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pGL4-phADM (#RDB07808)

Promoter collection, Human ADM promoter

Alternative name U2989-9
Clone info. PCR amplified human ADM promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 10303763 to 10305239(NC_000011.10 ); relatively -1310 to +167, where +1 corresponds to 1 nt of NM_001124.3.
Comment PCR cloning, forward primer: agaaacccacaagcacgaag; reverse primer: cggcacctacctgctaagag.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human adrenomedullin genomic DNA
Depositor|Developer DNA Bank, |
 

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07808 pGL4-phADM DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phADM was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07808).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07807_A3L2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07807_A3L2a.seq check
>07807_07807_A3L2_pGL4-4174F_G02_20.ab1
    1 ACTGGGGTCC AGACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG
   61 GATCCATGCT CTGAAGCATC AACAACTTAA AGGAATCTAC TCATTGGTAA CACCAATGTC
  121 AAATGATGCG GCATTACAGA ATACAAAAAT GGTATGGCCC TAGAATCACT CTCTTTATAT
  181 AAAATGGGAA CTGGAGGTAT TAACAAAAAA GCCACTAAAC AACATAGATT CCAAATGAAA
  241 GCACAGTGAA TTAGTTGGGT CTTTAGTGAA GCTAAGTAGC ACTATATTAA GAAATGGAGA
  301 TATGCTTAGG TTTATTGGCC ATATTCAACA TCTGCACACA CCCAGGGAGA ATAAAGCCTA
  361 TTCTTTCCTT GTCCTGACCT CACTCCCTCA GGCTGCATGT TCCTTGGGTA ATGAGAAGTC
  421 ACAATCACTA TTCATAGATG TGTGGGGAGT CACTAAAAAT ATATTATTCA CTGTCAATCT
  481 TAGTTTATAT CCAGATACAA CAGGGTACAC TGCTCTTGTA ATGGAATCAG ACTTCTTATT
  541 TTAACAAGAC AAACCAAATC CAATCCACAT TTGAAGATTA TAGGTTTTAA TATAAGAAAA
  601 TGCACTCATT TCTCAAAGAC CCTAGTGAAG CTGTGTTTAA ATGCTCCTAG GTGAACCCCC
  661 TTTGCATCCC AGTGTTCCCA CCCTGACACC CAGAGCCCCT ACCTACCCAA CACAGAATCA
  721 TTTGCTCTGA TAGAACAATG GATCCCTTTT TCTGGAAACA TTGATGGCCA CTCGTCCCTT
  781 GTCCTTGCCT ATATAAAACT CCTACATATA TTAAGAGAAA ACTAAGCAAG AGTTTTGGAA
  841 TCTGCCCCAG GAGACTGCAT CCTGAGTCAC ACGCGTCTTT GTTCTCTTTC TTGTCCCAAA
  901 ACCGTTACCT CAAGTGACAA ATGATCAAAT CTCAAATATA GAATTCAGGG TTTTACAGGT
  961 AGGCATCTTG AGGATTTCAA ATGGTTAAAA GCAACTCACT CCTTTTCTAC TCTTTGGAGA
 1021 GTTTCAAGAG CCTATAGCCT CTAAAACGCA AATCATTGCT AATGGTTGGG GGGGGAGAAA
 1081 CCTTTTCGAT TTTTTTAGAT TCTGCTGGTT GGCCTCTCAG CTAACTACCT TTCTAACAGG
1141 ATGTATGTCC AGTGGACTGA CACGCTAACT ATCGAAAAGG TA
//
Primer: pGL4-136R
Sequence file: RDB07807_A3L2b.seq check
>07807_07807_A3L2_pGL4-136R_H02_23.ab1
    1 GCTCTTTAGG TTTTTGGGCA TCTTCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC
   61 TTGGCCGCCG AGGCCAGATC TTGATACTGG CTGTCTCCTC TCAGCGGGGA AGAGTTGTTG
  121 TGTGAGTTTG GGGCGGGCGG CCCGAGGCTT TTATACGCTC CGGGCGGCCG CGGCTCGCCA
  181 ATGAGCTGAA TGGAGTCCTA CACAAACAGG GACATTCCTC GCATTCCTCC CCACCTTCCT
  241 GCCTCATCAA CTCACACCGG ATTGATCCTG ACCCCTTGAC ACTCAGCATT CCTCCGTCTG
  301 AAAAAGCTGG CACACTCCAG CTCACAGAAA AAAAAAAAAG GAAAAAAAAA AGCGCAGTAT
  361 TTCCAGCACC AAATAGAATT TTTTTTTCCC TATCCTCTTC GCACCACTCC TGATTCATAT
  421 CATTTATAAA CACACTGGCA ATTATTTCTT ATTTCTTTTT CGAATAAGTT TGCCCTGTTC
  481 TGTCCACTGA CATACATCCT TTTTAGGAAG TAGGTAGCTG AAGAGGCAAA CAGCAGGAAT
  541 TCCTAAAAAA TTCGAAAAGG TTTCTCCCCC CCAACCCTTA GCAATGATTT GCGTTTTAGA
  601 GGCTATAGGC TCTTGAAACT CTCCAAAGAG TAGAAAAGGA GTGAGTTGCT TTTAACCATT
  661 TGAAATCCTC AAGATGCCTA CCTGTAAAAC CCTGAATTCT ATATTTGAGA TTTGATCATT
  721 TGTCACTTGA GGTAACGGTT TTGGGACAAG AAAGAGAACA AAGACGCGTG TGACTCAGGA
  781 TGCAGTCTCC TGGGGCAGAT TTCCAAAACT CTTGCTTAGT TTTCTCTTAA TATATGTAGG
  841 AGTTTTATAT AGGCAAGGAC AAGGGACGAG TGGCCATCAA TGTTTCCAGA AAAAGGGATC
  901 CATTGTTCTA TCAGAGCAAA TGATTCTGTG TTGGGTAGGT AGGGGCTCTG GGTGTCAGGT
  961 GGGAACACTG GGATGCAAAG GGGGGTTCAC CTAGGAGCAT TTAAACACAG CTTTCACTAG
 1021 GGTCTTTGAG AAATGAGTGC ATTTTTCTTA TATTAAAACC TAATAATCTT TCAAATGTGA
 1081 ATTTGATTTT GCTTTGCTCT TGTTAAAATA AAGAAGTCTG ATCATACAGA GCATGTTACC
1141 TGTTGTAATC TGGGAATAAT TAAAACCCTA AAAGAAAAAA T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles