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pGL4-phADM

Promoter collection, Human ADM promoter

Catalog number RDB07808
Resource name pGL4-phADM
Alternative name U2989-9
Clone info. PCR amplified human ADM promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 10303763 to 10305239(NC_000011.10 ); relatively -1310 to +167, where +1 corresponds to 1 nt of NM_001124.3.
Comment PCR cloning, forward primer: agaaacccacaagcacgaag; reverse primer: cggcacctacctgctaagag.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human adrenomedullin genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human adrenomedullin

          Reference sequence
            

          Distribution information

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          RDB07808 pGL4-phADM DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07807_A3L2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07807_A3L2a.seq check
          >07807_07807_A3L2_pGL4-4174F_G02_20.ab1
              1 ACTGGGGTCC AGACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG
             61 GATCCATGCT CTGAAGCATC AACAACTTAA AGGAATCTAC TCATTGGTAA CACCAATGTC
            121 AAATGATGCG GCATTACAGA ATACAAAAAT GGTATGGCCC TAGAATCACT CTCTTTATAT
            181 AAAATGGGAA CTGGAGGTAT TAACAAAAAA GCCACTAAAC AACATAGATT CCAAATGAAA
            241 GCACAGTGAA TTAGTTGGGT CTTTAGTGAA GCTAAGTAGC ACTATATTAA GAAATGGAGA
            301 TATGCTTAGG TTTATTGGCC ATATTCAACA TCTGCACACA CCCAGGGAGA ATAAAGCCTA
            361 TTCTTTCCTT GTCCTGACCT CACTCCCTCA GGCTGCATGT TCCTTGGGTA ATGAGAAGTC
            421 ACAATCACTA TTCATAGATG TGTGGGGAGT CACTAAAAAT ATATTATTCA CTGTCAATCT
            481 TAGTTTATAT CCAGATACAA CAGGGTACAC TGCTCTTGTA ATGGAATCAG ACTTCTTATT
            541 TTAACAAGAC AAACCAAATC CAATCCACAT TTGAAGATTA TAGGTTTTAA TATAAGAAAA
            601 TGCACTCATT TCTCAAAGAC CCTAGTGAAG CTGTGTTTAA ATGCTCCTAG GTGAACCCCC
            661 TTTGCATCCC AGTGTTCCCA CCCTGACACC CAGAGCCCCT ACCTACCCAA CACAGAATCA
            721 TTTGCTCTGA TAGAACAATG GATCCCTTTT TCTGGAAACA TTGATGGCCA CTCGTCCCTT
            781 GTCCTTGCCT ATATAAAACT CCTACATATA TTAAGAGAAA ACTAAGCAAG AGTTTTGGAA
            841 TCTGCCCCAG GAGACTGCAT CCTGAGTCAC ACGCGTCTTT GTTCTCTTTC TTGTCCCAAA
            901 ACCGTTACCT CAAGTGACAA ATGATCAAAT CTCAAATATA GAATTCAGGG TTTTACAGGT
            961 AGGCATCTTG AGGATTTCAA ATGGTTAAAA GCAACTCACT CCTTTTCTAC TCTTTGGAGA
           1021 GTTTCAAGAG CCTATAGCCT CTAAAACGCA AATCATTGCT AATGGTTGGG GGGGGAGAAA
           1081 CCTTTTCGAT TTTTTTAGAT TCTGCTGGTT GGCCTCTCAG CTAACTACCT TTCTAACAGG
          1141 ATGTATGTCC AGTGGACTGA CACGCTAACT ATCGAAAAGG TA
          //
          Primer: pGL4-136R
          Sequence file: RDB07807_A3L2b.seq check
          >07807_07807_A3L2_pGL4-136R_H02_23.ab1
              1 GCTCTTTAGG TTTTTGGGCA TCTTCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC
             61 TTGGCCGCCG AGGCCAGATC TTGATACTGG CTGTCTCCTC TCAGCGGGGA AGAGTTGTTG
            121 TGTGAGTTTG GGGCGGGCGG CCCGAGGCTT TTATACGCTC CGGGCGGCCG CGGCTCGCCA
            181 ATGAGCTGAA TGGAGTCCTA CACAAACAGG GACATTCCTC GCATTCCTCC CCACCTTCCT
            241 GCCTCATCAA CTCACACCGG ATTGATCCTG ACCCCTTGAC ACTCAGCATT CCTCCGTCTG
            301 AAAAAGCTGG CACACTCCAG CTCACAGAAA AAAAAAAAAG GAAAAAAAAA AGCGCAGTAT
            361 TTCCAGCACC AAATAGAATT TTTTTTTCCC TATCCTCTTC GCACCACTCC TGATTCATAT
            421 CATTTATAAA CACACTGGCA ATTATTTCTT ATTTCTTTTT CGAATAAGTT TGCCCTGTTC
            481 TGTCCACTGA CATACATCCT TTTTAGGAAG TAGGTAGCTG AAGAGGCAAA CAGCAGGAAT
            541 TCCTAAAAAA TTCGAAAAGG TTTCTCCCCC CCAACCCTTA GCAATGATTT GCGTTTTAGA
            601 GGCTATAGGC TCTTGAAACT CTCCAAAGAG TAGAAAAGGA GTGAGTTGCT TTTAACCATT
            661 TGAAATCCTC AAGATGCCTA CCTGTAAAAC CCTGAATTCT ATATTTGAGA TTTGATCATT
            721 TGTCACTTGA GGTAACGGTT TTGGGACAAG AAAGAGAACA AAGACGCGTG TGACTCAGGA
            781 TGCAGTCTCC TGGGGCAGAT TTCCAAAACT CTTGCTTAGT TTTCTCTTAA TATATGTAGG
            841 AGTTTTATAT AGGCAAGGAC AAGGGACGAG TGGCCATCAA TGTTTCCAGA AAAAGGGATC
            901 CATTGTTCTA TCAGAGCAAA TGATTCTGTG TTGGGTAGGT AGGGGCTCTG GGTGTCAGGT
            961 GGGAACACTG GGATGCAAAG GGGGGTTCAC CTAGGAGCAT TTAAACACAG CTTTCACTAG
           1021 GGTCTTTGAG AAATGAGTGC ATTTTTCTTA TATTAAAACC TAATAATCTT TCAAATGTGA
           1081 ATTTGATTTT GCTTTGCTCT TGTTAAAATA AAGAAGTCTG ATCATACAGA GCATGTTACC
          1141 TGTTGTAATC TGGGAATAAT TAAAACCCTA AAAGAAAAAA T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl