RDB07847 - pGL3-AID delR2 + R4

Reporter construct of mouse Aicda locus, position -9226 to -7585 plus +1202 to +1789.

Distribution information

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
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References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07847	pGL3-AID delR2 + R4	DNA solution

original	Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: pGL4-4174F Sequence file: RDB07847_A4Gia.seq
>07847_07847_A4Gi_pGL4-4174F_A04_01.ab1 1 ATTGGGGTCC GGAAAAATTG TGGTCTATCG ATAGGTACCC ACCACTCTCG GCTGTTCTGG 61 AACTCTCTGT AACCTCGGGC GGTATGAGTG TATGTGAGTG TGTGTATATG G //
Primer: pGL3_RV5F Sequence file: RDB07847_A4Gib.seq
>07847_07847_A4Gi_pGL3_Rv5F_G03_21.ab1 1 ACTTTCTCAA ATGTGGTAAA TCGATAAGGA TCCACTCTCC CTCACCCCTA TCAGGGACCA 61 CATTGAAGAC CTCACACATG CTAGACAAGT AATCTGCCAC TTAATTACAT CCTGAGCCCT 121 CAAAAAGCAA ACAGACAGAC AGACAGACAG ACAGACAGAC AGACAAACAA ACAAATGTTC 181 ACAGGAGGCA GGCAGACAGC CTGAGCTGCT TCTGGGTTTA TAGTGAATTT TGAAACCAAA 241 TCTGAGATCT ATGTCCTGAT GGAGAAGGGT CCGAGAGAAA TGCATGAGCA TGGCAAAATG 301 CAAAGCAAAG ACGAGGCTGA GATTCAGGGA GAAGCAAACA AGACAGTGGA GAGACACAGG 361 ATGGCACGGC ACGGACTGGA GCAAGGGCAG CGAGTAACTC AAGGCAGCCC TGCTACTAGG 421 CTGGGATTAT TTTTAACCCC TTGAGTCTGG TTTGCATTGC TGGGGAAGCA GCTAAGGTTC 481 TGCCTCAAGG AGCACAGCTG TCTCAGCAGC TGGCGATCTA CAGGTTTGGG ACACCACCTA 541 GCAAAGTCCT CCTTACCGGG AGGGACATCC CGAGGAGAGG GAGCTGGAAA TAGGCTCCTA 601 GCTAGAGTTG AGGGGGGATC CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT 661 CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA 721 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG 781 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 841 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 901 AGGAACCGTA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 961 ATCACAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 1021 GCGTTTCCCC TGGAAGCTCC CCTCGTGCGC TCTCTGTCGA CCTGCCGCTT ACGATTACCT 1081 GTCGCTTTCT CCCTTCGGAA GCGTGCGCTT TCTCTAGCTC AGCCTGTAAG GTATTCTTCA 1141 GTTCCGAGTG TTAG //
Primer: Sequence file: RDB07847_A4Gic.seq
>07847_07847_A4Gi_pGL3_Rv6R_F03_18.ab1 1 GACATCGTCA GCGGATAGAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATGG 61 ATCGGTCTCC AGCGTGACTT TCTTGACTCC CTCAAAGTCT TAGGCCAAAG GAAGCTTGCT 121 TCAAGCAAGG CTGCTTCAGT GCTGTTGTGT GACAGAGCAC TGGCCCCTGG CATTCAAATC 181 TGAGCTTGCT CTTGTGGGCT CCTCCCCCAC GACAGCATCA CTACCACCAG GTATCCAACA 241 TCTGCCTCCT GGGTTGGGTC CAGTAAAGAT GGGCGGGGCT GTGGTGGGCA AAGCCACACG 301 GATTAAATGA TTGGGGGGGG GGGCCAGTTC TGCTCATGCT TAATGTCCAA CTTCCTTCGA 361 GGCTGTTCCT CTAAACTCGA GCTGTAGCAC ACGCACACGC ACGCCCACAC CCTCTCTTAC 421 AGATGCCGTA CACATGCCAT AGGAACAAAT ACAAAACCCT TGATTTGTAA GAAAGCCAAA 481 TTCTCTCATT TGCCTTCTTC TAAAGGAAGG AAAGAGATCT CACTTAGTTA CAAAAACCAT 541 TTTCCAGTCC CGGGAAATTT GGAACTGGTA CAGCGGCAAA GCAGTTTACA GGGTTTTGAT 601 CAACTTTTAT TTTCTTCATT AACTTCTCTT TCCCCTCATT AACTTCTATT GAAAAACCAC 661 AAGCCAACTT GGTCACATTG TTTTTAACCA AAAATACTGT GACTTGGAAA TCGAGTCGCC 721 TGCGGCTGAG CTGAGTTTTG GACATCTGGA GTTCACCACC TCGCTTTCCC TATGAGACAC 781 ACAGGTGAGG TTTCGTTATG GCCAGCATGA GGGGCTGGCG GTCTCTGGAA AGCCTCGGGG 841 GGGGGGGGGG GCTGCATCCC TTGTGTCTCA AAGCCAGCAC ACTCAAAACA TGACTCCTCG 901 GGTCACTATT TTTTGAACAC CAGCCTAAGA TTGCTTCAAA TATGAATACT GGTTGTTTTA 961 AAGGGAATCC AAAAAGGCTT GGTGTGGGTG AAATGTGTGT GATTGCTTCA ACTCTGCATC 1021 CCGATTTTTG TTTTTTCTCA CTTTGTGCTG GAAGGTTACT GGCATTCCCT GGATGAAAGC 1081 AATGGACGCT GACGACGAAT ACTCAGCTTC GCTGTTGACT GGCCTG //