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pGL3-AID R1 del.-04

Reporter construct of mouse Aicda locus, position -839 to +101.

Catalog number RDB07827
Resource name pGL3-AID R1 del.-04
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -839 to +101.
Nat. Immunol. (2010) 2, 148-154, Fig. 1a.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07827 pGL3-AID R1 del.-04 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07827_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB07827_A4Gia.seq
>07827_07827_A4Gi_pGL4-4174F_D11_11.ab1
    1 AGGAGGGGTC CAGAAATTTC TCTATCGATA GGTACCGAGC TCTTACGCGT GCTAGCCCGG
   61 GCTCGAGTGA TCAGGGTTCA GATCCCAGCA AGCACACATG GTGGCTTTCA ACTGCCTGCT
  121 GGTCCTCTGC AATAGAAGTA AGTGCTCTTA ATCACTGGGC CAGCTCTCCA GGCCTCCAGT
  181 AAGGTATTTT TAATGAGGAA AAGGAGTTCT TTTTTTTTTT AATACTTTTT GACACACACA
  241 CACACACAAA ATTAAAATAA ATCACTTTTT GGTGCAAGCA ACTAGTCTTT CTAGCTATCT
  301 TATAATGTCA TTTTAAAAAA AGAAAAATAT ATTAGAGAAT TGGGAGGCTA AAGTTCACTC
  361 TCTGGATGCT GTGGTGGTCA ACCCCCATCT CTACTGAGGC ATAAAACTGA GTGTAACAAA
  421 CGGAAGGAAC AGATACTGTA AGTTCAAGAA GCACAAGATG CAACTGAAGT GCCATTTAAG
  481 GCCACTTTAA GTCACTATGA CTGCTATCAT TCTTGTTATC ATAGTTTTAA AATTAGGAAG
  541 TATGCACAGA CCTTAGGTGT GATACCCGGG ACCACACACA CACACACACA CACACACTCA
  601 CAGAGCTCAT TATCATGATA CCAATGTGAG AAGTGTCCAG TGCTATTGTC TCCTGATCTT
  661 TGTTACCTGT GATACCTGGG CTGGCTTTTT AGAGGAACAG CCTCGAAGGA AGTTGGACAT
  721 TAAGCATGAG CAGAACTGGC CCCCCCCCCC AATCATTTAA TCCGTGTGGC TTTGCCCACC
  781 ACAGCCCCGC CCATCTTTAC TGGACCCAAC CCAGGAGGCA GATGTTGGAT ACCTGGTGGT
  841 AGTGATGCTG TCGTGGGGGA GGAGCCCACA AGAGCAAGCT CAGATTTGAA TGCCAGGGGC
  901 CAGTGCTCTG TCACACAACA GCACTGAAGC AGCCTTGCTT GAAGCAAGCT TCCTTTGGCC
  961 TAAGACTTTG AGGGAGTCAA GAAAGTCACG CTGGGAGACC GATCCATGGA AGACGCCCAA
 1021 AAACATAAAG AAAGGCCCGG GCGCCAATTC TATCCGCTGA GATGGGAAAC CGCTGGGAGA
 1081 GCACTGCATA AGCTTATGAG AGAATACGCC CTGGATCTGA CCATTGCTTT ACAGATGCAC
1141 AATAATTCGA AGGGTGGCA
//
Primer: Luc_R RDB07827_A4Gib.seq
>07827_07827_A4Gi_Luc_R_E11_14.ab1
    1 CATTGCTTCA GGGACCAGGG CGTATCTCTT CATAGCCTTA TGCAGTTGCT CTCCAGCGGT
   61 TCCATCTTCC AGCGGATAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT
  121 GGATCGGTCT CCAGCGTGAC TTTCTTGACT CCCTCAAAGT CTTAGGCCAA AGGAAGCTTG
  181 CTTCAAGCAA GGCTGCTTCA GTGCTGTTGT GTGACAGAGC ACTGGCCCCT GGCATTCAAA
  241 TCTGAGCTTG CTCTTGTGGG CTCCTCCCCC ACGACAGCAT CACTACCACC AGGTATCCAA
  301 CATCTGCCTC CTGGGTTGGG TCCAGTAAAG ATGGGCGGGG CTGTGGTGGG CAAAGCCACA
  361 CGGATTAAAT GATTGGGGGG GGGGGCCAGT TCTGCTCATG CTTAATGTCC AACTTCCTTC
  421 GAGGCTGTTC CTCTAAAAAG CCAGCCCAGG TATCACAGGT AACAAAGATC AGGAGACAAT
  481 AGCACTGGAC ACTTCTCACA TTGGTATCAT GATAATGAGC TCTGTGAGTG TGTGTGTGTG
  541 TGTGTGTGTG TGGTCCCGGG TATCACACCT AAGGTCTGTG CATACTTCCT AATTTTAAAA
  601 CTATGATAAC AAGAATGATA GCAGTCATAG TGACTTAAAG TGGCCTTAAA TGGCACTTCA
  661 GTTGCATCTT GTGCTTCTTG AACTTACAGT ATCTGTTCCT TCCGTTTGTT ACACTCAGTT
  721 TTATGCCTCA GTAGAGATGG GGGTTGACCA CCACAGCATC CAGAGAGTGA ACTTTAGCCT
  781 CCCAATTCTC TAATATATTT TTCTTTTTTT AAAATGACAT TATAAGATAG CTAGAAAGAC
  841 TAGTTGCTTG CACCAAAAAG TGATTTATTT TAATTTTGTG TGTGTGTGTG TGTCAAAAAG
  901 TATTAAAAAA AAAAAGAACT CCTTTTCCTC ATTAAAAATA CCTTACTGGG AGGGCCTGGA
  961 GAGCTGGCCC AGTGATTAAA GAGCACTTAC TTCTATTGCA GAGGACCAGC AGGCAGTTGA
 1021 AGCCACCATG GTGGTGCTTT GCTGGGATCT GACCCCTGAT CACTCGGAGG CCCGGACTAG
 1081 CACGGCGTAG GAGCTCGGCT ACTATCGATT AGAGAAATTG ATCCTGGGCA CCTGGCAACG
1141 TTGCACCTGG GGGGACAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl