Resource data sheet
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pGL3-AID R4 del.-15

Reporter construct of mouse Aicda locus, position -9226 to -7910.

Catalog number RDB07869
Resource name pGL3-AID R4 del.-15
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -7910.
Nat. Immunol. (2010) 2, 148-154, Fig. 3c.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07869 pGL3-AID R4 del.-15 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07869_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB07869_A4Gia.seq
>07869_07869_A4Gi_pGL4-4174F_E04_13.ab1
    1 GTTTAGGTTC AAGAACATTT CTCTATCGAT AGGTACCCAC CACTCTCGGC TGTTCTGGAA
   61 CTCTCTGTAA CCTCGGGCTG TATGAGTGTA TGTGAGTGTG TGTATATGGG TGTGGGGGGG
121 GTGAGAGT
//
Primer: Luc_R RDB07869_A4Gib.seq
>07869_07869_A4Gi_Luc_R_F04_16.ab1
    1 CATTGGTTTC CAAGGGAACC AGGGCGTATC TCTTCATAGC CTTATGCAGT TGCTCTCCAG
   61 CGGTTCCATC TTCCAGCGGA TAGAATGGCG CCGGGCCTTT CTTTATGTTT TTGGCGTCTT
  121 CCATGGATCG GTCTCCAGCG TGACTTTCTT GACTCCCTCA AAGTCTTAGG CCAAAGGAAG
  181 CTTGCTTCAA GCAAGGCTGC TTCAGTGCTG TTGTGTGACA GAGCACTGGC CCCTGGCATT
  241 CAAATCTGAG CTTGCTCTTG TGGGCTCCTC CCCCACGACA GCATCACTAC CACCAGGTAT
  301 CCAACATCTG CCTCCTGGGT TGGGTCCAGT AAAGATGGGC GGGGCTGTGG TGGGCAAAGC
  361 CACACGGATT AAATGATTGG GGGGGGGGGC CAGTTCTGCT CATGCTTAAT GTCCAACTTC
  421 CTTCGAGGCT GTTCCTCTAA ACTCGAGAAC TGGTACAGCG GCAAAGCAGT TTACAGGGTT
  481 TTGATCAACT TTTATTTTCT TCATTAACTT CTCTTTCCCC TCATTAACTT CTATTGAAAA
  541 ACCACAAGCC AACTTGGTCA CATTGTTTTT AACCAAAAAT ACTGTGACTT GGAAATCGAG
  601 TCGCCTGCGG CTGAGCTGAG TTTTGGACAT CTGGAGTTCA CCACCTCGCT TTCCCTATGA
  661 GACACACAGG TGAGGTTTCG TTATGGCCAG CATGAGGGGC TGGCGGTCTC TGGAAAGCCT
  721 CGGGGGGGGG GGGGGGCTGC ATTCCTGTGT CTCAAAGCCA GCACACTCAA ACATGACTCC
  781 TCGGGTCACT ATTTTTGGAC ACCAGCTAGA TTGCTCAATA TGATACTGGT TGTTTTTAAA
  841 GGGATCCAAA AAGGGCTTTG TGTGGGTGAA AATGTGGTTG TTGGAATTGC TTCAACTTCT
  901 GCATTCCCCC GATTTTTGTT TTTCTCACTT TGTGGCCCTG GAGGTTACTG CATCCCTTCT
  961 GGGGTATGAA AAAGCCATGG GAACAGCCCT CAAGACACAG ACCAGAAATT AACTCAGCTT
 1021 TCTGCTGTGG GCCTGCCCAG GACATTCCAA AGAACCAGAA GAACTTGTAA ACCAGGTATT
 1081 TTATCAAAAA AGCTTAAGTT CTACGGGAAG TGAGTCAGGA AGGATGAAAA GTTCCAAGGC
1141 CAAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

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