Resource data sheet
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pGL3-AID R4 del.-13

Reporter construct of mouse Aicda locus, position -9226 to -8223.

Catalog number RDB07867
Resource name pGL3-AID R4 del.-13
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -8223.
Nat. Immunol. (2010) 2, 148-154, Fig. 3c.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07867 pGL3-AID R4 del.-13 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07867_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB07867_A4Gia.seq
>07867_07867_A4Gi_pGL4-4174F_C04_07.ab1
    1 GTTAGGGGTC CAGAACATTT CTCTATCGAT AGGTACCCAC CACTCTCGGC TGTTCTGGAA
   61 CTCTCTGTAA CCTCGGGCTG TATGAGTGTA TGTGAGTGTG TGTATATGGG TGTGGGGGGG
121 GTGAGAGT
//
Primer: Luc_R RDB07867_A4Gib.seq
>07867_07867_A4Gi_Luc_R_B02_05.ab1
    1 GACTGTTCAG GGACCAGGGC GTATCTCTTC ATAGCCTTAT GCAGTTGCTC TCCAGCGGTT
   61 CCATCTTCCA GCGGATAGAA TGGCGCCGGG CCTTTCTTTA TGTTTTTGGC GTCTTCCATG
  121 GATCGGTCTC CAGCGTGACT TTCTTGACTC CCTCAAAGTC TTAGGCCAAA GGAAGCTTGC
  181 TTCAAGCAAG GCTGCTTCAG TGCTGTTGTG TGACAGAGCA CTGGCCCCTG GCATTCAAAT
  241 CTGAGCTTGC TCTTGTGGGC TCCTCCCCCA CGACAGCATC ACTACCACCA GGTATCCAAC
  301 ATCTGCCTCC TGGGTTGGGT CCAGTAAAGA TGGGCGGGGC TGTGGTGGGC AAAGCCACAC
  361 GGATTAAATG ATTGGGGGGG GGGGCCAGTT CTGCTCATGC TTAATGTCCA ACTTCCTTCG
  421 AGGCTGTTCC TCTAAACTCG AGCAGCACAC TCAGACATGA CTCCTCGGGT CACTATTTTT
  481 GGACACCAGC TAGATTGCTC AATATGATAC TGGTTGTTTT TAAAGGGATC CAAAAAGGGC
  541 TTTGTGTGGG TGAAAATGTG GTTGTTGGAT TGCTTCAACT TCTGCATTCC CCGATTTTTG
  601 TTTTTCTCAC TTTGTGGCCC TGGAGGTTAC TGCATCCCTC TGGGGTATGA AAAGCCATGG
  661 GACAGCCCTC AGACACAGAC CAGAATTACT CAGCTTCTGC TGTTGGGCTG CCCAGGACAT
  721 TTCAAAGACC AGAGAACTGT AAACAGGTAT TTATCAAAAG CTTAGGTCTA CGGGGAGGTG
  781 GAGGCAGGAG GATTGAGAGT TCAAGGCCAT CTTCTGCCTC GTGTTGAATT CAAGGCCAGC
  841 AGGCAGGTTT GAAAGAAAAA AAAGAATTTT CAAATGGAAT ACTATGAAAT TAAAGGCTTC
  901 AAACTGAGTG TGGTGACTAA TCCTAGCCAG CCTGGAGGTT GAGGCAGGAG GATTGTCAGA
  961 GTTGGGAAAC CACCCTTGAG CTCCATCCCC AGGACCAACA TGGGTAGGGG GGGGAGGTGA
 1021 ACCCAAATTC ATTGCCAGTT GGTCCCACTT GACCTCCTAC GCTCATTACA CACACACACC
 1081 TCATACCACA CCACCCATAA TACCCACTAG ACCACACTCA TACACCACCC CGTACCGCAC
1141 CACCTCTTAA CCCAACCACA CCCCAAACCG CATCGTACTG ACACGTTCGA CTAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

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