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pGL4-phRAD51

Promoter collection, Human RAD51 promoter

Catalog number RDB07813
Resource name pGL4-phRAD51
Alternative name U3215-2
Clone info. PCR amplified human RAD51 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 40693865 to 40695330(NC_000015.10 ); relatively -1309 to +157, where +1 corresponds to 1 nt of NM_002875.5.
Comment PCR cloning, forward primer: ccgtgcaggccttatatgat; reverse primer: ctggcttacgctccacttct. Entire sequence of promoter region has not been confirmed. Known difference (at least) : A to G substitution at 40,694,157nt of NC_000015.10.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human RAD51 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human RAD51

          Reference sequence
            

          Distribution information

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          RDB07813 pGL4-phRAD51 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07813_A7I6p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-136R
          Sequence file: RDB07813_A7I6b.seq check
          >07813_07813_A7I6_pGL4-136R_H01_08_ABI08.ab1
              1 TCCCAATGTT TTTGGCATCT TCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG
             61 GCCGCCGAGG CCAGATCTTG ATCTGGCTTA CGCTCCACTT CTCTACTCGC TTGCCCCAGC
            121 CTTCTGCGCA CAACCCAGAT GAGTTGCAGT TCCCAGCTGC ACGCCTCGGG AAGCGCCGCA
            181 CTCTCCTTAG GGCTCGGTCT CTGGCCGCTG CGCGCGGTCC GCCAGCGGCT TTCAGAATTC
            241 CCGCCAAACC CTCTCGCCCA GGGTCACGCC CGCGTCGACG TAACGTATCC CCGCCTCCCG
            301 GATCCGCCTT CTCCCCGCTT CCGCCCTGCG CGCTAACCCA AGACGGGAGC TCGAGAGCTT
            361 GATCCTGCGC GAGTTTACAG ACTGCCCTCT TCCCTTTTTG CGTAAGGACT AATTCAAACT
            421 TTATGCCGGG GGCGGTGGCT CACGCGTGTA GTCCCAGCTG CTCGGGAGGC CGAGGCGAGA
            481 GTATCGTCTG AGCCTAGGAG TTCGAGGCTA ACCACGGCAA CGCCCCTACT CCTACCCCAG
            541 TCCCTCGCGG GTGGGGCGAG GGATTTCAGG GGGCGGTGGG GGAAGACTTA ACCGAGTTGC
            601 CGTCTTCTGT TTACCAGATC GAAGACCACG ACTCCCAAGA GGTAATGCGT CGGCCCGGGA
            661 CTACATCTCC CGGCATGCAT CGCCGGCGGG TCCGCGAGTT CTCACCATCG CAGCTGGACT
            721 ATTAGCGGGG GCCCTAGCGC TCTTGTGGTT TGTTTCGGCA GTATCTGAAT GTCAGCTTTT
            781 GGCACTTCTG GTCGCTCTGA GCCTCCTACT CGCATTCCTG GAGCAAGGTG CTTTCCAGGC
            841 CTCATGGAGG TGGAGGAGCT GAGGAAGCTC GAGAAGATGG ATAGAAGTGG GAAATGGAGC
            901 TAGCGTACGG GAGAGGGAAA TGGCTGGAAT ATTCGTCAAT CTGTGGTGCT TTACTGGGAG
            961 ATTTGCAGGA GTTCAGAGGA ACGGGGGCAT TGAAATTTCA TAACCTAAAC CGTATCGTGG
           1021 TTTACTCAGA AAAGTTTTGA ACCCGTGATT TCCGGGTTTA AAGCTTGAGG TAAGGACCGG
          1081 GGCGGCGGTG GTCACGGCAG TTAATCCTAG CAGGTTAGAG GTCTAAGGTC AAGG
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB07813_A7I6c.seq check
          >07813_07813_A7I6_pAxCALNL_F1_A02_01_ABI08.ab1
              1 ATAATTTTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA
             61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC
            121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT
            181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT
            241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC
            301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG
            361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG
            421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG
            481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA
            541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC
            601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT
            661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA
            721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC
            781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG
            841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC
            901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGG
            961 TGAACATGCC GAAGCCGTGG GTGAAATGGC ACCCACGCTG AGGGATAGCG GGTGTCGGGG
           1021 ATGAATCTGC TTTGCCCGAG ATGGGGGTCG CGGCAATGGA CTGAATCGGA CACAAGCGGC
          1081 TGCGGCTGCC GTTAGGGACT ACGCCCTTGG GGCCATC
          //
          Primer:
          Sequence file: RDB07813_A7I6d.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl