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pGL3-AID R4 del.-17

Reporter construct of mouse Aicda locus, position -9226 to -8357 plus -8243 to -7585.

Catalog number RDB07871
Resource name pGL3-AID R4 del.-17
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -8357 plus -8243 to -7585.
Nat. Immunol. (2010) 2, 148-154, Fig. 4g.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07871 pGL3-AID R4 del.-17 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12850_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB12850_A4Gia.seq
>07871_12850_A4Gi_3_pGL4-4174F_F04_16.ab1
    1 GGTGGGTCAG ACATTTCTCT ATCGATAGGT ACCCACCACT CTCGGCTGTT CTGGAACTCT
   61 CTGTAACCTC GGGCTGTATG AGTGTATGTG AGTGTGTGTA TATGGGTGTG GGGGGGGTGA
  121 GAGTATATGA GTGTATGTGA GTGTGTATGT ATGGGTGTGA GGTGTGTGTG TGTGTATGAG
181 TGTGTGTATG AGTGTGTGTA TGAGTGTGTG TGAGTGTGTG TATATGGGGG GGTGAGG
//
Primer: Luc_R RDB12850_A4Gib.seq
>07871_12850_A4Gi_3_Luc_R_G04_19.ab1
    1 CATGATTCAG GACCAGGGCG TATCTCTTCA TAGCCTTATG CAGTTGCTCT CCAGCGGTTC
   61 CATCTTCCAG CGGATAGAAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATGG
  121 ATCGGTCTCC AGCGTGACTT TCTTGACTCC CTCAAAGTCT TAGGCCAAAG GAAGCTTTTC
  181 TTCAAGCAAG GCTGCTTCAG TGCTGTTGTG TGACAGAGCA CTGGCCCCTG GCATTCAAAT
  241 CTGAGCTTGC TCTTGTGGGC TCCTCCCCCA CGACAGCATC ACTACCACCA GGTATCCAAC
  301 ATCTGCCTCC TGGGTTGGGT CCAGTAAAGA TGGGCGGGGC TGTGGTGGGC AAAGCCACAC
  361 GGATTAAATG ATTGGGGGGG GGGGCCAGTT CTGCTCATGC TTAATGTCCA ACTTCCTTCG
  421 AGGCTGTTCC TCTAAACTCG AGCTGTAGCA CACGCACACG CACGCCCACA CCCTCTCTTA
  481 CAGATGCCGT ACACATGCCA TAGGAACAAA TACAAAACCC TTGATTTGTA AGAAAGCCAA
  541 ATTCTCTCAT TTGCCTTCTT CTAAAGGAAG GAAAGAGATC TCACTTAGTT ACAAAAACCA
  601 TTTTCCAGTC CCGGGAAATT TGGAACTGGT ACAGCGGCAA AGCAGTTTAC AGGGTTTTGA
  661 TCAACTTTTA TTTTCTTCAT TAACTTCTCT TTCCCCTCAT TAACTTCTAT TGAAAAACCA
  721 CAAGCCAACT TGGTCACATT GTTTTTAACC AAAAATACTG TGACTTGGAA ATCGAGTCGC
  781 CTGCGGCTGA GCTGAGTTTT GGACATCTGG AGTTCACCAC CTCGCTTTCC CTATGAGACA
  841 CACAGGTGAG GTTTCGTTAT GGCCAGCATG AGGGGCTGGC GGTCTCTGGA AAGCCTCGCG
  901 GTGGGGGGCC TGCATTCTTG GTGTCTCAAG AGCCAGCACA CTCAGACATG ACTCCTCGAG
  961 CCCGGACTAG CACGGCGTAA GAGCTCGGTA CCAACTTCTG CATTCCCGGA TTTTTTGTTT
 1021 TTTCTCACTT TGTGTCCCTG CAGGTACTGG CATCCCTTCT GGGTATGGAA AGGCATGGAC
1081 AGTCCTCGAT GACCGGAATT ACTCAGCTTC TGCCTGATGA GCCTGC
//
Primer: R4del-M_R2 RDB12850_A4Gic.seq
>RDB07871_12850_A4Gi_R4del-M_R2_3_B01_04.ab1
    1 CAGGCGGGCG GTCTCTGGAG CCTCGGGGGG GGGGGGGGGC TGCATTCCTG TGTCTCAAAG
   61 CCAGCACACT CAGACATGAC TCCTCGAGCC CGGGCTAGCA CGCGTAAAAG CTCGGTACCA
  121 ACTTCTGCAT TCCCCGATTT TTGTTTTTCT CACTTTGTGG CCCTGGAGGT TACTGCATCC
  181 CTCTGGGGTA TGAAAAGCCA TGGGACAGCC CTCAAACACA GACCAAAATT ACTCAGCTTC
  241 TGCTGTTGGG CTGCCCAGGA CATTTCAAAG ACCAAAGAAC TGTAAACAGG TATTTATCAA
  301 AAGCTTAGGT CTACGGGGAG GTGGAGGCAG GAGGATTGAG AGTTCAAGGC CATCTTCTGC
  361 CTCGTGTTGA ATTCAAGGCC AGCAGGCAGG TTTGAAAGAA AAAAAAAATT TTCAAATGGA
  421 ATACTATGAA ATTAAAAGGC TTCAAACTGA GTGTGGTGAC TAATCCTAGC AGCCTGGAGG
  481 TTGAGGCAGG AGGATTGTCA AGAGTTGGAA ACCACCTTGA GCTCCATCCC AGGACCCACA
  541 TGGTAGGGGG AGTGAACCAA TTCATGCAAG TTGTCCACTG ACCTCTACGC TCATACACAC
  601 ACACACTCAT ACACACACCC ATAATACACA CTCACACACA CTCATACACA CACCCATACA
  661 CACACTCATA CACACACACA CCCACACACA CACCCATACA TACACACTCA CATATACTCA
  721 TACACCCTCA TCCCCACACC CATATACACA CACTCACACA CACTCATACA CACACTCATA
  781 CACACACACC TCACACCCAT ACATACACAC TCACATACAC TCATATACCC TCACCCCCCC
  841 CATATACACA CACTCACACA CACTCATACA CACACTCATA CACACACTCA TACACACACA
  901 CACACCTCAC ACCCATACAT ACACACTCAC ATACACTCAT ATACTCTCAC CCCCCCCACA
  961 CCCATATACA CACACTCACA TACACTCATA CAGCCCGAGG GTTACAGAGA GTTTCCAGAT
 1021 CAGCCGAGAG TTGGTGGGTT ACCTATCGAT AAGAGAAATG TTCTGGCACC TTGCACTTGG
1081 CACTGAGACA GCTATTTTGC TAGTGTTGTT CGTTTTGTTT GATGGAAGCG ATGTTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

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