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pGL3-AID R2 del.-02

Reporter construct of mouse Aicda locus, position +384 to +1789.

Catalog number RDB07831
Resource name pGL3-AID R2 del.-02
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, +384 to +1789.
Nat. Immunol. (2010) 2, 148-154, Fig. 2b.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07831 pGL3-AID R2 del.-02 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07831_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ME-1290Rv RDB07831_A4Gia.seq
>07831_07831_A4Gi_ME_1290Rv_H11_23.ab1
    1 AGGGCAATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
   61 TAAAACCTCT ACAAATGTGG TAAAATCGAT AAGGATCCAA GAGGTCTAGC AACTCCTTAC
  121 TAAAAACAGT AGTGGTTATT TTCACAATTA TTTGGCAAAT ATCCAACATC TTAAGACTCG
  181 CATGGGGAGT CTTTACAGGA ATTATTTAGT TATAGCAAGA AGATTTGTAC TTCTCAAAAA
  241 AAAAAAAAAA CTAAACATTT GAAATGAATT GCTTGCAACT CATTACAATG GTGTCTATTG
  301 AAGGAAAGAA TTTCATTAAA ACAGGCAATT TAGTGTTATA GACTCAACTG TTAAACACTT
  361 GGTGACATTT TTACTGTTTA ATTCATCTAT GCAGAGATTT CTTAGCTTCT TGAAAGCTTT
  421 TATATGCAGC TCATGATGAG CCATTATCAG AAATTTCTCT CTTGATTTTT ACATTTATTG
  481 CCAGTGTGTG AGTCACTATG CCTAAAGCCC ATACACTTGA GCTCACTTCC GTTTGGCTAT
  541 GAGGTTTAGA ATATGGAGTT AATATAGCTA ATGGTAGCAG GGTGTTCTTC AAATTCCAGA
  601 TTTTTCCTTT CTTGTCTTCC TTCTTTCTTT TTGTTACCCT TCTCCTACCC CCTCTTCTTC
  661 TCCCCCTCCT CCTCTTCCTC CCTCCTCCCC ATCTCTTCCC CTCCTCTTCT CCCTCCTCCC
  721 CCTCCTCTTC CCCTTCTCCA CTTCCCCCTC TTCCTCCTCT TCCTCCTCCT TCTCCTGCTC
  781 CCCATCTCTT TCCCTCCTCT TCTCCCTCCT CCTCCTCTTT GGCCCCTTCT CTCTTCCCCC
  841 CTCCTCCTCT CCCTTTTCCT TCTCCTCTTC TCTCCCTCTC TTCCCGTTTC TCCTCCAACT
  901 CTCCTTCACC CTATCAGGGA CCACATTGAG ACTTCACACA TGCTAGACAA GTAATCTGGC
  961 ACATAATTAC ATCCTGGAGT CTTGAAAGCA ACAGACTGAA CGACGACGAC GGCTGGACGA
 1021 CAAACCAACA TGTTTCCAAG AGCAAGCACA CGCCTGAACT GCTTCGGTTT AATGGAATTG
1081 ACCACCTGAA TAATGTCTGA GGGAGTTCCA GAAAAGTCCT GACATGGCCA AG
//
Primer: pGL3_RV4 RDB07831_A4Gib.seq
>07831_07831_A4Gi_Rv4_A12_03.ab1
    1 GCAGGACTGA TGGGTTGAGG CTCTCAAGGG CATCGGTCGA CGGATCCCCC CTCAACTCTA
   61 GCTAGGAGCC TATTTCCAGC TCCCTCTCCT CGGGATGTCC CTCCCGGTAA GGAGGACTTT
  121 GCTAGGTGGT GTCCCAAACC TGTAGATCGC CAGCTGCTGA GACAGCTGTG CTCCTTGAGG
  181 CAGAACCTTA GCTGCTTCCC CAGCAATGCA AACCAGACTC AAGGGGTTAA AAATAATCCC
  241 AGCCTAGTAG CAGGGCTGCC TTGAGTTACT CGCTGCCCTT GCTCCAGTCC GTGCCGTGCC
  301 ATCCTGTGTC TCTCCACTGT CTTGTTTGCT TCTCCCTGAA TCTCAGCCTC GTCTTTGCTT
  361 TGCATTTTGC CATGCTCATG CATTTCTCTC GGACCCTTCT CCATCAGGAC ATAGATCTCA
  421 GATTTGGTTT CAAAATTCAC TATAAACCCA GAAGCAGCTC AGGCTGTCTG CCTGCCTCCT
  481 GTGAACATTT GTTTGTTTGT CTGTCTGTCT GTCTGTCTGT CTGTCTGTCT GTTTGCTTTT
  541 TGAGGGCTCA GGATGTAATT AAGTGGCAGA TTACTTGTCT AGCATGTGTG AGGTCTTCAA
  601 TGTGGTCCCT GATAGGGGTG AGGGAGAGTG GGAGGAGGAA AGGGGGAGGA GAGGGGGGGG
  661 ATGGAGTCGG ACGATGAGGA TGAGGGCCAT GAAGAGGGTC AGCTCCATCC GGTGCAAGAG
  721 GAGGATGAGG AGGGAGAAGA GGAGGGAAAG AGATGGGGAG CAGGAGAAGG AGGAGGAAGA
  781 GGAGGAAGAG GGGGAAGAGG AGAAGGGGAA GAGGAGGGGG AGGAGGGAGA AAGAGGAGGG
  841 GAAGAGATGG GGAGGAGGGA GGAAGAGGAG GAGGGGGAGA AGAAGAGGGG GTAGGAGAAG
901 GGTACAAAAA AGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

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