RDB07738 - pGEM-T Easy-PE40 (as)

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icon below as well as clone information before placing your order.

pGEM-T Easy-PE40 (as) (#RDB07738)

Plasmid clone of Pseudomonas exotoxin A DNA

Clone info.	Plasmid clone of Pseudomonas exotoxin A (exotoxin 40, PE40) DNA.
Comment	Cloned fragment: 5,675,271nt to 5,676,356nt of CP030328.1; relatively 278a.a. to 638a.a. of exotoxin A (AWZ83916.1).
Vector backbone	pGEM-T Easy (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Pseudomonas aeruginosa exotoxin A (exotoxin 40, PE40) genomic DNA
Depositor\|Developer	Hamada, Hirofumi \|

Distribution information

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07738	pGEM-T Easy-PE40 (as)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEM-T Easy-PE40 (as) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07738).

Reference section:

Ishii, K., Nakamura, K., Kawaguchi, S., Li, R., Hirai, S., Sakuragi, N., Wada, T., Kato, K., Yamashita, T., Hamada, H., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.

Tanaka, T., Huang, J., Hirai, S., Kuroki, M., Kuroki, M., Watanabe, N., Tomihara, K., Kato, K., Hamada, H., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07738_A9Gop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 () Region: MCS,insert5' Sequence file: RDB07738_A9Goa.seq
>07738_07738_A9Go_3_M13_-40_C04_07_ABI24.ab1 1 NNNNNNNNNN NNNNANNNGN NTNNCNNTAG GGCGAATTGG GCCCGACGTC GCATGCTCCC 61 GGCCGCCATG GCGGCCGCGG GAATTCGATT GGGGTACCTT ACTTCAGGTC CTCGCGCGGC 121 GGTTTGCCGG GCTGGCTGGC GTAGTCCGGC AGGGCGCTGA TCGCCTGTTC CTTGTCGGGG 181 ATGCTGGACG GGTCGAGGTC GCCGCCGACG TTGCGCGGGT CGGTGGGGAT CGCCGAGGGA 241 ATCACCACGG TGCGCTCGGC CAGCGGCCAG CCGAGAATGG TCTCCAGGCG CCCGCCTTCC 301 TCCTCGGGGC CGGTGATGGC GTCCAGGCGC AGCGGCAGCG GATGGCCGAT CAGCCGTTCG 361 ACCTCGCCCG CCGCCTCCGG CGCGGCCAGG GTCAGGCTGG TGCGGTAGAA GCCCGGCAGG 421 CTCGAGCGCG GCACATAGAC CCGCAGCAGG GCACCGTTGC GGATCCGGCC GCGTGCGTCG 481 GGTTCCTGGT CCTGGGCGTA GCCGTAGGCC AGCGCCGGAT CGCCGGCGAT ATAGAAACCG 541 CGCCAGATCG CGTCGAGGTC CTGGCTGCGC GCGCGCACCC CGCCGAAGAC GATGCTTTGC 601 GCCGCTTCGA GGAAGGTGCC GTGGTAGCCG ACGAACACAT AGCCGCGCTC CTCCAGTTGG 661 CGGTGCGCCT GGAGCAGCCG CTCCACCGTC CAGTTCTGCG TGCCGCGGGT GCTGAAGCTG 721 ACGTCGCCGC CGTCGCCGAG GAACTCCGCG CCAGTGGGAT AGTTGCGCTC CAGCANGGCG 781 TCGCCGCTGT CCGCCGGGNC CGCGCATTCA CCGGCGGCGA NCGGGCANGT CANGNTCACC 841 A //
Primer: Reverse2 () Region: MCS,insert3' Sequence file: RDB07738_A9Gob.seq
>07738_07738_A9Go_3_Reverse2_F04_16_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC NNTTNNGGTG NNNCTATAGA ATACTCAAGC TATGCATCCN 61 ACGCGTTGGG AGCTCTCCCA TATGGTCGAC CTGCAGGCGG CCGCGAATTC ACTAGTGATT 121 CCAAGCTTGG CGGCAGCCTG GCCGCGCTGA CCGCGCACCA GGCTTGCCAC CTGCCGCTGG 181 AGACTTTCAC CCGTCATCGC CAGCCGCGCG GCTGGGAACA ACTGGAGCAG TGCGGCTATC 241 CGGTGCAGCG GCTGGTCGCC CTCTACCTGG CGGCGCGGCT GTCGTGGAAC CAGGTCGACC 301 AGGTGATCCG CAACGCCCTG GCCAGCCCCG GCAGCGGCGG CGACCTGGGC GAAGCGATCC 361 GCGAGCAGCC GGAGCAGGCC CGTCTGGCCC TGACCCTGGC CGCCGCCGAG AGCGAGCGCT 421 TCGTCCGGCA GGGCACCGGC AACGACGAGG CCGGCGCGGC CAACGCCGAC GTGGTGAGCC 481 TGACCTGCCC GGTCGCCGCC GGTGAATGCG CGGGCCCGGC GGACAGCGGC GACGCCCTGC 541 TGGAGCGCAA CTATCCCACT GGCGCGGAGT TCCTCGGCGA CGGCGGCGAC GTCAGCTTCA 601 GCACCCGCGG CACGCAGAAC TGGACGGTGG AGCGGCTGCT CCAGGCGCAC CGCCAACTGG 661 AGGAGCGCGG CTATGTGTTC GTCGGCTACC ACGGCACCTT CCTCGAAGCG GCGCAAAGCA 721 TCGTCTTCGG CGGGGTGCGC GCGCGCAGCC AGGANCTCGA CGCGATCTGG CGCGGTTTCT 781 ATATCGCCNG CGATCCGGCG CTGGCCTACG GCTACGCCCA GGACCANGAA CCCGACGCAC 841 GNNGCCNGNN CCGCAACGGN GCCCTGCTGC NGGTCTATGT GCCGCGCTNG ANCCNTGCCN 901 GGCTTCTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Ishii, K., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
original	Tanaka, T., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

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Other resources in our bank

External Database
Pseudomonas aeruginosa exotoxin A (exotoxin 40, PE40)

Reference sequence