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pGL4-phRAD51 (#RDB07813)

Promoter collection, Human RAD51 promoter

Alternative name U3215-2
Clone info. PCR amplified human RAD51 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 40693865 to 40695330(NC_000015.10 ); relatively -1309 to +157, where +1 corresponds to 1 nt of NM_002875.5.
Comment PCR cloning, forward primer: ccgtgcaggccttatatgat; reverse primer: ctggcttacgctccacttct. Entire sequence of promoter region has not been confirmed. Known difference (at least) : A to G substitution at 40,694,157nt of NC_000015.10.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human RAD51 genomic DNA
Depositor|Developer DNA Bank, |
 

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RDB07813 pGL4-phRAD51 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phRAD51 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07813).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07813_A7I6p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-136R
Sequence file: RDB07813_A7I6b.seq check
>07813_07813_A7I6_pGL4-136R_H01_08_ABI08.ab1
    1 TCCCAATGTT TTTGGCATCT TCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG
   61 GCCGCCGAGG CCAGATCTTG ATCTGGCTTA CGCTCCACTT CTCTACTCGC TTGCCCCAGC
  121 CTTCTGCGCA CAACCCAGAT GAGTTGCAGT TCCCAGCTGC ACGCCTCGGG AAGCGCCGCA
  181 CTCTCCTTAG GGCTCGGTCT CTGGCCGCTG CGCGCGGTCC GCCAGCGGCT TTCAGAATTC
  241 CCGCCAAACC CTCTCGCCCA GGGTCACGCC CGCGTCGACG TAACGTATCC CCGCCTCCCG
  301 GATCCGCCTT CTCCCCGCTT CCGCCCTGCG CGCTAACCCA AGACGGGAGC TCGAGAGCTT
  361 GATCCTGCGC GAGTTTACAG ACTGCCCTCT TCCCTTTTTG CGTAAGGACT AATTCAAACT
  421 TTATGCCGGG GGCGGTGGCT CACGCGTGTA GTCCCAGCTG CTCGGGAGGC CGAGGCGAGA
  481 GTATCGTCTG AGCCTAGGAG TTCGAGGCTA ACCACGGCAA CGCCCCTACT CCTACCCCAG
  541 TCCCTCGCGG GTGGGGCGAG GGATTTCAGG GGGCGGTGGG GGAAGACTTA ACCGAGTTGC
  601 CGTCTTCTGT TTACCAGATC GAAGACCACG ACTCCCAAGA GGTAATGCGT CGGCCCGGGA
  661 CTACATCTCC CGGCATGCAT CGCCGGCGGG TCCGCGAGTT CTCACCATCG CAGCTGGACT
  721 ATTAGCGGGG GCCCTAGCGC TCTTGTGGTT TGTTTCGGCA GTATCTGAAT GTCAGCTTTT
  781 GGCACTTCTG GTCGCTCTGA GCCTCCTACT CGCATTCCTG GAGCAAGGTG CTTTCCAGGC
  841 CTCATGGAGG TGGAGGAGCT GAGGAAGCTC GAGAAGATGG ATAGAAGTGG GAAATGGAGC
  901 TAGCGTACGG GAGAGGGAAA TGGCTGGAAT ATTCGTCAAT CTGTGGTGCT TTACTGGGAG
  961 ATTTGCAGGA GTTCAGAGGA ACGGGGGCAT TGAAATTTCA TAACCTAAAC CGTATCGTGG
 1021 TTTACTCAGA AAAGTTTTGA ACCCGTGATT TCCGGGTTTA AAGCTTGAGG TAAGGACCGG
1081 GGCGGCGGTG GTCACGGCAG TTAATCCTAG CAGGTTAGAG GTCTAAGGTC AAGG
//
Primer: pAxCALNL_F1
Sequence file: RDB07813_A7I6c.seq check
>07813_07813_A7I6_pAxCALNL_F1_A02_01_ABI08.ab1
    1 ATAATTTTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA
   61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC
  121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT
  181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT
  241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC
  301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG
  361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG
  421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG
  481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA
  541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC
  601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT
  661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA
  721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC
  781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG
  841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC
  901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGG
  961 TGAACATGCC GAAGCCGTGG GTGAAATGGC ACCCACGCTG AGGGATAGCG GGTGTCGGGG
 1021 ATGAATCTGC TTTGCCCGAG ATGGGGGTCG CGGCAATGGA CTGAATCGGA CACAAGCGGC
1081 TGCGGCTGCC GTTAGGGACT ACGCCCTTGG GGCCATC
//
Primer:
Sequence file: RDB07813_A7I6d.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles