGCCGCA GANCCGGCAN CAGGNCGCGG GAAGGNANGG TCCNCTGGAT   901 TGAGGGCCGA ANGGANNTAN NANAANGACN TNCCGCGCAN AATCCAGGNG NNANNNNNGG   961 CGANCAGCCA NNGNNANGNN NGNNNGCTTC CCCGACAACN NCNCNNNNNN GNCNGNGCCC 1021 NNNNNNNNAG CCCNNNNNNN GCANNGGNNA NNNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori,Zeo Sequence file: RDB08996_A5Kik.seq
>07894_08996_B3AcS_SV40pro_ori_F_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNN CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC   121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC   181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC   241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG   301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG   361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG   421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG   481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC   541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCNCC   601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC   661 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT   721 TATAATGGTT ACAAATAAAG CAATAGCATN NCANATTTCA CAAATAAAGC ATTTTTTTCA   781 CTGCATTCTA GTTGTGGTTT GTCCNAACTC ATCAATGTAT CNTATCATGT CTGTATACCG   841 TCNNNCTCTA NCTAGAGCTN NNNGNNNNCN TGGTCATAGC TGTTTNCTGT GTGNAANTNN   901 TATCCGCTCN CAATTNNNNN NNACNTNCNA GCCGGANNCN TAAAGNGNAA ANNCNNGGGG 961 NGNCNNANNN NGTGNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl