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pGL3-AID R4 del.-06

Reporter construct of mouse Aicda locus, position -8282 to -7585.

Catalog number RDB07860
Resource name pGL3-AID R4 del.-06
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -8282 to -7585.
Nat. Immunol. (2010) 2, 148-154, Fig. 3b.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07860 pGL3-AID R4 del.-06 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07860_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB07860_A4Gia.seq
>07860_07860_A4Gi_pGL4-4174F_E01_13.ab1
    1 AATAGGTCAG ACATTTCTCT ATCGATAGGT ACCATTGAGC AATCTAGCTG GTGTCCAAAA
   61 ATAGTGACCC GAGGAGTCAT GTCTGAGTGT GCTGGCTCTG AGACACAGGA ATGCAGCCCC
  121 CCCCCCCCCC CGAGGCTTTC CAGAGACCGC CAGCCCCTCA TGCTGGCCAT AACGAAACCT
  181 CACCTGTGTG TCTCATAGGG AAAGCGAGGT GGTGAACTCC AGATGTCCAA AACTCAGCTC
  241 AGCCGCAGGC GACTCGATTT CCAAGTCACA GTATTTTTGG TTAAAAACAA TGTGACCAAG
  301 TTGGCTTGTG GTTTTTCAAT AGAAGTTAAT GAGGGGAAAG AGAAGTTAAT GAAGAAAATA
  361 AAAGTTGATC AAAACCCTGT AAACTGCTTT GCCGCTGTAC CAGTTCCAAA TTTCCCGGGA
  421 CTGGAAAATG GTTTTTGTAA CTAAGTGAGA TCTCTTTCCT TCCTTTAGAA GAAGGCAAAT
  481 GAGAGAATTT GGCTTTCTTA CAAATCAAGG GTTTTGTATT TGTTCCTATG GCATGTGTAC
  541 GGCATCTGTA AGAGAGGGTG TGGGCGTGCG TGTGCGTGTG CTACAGCTCG AGTTTAGAGG
  601 AACAGCCTCG AAGGAAGTTG GACATTAAGC ATGAGCAGAA CTGGCCCCCC CCCCCAATCA
  661 TTTAATCCGT GTGGCTTTGC CCACCACAGC CCCGCCCATC TTTACTGGAC CCAACCCAGG
  721 AGGCAGATGT TGGATACCTG GTGGTAGTGA TGCTGTCGTG GGGGAGGAGC CCACAAGAGC
  781 AAGCTCAGAT TTGAATGCCA GGGGCCAGTG CTCTGTCACA CAACAGCACT GAAGCAGCCT
  841 TGCTTGAAGC AAGCTTCCTT TGGCCTAAGA CTTTGAGGGA GTCAAGAAAG TCACGCTGGA
  901 GACCGATCCA TGGAAGACGC CAAAAACATA AAGAAAGGCC CGGCGCCATT CTATCCGCTG
  961 GAAGATGGAA CCGCTGGAGA GCAACTGCAT AAAGGCTATG AAGAGATACG CCCTGGTTTC
 1021 CTGGAACAAT TGCTTTTACA GATGCACATA TCGAGGTGGA CATCACTTTA CGCTGAGTAC
 1081 TTCGAATGTC GTTTCGGTTG GCAGAAGCTA TGGAAACCGA TATGGCCTGG AATTACAAAT
1141 CACAGGAAGT CGGACGTATT GCCAGGGTGA AAAACT
//
Primer: Luc_R RDB07860_A4Gib.seq
>07860_07860_A4Gi_Luc_R_F01_16.ab1
    1 CTTTGATCAG GACAGGCGTA TCTCTTCATA GCCTTATGCA GTTGCTCTCC AGCGGTTCCA
   61 TCTTCCAGCG GAAAGAATGG CGCCGGGCCT TTCTTTATGT TTTTGGCGTC TTCCATGGAT
  121 CGGTCTCCAG CGTGACTTTC TTGACTCCCT CAAAGTCTTA GGCCAAAGGA AGCTTGCTTC
  181 AAGCAAGGCT GCTTCAGTGC TGTTGTGTGA CAGAGCACTG GCCCCTGGCA TTCAAATCTG
  241 AGCTTGCTCT TGTGGGCTCC TCCCCCACGA CAGCATCACT ACCACCAGGT ATCCAACATC
  301 TGCCTCCTGG GTTGGGTCCA GTAAAGATGG GCGGGGCTGT GGTGGGCAAA GCCACACGGA
  361 TTAAATGATT GGGGGGGGGG GCCAGTTCTG CTCATGCTTA ATGTCCAACT TCCTTCGAGG
  421 CTGTTCCTCT AAACTCGAGC TGTAGCACAC GCACACGCAC GCCCACACCC TCTCTTACAG
  481 ATGCCGTACA CATGCCATAG GAACAAATAC AAAACCCTTG ATTTGTAAGA AAGCCAAATT
  541 CTCTCATTTG CCTTCTTCTA AAGGAAGGAA AGAGATCTCA CTTAGTTACA AAAACCATTT
  601 TCCAGTCCCG GGAAATTTGG AACTGGTACA GCGGCAAAGC AGTTTACAGG GTTTTGATCA
  661 ACTTTTATTT TCTTCATTAA CTTCTCTTTC CCCTCATTAA CTTCTATTGA AAAACCACAA
  721 GCCAACTTGG TCACATTGTT TTTAACCAAA AATACTGTGA CTTGGAAATC GAGTCGCCTG
  781 CGGCTGAGCT GAGTTTTGGA CATCTGGAGT TCACCACCTC GCTTTCCCTA TGAGACACAC
  841 AGGTGAGGTT TCGTTATGGC CAGCATGAGG GGCTGGCGGT CTCTGGAAAG CCTCGGGGGG
  901 GGGGGGGGGG GCTTGCATTT CCTGGTGTCT CAAGAGCCAG CACACTTCAG ACATGAACTC
  961 CTCGGGTTCA CTATTTTTTG GGACACCAGC TTAGATTGGC TCAATGGTTA CCCTATCCGA
 1021 ATAGAAGAAA TGGTTCTGGG CCACCTTGCA ACTTGCACTG GGGACAGCCT ATTTTGCTTA
 1081 GGTTTGTTTG TTTCGGTTTG TTTTTGATGG AGAGCGTATG TTAAGTACTA TTCGGAATTC
1141 ACCACAAAAA ACCTAACAC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

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