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pGL3-AID R4 del.-14 (#RDB07868)

Reporter construct of mouse Aicda locus, position -9226 to -8003.

Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -8003.
Comment Nat. Immunol. (2010) 2, 148-154, Fig. 3c.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
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本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07868 pGL3-AID R4 del.-14 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3-AID R4 del.-14 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07868).

Reference section:

Tran, T.H., Nakata, M., Suzuki, K., Begum, N.A., Shinkura, R., Fagarasan, S., Honjo, T., Nagaoka, H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07868_A4Gi.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07868_A4Gia.seq check
>07868_07868_A4Gi_pGL4-4174F_D04_10.ab1
    1 ATTCAAGGGC CAAGAAACAT TTCTCTATCG ATAGGTACCC ACCACTCTCG GCTGTTCTGG
   61 AACTCTCTGT AACCTCGGGC TGTATGAGTG TATGTGAGTG TGTGTATATG GGTGTGGGGG
121 GGGTGAGAG
//
Primer: Luc_R
Sequence file: RDB07868_A4Gib.seq check
>07868_07868_A4Gi_Luc_R_D02_11.ab1
    1 GTCTGTTCAG GACAGGGCGT ATCTCTTCAT AGCCTTATGC AGTTGCTCTC CAGCGGTTCC
   61 ATCTTCCAGC GGATAGAATG GCGCCGGGCC TTTCTTTATG TTTTTGGCGT CTTCCATGGA
  121 TCGGTCTCCA GCGTGACTTT CTTGACTCCC TCAAAGTCTT AGGCCAAAGG AAGCTTGCTT
  181 CAAGCAAGGC TGCTTCAGTG CTGTTGTGTG ACAGAGCACT GGCCCCTGGC ATTCAAATCT
  241 GAGCTTGCTC TTGTGGGCTC CTCCCCCACG ACAGCATCAC TACCACCAGG TATCCAACAT
  301 CTGCCTCCTG GGTTGGGTCC AGTAAAGATG GGCGGGGCTG TGGTGGGCAA AGCCACACGG
  361 ATTAAATGAT TGGGGGGGGG GGCCAGTTCT GCTCATGCTT AATGTCCAAC TTCCTTCGAG
  421 GCTGTTCCTC TAAACTCGAG ACCACAAGCC AACTTGGTCA CATTGTTTTT AACCAAAAAT
  481 ACTGTGACTT GGAAATCGAG TCGCCTGCGG CTGAGCTGAG TTTTGGACAT CTGGAGTTCA
  541 CCACCTCGCT TTCCCTATGA GACACACAGG TGAGGTTTCG TTATGGCCAG CATGAGGGGC
601 TGGCGGTCTC TGGAAAGCCT CGGGGGGGGG GGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]