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pGL3-AID R2 del.-01

Reporter construct of mouse Aicda locus, position +231 to +1789.

Catalog number RDB07830
Resource name pGL3-AID R2 del.-01
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, +231 to +1789.
Nat. Immunol. (2010) 2, 148-154, Fig. 2b.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07830 pGL3-AID R2 del.-01 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07830_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ME-1290Rv RDB07830_A4Gia.seq
>07830_07830_A4Gi_ME_1290Rv_F11_17.ab1
    1 ATGGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
   61 AAAACCTCTA CAAATGTGGT AAAATCGATA AGGATCCACC TAACTCTGAC CACGAAGTTC
  121 TTCCCTTCCC CCGGTTTCTC TCTTCTCTGT GTTGCTAGAG ATAGGAAGCC TTGACTTGTC
  181 CTGAGATTTG GGCAGAGCTA GAGCCGGCTT GTGGTAATAA CAGTGAAGCC TTAGAGGCCC
  241 GCGCCACAAA GAGGTCGTAG CAACTCCTTA CTAAAAACAG TAGTGGTTAT TTTCACAATT
  301 ATTTGGCAAA TATCCAACAT CTTAAGACTC GCATGGGGAG TCTTTACAGG AATTATTTAG
  361 TTATAGCAAG AAGATTTGTA CTTCTCAAAA AAAAAAAAAA CTAAACATTT GAGATGAATT
  421 GCTTGCAACT CATTACAATG GTGTCTATTG AAGGAGAGAA TTTCATTAAG ACAGGCAATT
  481 TAGTGTTATA GACTCAACTG TTAGACACTT GGTGACATTT TTACTGTTTA ATTCATCTAT
  541 GCAAAGATTT CTTAGCTTCT TGAAAGCTTT TATATGCAGC TCATGATGAG CCATTATCAG
  601 AAATTTCTCT CTTGATTTTT ACATTTATTG CCAGTGTGTG AGTCACTATG CCTAAAGCCC
  661 ATACACTTGA GCTCACTTCC GTTTGGCTAT GAGGTTTAGA ATATGGAGTT AATATAGCTA
  721 ATGGTAGCAG GGTGTTCTTC AGATTCCAGA TTTTTCCTTT CTTGTCTTCC TTCTTTCTTT
  781 TTGTTACCCT TCTCCTACCC CCTCTTCTTC TCCCCCTCCT CCTCTTCCTC CCTCCTCCCC
  841 ATCTCTTCCC TTCCTCTTCT CCCTCCTCCC CCTCCTCTTC CCCTTCTCCA CTTCCCCCTC
  901 TTCCTCCTCT TCCTCCTCCT TCTCCTGCTC CCCATCTCTT TCCCTCCTCT TCTCCCTCCT
  961 CCTCCTCCTT TTGCCCCTTC TCCTCTTCCC CCTCCTCCTC TTCCTTTTCC CTTCTCCTCC
 1021 TTCTCCTCCC CCTCTCCTCC CCCTTTCTCT CCCACTCTCC TCACCCCCTA TCAGCACCCA
 1081 CATTGAAGAC CCTCACCACA TGGCTTAGAC AAGTAATCTG CCCACTTAAC TTACATCCTT
1141 GAAGTCCTTG AATGCAACAG ACTGAACGGC AACGGGGAAA CG
//
Primer: pGL3_RV4 RDB07830_A4Gib.seq
>07830_07830_A4Gi_Rv4_G11_20.ab1
    1 GCAGGACGAT GGGTTGAGGC TCTCAAGGGC ATCGGTCGAC GGATCCCCCC TCAACTCTAG
   61 CTAGGAGCCT ATTTCCAGCT CCCTCTCCTC GGGATGTCCC TCCCGGTAAG GAGGACTTTG
  121 CTAGGTGGTG TCCCAAACCT GTAGATCGCC AGCTGCTGAG ACAGCTGTGC TCCTTGAGGC
  181 AGAACCTTAG CTGCTTCCCC AGCAATGCAA ACCAGACTCA AGGGGTTAAA AATAATCCCA
  241 GCCTAGTAGC AGGGCTGCCT TGAGTTACTC GCTGCCCTTG CTCCAGTCCG TGCCGTGCCA
  301 TCCTGTGTCT CTCCACTGTC TTGTTTGCTT CTCCCTGAAT CTCAGCCTCG TCTTTGCTTT
  361 GCATTTTGCC ATGCTCATGC ATTTCTCTCG GACCCTTCTC CATCAGGACA TAGATCTCAG
  421 ATTTGGTTTC AAAATTCACT ATAAACCCAG AAGCAGCTCA GGCTGTCTGC CTGCCTCCTG
  481 TGAACATTTG TTTGTTTGTC TGTCTGTCTG TCTGTCTGTC TGTCTGTCTG TTTGCTTTTT
  541 GAGGGCTCAG GATGTAATTA AGTGGCAGAT TACTTGTCTA GCATGTGTGA GGTCTTCAAT
  601 GTGGTCCCTG ATAGGGGTGA GGGAGAGTGG GAGGAGGAAA GGGGGAGGAG AGGGGGGATG
  661 AGTCGGCCGA TGCGGTTGAG AGCCTTGAAC ACAGTCAGCT CCTTCCAGGT GCACGAGGAG
  721 GAGGAGGAGG GAGAAGAGGA GGGAAAGAGA TGGGGAGCAG GAGAAGGAGG AGGAAAGAGG
  781 AGGAAGAGGG GGAAGAGGAG AAGGGGAAGA GGAGGGGGGA GGGAGGGGAG AAGAGGAGGG
841 GGATAGATGG GGAGTGGGAG AAACATTCAT ATTTGTTTAA TT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

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