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pGEM-T Easy-PE40 (as)

Plasmid clone of Pseudomonas exotoxin A DNA

Catalog number RDB07738
Resource name pGEM-T Easy-PE40 (as)
Clone info. Plasmid clone of Pseudomonas exotoxin A (exotoxin 40, PE40) DNA.
Cloned fragment: 5,675,271nt to 5,676,356nt of CP030328.1; relatively 278a.a. to 638a.a. of exotoxin A (AWZ83916.1).
Vector backbone pGEM-T Easy (Plasmid)
Selectable markers Amp^r
Gene/insert name Pseudomonas aeruginosa exotoxin A (exotoxin 40, PE40) genomic DNA
Depositor Hamada, Hirofumi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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RDB07738 pGEM-T Easy-PE40 (as) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ishii, K., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
original Tanaka, T., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07738_A9Gop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: MCS,insert5' | Primer: M13_-40 | Sequence: RDB07738_A9Goa.seq
>07738_07738_A9Go_3_M13_-40_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNANNNGN NTNNCNNTAG GGCGAATTGG GCCCGACGTC GCATGCTCCC
   61 GGCCGCCATG GCGGCCGCGG GAATTCGATT GGGGTACCTT ACTTCAGGTC CTCGCGCGGC
  121 GGTTTGCCGG GCTGGCTGGC GTAGTCCGGC AGGGCGCTGA TCGCCTGTTC CTTGTCGGGG
  181 ATGCTGGACG GGTCGAGGTC GCCGCCGACG TTGCGCGGGT CGGTGGGGAT CGCCGAGGGA
  241 ATCACCACGG TGCGCTCGGC CAGCGGCCAG CCGAGAATGG TCTCCAGGCG CCCGCCTTCC
  301 TCCTCGGGGC CGGTGATGGC GTCCAGGCGC AGCGGCAGCG GATGGCCGAT CAGCCGTTCG
  361 ACCTCGCCCG CCGCCTCCGG CGCGGCCAGG GTCAGGCTGG TGCGGTAGAA GCCCGGCAGG
  421 CTCGAGCGCG GCACATAGAC CCGCAGCAGG GCACCGTTGC GGATCCGGCC GCGTGCGTCG
  481 GGTTCCTGGT CCTGGGCGTA GCCGTAGGCC AGCGCCGGAT CGCCGGCGAT ATAGAAACCG
  541 CGCCAGATCG CGTCGAGGTC CTGGCTGCGC GCGCGCACCC CGCCGAAGAC GATGCTTTGC
  601 GCCGCTTCGA GGAAGGTGCC GTGGTAGCCG ACGAACACAT AGCCGCGCTC CTCCAGTTGG
  661 CGGTGCGCCT GGAGCAGCCG CTCCACCGTC CAGTTCTGCG TGCCGCGGGT GCTGAAGCTG
  721 ACGTCGCCGC CGTCGCCGAG GAACTCCGCG CCAGTGGGAT AGTTGCGCTC CAGCANGGCG
  781 TCGCCGCTGT CCGCCGGGNC CGCGCATTCA CCGGCGGCGA NCGGGCANGT CANGNTCACC
841 A
//
Region: MCS,insert3' | Primer: Reverse2 | Sequence: RDB07738_A9Gob.seq
>07738_07738_A9Go_3_Reverse2_F04_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNTTNNGGTG NNNCTATAGA ATACTCAAGC TATGCATCCN
   61 ACGCGTTGGG AGCTCTCCCA TATGGTCGAC CTGCAGGCGG CCGCGAATTC ACTAGTGATT
  121 CCAAGCTTGG CGGCAGCCTG GCCGCGCTGA CCGCGCACCA GGCTTGCCAC CTGCCGCTGG
  181 AGACTTTCAC CCGTCATCGC CAGCCGCGCG GCTGGGAACA ACTGGAGCAG TGCGGCTATC
  241 CGGTGCAGCG GCTGGTCGCC CTCTACCTGG CGGCGCGGCT GTCGTGGAAC CAGGTCGACC
  301 AGGTGATCCG CAACGCCCTG GCCAGCCCCG GCAGCGGCGG CGACCTGGGC GAAGCGATCC
  361 GCGAGCAGCC GGAGCAGGCC CGTCTGGCCC TGACCCTGGC CGCCGCCGAG AGCGAGCGCT
  421 TCGTCCGGCA GGGCACCGGC AACGACGAGG CCGGCGCGGC CAACGCCGAC GTGGTGAGCC
  481 TGACCTGCCC GGTCGCCGCC GGTGAATGCG CGGGCCCGGC GGACAGCGGC GACGCCCTGC
  541 TGGAGCGCAA CTATCCCACT GGCGCGGAGT TCCTCGGCGA CGGCGGCGAC GTCAGCTTCA
  601 GCACCCGCGG CACGCAGAAC TGGACGGTGG AGCGGCTGCT CCAGGCGCAC CGCCAACTGG
  661 AGGAGCGCGG CTATGTGTTC GTCGGCTACC ACGGCACCTT CCTCGAAGCG GCGCAAAGCA
  721 TCGTCTTCGG CGGGGTGCGC GCGCGCAGCC AGGANCTCGA CGCGATCTGG CGCGGTTTCT
  781 ATATCGCCNG CGATCCGGCG CTGGCCTACG GCTACGCCCA GGACCANGAA CCCGACGCAC
  841 GNNGCCNGNN CCGCAACGGN GCCCTGCTGC NGGTCTATGT GCCGCGCTNG ANCCNTGCCN
901 GGCTTCTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ishii, K., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
original Tanaka, T., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

2019.08.19

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