Resource data sheet
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pGL3-AID R3

Reporter construct of mouse Aicda locus

Catalog number RDB07881
Resource name pGL3-AID R3
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, not applicable.
Nat. Immunol. (2010) 2, 148-154, no figure.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07881 pGL3-AID R3 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07881_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB07881_A4Gia.seq
>07881_07881_A4Gi_pGL4-4174F_E06_15.ab1
    1 ATGGGGGTCA GACATTTCTC TATCGATAGG TACCGAGCTC TTACGCGTGC TAGCCCGGGT
   61 CGAGTTTAGA GGAACAGCCT CGAAGGAAGT TGGACATTAA GCATGAGCAG AACTGGCCCC
  121 CCCCCCCCAA TCATTTAATC CGTGTGGCTT TGCCCACCAC AGCCCCGCCC ATCTTTACTG
  181 GACCCAACCC AGGAGGCAGA TGTTGGATAC CTGGTGGTAG TGATGCTGTC GTGGGGGAGG
  241 AGCCCACAAG AGCAAGCTCA GATTTGAATG CCAGGGGCCA GTGCTCTGTC ACACAACAGC
  301 ACTGAAGCAG CCTTGCTTGA AGCAAGCTTC CTTTGGCCTA AGACTTTGAG GGAGTCAAGA
  361 AAGTCACGCT GGAGACCGAT CCATGGAAGA CGCCAAAAAC ATAAAGAAAG GCCCGGCGCC
  421 ATTCTATCCG CTGGAAGATG GAACCGCTGG AGAGCAACTG CATAAGGCTA TGAAGAGATA
  481 CGCCCTGGTT CCTGGAACAA TTGCTTTTAC AGATGCACAT ATCGAGGTGG ACATCACTTA
  541 CGCTGAGTAC TTCGAAATGT CCGTTCGGTT GGCAGAAGCT ATGAAACGAT ATGGGCTGAA
  601 TACAAATCAC AGAATCGTCG TATGCAGTGA AAACTCTCTT CAATTCTTTA TGCCGGTGTT
  661 GGGCGCGTTA TTTATCGGAG TTGCAGTTGC GCCCGCGAAC GACATTTATA ATGAACGTGA
  721 ATTGCTCAAC AGTATGGGCA TTTCGCAGCC TACCGTGGTG TTCGTTTCCA AAAAGGGGTT
  781 GCAAAAAATT TTGAACGTGC AAAAAAAGCT CCCAATCATC CAAAAAATTA TTATCATGGA
  841 TTCTAAAACG GATTACCAGG GATTTCAGTC GATGTACACG TTCGTCACAT CTCATCTACC
  901 TCCCGGTTTT AATGAATACG ATTTTGTGCC AGAGTCCTTC GATAGGGACA AGACAATTGC
  961 ACTGATCATG AACTCCTCTG GGATCTACTG GTCTGCCTAA AGGTGTCGCT CTGCTCATAG
 1021 ACTGCCTGCG TGAGATCTCG CATGCAGAGA TCCTATTTTT TGGCATCCAA TCAATTTCGG
1081 GATACTGCGA TTTAATGTGT TCCATCATCA CGATTTGAAA TGTTTACCTA C
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

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