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pGL3-AID delR2 + R4

Reporter construct of mouse Aicda locus, position -9226 to -7585 plus +1202 to +1789.

Catalog number RDB07847
Resource name pGL3-AID delR2 + R4
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -7585 plus +1202 to +1789.
Nat. Immunol. (2010) 2, 148-154, Fig. 2d.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07847 pGL3-AID delR2 + R4 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07847_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB07847_A4Gia.seq
>07847_07847_A4Gi_pGL4-4174F_A04_01.ab1
    1 ATTGGGGTCC GGAAAAATTG TGGTCTATCG ATAGGTACCC ACCACTCTCG GCTGTTCTGG
61 AACTCTCTGT AACCTCGGGC GGTATGAGTG TATGTGAGTG TGTGTATATG G
//
Primer: pGL3_RV5F RDB07847_A4Gib.seq
>07847_07847_A4Gi_pGL3_Rv5F_G03_21.ab1
    1 ACTTTCTCAA ATGTGGTAAA TCGATAAGGA TCCACTCTCC CTCACCCCTA TCAGGGACCA
   61 CATTGAAGAC CTCACACATG CTAGACAAGT AATCTGCCAC TTAATTACAT CCTGAGCCCT
  121 CAAAAAGCAA ACAGACAGAC AGACAGACAG ACAGACAGAC AGACAAACAA ACAAATGTTC
  181 ACAGGAGGCA GGCAGACAGC CTGAGCTGCT TCTGGGTTTA TAGTGAATTT TGAAACCAAA
  241 TCTGAGATCT ATGTCCTGAT GGAGAAGGGT CCGAGAGAAA TGCATGAGCA TGGCAAAATG
  301 CAAAGCAAAG ACGAGGCTGA GATTCAGGGA GAAGCAAACA AGACAGTGGA GAGACACAGG
  361 ATGGCACGGC ACGGACTGGA GCAAGGGCAG CGAGTAACTC AAGGCAGCCC TGCTACTAGG
  421 CTGGGATTAT TTTTAACCCC TTGAGTCTGG TTTGCATTGC TGGGGAAGCA GCTAAGGTTC
  481 TGCCTCAAGG AGCACAGCTG TCTCAGCAGC TGGCGATCTA CAGGTTTGGG ACACCACCTA
  541 GCAAAGTCCT CCTTACCGGG AGGGACATCC CGAGGAGAGG GAGCTGGAAA TAGGCTCCTA
  601 GCTAGAGTTG AGGGGGGATC CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT
  661 CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA
  721 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG
  781 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA
  841 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC
  901 AGGAACCGTA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC
  961 ATCACAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
 1021 GCGTTTCCCC TGGAAGCTCC CCTCGTGCGC TCTCTGTCGA CCTGCCGCTT ACGATTACCT
 1081 GTCGCTTTCT CCCTTCGGAA GCGTGCGCTT TCTCTAGCTC AGCCTGTAAG GTATTCTTCA
1141 GTTCCGAGTG TTAG
//
Primer: RDB07847_A4Gic.seq
>07847_07847_A4Gi_pGL3_Rv6R_F03_18.ab1
    1 GACATCGTCA GCGGATAGAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATGG
   61 ATCGGTCTCC AGCGTGACTT TCTTGACTCC CTCAAAGTCT TAGGCCAAAG GAAGCTTGCT
  121 TCAAGCAAGG CTGCTTCAGT GCTGTTGTGT GACAGAGCAC TGGCCCCTGG CATTCAAATC
  181 TGAGCTTGCT CTTGTGGGCT CCTCCCCCAC GACAGCATCA CTACCACCAG GTATCCAACA
  241 TCTGCCTCCT GGGTTGGGTC CAGTAAAGAT GGGCGGGGCT GTGGTGGGCA AAGCCACACG
  301 GATTAAATGA TTGGGGGGGG GGGCCAGTTC TGCTCATGCT TAATGTCCAA CTTCCTTCGA
  361 GGCTGTTCCT CTAAACTCGA GCTGTAGCAC ACGCACACGC ACGCCCACAC CCTCTCTTAC
  421 AGATGCCGTA CACATGCCAT AGGAACAAAT ACAAAACCCT TGATTTGTAA GAAAGCCAAA
  481 TTCTCTCATT TGCCTTCTTC TAAAGGAAGG AAAGAGATCT CACTTAGTTA CAAAAACCAT
  541 TTTCCAGTCC CGGGAAATTT GGAACTGGTA CAGCGGCAAA GCAGTTTACA GGGTTTTGAT
  601 CAACTTTTAT TTTCTTCATT AACTTCTCTT TCCCCTCATT AACTTCTATT GAAAAACCAC
  661 AAGCCAACTT GGTCACATTG TTTTTAACCA AAAATACTGT GACTTGGAAA TCGAGTCGCC
  721 TGCGGCTGAG CTGAGTTTTG GACATCTGGA GTTCACCACC TCGCTTTCCC TATGAGACAC
  781 ACAGGTGAGG TTTCGTTATG GCCAGCATGA GGGGCTGGCG GTCTCTGGAA AGCCTCGGGG
  841 GGGGGGGGGG GCTGCATCCC TTGTGTCTCA AAGCCAGCAC ACTCAAAACA TGACTCCTCG
  901 GGTCACTATT TTTTGAACAC CAGCCTAAGA TTGCTTCAAA TATGAATACT GGTTGTTTTA
  961 AAGGGAATCC AAAAAGGCTT GGTGTGGGTG AAATGTGTGT GATTGCTTCA ACTCTGCATC
 1021 CCGATTTTTG TTTTTTCTCA CTTTGTGCTG GAAGGTTACT GGCATTCCCT GGATGAAAGC
1081 AATGGACGCT GACGACGAAT ACTCAGCTTC GCTGTTGACT GGCCTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl